Figure legends

Supplementary Fig. S1. Phase-contrast (a) and scanning electron (b) photomicrographs of cells of strain R160T. Bar, (a) 10µm; (b) 1µm.

Supplementary Fig. S2.Neighbour-joining tree based on rpoB gene sequences showing relationships between the novel strain R160T and other members of the genus Acinetobacter. Pseudomonas aeruginosa PAO1 (NC_002516) was used as an outgroup. Bootstrap values >50% (based on 1000 resamplings) are shown. The scale bar represents 0.2 substitutions per nucleotide position.

Supplementary Fig. S3. Neighbour-joining tree based on gyrB gene sequences showing relationships between strain R160T and other members of the genus Acinetobacter. The sequence of Escherichia coli ATCC 25922 (AB083953) was used as an outgroup. Bootstrap values >50% (based on 1000 resamplings) are shown. The scale bar represents 0.05 changes per nucleotide position.

Supplementary Fig. S4.Thin-layer chromatograms of polar lipids of strain R160T. A, components were revealed by staining the preparations with 5% molybdophosphoric acid in ethanol and heating at 180 °C for 15 min; B, components were revealed by the spraying with ninhydrin reagent, and heating at 110°C for 15 min and cold plate was sprayed with zinzadze reagent. Abbreviations: DPG, diphosphatidylglycerol; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PC, phosphatidylcholine; PL, unknown phospholipid.

Supplementary Table 1.The negative (–) properties of API 20NE, API 32GN and API ZYM tests ofstrain R160T.

Characteristics / R160T
Enzyme activities
N-acetyl-β-glucosaminidase / –
Trypsin / –
Valine arylamidase / –
α-chymotrypsin / –
α-fucosidase / –
α-glucosidase / –
α-mannosidase / –
β-galactosidase / –
β-glucosidase / –
β-glucuronidase / –
Assimilation of / –
3-hydroxybenzoic acid / –
3-hydroxybutyric acid / –
d-maltose / –
d-mannitol / –
d-manose / –
d-melibiose / –
d-ribose / –
d-sorbitol / –
Glycogen / –
Inositol / –
Itaconic acid / –
Lactic acid / –
l-arabinose / –
l-fucose / –
l-rhamnose / –
N-acetyl-glucosamine / –
Potassium 2-ketogluconate / –
Potassium 5-ketogluconate / –
Salicin / –
Sodium malonate / –
Suberic acid / –
Sucrose / –

Supplementary Fig.S1.

Supplementary Fig. S2.

Supplementary Fig. S3.

Supplementary Fig. S4.