multiplex I / sea / Nsea-1 / M18970 / gaa aaa agt ctg aat tgc agg gaa ca / 560 / 55 / FRI913 / 27
Nsea-2 / caa ata aat cgt aat taa ccg aag gtt c
seh / Nseh-1 / U11702 / caa tca cat cat atg cga aag cag / 376 / 55 / FRI137 / 27
Nseh-2 / cat cta ccc aaa cat tag cac c
sec / Nsec-1 / X05815 / ctt gta tgt atg gag gaa taa caa aac atg / 275 / 55 / FRI913 / 27
Nsec-2 / cat atc ata cca aaa agt att gcc gt
tst / Ntst-1 / J02615 / ttc act att tgt aaa agt gtc aga ccc act / 180 / 55 / FRI913 / 27
Ntst-2 / tac taa tga att ttt tta tcg taa gcc ctt
multiplex II / sed / Nsed-1 / M28521 / gaa tta agt agt acc gcg cta aat aat atg / 492 / 55 / FRI1151m / 27
Nsed-2 / gct gta ttt ttc ctc cga gag t
etd / Netd-1 / AB057421 / caa act atc atg tat caa gga tgg / 358 / 55 / TY114 / 67
Netd-2 / cca gaa ttt ccc gac tca g
eta / Neta-1 / M17347 / act gta gga gct agt gca ttt gt / 190 / 55 / A920210 / 27
Neta-2 / tgg ata ctt ttg tct atc ttt ttc atc aac
sek / Nsek-1 / U93688 / atg cca gcg ctc aag gc / 134 / 55 / FRI913 / this work
Nsek-2 / aga ttc att tga aaa ttg tag ttg att agc t
Nsek-3 / AAW36439 / tgc cag cgc tca agg tg
multiplex III / see / Nsee-1 / M21319 / caa aga aat gct tta agc aat ctt agg c / 482 / 55 / FRI918 / 27
Nsee-2 / cac ctt acc gcc aaa gct g
seb / Nseb-1 / M11118 / att cta tta agg aca cta agt tag gga / 404 / 55 / CCM5757 / 27
Nseb-2 / atc ccg ttt cat aag gcg agt
sem / Nsem-1 / AF285760 / cta tta atc ttt ggg tta atg gag aac / 326 / 55 / FRI137 / 27
Nsem-2 / ttc agt ttc gac agt ttt gtt gtc at
sel / Nsel-1 / NC_002745 / gcg atg tag gtc cag gaa ac / 234 / 55 / FRI137 / this work
Nsel-2 / cat ata tag tac gag agt tag aac cat a
seo / Nseo-1 / AF285760 / agt ttg tgt aag aag tca agt gta ga / 180 / 55 / FRI137 / 27
Nseo-2 / atc ttt aaa ttc agc aga tat tcc atc taa c
multiplex IV / sen / Gsen-1 / AF285760 / cgt ggc aat tag acg agt c / 474 / 55 / FRI137 / this work
Gsen-2 / NC_002952 / gat tga tyt tga tga tta tka g
seg / Gseg-1 / AF285760 / tct cca cct gtt gaa gg / 323 / 55 / FRI137 / this work
Gseg-2 / aag tga ttg tct att gtc g
seq / Gseq-1 / AAW36439 / acc tga aaa gct tca agg a / 204 / 55 / Col / this work
Gseq-2 / cgc caa cgt aat tcc ac
sej / Gsej-1 / AF053140 / tca gaa ctg ttg ttc cgc tag / 138 / 55 / FRI1151m / this work
Gsej-2 / gaa ttt tac cay caa agg tac
multiplex V / sei / Gsei-1 / AAW36439/ / cty gaa ttt tca acm ggt ac / 461 / 55 / FRI137 / this work
Gsei-2 / AF285760 / agg cag tcc atc tcc tg
ser / Nser-1 / AB075606 / agc ggt aat agc aga aaa tg / 363 / 55 / FRI1151m / this work
Nser-2 / tct tgt acc gta acc gtt tt
seu / Nseu-1 / AY205306 / aat ggc tct aaa att gat gg / 215 / 55 / FRI137 / this work
Nseu-2 / att tga ttt cca tca tgc tc
sep / Gsep-1 / NC_002745 / gaa ttg cag gga act gct / 182 / 55 / N315 / this work
Gsep-2 / ggc ggt gtc ttt tga ac
multiplex VI / agr 1 – 4 / pan agr / atg cac atg gtg cac atg c
agr-1 / agr1 / X52543 / gtc aca agt act ata agc tgc gat / 439 / 55 / Col / 35
agr-2 / agr2 / AF001782 / tat tac taa ttg aaa agt gcc ata gc / 572 / 55 / N315 / 35
agr-3 / agr3 / AF001783 / gta atg taa tag ctt gta taa taa tac cca g / 320 / 55 / TY114 / 35
agr-4 / agr4 / AF288215 / cga taa tgc cgt aat acc cg / 657 / 55 / A920210 / 35
single PCR / 16SrRNA / 16SrRNA-1 / gta ggt ggc aag cgt tat cc / 228 / 58 / 24
16SrRNA-2 / cgc aca tca gcg tca g
gyrase / gyr-1 / agtaca tcg tcg tat act ata tgg / 281 / 55 / 37
gyr-2 / atc acg taa cag ttc aag tgt g
mecA / mecA-1 / NC_002745 / aaa atc gat ggt aaa ggt tgg c / 533 / 55 / N315 / 45
mecA-2 / agt tct gca gta ccg gat ttg c
pvl / Npvl-1 / AB006796 / atc att agg taa aat gtc tgg aca tga tcc a / 433 / 55 / ATCC49775 / 27
Npvl-2 / gca tca ast gta ttg gat agc aaa agc
sequen-cing / sem / sems-1 / gat agr saw rtt taa wta tag gag aaa ta / 780 / 56.8 / this work
sems-2 / tcc ttt wct aag tta tga ttg aa
spa / spa-1113f / taa aga cga tcc ttc cgt gag c / variable / 60 / 22
spa-1514r / cag cag tag tgc cgt ttg ctt
Suppl. table 1: Oligonucleotide primers and control strains used for toxin gene detection.