From,

From,

DR.ASHWINI.AMBALPADY

M.D. (Ayu) SCHOLAR

Dept of P.G.Studies in Rasa Shastra,

S.D.M.College of Ayurveda,

Kuthpady, Udupi-574118

To,

THE REGISTRAR,

RajivGandhiUniversity of Health Sciences

Karnataka,Bangalore.

Through,

THE PRINCIPAL,

S.D.MCollege of Ayurveda.

Kuthpady, Udupi-574118

Respected Sir,

Sub: Submission of completed proforma for registration of synopsis for Dissertation

I request you to kindly register the below mentioned subject against my name for the submission of dissertation to Rajiv Gandhi University of Health Sciences,Karnataka, Bangalore, for partial fulfilment of M.D. (Ayu) in Rasa Shastra.

TITLE OF DISSERTATION:

“AN EXPERIMENTAL EVALUATION OF IMPACTS OF MASHA AS AN APATHYA IN THE HAEMATINIC ACTIVITY OF LOHA BHASMA”

Here with I am enclosing completed proforma for registration of subject for dissertation.

Thanking You,

Place: Udupi

Yours faithfully,

Date:

(DR. ASHWINI.AMBALPADY)

1) Name of Candidate: DR.ASHWINI. AMBALPADY

M.D. (Ayu) Scholar

DEPT OF P.G. STUDIES IN RASA SHASTRA,

S.D.M.COLLEGE OF AYURVEDA,

KUTHPADY, UDUPI-574118

Permanent Address: DR.ASHWINI AMBALPADY

D/O A MADHAVA ACHARYA

ANURAGA,

KUNJAGUDDE, AMBALPADY

UDUPI -576103

KARNATAKA

2) Name of the Institution: S.D.M.COLLEGE OF AYURVEDA,

KUTHPADY, UDUPI-574118

3) Course of Study & Subject: M.D. (Ayu) IN RASA SHASTRA

4) Date of Admission of Course: 30th May 2008

5) Title of the Topic:

“AN EXPERIMENTAL EVALUATION OF IMPACTS OF MASHA AS AN APATHYAIN THE HAEMATINIC ACTIVITY OF LOHA BHASMA”

6) Brief resume of the intended work

6.1 Need for the study:

Anaemia, which means “Lacking in blood” is among the most common diseases, affecting human beings. It denotes a shortage of rich red blood cells and colouring matter called Haemoglobin. The vast majority of anaemia’s are due to the failure of Haemoglobin synthesis as a result of iron deficiency.

The agents required in the formation of blood and used for the treatment of Anaemia’s are called haematinics.Loha is one such a drug explained under dhatu varga in Rasa Shastra ,administered in the form of Bhasma.

According to classical text books of Rasa Shastra, intake of some food factors is contraindicated during the administration Loha Bhama like Masha, Kushmanda1 etc.and named them as Apathya’s. By considering above factors, the study is planned to evaluate the impact of Masha as an apathya in the haematinic activity of Loha Bhasma.

6.2 Review of Literature:

1) Preparation of Loha Bhasma is explained in Rasa Ratna Samuchchaya.2

2) Apathya’s during the administration of Loha Bhasma is explained by Acharya Madhava1.

3) Information regarding the experimental study is available in Fundamental of Experimental Pharmacology.3

4) Explanation about Iron Deficiency Anaemia, Haemopoisis are present in Robbins Pathologic Basis of Disease.4

6.3 Previous work done:

1. Bhattacharya RC (1965). IPGTR&RA, GAU, Jamnagar, has done work on “Pandu Roga par Paaradaamruta tikshna Loha kaaryaveekshana”.5

2 Pathak KM (1979).Govt. AyurvedicCollege, BFUHS, Faridkot, has done work on “Loha Bhasma nirmana Evam Rakta-Kanoon par prabhava”.5

3. Bansal V (2001).National Institute Of Ayurveda, RajastanUniversity, Jaipur, has done work on “Loha Sevane Varganiyanam”.5

4. Sangolge Bandappa (2004).SDMCA, Udupi, RGUHS,Karnataka, has done work on “Preparation of Loha Bhasma and its clinical study in Pandu Roga w.s.r. to Iron Deficiency Anaemia”.5

6.4) Objectives of study:

A) Literary Study.

B) Pharmaceutical study.

C) Experimental study.

1. Experimental evaluation of haematinic effect of the Loha Bhasma.

2. Experimental evaluation of haematinic effect of Loha Bhasma along with Masha as an Apathya.

7) Materials and Methods:

7.1 Source of data:

A)Literary Study –Information will be collected from Ayurvedic classical text books, modern text books & relevant research literatures.

B)Pharmaceutical study-

a)Raw drugs will be collected from S.D.M Ayurvedic Pharmacy, Udupi.

b) Preparation of Loha Bhasma at S.D.M Ayurvedic Pharmacy.Udupi.

C) Experimental Study-

1) Albino Rats will be taken randomly from SDMCA Udupi Animal house for the study and grouped in to 4, each groupconsisting of 8 rats.

2) Haematinic effect of Loha Bhasma will be studied on Albino Rats.

3) Haematinic effect of Loha Bhasma along with Masha as an Apathya will be studied on Albino Rats.

7.2 Method of collecting of data(including sampling procedures if any)

.

Experimental Evaluation:

1) / Sample / 32 maleAlbino Rats will be selected randomly
2) / Inclusive criteria / 1) Healthy male Albino Rats will be considered.
2) Weighing about 150-200 grams.
3) / Exclusive criteria / 1) Less than 150 grams more than 200 grams.
2) Diseased rats.
3) Rats which are under trial of other experiments
4) / Grouping / Each group having 8 Rats and will be kept in separate cages.
Group 1 - Control Group - 8 Rats
Group 2 - Test Group -8 Rats
Group 3 - Test Group -8 Rats
Group 4 - Test Group -8 Rats
5) / Intervention / Rat dose will be calculated on the basis of human dose by using standard conversion method.
6) / Mode of administration / Oral
7) / Duration / 96 hours
8) / Instrument used / Sahlis haemoglobin meter.
9) / Comparison / All groups are compared
10) / Assessment criteria / 1)Complete blood Count
2)Peripheral smear study
3)Reticulocyte count
11) / Statistical test / ANOVA

7.3) Dose the study require any investigation or Intervention to be conducted on patients or other humans or animals? If so please describe briefly.

Since this is an Experimental Study on male Albino Rats, that are maintained on Standard Laboratory, Foods and water, clinical study is not required.

7.4) Has ethical clearance been obtained from your Institution in case of 7.3?

YES, Concurrence has been obtained from the ethical committee of S.D.M.C of

Ayurveda, Udupi

8) References:

1)Madhava.Ayurveda Prakasha Arthavidyotini-Suspashtartha Prakashini-Sanskrit-Hindi Vyakhyopetaha.4th edition.Varanasi: ChaukhambaBharatiAcademy; 1994.Pp-504;

P-393.

2) Vaagbhata.Rasa Ratna Samuchchaya.3rd edition.Varanasi:Chaukhamba Sanskrita Bhavana; 2006.Pp-418; P-63.

3)Ghosh M N.Fundamentals of Experimental Pharmacology.4 th edition. Kolkata: Hilton & Company;2008.Pp-278; P-230.

4)Cotran Ramzi S, Kumar Vinay, Robbins Stanley L. Robbins pathologic Basis of disease.

5th edition. Bangalore: Prism books (PVT) LTD; 1994. Pp – 1400; P- 586.

5) Baghel M S. Research in Ayurveda. 2nd edition. Jamnagar: Mridu Ayurvedic publication & sales; 2005. Pp- 381; P- 29,273,124,202.

9) Signature of the Candidate :

10) Remarks of the Guide :

11) Name and Designation :

11.1) Guide : DR.SUJATHA.K

M.D (Ayu)

ASSISTANT PROFESSOR.

Dept of P.G.Studies in Rasa Shastra,

S.D.M.College of Ayurveda,

Kuthpady, Udupi-57411

11.2) Signature :

11.3) Co –Guide :DR.SHRIKANTH.P

M.D (Ayu)

LECTURER

Dept of P.G.Studies in Dravyaguna

S.D.M.College of Ayurveda,

Kuthpady, Udupi-574118

11.4) Signature :

11.5) Head of the Department : DR.SEKHAR REDDY.P

M.D(Ayu)

ASSISTANT PROFESSOR.& H.O.D

Dept of P.G.Studies in Rasa Shastra& Bhaishajya

Kalpana

S.D.M.College of Ayurveda,

Kuthpady, Udupi-57411

11.6) Signature :

12)

12.1) Remarks of the Chairman :

And Principal

12.2) Signature :