Diversity and Distribution of Ammonia-Oxidizing Archaea in the Seasonally Frozen Soils

Diversity and Distribution of Ammonia-oxidizing Archaea in the Seasonally Frozen Soils in Northeastern China

Chun-Hong Chen, Da-Wen Gao*,Yu Tao

State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China

*Corresponding author, email: ; Tel: 6-451-86289185; Fax: 86-451-86289185

TableS1 Pairwise differences within and between samples. Mean pairwise sequence differences within samples (πx) are on the diagonal in bold type. Mean pairwise sequence differences betweensamples (πxy) are above the diagonal. Pairwise population FST values are below the diagonal in italic type. UHS=unfertilized hydric soil, UMS=unfertilized mesic soil, FHS=fertilized hydric soil, FMS=fertilized mesic soil

Table S2Pairwise differences within and between different sample properties. Mean pairwise sequence differences within samples (πx) are on the diagonal in bold type. Mean pairwise sequence differences between samples (πxy) are above the diagonal. Pairwise population FST values are below the diagonal in italic type. The number with asterisk shows a special small FST value between a hydric soil and a mesic soil, which is probably a result of the fertilization impact. UHS=unfertilized hydric soil, UMS=unfertilized mesic soil, FHS=fertilized hydric soil, FMS=fertilized mesic soil

Figure Lengends

Figure S1 Rarefaction curves indicating the relative richness of OTUs based on archaealamoAsequences from frozen soil samples. OTUswere defined as groups of sequences differing by <5% at the DNA level. The small figure in the upside is a zoom of the curves of A05 and A09

Table S1

A01 / A02 / A03 / A04 / A05 / A06 / A07 / A08 / A09
A01 / 47.38 / 124.25 / 124.75 / 130.40 / 130.37 / 120.85 / 118.90 / 123.16 / 121.17
A02 / 0.77 / 9.11 / 127.22 / 84.05 / 112.95 / 114.72 / 89.16 / 86.02 / 103.20
A03 / 0.49 / 0.65 / 80.89 / 124.53 / 118.25 / 128.08 / 114.73 / 117.29 / 127.03
A04 / 0.50 / 0.45 / 0.34 / 82.78 / 88.00 / 125.23 / 102.93 / 91.87 / 116.62
A05 / 0.55 / 0.65 / 0.37 / 0.14 / 69.02 / 131.60 / 115.42 / 99.93 / 125.30
A06 / 0.30 / 0.43 / 0.21 / 0.18 / 0.28 / 121.64 / 120.72 / 119.96 / 127.42
A07 / 0.41 / 0.42 / 0.24 / 0.14 / 0.30 / 0.11 / 93.55 / 92.77 / 112.08
A08 / 0.47 / 0.47 / 0.30 / 0.10 / 0.24 / 0.15 / 0.05 / 82.21 / 111.32
A09 / 0.62 / 0.74 / 0.50 / 0.45 / 0.55 / 0.35 / 0.38 / 0.43 / 44.89

Table S2

UHS
A01, A02, A03 / UMS
A04, A05 / FHS
A06, A07 / FMS
A08, A09
UHS
(A01, A02, A03) / 97.55 / 111.79 / 114.18 / 108.56
UMS
(A04, A05) / 0.19 / 83.99 / 117.22 / 99.43
FHS
(A06, A07) / 0.07 / 0.15 / 115.61 / 110.72
FMS
(A08, A09) / 0.13 / 0.12 / 0.07* / 90.99

Figure S1