Brucellosis
OIE Reference Laboratory Reports
Activities in 2010
Name of disease (or topic) for which you are a designated OIE Reference Laboratory: / BrucellosisAddress of laboratory / Brucellosis Laboratory, Bacteriology Division, National Veterinary Research and Quarantine Service (NVRQS), Ministry of Food, Agriculture, Forestry, and Fisheries (MIFAFF), 480 Anyang 6-dong, Manan-gu, Anyang-si, Gyeonggi-do, Republic of Korea
Tel.: / (+82-31) 467.17.65
Fax: / (+82-31) 467.17.78
e-mail address: /
website: / http://www.nvrqs.go.kr
Name of Head of Laboratory (Responsible Official): / Joo- Ho Lee
Name of OIE Reference Expert: / Suk-chan Jung
Name of writer of this report
(if different from above):
Part I: Summary of general activities related to the disease
1. Test(s) in use/or available for the specified disease/topic at your laboratory
Test / For / Specificity / TotalRBT / Antibody / Whole cell / 1,825
SAT / Antibody / Whole cell / 1,763
ELISA / Antibody / LPS / 213
FPA
/ Antibody / OPS / 1,390PCR
/ DNA / Brucella spp. / 672Molecular typing(MLVA)
/ DNA / Brucella strains / 73Bacterial culture
/ Isolation / Brucella spp. / 672Brucella abortus was first isolated from chinese water deer among wild animal. Also we gained 73 Brucella canis from Veterinary Service Laboratory in Gyeongbuk province.
A total of 1,763 sera obtained from cattle (213), pig (970), deer (420) and goat (160) were examined for brucellosis surveillance in Korea.
2. Production and distribution of diagnostic reagents
Type of reagent / Amount supplied nationally(including for own use) / Amount supplied to other countries
RBT antigen (B. abortus) / 1,000 dose
RBT antigen (B. canis M-strain) / 1,000 dose
SAT antigen (B. abortus) / 69,700 dose
CFA (B. abortus) / 1,000 dose
PCR test kit(BASS PCR) / 5,000 test / 480 test (China)
Part II: Activities specifically related to the mandate
of OIE Reference Laboratories
3. International harmonisation and standardisation of methods for diagnostic testing or the production and testing of vaccines
We are participated to the ring trial, among OIE reference labolatories, to evaluate of FPA kit from Argentina.
Diagnostic antigens produced in our laboratory were compared with antigens gained from other reference laboratory and international reference standards.
4. Preparation and supply of international reference standards for diagnostic tests or vaccines
We provided 480 PCR (B. abortus) test kit to Yabian University, China.
5. Research and development of new procedures for diagnosis and control
We are collaborating with Mrs J A Stack`s reference laboratory (VLA, UK) for development of differential diagnosis method using specific antigens for Brucellosis. The title is 『Differential diagnosis of bovine brucellosis using specific antigens..
For canine brucellosis DNA vaccine, our laboratory is searching for new antigen from Brucella canis cell envelope. Now we found 12 candidate antigens.
Studies on development of a rapid genetic differential diagnostic method and molecular epidemiological analysis for Brucella isolates.
Also we are collaborating with universities in Korea for below topics 『Studies on pathogenesis of bovine brucellosis throughunderstanding of Brucella abortus-host cell interaction.』 and 『Surveillance of Brucellosis in Wild animals and Rodents.
6. Collection, analysis and dissemination of epizootiological data relevant to international disease control
None
7. Provision of consultant expertise to OIE or to OIE Members
We consulted with National Institute of Animal Health, Thailand and Institute of Veterinary Medicine, Mongol for diagnostic methods and eradication program of brucellosis. We are planning to MOU for mutual cooperation in 2011.
8. Provision of scientific and technical training to personnel from other OIE Members
We provided technical training to veterinary officers (20 persons) from Laos, Myanmar, Vietnam, Cambodia, Thailand, Philippines, Indonesia and Malaysia.
9. Provision of diagnostic testing facilities to other OIE Members
None
10. Organisation of international scientific meetings on behalf of OIE or other international bodies
None
11. Participation in international scientific collaborative studies
For the joint research project on development of differential diagnosis for bovine brucellosis between our lab (NVRQS) and VLA (UK), one of researchers dispatched to VLA for 2 months.
12. Publication and dissemination of information relevant to the work of OIE (including list of scientific publications, internet publishing activities, presentations at international conferences)
¡ Presentations at international conferences and meetings
Moon Her, Sung Il Kang, Jong Wan Kim, Ji Yeon Kim, Bok Kyung Ku, In Yeong Hwang, Dong Hee Cho, Suk Chan Jung, Han Sang Yoo. Comparison of the isolation rates of Brucella abortus from intra-organs of cattle and environmental specimens in farms. 2010 International Symposium of Climate change and relevant diseases in human and animals. Seoul, Korea, April 22, 2010.
Sung Il Kang, Moon Her, Jong Wan Kim, Ji Yeon Kim, Bok Kyung Ku, In Yeong Hwang, Suk Chan Jung. Discrimination of Brucella canis using real-time PCR assay with Hybprobe. 2010 International Symposium of Climate change and relevant diseases in human and animals. Seoul, Korea, April 22, 2010.
Ji Yeon Kim, Ko Kyung Yuk, Moon Her, Sung Il Kang, Jong Wan Kim, Dong Hee Cho, Bok Kyung Ku, In Yeong Hwang, Suk Chan Jung. Isolation of cross-reactive pathogens for bovine brucellosis and western blot patterns using outer membrane protein of Brucella abortus. 2010 International Symposium of Climate change and relevant diseases in human and animals. Seoul, Korea, April 22, 2010.
Seung Bin Cha, Nabin Rayamahji, Won Jung Lee, Min Kyung Shin, Myung Hwan Jung, Yu Mi Roh, Kyung Yuk Ko, Jong Wan Kim, Han Sang Yoo. Proteomic analysis of Brucella abortus EZ-Tn(TM) pMOD-3_MCS_ transposon mutants cell envelope proteins. 2010 International Symposium of Climate change and relevant diseases in human and animals. Seoul, Korea, April 22, 2010.
Yun-Mi Ha, Jong Wan Kim, Ko Kyung Yuk, Sung Il Kang, Ji Yeon Kim, Moon Her, Suk Chan Jung. The usefulness of recombinant Omp31 for diagnosis of canine brucellosis. 2010 International Symposium on Outbreaks of Devastating and Emerging Animal Diseases. Gyeong Ju, Korea October 21~22, 2010.
Jong Wan KIM, Ji Yeon KIM, Yun-Mi HA, Kyung Yuk KO, Sung-Il Kang, Moon HER, Bok Kyung KU, In-Young Hwang, Suk-Chan JUNG. Immunoproteomic analysis of cell envelope proteins of B. canis for vaccine development. Prato Conference on the Pathogenesis of Bacterial Disease of Animals. Prato, Italy. October 6-9, 2010.
Moon Her, Sung-Il Kang, Jong Wan Kim, Ji Yeon Kim, Suk-Chan Jung. One step multiplex PCR assay for the differentiation of all Brucella species. 63rd Annual Brucellosis Research Conference. Chicago, USA. December 4-5, 2010.
S. Kang, M. Her, J. Kim, J. Kim, K. Ko, B. Ku, Y. Jang, I. Hwang, S. Jung. Development of the improved multiplex PCR for the differential identification of Brucella species. Conference of research worker in animal diseases. Chicago, USA. December 4-6, 2010.
J. Kim, K. Ko, D. Cho, M. Her, J. Kim, S. Kang, B. Ku, I. Hwang, Y. Jang, S. Jung. Comparison of agreement rate between developed C-ELISA and commercial C-ELISA assay for serological diagnosis of bovine brucellosis. Conference of research worker in animal diseases. Chicago, USA. December 4-6, 2010.
K. Ko, J. Kim, J. Kim, H. Lee, J. Park, M. Her, S. Kang, Y. Jang, S. Jung. Identification of immunogenic insoluble proteins of Brucella abortus separated by two-dimentional electrophoresis to develop specific antigens required for Brucellosis diagnosis. Conference of research worker in animal diseases. Chicago, USA. December 4-6, 2010.
¡ Scientific publications in peer-reviewed journals
Cho Donghee,Nam Hyangmi,Kim Jongwan,Heo Eunjeong,Cho Yunsang,Hwang Inyeong,Kim Jaemyung,Kim Jongman,Jung Sukchan andMore Simon. 2010. QUANTITATIVE ROSE BENGAL TEST FOR DIAGNOSIS OF BOVINE BRUCELLOSIS. Journal of Immunoassay and Immunochemistry, 31:2, 120 – 130
Her M, Cho DH, Kang SI, Lim JS, Kim HJ, Cho YS, Hwang IY, Lee T, Jung SC, Yoo HS. 2010. Outbreak of brucellosis in domestic elk in Korea. Zoonoses Public Health. 57(3):155-61.
Her M, Cho DH, Kang SI, Cho YS, Hwang IY, Bae YC, Yoon H, Heo YR, Jung SC, Yoo H. 2010. The development of a selective medium for the Brucella abortus strains and its comparison with the currently recommended and used medium. Diagn Microbiol Infect Dis. 67(1):15-21.
Her M, Kang SI, Kim JW, Kim JY, Hwang IY, Jung SC, Park SH, Park MY, Yoo H. 2010. A Genetic Comparison of Brucella abortus Isolates from Animals and Humans by Using an MLVA Assay. J Microbiol Biotechnol. 20(12):1750-5.
Yoon H, Moon OK, Her M, Carpenter TE, Kim YJ, Jung S, Lee SJ. 2010. Impact of bovine brucellosis eradication programs in the Republic of Korea. Prev Vet Med. 95(3-4):288-91.
Suk Chan Jung, Moon Her, Jong Wan Kim, Ji Yeon Kim, In Yeong Hwang, Bok Kyung Ku, Yong Ho Park, Han Sang Yoo. 2010. Bovine Brucellosis in Korea. The Journal of Zoonoses. 1(1):1-15.
Eun-Jeong Heo, Jong Wan Kim, Dong Hee Cho, Hyang Mi Nam, Yun Sang Cho, In Yeong Hwang, Sung-Il Kang, Suk Chan Jung, Jin San Moon, Sung Hwan Wee. 2010. Serological survey of brucellosis for pigs from slaughter house in South Korea. Kor. J. Vet. Publ. Hlth., 34 (3): 255-258.
¡ Other communications
None
13. Inscription of diagnostic kits on the OIE Register
i) Did you participate in expert panels for the validation of candidate kits for inscription on the OIE Register? If yes, for which kits?
–
ii) Did you submit to the OIE candidate kits for inscription on the OIE Register? If yes, for which kits?
–
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Annual reports of OIE Reference Laboratories and Collaborating Centres, 2010 5