Supplementary Table S1. Primers for Q-PCR analysis

Primers / B. napus EST # / Annotated Function in Arabidopsis / Primer Sequence (5' to 3') / log2values for
B. villosa relative to B. napus
Up-regulated general genes
EE565903-F / EE565903 / Double mutants defective in pollen germination. / TGAGACCCTCTGGACCAATC / 1.92
EE565903-R / CAATGGATTTTGCTTGCCTT
JCVI_20644-F / JCVI_20644 / Potential natural antisense gene / TCTGCAGGATGTATAAGCTTG / 1.85
JCVI_20644-R / TGGATCTCATAGGACGGCTC
EL588775-F / EL588775 / Homeobox-leucine zipper (HD-ZIP IV) family / ACGTCTAATTGCATCGCTCC / 2.11
EL588775-R / CAGTTCTTCCATCGCAGTCA
EV199010-F / EV199010 / Cell wall cellulose accumulation and pectin linking. / CTCGTGTGATCTCTCGTGGA / 2.17
EV199010-R / CCCAGCTCCATCTTTGGTTA
EV133121-F / EV133121 / Myb-related protein similar to CPC / AATGGGTGGTCTGGTCTCAG / 6.93
EV133121-R / TGGAACAGCAAACTCGAATG
EV055366-F / EV055366 / Trichome and root hair patterning. / TGGATAACACTGACCGTCGT / 1.55
EV055366-R / ACCTATCACCGACAAGTCTA
EF1alphaF / FJ529180 / Brassica napus elongation factor 1 alpha (EF1alpha.1) / CCCATTCGTCCCCATCTCTGGA
EF1alphaR / ACGGAGGGGCTTGTCCGAGG
Down-regulated general genes
CO750296-F / CO750296 / Cuticle membrane and wax biosynthesis / GTTGTGGCAGTACACGATGG / -5.74
CO750296-R / GCCAATCTTGGGAGAATCAA
EV132967-F / EV132967 / Super sensitive to ABA and drought 2 / GCAGCTAGCAATGGACAACA / -3.70
EV132967-R / TCAACAGATGCTTCAGCAGG
ES977072-F / ES977072 / Enzyme putatively involved in trehalose biosynthesis / AGGTTAATGCATGGTTTGGC / -4.35
ES977072-R / GCTCATAACTCCCAAGGCAA
EH426427-F / EH426427 / XI myosin protein family involved in root hair growth and trichome length. / AACTCTGTCCGGTGCTCAGT / -5.35
EH426427-R / CGTCCAAAAGGAAAGAGCTG
JCVI_17109-F / JCVI_17109 / Mutants display cytoskeletal defects / CCTTTTTACAACGCCCTGAC / -3.76
JCVI_17109-R / ATTCAGGTTGGGCAATTGTT
JCVI_1858-F / JCVI_1858 / Dynamin-like protein related to phragmoplastin / GTCGTCTGGGTGGTTGAGAT / -2.38
JCVI_1858-R / TCCACTGTACCCTTCCGTTC
Calcium regulator genes
Calcium1-F / EV090632 / Calcium-dependent lipid-binding / GTGAACGACGCTCCATCAGCT / 2.12
Calcium1-R / ACAGGCAGTTGCAGAAGAC
Calcium2-F / JCVI_11127 / Calcium-dependent phosphotriesterase superfamily protein / CTAAGGATGTTCATACTGAGA / -1.75
Calcium2-R / AGCTTGTGAAGGTATTAGAA
Calcium3-F / JCVI_20563 / Calcium-binding EF-hand family protein / CTCGGTCACTCACGACGACA / 2.96
Calcium3-R / GCTAACCGTGGTCTCATGA
Calcium4-F / EV134295 / Calcium exchanger 7 / TCCGCTATTCAACACGGTGA / 3.23
Calcium4-R / TCGTTGCTTGAGACACTAGG
Calcium5-F / CX195307 / Member of Calcium Dependent Protein Kinase / AGCATCAAGCTAATGAAGGA / -2.80
Calcium5-R / ACGTCACGGACTCAGAATTC
Trichome regulatory genes
GL1-F / HQ162473.1 / Myb-like protein for induction of trichome development / ACTGGGCTGAAGAGGTGTGGA
GL1-R / GAGATGAGTGTTCCAGTGA
GL2-F / EU826521.1 / Homeodomain protein affecting epidermal cell identity / CGCTGGCCGGGAGAAAGAGC
GL2-R / GGAGGTTTTTTCTGGATGAA
EGL3-F / Bra027653 / Functionally redundant with GL3 and TT8 / ACATTCAATGGAGTTACGGA
EGL3-R / AGAGATTCGTAAAGCTCTCT
TTG1-F / EF175932.1 / Involved in trichome and root hair development / CTCTGGGAGGTCAACGAA
TTG1-R / ATGCTGCACGTGCCTAAC
TRY-F / EE451172 / R3 MYB - overexpression leads to glabrous leaves / CATCACTCCTCTTCTCACA
TRY-R / TGTGGTGGGGAAGAAAACAGA

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