Supplementary Materials
Table S1. Sequences of PCR primers for validation of insertion of mMoshan elements in peach
Name / Forward primer (5’-3’) / Reverse primer (5’-3’)MTH1 / ATCAACTGAGGTGGCTACGT / GGGACCAAACTGATGAAGGC
MTH2 / ACAGGGACATCTTAGCCTGC / CGTGCTCCCATTTAAAGGCT
MTH3 / TGGTGGTACTTTGGACGTGT / CAACCTTCACACCAAGCGAC
MTH4 / TGGCTCCTGAATGATTGGCT / CCTTGCCATTGTTGACCCAT
MTH5 / AGGATTCGAAGGTTCCAGCA / AGTGAAAGTGAGATCGGGCA
MIRA1 / GATGGACACGTGGCAGAATC / ACTCCCCTGATTTGGACTGA
MIRA2 / GGAGACACAGAGAGAACACCA / TCGCGCTGTCTGATTGTCTA
MIRA3 / TTGATTCTCCGAACCGACCT / GGGCAGAGGAAGAGGCTTAT
JX1 / TCGAACAAGCCATGGTTCAA / TATCGCAGTTGAGGAGGACC
JX2 / TGGTAACCACATGCGCTTTC / GGGACAAAGTGGCTGAAGAA
Fig. S1. Protein sequences alignment of transposases encoded by peach aMoshan elements. The transposase genes with GDR accession numbers ppa018763m, ppa018881m, ppa016808m, ppa015420m, ppa016227m, and ppa025038m correspond to the aMoshan elements, Pp_aMoshan_s1-1, Pp_aMoshan_s1-2, Pp_aMoshan_s7-1, Pp_aMoshan_s7-2, Pp_aMoshan_s7-3, and Pp_aMsohan_s2-1, respectively. The degree of sequence identity is shown by height and color (green > yellow > red). White, black, and gray colored blocks indicate the degree of sequence similarity, and sequence gap is marked with solid line. The alignment was conducted using the Geneious program.
Fig. S2. Length distribution of the Moshan elements in peach and P. mume.
Fig. S3. Analysis of the mMoshan element located in the promoter region of PpCHI gene in different varieties of peach. M, DNA ladder; 1, ‘Hongyetao’; 2, ‘Prunus mira Koehne’; 3, ‘Prunus davidiana Franch’; 4, ‘Prunus kansuensis Rehd’; 5, ‘Prunus ferganensis’; 6, ‘Mantianhong’; 7, ‘Baifeng’; 8, ‘Shuguang’; 9, ‘Hongbaihua’; 10, ‘Dahongpao’; 11, ‘Jinxiang’; 12, ‘Mingxing’; 13, ‘Xinfeng’; 14, ‘Jinxiu’; 15, ‘Baihuashuimi’;16, ’Nanshantiantao’; 17, ‘Dajiubao’; 18, ‘Huaguang’; 19, ‘Hujingmilu’; 21, ‘Huachun’; 22, ‘Dubaifeng’; 23, ‘Wujingbaifeng’; 24, ‘Dazhenbaochiyue’.
Fig. S4. Validation of new integration events of Moshan elements in peach accessions resequenced. Primer pairs were shown above the line, and their sequences were listed in Table S1. The numbers represent different accessions that were subjected to resequencing. 1, P. mira; 2, Dahongpao; 3, Jinxiang; 4, Mantianhong; 5, Mingxing.
Fig. S5 Terminal inverted repeats (TIRs) of the Moshan elements in different plants.
Fig. S6. Conserved regions in the tMoshan and mMoshan elements. The red arrows indicate terminal inverted repeats. The pink square indicates DNA sequences with no similarity to the tMoshan elements. The green square indicates a MuDR-derived fragment.
Fig. S7. Conserved regions in the aMoshan and tMoshan elements. The red arrows indicate terminal inverted repeats. The empty squares represent exons of the transposase genes, while the colored squares in the tMoshan elements indicate DNA regions with no similarity to the aMoshan elements.