Electronic Supporting Material on the Microchimica Acta Publication Entitled

Electronic Supporting Material on the Microchimica Acta publication entitled

A versatile DNA detection scheme based on the quenching of fluorescent silver nanoclusters by MoS2 nanosheets: Application to aptamer-based determination of hepatitis B virus and of dopamine

Fei Qu1, 2*, Yining Liu1, 2, Rongmei Kong1, 2, Jinmao You1, 2, 3*

1The Key Laboratory of Life-Organic Analysis, Qufu Normal University, Qufu, Shandong 273165, China

2The Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University, Qufu, China

3Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China

Fig. S1 UV−vis absorption spectrum and fluorescence spectra of AgNCs (a) and TEM images of AgNCs (b).

Fig. S2 UV-vis absorption spectrum of MoS2 (Inset: photograph of the MoS2 suspension) (a) and TEM image of MoS2 nanosheets (b).

Fig. S3 Fluorescence spectra of AgNCs with addition of different volumes of MoS2.

Fig. S4 UV-vis absorption spectra of AgNCs (line 1), MoS2 (line 2) and MoS2+AgNCs (line 3).

Fig. S5 TEM image of MoS2 nanosheets and AgNCs.

Fig. S6 Absorption spectra of free MoS2 (line 1) and MoS2 in the presence of Na+ (line 2), Na++10 nM ssDNA T1 (line 3), Na++30 nM ssDNA T1 (line 4) and Na+ +50 nM ssDNA T1 (line 5). The concentration of sodium acetate is 50 mM.

Fig. S7 Effects of MoS2 on the fluorescence intensity of AgNCs. The volumes of AgNCs probe (5 μL mL-1) is 25 μL. F' and F'' are the fluorescence intensities of AgNCs before and after addition of MoS2 (1.0 mg mL-1), respectively.

Fig. S8 Effects of salt (sodium acetate) on the fluorescence intensity of AgNC/MoS2 system. The volumes of MoS2 (1.0 mg mL-1) and AgNCs probe (5 μL mL-1) are 200 mL and 25 μL, respectively. F'' and F0 are the fluorescence intensities of AgNC/MoS2 system before and after addition of sodium acetate, respectively.

Fig. S9 Optimization of reaction conditions for detecting DNA based on ssDNA/AgNC/MoS2 system (a, probe concentration; b, pH values). The volume of MoS2 (1.0 mg mL-1) is 200 mL; the concentration of ssDNA is 80 nM; the concentration of sodium acetate is 50 mM; The volume of AgNCs probe (5 μL mL-1) is 25 μL in Fig. S9b.

Fig. S10 Absorption spectra of free MoS2 and MoS2 in the presence of Na+ (line 2), Na++5 nM aptamer (line 3), Na++10 nM aptamer (line 4) and Na+ +20 nM aptamer (line 5). The concentration of sodium acetate is 50 mM.