Course : Fermentation Technology & Natural Product

Course : SY30303

Credit Hour : 3

Lecture : 2 hours/week

Laboratory : 3 hours/week

Lecturers : Assoc. Prof. Dr. Jualang Azlan Gansau and Miss Roslina Jawan

Lecture Hall : BT51 (Bilik Tutorial 51, SST)

Time : Wednesday (10-12pm)

Synopsis

This course aims to complement the knowledge and concepts presented in SY20403 (Biochemical Engineering). In this course the student is introduced to the more important topics about fermentation process and apparatus design. It focuses on the downstream processing aspects of biotechnology and will allow students to be aware of how products and processes are commercialised. The central theme of this course is to provide students with knowledge and understanding the technology, processes, regulatory requirements and commercial issues involved in the production and commercialisation of biotechnology products. Topics to be discussed include microbial growth techniques, isolation, presentation, media characteristics, sterilization, large-scale cell culture, fermentation, downstream processing of products including separation and purification technology, process design, standard operating procedures, compliance and regulatory requirements and the commercialisation considerations in the design of a downstream process are extensively taught. The course also will allow the students to understand the relationship between other unit of operations and fermentation process. Applications of fermentation technology were covered at the end of the syllabus. Natural products topic also has been elaborated which include the classes and application of natural products, isolation and purification, and determination of bioactivity of natural compounds.

Objectives

·  To introduce fermentation process to the students

·  To expose students to the application of fermentation technology in current industries

·  Study the sterilization techniques, microbial section, isolation and preservation

·  Understand the development of inoculum for industrial fermentations

·  Understand fermenter design

·  Understand the technologies use for recovery and purification of fermentation products

·  Know about effluent treatment

Assessment

Quiz : 5%

Practical/Tutorial Report : 15%

Assignment : 10%

Mid Semester Exam : 30%

Final Semester Exam : 40%

Course Content

Week / Scope of Lecture / Remark
1 / An introduction to Fermentation Processes / JAG
2 / Fermentation Pathways / JAG
3 / The Isolation, Preservation and Improvement of Industrial microorganisms / JAG
(Quiz)
4 / Media for Industrial Fermentation; Sterilization; The Development of Inoculum for Industrial Fermentations / JAG
5 /

Design of a Fermenter

/ RJ
6 /

Instrumentation and Control

/ RJ
(Quiz)
7 / Aeration and Agitation / RJ
8 /

MID SEMESTER BREAK

9 / Effluent Treatment ; Fermentation Economics / RJ
10 / Primary and Secondary Metabolism; Introduction to Natural Products Chemistry / RJ
11 / Classes and Functions of Secondary Products / JAG
12 / Screening for Natural Product Activity / JAG
(Quiz)
13 / Modern Methods of Secondary Product Isolation and Analysis / JAG
14 / Large-Scale Production of Secondary Metabolites in Plant Cell Cultures / JAG
15 / Fermentation Applications (liquid and solid-waste management, microbial mass production, primary metabolites production, secondary metabolites production, etc.) / JAG/RJ
(Assignment)

STUDY WEEK

FINAL EXAM

Practical Class

Practical / Practical Topic / Remark
1 / Sauerkraut – A natural tradition fermentation / RJ
2 / Substrate utilization rate of Saccharomyces cerevisae / RJ
3 / Fermentation kinetic study of Saccharomyces cerevisae in batch culture / RJ
4 / Fermentation study of Saccharomyces cerevisae in stirred-tank fermenter / RJ
5 / Natural products isolation, purification and biological activity test / JAG
6 / Field-trip (Industrial visit) / RJ
7 / Field trip (Industrial visit) / RJ

References

1.  Baileys, J.E. and Ollis, D.F. 1986. Biochemical Engineering Fundamentals (2nd). McGraw-Hill Inc. Singapore.

2.  Doble, M. and Gummadi, S.N. 2007. Biochemical Engineering. Prentice-Hall of India Private Limited. New Delhi.

3.  Leland J. Cseke, Ara Kirakosyn, Peter B. Kaufman, Sara L. Warber, James A. Duke and Harry L. Brielmann. 2006. Natural Product from Plants (2nd Edition). CRC Press, Taylor and Francis, USA.

4.  Lixin Zhang and Arnold L. Demain (Eds). 2005. Natural Products: Drug Discovery and Therapeutic Medicine. Humana Press Inc, New Jersey, USA.

5.  Rao, D.G. 2007. Introduction to Biochemical Engineering. Tata McGraw-Hill Publising Company Limited. New Delhi.

6.  Richard J. P. Cannel. 1998. Natural Product Isolation. Humana Press Inc, New Jersey, USA.

7.  Stanbury, P. F. and Whitaker, A. 1984. Principles of Fermentation Technology. Pergamon Press Limited. Oxford.