Supplemental Table 1. The level of GA-intermediates in the swi3c and ga1-3 mutants.

A, Samples collected at the end of the day.

DAY / WT / ga1-3 / swi3c
GA12 / 0,484 (0,037) / 0,120 (0,120) / 0,520 (0,074)
GA15 / 0,450 (0,068) / 2,608 (0,557) / 0,956 (0,019)
GA24 / 2,046 (0,053) / 0,231 (0,048) / 1,898 (0,027)
GA9 / 0,160 (0,006) / 0,017 (0,016) / 0,176 (0,040)
GA4 / 0,396 (0,026) / 0,075 (0,008) / 0,131 (0,019)
GA51 / 0,407 (0,051) / 0,000 (0,000) / 0,586 (0,183)
GA34 / 0,571 (0,008) / 0,012 (0,011) / 0,348 (0,063)
GA53 / 0,108 (0,014) / 0,000 (0,000) / 0,139 (0,003)
GA44 / 0,000 (0,000) / 0,000 (0,000) / 0,010 (0,009)
GA19 / 2,724 (0,393) / 0,000 (0,000) / 4,263 (0,773)
GA20 / 0,000 (0,000) / 0,000 (0,000) / 0,000 (0,000)
GA1 / 0,000 (0,000) / 0,000 (0,000) / 0,000 (0,000)
GA29 / 0,047 (0,024) / 0,137 (0,137) / 0,000 (0,000)

pg/mgFW (SE in brackets)

B, Samples collected at the end of the night.

NIGHT / WT / ga1-3 / swi3c
GA12 / 0,478 (0,038) / 0,000 (0,000) / 0,712 (0,114)
GA15 / 0,256 (0,047) / 1,88 (0,035) / 1,215 (0,275)
GA24 / 2,614 (0,267) / 0,278 (0,042) / 2,501 (0,459)
GA9 / 0,101 (0,013) / 0,000 (0,000) / 0,090 (0,016)
GA4 / 0,291 (0,026) / 0,235 (0,124) / 0,153 (0,048)
GA51 / 0,333 (0,067) / 0,000 (0,000) / 0,578 (0,171)
GA34 / 0,525 (0,044) / 0,000 (0,000) / 0,254 (0,039)
GA53 / 0,159 (0,035) / 0,088 (0,088) / 0,185 (0,002)
GA44 / 0,000 (0,000) / 0,000 (0,000) / 0,000 (0,000)
GA19 / 2,712 (0,046) / 0,000 (0,000) / 4,738 (0,412)
GA20 / 0,000 (0,000) / 0,029 (0,029) / 0,000 (0,000)
GA1 / 0,000 (0,000) / 0,000 (0,000) / 0,000 (0,000)
GA29 / 0,000 (0,000) / 0,060 (0,06) / 0,029 (0,029)

pg/mg FW (SE in brackets)

Supplemental Table 2. Primers used in this work

primer name / sequence / reference
E1F – GA20ox3 / cgatggatacgaagatttcgggaag / qPCR
E1R / tcaaccggaatattgaatcgctgtc / qPCR
E2F – GA3ox2 / ttggctctcccctccacgattt / qPCR
E2R / cccagtgaacctaatgcgaaccac / qPCR
E3F – GA3ox3 / cacaccgactcctccctacttacca / qPCR
E3R / cgcacggtgcattgtacttctgaac / qPCR
SPYL / gaaacccgggtatggtgggactggaagatga / SPY cloning
SPYR / gcagtcgactgctagtggagtccattctctttga / SPY cloning
SMR1-F / gctacgccggctctgatg / (Achard et al., 2009) / qPCR
SMR1-R / cggaggagaagaaacggtca / (Achard et al., 2009) / qPCR
SIM-F / cccacttcttccgaccacaa / (Achard et al., 2009) / qPCR
SIM-R / gccgagagacgacggtgt / (Achard et al., 2009) / qPCR
KRP1-F / gtggaagcaatgaatataagaagaaa / (Achard et al., 2009) / qPCR
KRP1-R / cgacgtttcagtgtcaccat / (Achard et al., 2009) / qPCR
GA3ox1-F / gcttaagtctgctcggtcgg / AtRTPrimer / qPCR
GA3ox1-R / agtgcgatacgagcgacg / AtRTPrimer / qPCR
GA20ox1-F / ggcatcagcgaggagcttatt / AtRTPrimer / qPCR
GA20ox1-R / ctgcttgcgtagccaacact / AtRTPrimer / qPCR
GID1A-F / gatgtcttgattgatcgcaggat / AtRTPrimer / qPCR
GID1A-R / aggaggttgctcttgatctgca / AtRTPrimer / qPCR
GID1B-F / cgagagaccacttcggttatc / AtRTPrimer / qPCR
GID1B-R / ctagtacgcaaacaaagtaccattc / AtRTPrimer / qPCR
GID1C-F / aagcaggaagaagaacagtgtagtc / AtRTPrimer / qPCR
GID1C-R / agtcaaaagctaacgctagaaagc / AtRTPrimer / qPCR
RT-RGA F / cgagagacacgaaacgttga / qPCR
RT-RGA R / agcggaggtggtaatgagtg / qPCR
RGL 1 F / agccaagaccgagtgatgtc / qPCR
RGL 1 R / ggcataaagtgccaacaaca / qPCR
RGL2 F / gacggcgcgtagagttcac / qPCR
RGL2 R / tgcatcccttgattaagccc / qPCR
RGL 3 F / aagcgaaccataacggtgac / qPCR
RGL 3 R / atacgttttcgccactgagc / qPCR
GAI F / gcttatgcaggctcttgcg / qPCR
GAI-R / aaccggaaaacaggaggacc / qPCR
RT-AtCPS/GA1-F / agtttggaggggatgcgtgatttag / AtRTPrimer / qPCR
RT-AtCPS/GA1-R / tgccattgtcggtccaatatctgtg / AtRTPrimer / qPCR
RT-AtKS/GA2-F / cgtggggatgaagaaatatgtttgg / AtRTPrimer / qPCR
RT-AtKS/GA2-R / tgcaaatggttttagcggatcg / AtRTPrimer / qPCR
RT-AtKO/GA3-F / gcccacgaggatacgcaaatcg / AtRTPrimer / qPCR
RT-AtKO/GA3-R / gccattgtcttgtgaagatctgacg / AtRTPrimer / qPCR
Rt-AtKAO1-F / tcgcaggttgtcgatgagacacttc / AtRTPrimer / qPCR
RT-AtKAO1-R / tcagggtcgatgtggacatccctaa / AtRTPrimer / qPCR
RT-AtKAO2-F / caaaaggctggaaggttctgacttg / AtRTPrimer / qPCR
RT-AtKAO2-R / gctcctttccacccggtatttgag / AtRTPrimer / qPCR
bHLH137-F / acattcacgttcgagc / (Zentella et al., 2007) / qPCR
bHLH137-R / cggagccaaagtcatag / (Zentella et al., 2007) / qPCR
Q-WRKY27-F / gcttggcgtaaatacggc / (Zentella et al., 2007) / qPCR
Q-WRKY27-R / ccggcgagagagttcc / (Zentella et al., 2007) / qPCR
Q-SCL3-F / attatgcgatgttgcagg / (Zentella et al., 2007) / qPCR
Q-SCL3-R / attacacccacaccagac / (Zentella et al., 2007) / qPCR
Q-LBD40-F / gtcgagttctacgaaaagg / (Zentella et al., 2007) / qPCR
Q-LBD40-R / ccacaataaccccacg / (Zentella et al., 2007) / qPCR
Q-IQD22-F / gcgatagttagaggcca / (Zentella et al., 2007) / qPCR
Q-IQD22-R / gatgtgtttcctgtcgat / (Zentella et al., 2007) / qPCR
Q-Exp-PT1-F / cttgctctccctgagt / (Zentella et al., 2007) / qPCR
Q-Exp-PT1-R / acacggtgattgtccc / (Zentella et al., 2007) / qPCR
Q-XERICO-F / ccaactctctctacacactt / (Zentella et al., 2007) / qPCR
Q-XERICO-R / gcgaggatgaagacgg / (Zentella et al., 2007) / qPCR
Q-RING-F / gtgcaagaaacaggcagaca / qPCR
Q-RING-R / cctacacaatgggcaagtga / qPCR
Q-bHLH154-F / caagtcaaggttctaagtgctcct / qPCR
Q-bHLH154-R / gcccaaatatcagcaccatt / qPCR
Q-MYB-F / cgagaaacttcgtggtgtca / qPCR
Q-MYB-R / gaacggttgagattggcatt / qPCR
GA2ox3-F / gaccttggctggcttgagtacattc / qPCR
GA2ox3-R / ctcttccttctccgggtaatggttc / qPCR
UBQ / ccagaaggaatcgacgcttcatctc / qPCR
UBQ / gagaaggatcgatctaccgctacaacag / qPCR

AtRTPrimer(Han and Kim, 2006)

Figure S1.The swi3c mutant shows altered responses to exogenously applied hormones: ethylene, ABA and brassinosteroids and an altered gravitropic response.

A,the swi3c mutant plants are hypersensitive to ABA treatment. B,The swi3c mutant exhibits enhanced response to both exogenous and endogenous ethylene demonstrated by shortened and thickened hypocotyl, and exaggerated apical hook.C,swi3c plants are hyposensitive to exogenous brassinosteroids, (D) swi3c plants demonstrate defective gravitropic response. Arrows indicate gravitropic vector (bar 1 cm).

Figure S2. The swi3c mutation confers GA-related growth and developmental defects suppressed by the GA4+7-treatment and insensitivity to the GA biosynthesis inhibitor paclobutrazol (PAC).

Figure S3.Low temperature similarly affects fertility of swi3c and spy-1. GA biosynthesis is altered in swi3c.

A,Silique length and number of seeds produced by wild-type and swi3c plants grown in normal conditions and at decreased temperature. B, The level of GA-intermediates in the swi3c and ga1-3 mutants.

Figure S4. The swi3c mutant exhibits altered expression of genes encoding DELLA represors.

Figure S5.SWI3C interacts with the SPY and DELLA proteins.

A,Negative controls for BiFC analysis of SWI3C-SPY and SWI3C-DELLA interactions. Scale bar 10 μm. B,Yeast two-hybrid assay indicating SWI3C-SPY interaction. Left: negative controls, right: replica lift assayYTH test.

1