Supplementarymaterials
Manuscript title: Dynamic root responses to drought and rewatering in two wheat (Triticumaestivum)genotypes (submitted to Plant Soil)
Sebastian Steinemann1;2, Zhanghui Zeng1,3 Alan McKay4, Sigrid Heuer1, Peter Langridge1, Chun Y Huang1*
1 Australian Centre for Plant Functional Genomics, School of Agriculture, Food and Wine, University of Adelaide, PMB1 Glen Osmond, SA 5064, Australia
2 Plant Breeding, Department of Plant Sciences, TechnischeUniversitätMünchen, Liesel-Beckmann-Str.2, 85354 Freising, Germany
3 State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, 310058, China
4 Plant and Soil Health, South Australian Research and Development Institute, GPO Box 397,
Adelaide, SA 5001, Australia
*Corresponding author:
Chun Y. Huang
Email:
Fig.S1Soil sampling for analyses of root length density and root DNA concentration.
The photo shows a pot after two soil coreswere taken using a soil corer of 2.5 cm in diameter(A). Soil samples were bagged and put on ice for the analysis of root DNA concentration in soil (B).
Fig.S2Rootmorphologyof nodal roots from two contrasting genotypes under moderate drought or well-watered treatments.
Plants were grown in pots and treated either with moderate drought (Drought) or with a well-watered (WW) regime, andharvested at day 50. Roots were washed free of soil, and nodal roots were cut from the root-shoot junction, separated and scanned in a root positioning tray. The three positions of nodal roots used for determination of root morphological parameters in cross sections are indicated in drought-treated RAC875.
Fig. S3 Rootmorphologyof primary roots from two contrasting genotypes under moderate drought or well-watered treatments.
Plants were grown in pots and treated either with moderate drought (Drought) or with a well-watered (WW) regime, andharvested at day 50. Roots were washed free of soil, and primary roots were cut from the root-shoot junction, separated and scanned in a root positioning tray. The two positions of primary roots used for determination of root morphological parameters in cross sections are indicated in WW-treated RAC875.The proximal position was 2 cm from the root-shoot junction, whereas the distal position was 4 cm from the root apex.
Fig.S4Cross sections of nodal roots of RAC875 and Kukri from well-watered and drought-treated plants.
Cross sections of nodal roots 3 cm from the root-shoot junction of well-watered Kukri (A) and RAC875(B) at day 50.Cross sections of nodal roots formoderate drought-treated Kukri (C) and RAC875(D) at day 50. Plants were grown in pots and treated either with moderate drought or with a well-watered regime, andharvested at day 50. Roots were washed free of soil. Nodal roots were hand-cut at 3 cm from the root-shoot junction, stained with phloroglucinol and viewed under a microscope with bright light. Bars indicate 50 µm. Different root tissues are denoted ascortex (CO),endodermis (EN), stele (ST), protoxylem (PX) and metaxylem (MX).
Fig.S5 Cross sections of primary roots(2 cm from the root-shoot junction) in two contrasting genotypes.
Cross sections of primary roots from well-watered Kukri (A) and RAC875(B) at day 50.Cross sections of primary roots from moderate drought-treated Kukri (C) and RAC875(D) at day 50. Plants were grown in pots and treated either with moderate drought or with a well-watered regime, andharvested at day 50. Roots were washed free of soil. Primary roots at 2 cm from the root-shoot junction were sectioned, stained with 0.05% toluidine blue and viewed under a microscope with bright light. Bars indicate 50 µm. Different root tissues are denoted ascortex (CO),endodermis (EN), stele (ST), protoxylem (PX) and metaxylem (MX)
Fig. S6 Cross sections of primary roots(4 cm from the root tip) in two contrasting genotypes.
Cross sections of primary roots from well-watered Kukri (A) and RAC875(B) at day 50.Cross sections of primary roots from moderate drought-treated Kukri (C) and RAC875(D) at day 50. Plants were grown in pots and treated either with moderate drought or with a well-watered regime, andharvested at day 50. Roots were washed free of soil. Primary roots at 4 cm from the root tip were sectioned, stained with 0.05% toluidine blue and viewed under a microscope with bright light. Bars indicate 50 µm.
Fig. S7 Anatomical features of cross sections of nodal roots at 8 cm and 13 cm from the root-shoot junctionin two contrasting genotypes.
(A-D) Cross sections of nodal roots at 8 cm from the root-shoot junction.(E-H)Cross sections of nodal roots at 13 cm from the root-shoot junction. (A, E) Number of metaxylem per root. (B, F) Metaxylem diameter. (C, G) Metaxylem area per root. (D, H) Protoxylem area per root. Plants were grown in pots and treated either with moderate drought or with a well-watered regime, and harvested at day 50. Cross sections were hand-cut at 8 and 13 cm from the root-shoot junction. Each box represents eight sections. Boxes show the median, the 25th and 75th percentiles. Whiskers denote the largest and smallest values. P values werecalculatedwith student’s t-test, and ns is not significant.
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