Microbiology Lab (BIOL 2230) – Fall 2013 PREP SHEETS – LABS 1 THROUGH 3

WK / Topics / Orgs / Media / Media/Equipment
1
8/28 / Syllabus
Lab Procedures & Safety
Microscope (3-1) / none / none / Per Table:
1 bottle immersion oil
1 bottle lens cleaner
2 packets lens paper
Per Student:
1 Leica microscope
On Side Bench:
Prepared slides
·  human blood smear 8
·  yeast 105
·  bacterial types 113
2
9/04 / Microscope Practice
Bacterial Smears & Simple Stains (3-4)
Students will stain one smear with crystal violet and one smear with safranin.
·  S#1: E. coli (2 smears)
·  S#2: S. aureus/epidermidis (2 smears)
·  S#3: B. cereus/subtilis (2 smears)
·  S#4: M. luteus (2 smears)
Negative Stains (3-5) / Per Table: 18 to 24 hour cultures
NA plate:
·  E. coli
·  S. aureus or S. epidermidis
·  B. cereus or B. subtilis
·  M. luteus [48 hr culture]
No INCUBATOR
NA broth:* (4mL)
·  E. coli
·  S. aureus
(*Each student will take one LOOP of each broth culture and mix directly onto slide to be stained.) / For Students:
none
For Class Cultures – W lab:
[4 NAP] x 6 tables
[2 NAB] x 6 tables / Per Table:
1 bottle immersion oil
2 packets lens paper
1 dropper bottle each:
·  deionized water
·  crystal violet
·  Gram’s iodine
·  95% EtOH
·  safranin
2 water spray bottles
1 package bibulous paper
2 Bunsen burners
1 striker
1 Lysol spray bottle
1 package sterile toothpicks
1 beaker w/biohazard bag (used toothpicks)
racks
Per Student:
1 microscope
1 box of slides
1 clothespin (slide holder)
1 staining tray
1 inoculating loop
On Side Bench:
large beaker w/10% bleach (slide disposal)
autoclave tray (culture disposal)
Slide Warmer

Microbiology Lab (BIOL 2230) – SPRING 2009 PREP SHEETS – LABS 1 THROUGH 3

WK / Topics / Orgs / Media / Media/Equipment
3
9/11 / Gram Stain (3-6)
Students will Gram stain one “mixed broth” smear and one gum line smear. / Per Table: 18 to 24 hour cultures
NA slant:
·  E. coli
·  S. aureus
NA broth:
·  E. coli
·  S. aureus
*Each student will take one LOOP of each broth culture and mix directly onto slide to be stained. / Per Students:
none
For Class Cultures
[2 NAS] x 6 tables
[2 NB] x 6 tables / Per Table:
1 bottle immersion oil
2 packets lens paper
1 dropper bottle each:
·  deionized water
·  crystal violet
·  Gram’s iodine
·  95% EtOH
·  safranin
2 water spray bottles
1 package bibulous paper
2 Bunsen burners
1 striker
1 Lysol spray bottle
Per Student:
1 microscope
1 box of slides
1 clothespin (slide holder)
1 staining tray
1 inoculating loop
On Side Bench:
large beaker w/10% bleach (slide disposal)
autoclave tray (culture disposal)
slide warmer