Table S1 PCR Primers Used in This Study

Table S1 PCR primers used in this study

Purpose / Accession number / Forward primer (5´-) / Reverse primer (5´-)
TaTF-YB3;l expression / BT009265 / AGGAGCCATGCCGGAGTCGG / AAGATGTCACCCCTCTTTCCG
Tatubulin expression / U76558 / AGAACACTGTTGTAAGGCTCAAC / GAGCTTTACTGCCTCGAACATGG
TaTF-YB3;l subcellular cassette / BT009265 / TTTGTCGACATGGCGGAGTCGGACA / TTTCCATGGGCCCCTCTTTCCGTCCGA
TaTF-YB3;l overexpression cassette / BT009265 / GAGCCATGGCGGAGTCGGACA / TTGGTAACCAAGATGTCACCCCTCTT
Nttubulin expression / U91563 / TACACAGGGGAAGGAATGG / CTCGAAACCAACGGTATC
NtSOD1 expression / KJ874395 / GTGGACATGTCGTGTCAAGG / TTCTCACCAACTCCTGCACTT
NtSOD2 expression / EU123521 / ATGTCACGGGACCACATTAC / AACCCTTCCACCAGCATTTC
NtFeSOD expression / KF724056 / CATCACAGAGCTTATGTCGACA / CTAGAACTGACTGCTTCCCA
NtMnSOD1 expression / X14482 / TTGGGCTATCGACACTAACTTT / TCAGCCAGCGACTACATGCA
NtMnSOD2 expression / AB093097 / GACGGACCTTAGCAACAGGG / ACCAATGGGTCCTGATTAGCAG
NtCAT expression / EF532799 / CAAGGATCTCTACGACTCGATT / CTTGAGGGCAAATAATCCACCT
NtCAT-1 expression / NTU07627 / GTCTCAGGCTGACAAGTCTT / ACGGAAGACAGAGTAGCAGC
NtCAT1;1 expression / NTU93244 / TCCTGCTAATGCTCCAAAGTGT / AATGCATATGTATTAGGAATGCTC
NtCAT1;2 expression / HF564632 / GGTATCGACTTGGACCAAACTA / GGTCTCACATTAAGCCTAGAAG
NtCAT1;3 expression / HF564631 / TTGCAGCCGGTGGGAAGATT / GGTCTCACATTAAGCCTAGAAG
NtCAT3 expression / HF564633 / GTCTTGGGCCAAACTATCTGCA / TCAGCTTCACATTGTGGGCC
NtPOD1;1 expression / L02124 / GGAATTTGTCCTCAAGGTGGAA / CTTATTGGAATTGCCATTTCAGC
NtPOD1;2 expression / AB044154 / CTGACATGGTCTGTGCCTAC / TCAGTTGATAGCAGAGCAAACTT
NtPOD1;3 expression / AB044153 / AAGATCTTGTCGCTCTTACTGG / AATTGGATTTTCCAGCTTGCG
NtPOD1;4 expression / D11396 / TGCTGGTAGTCAAAGTCAGTTTT / CCCATGTTGAACACGTTCTTACC
NtPOD1;5 expression / AB178953 / AACAGCAACAACGTTAACCCAGC / TTAATTTTGGACCACATTCAGGA
NtPOD1;6 expression / AB027753 / TCAACTCCACTGGTGGCCCT / AATTCGATTTTGCAGCTTGCGC
NtPOD2;1 expression / AB178954 / AGGGGAAAAGACCTCACCAC / AGTTTCCCATCTTGATCATAGCA
NtPOD2;2 expression / KF701483 / AGACAGTGAGTATGCAGCTAA / AAAAAAGCTGCCCTTGGCACC
NtPOD4 expression / AY032675 / AGACTCAAAGATAGCAAACCTCA / CTTCCTGATGTCACCCTTGA
NtPOD9 expression / AY032674 / CACCACCTTCATTCAACGCTA / ACATCTCAGACAAAACACTTGTC
NtPYR1 expression / XM_009778559 / ATGGAGCAATCTGAGAGCTC / CCTTGCTGTATCGTCTTTTCA
NtPYL2 expression / XM_009778517 / TATGGCGGCCTCGGGGTGGT / TTGGTTACCTTCATTCAGCAGG
NtPYL4 expression / XM_009780219 / ATGATGTACCAAGATTTCGGCC / TGTAGTAGACCAGTGTTATCGG
NtPYL8 expression / XM_009788111 / GGACGACAGGTCTGTTTATGG / GAACGTTGTATTCCTTGGCGA
NtYL9 expression / XM_009771158 / GTCGGTGCCGTTAGTTTTTG / AGACACCCGCATATGCAGA
NtPYL11 expression / XM_009596711 / GCCGAAGCAACGAGAGAC / ATCTAACAAGGTATTCGTCATG
NtPYL4 overexpression cassete / XM_009780219 / AAACCATGGTTCCCAATAGTCAG / GGTAACCGGATTTGCGCTGGAA
NtPYL4 antisense expression cassette / XM_009780219 / TTTCCATGGTAATTATTGTCCTTT / TTTGGTGACCCAACCAGTGTTGC
NtPIN1 expression / KC347302 / ATGATGTACCAAGATTTCGGCC / TGTAGTAGACCAGTGTTATCGG
NtPIN3 expression / KC425459 / GGTTGTAGTTTCGGCCCCGT / ACCAGGAGATTGGTACTTGATC
NtPIN4 expression / KC433529 / AATCCGACTCTGATCCGGTTA / CTATACACTAGTGCATGCGCA
NtPIN9 expression / KC433528 / TCTGATGCTGGCCTTGGAATG / AGTGATAGGAAGAGCTACCAAC
NtPIN1b expression / KC460399 / GGACGACAGGTCTGTTTATGG / GAACGTTGTATTCCTTGGCGA
NtPIN3b expression / KC438370 / CCCTTGATGCCTCTGTAGTTTC / ACGCAGTGCTAAGAATAGCCGG
NtPIN4 sense expression cassette / KC433529 / TTTCCATGGTCACTTGGCACGAT / TTTGGTAACCTTATAATCCAAGAATG
NtPIN4 antisense expression cassette / KC433529 / TTTCCATGGCAATGCTATCAACAT / TTTGGTAACCAAAATCATAGAGAAA

Fig. S1Biomass of Shixin 828 and other nine wheat cultivars under normal growth condition and simulated drought stress

The biomass of wheat cultivars were derived from plants cultured hydroponically in MS solution (normal condition) and modified MS solution (supplemented with 5% PEG-6000) for three weeks. Data are shown means ±SE from four replicates in each three representative plants used. Asterisk stands for to significant compared with the control cultivar Shiluan 02-1 (Student’s t test, P<0.05).

Fig. S2 Alignment between TaNF-YB3;landthe TaNF-YB3;l-EST

Fig. S3 Green fluorescent protein (GFP) signal localization of TaNF-YB3;l-GFP fusion in transgenic tobacco epidermal cells

1. The fusion detected under green light. b. The fusion detected under white light. Arrows point to nucleus.

Fig. S4Phylogenetic relations between TaNF-YB3;l and its homologous partners across various plant species

Fig. S5 Relative expression of TaNF-YB3;l in overexpression lines

WT, wild type. Line 1 to Line 9, lines with overexpression of TaNF-YB3;l. Data are shown means ±SE from four replicates in each three representative plants used.

Fig. S6 Expression patterns of three differential POD encoding genes in TaNF-YB3;l overexpression lines under normal condition and drought stress

WT, wild type. Line 2, a tobacco transgenic line with overexpression of TaNF-YB3;l. NtPOD1;3, NtPOD1;5, and NtPOD2;1, three tobacco POD encoding genes. Data are shown means ±SE from four replicates in each three representative plants used. Asterisk stands for to significant compared with WT (Student’s t test, P<0.05).

Fig. S7 Expression patterns of NtPYL4 in TaNF-YB3;l overexpression line under normal condition, drought stress, and exogenous ABA treatment

WT, wild type. Line 2, a tobacco transgenic line with overexpression of TaNF-YB3;l. Data are shown means ±SE from four replicates in each three representative plants used. Asterisk stands for to significant compared with WT (Student’s t test, P<0.05).

Fig. S8 Relative expression of TaPYL4 in transgenic lines

WT, wild type. S1 to S7, lines with overexpression of TaPYL4. A1 to A6, lines with knockdown expression of TaPYL4. Data are shown means ±SE from four replicates in each three representative plants used.

Fig. S9 Expression patterns of NtPIN4 in TaNF-YB3;l overexpression line under normal condition drought stress

WT, wild type. Line 2, a tobacco transgenic line with overexpression of TaNF-YB3;l. Data are shown means ±SE from four replicates in each three representative plants used. Asterisk stands for to significant compared with WT (Student’s t test, P<0.05).

Fig. S10 Relative expression of TaPIN4 in transgenic lines

WT, wild type. O1 to O6, lines with overexpression of TaPIN4. K1 to K7, lines with knockdown expression of TaPIN4. Data are shown means ±SE from four replicates in each three representative plants used.