Locality Information, Genbank Accession Numbers (GB Acc. #), and Museum Numbers of All

Table 1

Locality information, GenBank accession numbers (GB Acc. #), and museum numbers of all analysed specimens. Individuals sequenced in the course of this study hold an individual four-digit DNA collection number of the University Giessen (column “Spm #”) whereas “Gb” indicates that sequences were taken from GenBank. Haplotype numbers (column “Hpt #”) refer to the unique haplotypes in figure 4. When species determination was ambiguous (e.g. for juveniles), we assigned a cf. (con forma) to the species name. Individuals originally determined as L. ellipticus or L. ovum from Lake Malawi are referred to as Lanistes sp. (ellipticus-like) and Lanistes sp. (ovum-like). (*) Shell was destroyed during DNA isolation; tissue and shell fragments are stored at Giessen University (UGSB collection).

Species / Hpt # / Spm # / Country / Locality / Latitude / Longitude / COI GB Acc. # / 16S GB Acc # / Museum voucher #
Pila conica / Gb / Vietnam / Hanoi / EU274570 / EU274495
P. ovata / Gb / Uganda / Mpunge / EU274571 / EU274498
L. carinatus / Gb / Uganda / L. Bisinia / EU2744560 / EU274486
L. ellipticus / 9740 / Mozambique / Mz-01 / -16.15492 / 33.59914 / FJ831731 / ZMB115000
Lanistes sp. (ellipticus-like) / hpt17 / 6590 / Malawi / Ma-05 / -9.90481 / 33.94697 / FJ831703 / BMNH20090138
6579 / Malawi / Ma-10 / -9.97632 / 33.93944 / FJ831711 / BMNH20090139
hpt09 / 9744 / Malawi / Ma-18 / -12.92111 / 34.21077 / FJ831738 / BMNH20090140
9745 / Malawi / Ma-18 / -12.92111 / 34.21077 / FJ831741 / BMNH20090141
9746 / Malawi / Ma-18 / -12.92111 / 34.21077 / FJ831740 / BMNH20090142
6175 / Malawi / Ma-12 / -10.15455 / 34.04110 / FJ831702 / BMNH20090143
hpt07 / Gb / Tanzania / Ta-03 / EU2744561 / EU274487
L. nasutus / hpt13 / 7412 / Malawi / Ma-22 / -14.04809 / 34.92171 / FJ831725 / FJ831778 / *
L. nyassanus / hpt10 / 6573 / Malawi / Ma-21 / -14.01204 / 34.85013 / FJ831692 / FJ831769 / BMNH20090144
hpt04 / 6574 / Malawi / Ma-21 / -14.01204 / 34.85013 / FJ831691 / BMNH20090145
hpt18 / 7040 / Malawi / Ma-25 / -14.08923 / 34.93017 / FJ831688 / FJ831768 / BMNH20090146
hpt16 / 7055 / Malawi / Ma-25 / -14.08923 / 34.93017 / FJ831686 / FJ831766 / BMNH20090147
hpt11 / 6586 / Malawi / Ma-20 / -14.01498 / 34.84807 / FJ831696 / FJ831773 / BMNH20090148
hpt12 / 6587 / Malawi / Ma-20 / -14.01498 / 34.84807 / FJ831695 / FJ831772 / BMNH20090149
hpt15 / 7038 / Malawi / Ma-16 / -12.88355 / 34.29794 / FJ831715 / FJ831775 / BMNH20090150
hpt01 / 5554 / Malawi / Ma-11 / -10.00121 / 33.93676 / FJ831760 / BMNH20090151
5555 / Malawi / Ma-11 / -10.00121 / 33.93676 / FJ831761 / BMNH20090152
5558 / Malawi / Ma-09 / -9.94616 / 33.94450 / FJ831758 / FJ831793 / BMNH20090153
hpt20 / 7408 / Malawi / Ma-15 / -12.07993 / 34.71584 / FJ831727 / FJ831780 / BMNH20090154
hpt21 / 7409 / Malawi / Ma-15 / -12.07993 / 34.71584 / FJ831726 / FJ831779 / BMNH20090155
hpt14 / Gb / Malawi / Msaka / EU2744562 / EU274488
L. cf. nyassanus / hpt02 / 6581 / Malawi / Ma-13 / -10.21013 / 34.09455 / FJ831701 / FJ831774 / BMNH20090156
L. ovum / hpt34 / 9734 / Mozambique / Mz-01 / -16.15492 / 33.59914 / FJ831737 / ZMB115001
9735 / Mozambique / Mz-01 / -16.15492 / 33.59914 / FJ831736 / ZMB115001
9736 / Mozambique / Mz-01 / -16.15492 / 33.59914 / FJ831735 / ZMB115001
9737 / Mozambique / Mz-01 / -16.15492 / 33.59914 / FJ831734 / ZMB115001
9738 / Mozambique / Mz-01 / -16.15492 / 33.59914 / FJ831733 / ZMB115001
9739 / Mozambique / Mz-01 / -16.15492 / 33.59914 / FJ831732 / ZMB115001
hpt35 / 9741 / Mozambique / Mz-01 / -16.15492 / 33.59914 / FJ831730 / ZMB115001
L. ovum-complex / hpt25 / 7335 / Malawi / Ka-01 / -11.14105 / 33.61758 / FJ831754 / FJ831789 / BMNH20090164
hpt24 / 7334 / Malawi / Ka-01 / -11.14105 / 33.61758 / FJ831753 / BMNH20090165
7333 / Malawi / Ka-01 / -11.14105 / 33.61758 / FJ831752 / FJ831788 / BMNH20090166
hpt26 / 7514 / Namibia / Na-02 / -17.98192 / 23.33815 / FJ831744 / FJ831783 / ZMB115002
hpt28 / 7515 / Namibia / Na-02 / -17.98192 / 23.33815 / FJ831745 / FJ831784 / ZMB115002
hpt27 / 7516 / Namibia / Na-01 / -17,5 / 24,26666 / FJ831742 / FJ831781 / ZMB115003
hpt29 / 7518 / Botswana / Bo-01 / -19,98333 / 23,41666 / FJ831746 / FJ831785 / ZMB115004
7519 / Botswana / Bo-01 / -19,98333 / 23,41666 / FJ831747 / ZMB115004
hpt30 / 7520 / Botswana / Bo-01 / -19,98333 / 23,41666 / FJ831748 / FJ831786 / ZMB115004
hpt29 / 7521 / Botswana / Bo-01 / -19,98333 / 23,41666 / FJ831749 / ZMB115004
7522 / Botswana / Bo-01 / -19,98333 / 23,41666 / FJ831750 / ZMB115004
hpt31 / 7523 / Botswana / Bo-01 / -19,98333 / 23,41666 / FJ831751 / FJ831787 / ZMB115004
hpt32 / 7517 / Namibia / Na-01 / -17,5 / 24,26666 / FJ831743 / FJ831782 / ZMB115003
hpt22 / 7052 / Tanzania / Ta-02 / -5.25140 / 31.08518 / FJ831757 / FJ831792 / BMNH20090177
hpt23 / 7030 / Tanzania / Ta-02 / -5.25140 / 31.08518 / FJ831756 / FJ831791 / BMNH20090178
hpt33 / Gb / Zambia / Za-01 / EU2744564 / /
Lanistes sp. (ovum-like) / hpt17 / 6592 / Malawi / Ma-05 / -9.90481 / 33.94697 / FJ831705 / BMNH20090179
hpt04 / 6593 / Malawi / Ma-05 / -9.90481 / 33.94697 / FJ831704 / BMNH20090180
hpt17 / 6583 / Malawi / Ma-06 / -9.90137 / 33.94536 / FJ831706 / BMNH20090181
6580 / Malawi / Ma-04 / -9.89932 / 33.94370 / FJ831707 / BMNH20090182
hpt04 / 7405 / Malawi / Ma-04 / -9.89932 / 33.94370 / FJ831728 / BMNH20090183
hpt17 / 6172 / Malawi / Ma-07 / -9.93742 / 33.94602 / FJ831698 / BMNH20090184
hpt06 / 7036 / Malawi / Ma-01 / -9.67709 / 33.85261 / FJ831713 / FJ831775 / BMNH20090185
hpt07 / 7037 / Malawi / Ma-01 / -9.67709 / 33.85261 / FJ831712 / BMNH20090186
hpt17 / 7329 / Malawi / Ma-03 / -9.89137 / 33.92692 / FJ831708 / BMNH20090187
hpt07 / 7421 / Malawi / Ma-30 / -15.38884 / 34.90498 / FJ831716 / BMNH20090188
hpt08 / 7419 / Malawi / Ma-30 / -15.38884 / 34.90498 / FJ831717 / FJ831777 / BMNH20090189
hpt07 / 7415 / Malawi / Ma-31 / -15.44576 / 34.86306 / FJ831718 / BMNH20090190
7404 / Malawi / Ma-17 / -12.92552 / 34.29539 / FJ831729 / BMNH20090191
7400 / Malawi / Ma-29 / -15.05699 / 35.22272 / FJ831721 / BMNH20090192
7417 / Malawi / Ma-28 / -14.71752 / 35.17714 / FJ831723 / BMNH20090193
7418 / Malawi / Ma-28 / -14.71752 / 35.17714 / FJ831722 / BMNH20090194
7398 / Malawi / Ma-27 / -14.45179 / 35.24633 / FJ831724 / BMNH20090195
hpt04 / 6585 / Malawi / Ma-14 / -10.42559 / 34.25012 / FJ831699 / BMNH20090196
6582 / Malawi / Ma-13 / -10.21013 / 34.09455 / FJ831700 / BMNH20090197
hpt17 / 7317 / Malawi / Ma-08 / -9.94581 / 33.94455 / FJ831709 / BMNH20090198
7316 / Malawi / Ma-08 / -9.94581 / 33.94455 / FJ831710 / BMNH20090199
7314 / Malawi / Ma-02 / -9.75928 / 33.88786 / FJ831714 / BMNH20090200
hpt07 / 7401 / Malawi / Ma-29 / -15.05699 / 35.22272 / FJ831720 / BMNH20090201
7402 / Malawi / Ma-29 / -15.05699 / 35.22272 / FJ831719 / BMNH20090202
hpt09 / 9743 / Malawi / Ma-18 / -12.92111 / 34.21077 / FJ831739 / BMNH20090203
L. purpureus / Gb / Zanzibar / Zb-01 / EU2744565 / EU274490
L. solidus / hpt04 / 6575 / Malawi / Ma-21 / -14.01204 / 34.85013 / FJ831690 / BMNH20090204
6576 / Malawi / Ma-21 / -14.01204 / 34.85013 / FJ831689 / BMNH20090205
hpt19 / 6577 / Malawi / Ma-21 / -14.01204 / 34.85013 / FJ831693 / FJ831770 / BMNH20090206
hpt03 / 6168 / Malawi / Ma-26 / -13.98641 / 34.51880 / FJ831683 / FJ831764 / BMNH20090207
hpt04 / 6169 / Malawi / Ma-26 / -13.98641 / 34.51880 / FJ831682 / FJ831763 / BMNH20090208
hpt17 / 5556 / Malawi / Ma-11 / -10.00121 / 33.93676 / FJ831762 / BMNH20090209
5559 / Malawi / Ma-09 / -9.94616 / 33.94450 / FJ831759 / BMNH20090210
hpt07 / 6571 / Malawi / Ma-24 / -14.07031 / 34.91693 / FJ831684 / FJ831765 / BMNH20090211
6572 / Malawi / Ma-24 / -14.07031 / 34.91693 / FJ831685 / BMNH20090212
7043 / Malawi / Ma-19 / -14.25598 / 34.80871 / FJ831697 / BMNH20090213
Gb / Malawi / Chipanda / EU2744566 / EU274491
L. cf. solidus / hpt05 / 7041 / Malawi / Ma-25 / -14.08923 / 34.93017 / FJ831687 / FJ831767 / BMNH20090214
hpt17 / 6589 / Malawi / Ma-20 / -14.01498 / 34.84807 / FJ831694 / FJ831771 / BMNH20090215
L. cf. stuhlmanni / 7053 / Tanzania / Ta-01 / -5.11781 / 30.37430 / FJ831755 / FJ831790 / BMNH20090216
L. varicus / Gb / Burkina Faso / EU2744567 / EU274492