DNA template

Sequencing primer

Ready Reaction Mix

2.5xsequencing buffer,ieHazardous Substances Policy - Assessment

CHEMICAL HAZARD AND RISK ASSESSMENT

School of Biosciences

Name of supervisor / Dr S. Minchin / Assessment Number* / 1794
Signature
Assessor / Rita Godfrey / Date of Assessment / 11/4/01

Signature

NotesA School COSHH form in Word is available on the School Server.

Available from the Health and Safety Unit.

Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment.

Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form.

Use a continuation sheet to expand any section of this form in hard copy version.

1 / LOCATION OF THE WORK ACTIVITY / Lab G10 and Genomics Lab
2 / PERSONS WHO MAY BE AT RISK
List names where possible / All occupants of lab G10 (Jo Field, Dong Ling Zeng, Rita Godfrey, Laura Tate)and
the Genomics lab.
3 / ACTIVITY ASSESSED / Big Dye Terminator sequencing
4 / MATERIALS INVOLVED / Attach copies of data sheet(s)
NAME / AMOUNT per sample* / HAZARD / RISK PHRASES / HAZDAT NO*** / BIOSCIENCESNO***
DNA template / 200-500ng / NSH under
GMAG rules
Sequencing primer / 3.2pmol / NSH
Ready Reaction Mix / 4ul / NSH
2.5xsequencing buffer,ie / 4ul / NSH
200mM Tris-HCl [pH 9.0], 5mM MgCl2
Sterile Water / up to 20ul / NSH
75% Isopropanol / 80ul / FLAMMABLE / R11- Highly Flammable / 127
IRRITANT / R36-Irritating to the eyes
R67-Vapours may cause drowsiness and dizziness
70% Ethanol / 250ul / FLAMMABLE / R11- Highly Flammable / 84
Hi-Di Formamide [99.5% formamide, 0.11% EDTA, 0.39% water] / 10ul / TOXIC
IRRITANT / R61-May cause harm to the unborn child.Category
2 risk to reproduction
R36/37/38-Irritating to eyes, respiratory system and skin. / 261
5 / INTENDED USE
Give brief details and attach protocol/instructions / Sequencing plasmid DNA samples using the Big Dye Terminator
kit and the 3700 DNA Analyser in the Genomics lab
6 / RISKS to HEALTH and SAFETY from INTENDED USE
From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding mothers especially at risk?
There is a fire risk from using 75% Isopropanol and 70% Ethanol, and a possible risk of irritation to the
eyes from the isopropanol. Large quantities of isopropanol used on the open bench may cause drowsiness
or dizziness. The Hi-Di formamide solution poses a risk to pregnant women especially, and there is also a
risk to eyes, skin and the respiratory system from it.
7 / CONCLUSIONS ABOUT RISKS
Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary?
The fire risk needs to be controlled. The risk from the Hi-Di Formamide is very slight due to the very small
volumes used, although the risk increases when aliquoting from the main stock into tubes.
8 / CONTROL MEASURES
Additional to Good Chemical Practice
All sources of ignition should be removed when working with isopropanol or ethanol. Large volumes of
isopropanol should not be left out on the open bench next to the worker for more than a few minutes.
Tops should be replaced on bottles and tubes as soon as possible. Open bottles and tubes containing
Hi-Di Formamide should not be used in large quantities in the viscinity of pregnant women. Pregnant women themselves who have to use this ought to handle it in a fume hood if there are a large number of
samples. The fume hood should be checked before use to make sure that it is working properly.
9 / INSTRUCTION/TRAINING
Specify course(s) and/or special arrangements.
10 / MONITORING
Performance of control measures,
Personal exposure / Health Surveillance
11 / WASTE DISPOSAL PROCEDURE
See School Server for Approved Procedure Document on specific Chemical Waste Disposal.
All waste isopropanol and ethanol should be collected in a waste non-halogenated solvent bottle which
when full should be taken down to the Stores for specialist disposal. All of the sequencing sample is
loaded onto the 3700 DNA Analyser and so is not returned to the user for disposal. Should there be any
Hi-Di formamide to dispose of, it should also be placed in the Waste Non-Halogenated Solvent bottle.
12 / REVIEW
Enter the date or circumstances for review of assessment (maximum review interval 5 years)
April 2006
13 / EMERGENCY ACTION
TO CONTROL HAZARDS / To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc.
Isopropanol / Ethanol - Shut off all sources of ignition. Soak up small spills with the lab spill kit. Prevent material from entering drains, and guard against static electricity. Transfer solvent to a suitable container.
Hi-Di Formamide – soak up with lab spill kit
TO PROTECT PERSONNEL / Evacuation, protection for personnel involved in clean-up, Special First Aid
Personnel not involved in the clean up should stay out of the area. The affected area should be ventilated
in cases involving all the chemicals used in this assessment.
Ethanol or isopropanol – avoid build up of static electricity.
TO RENDER SITE OF EMERGENCY SAFE / Clean-up/decontamination
Thoroughly wash the contaminated area with lots of water in all cases.
CONTACT PHONE 0121 414 5438

* Prefix T is used for Teaching Assessment Number.

** List the amount by weight of the substance used. (for liquids eg; 100 mls 1M Sodium Hydroxide =

4g).

***Hazdat No is the UNICOSHH datasheet report number.

Biosciences No is the Biosciences data sheet number.

UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER.

BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.