Supplemental Table S1. Bacterial strains, plasmids and oligonucleotides used in this study

Bacterial strains / Phenotype, genotype, description, or sequence a / Source
P. aeruginosa
PAO1 / Alg- Prototroph / P. Phibbs
PAO1kinB::aacC1 / Alg+, PAO1 kinB:: Gmr / (1)
PAO1kinB::aacC1ΔalgU / Alg- , PAO1kinB::aacC1 in-frame deletion of algU (PA0762) / (1)
PAO1kinB::aacC1ΔrpoN / Alg- , PAO1kinB::aacC1 in-frame deletion of rpoN (PA4462) / (1)
PAO1ΔrhlA / Alg-, PAO1 with rhlA deleted / (3)
PDO300 / Alg+, PAO1 mucA22 / (2)
E. coli
DH5α / F-, φ80dlacZΔM15, Δ(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(rk-, mk+), phoA, supE44, λ-, thi-1, gyrA96, relA1 / Lab strain
TOP10 / F– mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara leu) 7697 galU galK rpsL (StrR) endA1 nupG / Invitrogen
Oligonucleotide
pchA F / CTACCGGGTACTCTGCCAAC
pchA R / CTCACCTTGGCTTCCCATT
pchG F / CTCGTGCTCAACGAACTGG
pchG R / GAACAGGCCGTCGTCATC
fptA F / TCGTGGTATGTCAGCTACGC
fptA R / ATTGTTCTCCAGGTCGATGC
algW F / ACACCACCAAGATGGTCAGC
algW R / GTCACGTGGTTGTTGGTCAG
PA1471 F / CTGGCGAGCTTTCTCCTGT
PA1471 R / CAAGCCCTTGCTGTTGTGAT
lecA F / TATCTACAATCCGGGCGATG
lecA R / TTGCCAATCTTCATGACCAG
treA F / CCCTGCTCTACCATCTCGAA
treA R / CCAGTCGTAGTCGGCGTAGT
katE F / AGGGTCGGCTGTTCTCCTAT
katE R / GTCGATCGAGTTCGGTTCAT
PA2146 F / AGGTGGTAAAGGCAACTTCG
PA2146 R / CGCTGGCCACCTTTCTTG
mucA F / CTGGTCGCGTTACCAGTTG
mucA R / GGTCCCTTCTCCGCTTTC
algU F / GCGGCGATAGTGCTTTTTAT
algU R / GAAGAACTCCGCATCCTCTG
aprA F / CTTCAATACGCCGTGGAAGT
aprA R / GCGTCGACGAAGTGGATATT
aprD F / CAACGCGCTGAGCAAATAC
aprD R / CCGATCAGTTGGTCGATAGG
rhlB F / GAGCGACGAACTGACCTACC
rhlB R / GGGAATCCCGTACTTCTCGT
rhlA F / CGAGGTCAATCACCTGGTCT
rhlA R / GACGGTCTCGTTGAGCAGAT
rhlI F / AAGGACGTCTTCGCCTACCT
rhlI R / GCAGGCTGGACCAGAATATC
lasB F / ACATCGCCCAACTGGTCTAC
lasB R / ACTTGCCGATCTTCTGGTTG
lasI F / CGTGCTCAAGTGTTCAAGGA
lasI R / AAAACCTGGGCTTCAGGAGT
omlA F / AAAATCGACATCCAGCAAGG
omlA R / GGTCGCTGTCGTTGAAGAAC

a Alg-, non-mucoid phenotype and Alg+, mucoid phenotype

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2. Mathee, K., O. Ciofu, C. Sternberg, P. W. Lindum, J. I. Campbell, P. Jensen, A. H. Johnsen, M. Givskov, D. E. Ohman, S. Molin, N. Hoiby, and A. Kharazmi. 1999. Mucoid conversion of Pseudomonas aeruginosa by hydrogen peroxide: a mechanism for virulence activation in the cystic fibrosis lung. Microbiology 145:1349-57.

3. Rahim, R., U. A. Ochsner, C. Olvera, M. Graninger, P. Messner, J. S. Lam, and G. Soberon-Chavez. 2001. Cloning and functional characterization of the Pseudomonas aeruginosa rhlC gene that encodes rhamnosyltransferase 2, an enzyme responsible for di-rhamnolipid biosynthesis. Mol Microbiol 40:708-18.