Supplemental Table 1.
All MS2 spectra from 18O-labeled rat skeletal muscle protein homogenate that matched to database within 8ppm mass accuracy
MS2 spectra matched to HNE-modified peptides (210)Protein name / Scan number / 18O labeled? / Precursor charge / Peptide sequence / SEQUEST XCorr score / SEQUEST DCn score / Number of enzymatic termini / Variable modifications / Delta mass (ppm)
actin, alpha 1, skeletal muscle / 7455 / y / 2 / hQGVMVGMGQK / 2.79 / 0.282 / 2 / h1: # (+160.13) / -0.1486
actin, alpha 1, skeletal muscle / 7505 / y / 2 / hQGVMVGMGQK / 3.61 / 0.272 / 2 / h1: # (+160.13) / -0.2237
actin, alpha 1, skeletal muscle / 3962 / y / 2 / hQGVmVGm / 2.14 / 0.112 / 1 / h1: # (+160.13), m5: Oxidation (+15.99), m8: Oxidation (+15.99) / -1.645
actin, alpha 1, skeletal muscle / 4055 / y / 2 / hQGVmVGm / 2.44 / 0.166 / 1 / h1: # (+160.13), m5: Oxidation (+15.99), m8: Oxidation (+15.99) / -1.835
actin, alpha 1, skeletal muscle / 4175 / y / 2 / hQGVmVGm / 2.45 / 0.088 / 1 / h1: # (+160.13), m5: Oxidation (+15.99), m8: Oxidation (+15.99) / -2.311
actin, alpha 1, skeletal muscle / 7554 / n / 2 / hQGVMVGMGQK / 3.46 / 0.332 / 2 / h1: # (+160.13) / -1.05
actin, alpha 1, skeletal muscle / 6835 / n / 2 / hQGVmVGMGQK / 3.01 / 0.234 / 2 / h1: # (+160.13), m5: Oxidation (+15.99) / -0.9
actin, alpha 1, skeletal muscle / 6878 / n / 2 / hQGVmVGMGQK / 2.16 / 0.11 / 2 / h1: # (+160.13), m5: Oxidation (+15.99) / -1.716
actin, alpha 1, skeletal muscle / 6924 / n / 3 / hQGVmVGMGQK / 2.22 / 0.125 / 2 / h1: # (+160.13), m5: Oxidation (+15.99) / -1.345
actin, alpha 1, skeletal muscle / 6925 / n / 2 / hQGVmVGMGQK / 2.84 / 0.228 / 2 / h1: # (+160.13), m5: Oxidation (+15.99) / -1.716
actin, alpha 1, skeletal muscle / 7021 / n / 2 / hQGVmVGMGQK / 2.61 / 0.27 / 2 / h1: # (+160.13), m5: Oxidation (+15.99) / -0.529
actin, alpha 1, skeletal muscle / 3729 / n / 2 / hQGVmVGmGQK / 2.28 / 0.167 / 2 / h1: # (+160.13), m5: Oxidation (+15.99), m8: Oxidation (+15.99) / -0.4605
actin, alpha 1, skeletal muscle
Continued / 3780 / n / 2 / hQGVmVGmGQK / 2.58 / 0.204 / 2 / h1: # (+160.13), m5: Oxidation (+15.99), m8: Oxidation (+15.99) / -1.414
Protein name / Scan number / 18O labeled? / Precursor charge / Peptide sequence / SEQUEST XCorr score / SEQUEST DCn score / Number of enzymatic termini / Variable modifications / Delta mass (ppm)
actin, alpha 1, skeletal muscle / 3787 / n / 3 / hQGVmVGmGQK / 2.32 / 0.0815 / 2 / h1: # (+160.13), m5: Oxidation (+15.99), m8: Oxidation (+15.99) / -0.6072
actin, alpha 1, skeletal muscle / 3829 / n / 2 / hQGVmVGmGQK / 1.98 / 0.154 / 2 / h1: # (+160.13), m5: Oxidation (+15.99), m8: Oxidation (+15.99) / -0.6072
actin, alpha 1, skeletal muscle / 4008 / n / 2 / hQGVmVGm / 2.46 / 0.187 / 1 / h1: # (+160.13), m5: Oxidation (+15.99), m8: Oxidation (+15.99) / -2.502
actin, alpha 1, skeletal muscle / 4414 / n / 2 / hQGVmVGm / 2.04 / 0.0836 / 1 / h1: # (+160.13), m5: Oxidation (+15.99), m8: Oxidation (+15.99) / -0.217
actin, alpha 1, skeletal muscle / 5990 / n / 2 / hQGVmVGm / 2.18 / 0.131 / 1 / h1: # (+160.13), m5: Oxidation (+15.99), m8: Oxidation (+15.99) / -1.359
actin, alpha 1, skeletal muscle / 6133 / n / 2 / hQGVmVGmGQK / 2.09 / 0.093 / 2 / h1: # (+160.13), m5: Oxidation (+15.99), m8: Oxidation (+15.99) / -1.194
actin, alpha 1, skeletal muscle / 6220 / n / 2 / hQGVmVGmGQK / 2.16 / 0.096 / 2 / h1: # (+160.13), m5: Oxidation (+15.99), m8: Oxidation (+15.99) / -0.02051
actin, alpha 1, skeletal muscle / 6272 / n / 2 / hQGVmVGmGQK / 2.44 / 0.188 / 2 / h1: # (+160.13), m5: Oxidation (+15.99), m8: Oxidation (+15.99) / -0.02051
actin, alpha 1, skeletal muscle / 6294 / n / 3 / hQGVmVGmGQK / 4.16 / 0.199 / 2 / h1: # (+160.13), m5: Oxidation (+15.99), m8: Oxidation (+15.99) / -0.3872
actin, alpha 1, skeletal muscle / 6365 / n / 2 / hQGVmVGmGQK / 2.84 / 0.3 / 2 / h1: # (+160.13), m5: Oxidation (+15.99), m8: Oxidation (+15.99) / -0.8271
actin, alpha 1, skeletal muscle / 6379 / n / 3 / hQGVmVGmGQK / 3.54 / 0.209 / 2 / h1: # (+160.13), m5: Oxidation (+15.99), m8: Oxidation (+15.99) / -0.8271
actin, alpha 1, skeletal muscle / 6409 / n / 2 / hQGVmVGmGQK / 2.2 / 0.0377 / 2 / h1: # (+160.13), m5: Oxidation (+15.99), m8: Oxidation (+15.99) / -0.9005
actin, alpha 1, skeletal muscle / 6420 / n / 3 / hQGVmVGmGQK / 2.96 / 0.0555 / 2 / h1: # (+160.13), m5: Oxidation (+15.99), m8: Oxidation (+15.99) / -0.6072
actin, alpha 1, skeletal muscle / 6893 / n / 2 / hQGVmVGmGQK / 2.38 / 0.171 / 2 / h1: # (+160.13), m5: Oxidation (+15.99), m8: Oxidation (+15.99) / -0.6805
actin, alpha 1, skeletal muscle / 6945 / n / 2 / hQGVmVGmGQK / 2.51 / 0.205 / 2 / h1: # (+160.13), m5: Oxidation (+15.99), m8: Oxidation (+15.99) / -1.634
Continued
Protein name / Scan number / 18O labeled? / Precursor charge / Peptide sequence / SEQUEST XCorr score / SEQUEST DCn score / Number of enzymatic termini / Variable modifications / Delta mass (ppm)
actin, alpha 1, skeletal muscle / 3780 / n / 2 / hQGVmVGmGQK / 2.58 / 0.204 / 2 / h1: # (+160.13), m5: Oxidation (+15.99), m8: Oxidation (+15.99) / -1.414
actin, alpha 1, skeletal muscle / 6997 / n / 2 / hQGVmVGmGQK / 2.07 / 0.0914 / 2 / h1: # (+160.13), m5: Oxidation (+15.99), m8: Oxidation (+15.99) / -1.267
actin, alpha 1, skeletal muscle / 4463 / n / 2 / hQGVMVGm / 1.66 / 0.121 / 1 / h1: # (+160.13), m8: Oxidation (+15.99) / -1.462
actin, alpha 1, skeletal muscle / 6972 / n / 2 / hQGVMVGmGQK / 2.84 / 0.2 / 2 / h1: # (+160.13), m8: Oxidation (+15.99) / -1.123
actin, alpha 1, skeletal muscle / 7793 / n / 2 / VAPEEhPTLLTEAPLNPK / 4.51 / 0.403 / 2 / h6: # (+160.13) / -2.504
actin, alpha 1, skeletal muscle / 7836 / n / 3 / VAPEEhPTLLTEAPLNPK / 2.83 / 0.362 / 2 / h6: # (+160.13) / 1.182
actinin alpha 3 / 7809 / n / 2 / SVEETQSLLTAhEQFK / 2.01 / 0.225 / 1 / h12: # (+160.13) / -0.846
aldolase A / 7352 / n / 3 / PhPYPALTPEQK / 2.92 / 0.167 / 1 / h2: # (+160.13) / -0.1287
aldolase A / 7404 / n / 3 / PhPYPALTPEQK / 3.49 / 0.0694 / 1 / h2: # (+160.13) / -1.039
aldolase A / 7412 / n / 2 / PhPYPALTPEQK / 2.71 / 0.237 / 1 / h2: # (+160.13) / 2.602
aldolase A / 7457 / n / 2 / PhPYPALTPEQK / 3.08 / 0.228 / 1 / h2: # (+160.13) / -1.559
aldolase A / 7508 / n / 2 / PhPYPALTPEQK / 3.35 / 0.353 / 1 / h2: # (+160.13) / 0.3265
aldolase A / 8049 / n / 3 / PhPYPALTPEQKK / 3.69 / 0.122 / 1 / h2: # (+160.13) / -1.199
aldolase A / 6883 / n / 2 / YTPSGQSGAAASESLFISNhAY / 2.14 / 0.181 / 2 / h20: # (+160.13) / -0.1232
aldolase A / 7056 / n / 2 / YTPSGQSGAAASESLFISNhAY / 4.36 / 0.477 / 2 / h20: # (+160.13) / 3.309
amylo-1, 6-glucosidase, 4-alpha-glucanotransferase / 7309 / y / 2 / LhQELGAK / 2.34 / 0.124 / 2 / h2: # (+160.13) / 0.4756
ATP synthase, H+ transporting, mitochondrial F1 complex, beta subunit / 7126 / n / 3 / ImDPNIVGSEhYDVAR / 3.47 / 0.166 / 2 / m2: Oxidation (+15.99), h11: # (+160.13) / -1.061
ATP synthase, H+ transporting, mitochondrial F1 complex, beta subunit
Continued / 7178 / n / 3 / ImDPNIVGSEhYDVAR / 3.1 / 0.272 / 2 / m2: Oxidation (+15.99), h11: # (+160.13) / -0.3077
Protein name / Scan number / 18O labeled? / Precursor charge / Peptide sequence / SEQUEST XCorr score / SEQUEST DCn score / Number of enzymatic termini / Variable modifications / Delta mass (ppm)
ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 isoform a / 6582 / n / 2 / hTDPVPDPR / 2.21 / 0.15 / 2 / h1: # (+160.13) / -2.009
ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 isoform a / 6630 / n / 2 / hTDPVPDPR / 2.15 / 0.0723 / 2 / h1: # (+160.13) / -1.841
beta-glo / 7148 / y / 2 / VhLTDAEK / 2.1 / 0.105 / 1 / h2: # (+160.13) / -2.329
beta-glo / 7195 / y / 2 / VhLTDAEK / 2.16 / 0.117 / 1 / h2: # (+160.13) / -1.303
beta-glo / 7244 / n / 2 / VhLTDAEK / 2.17 / 0.0719 / 1 / h2: # (+160.13) / -1.21
carbonic anhydrase 3 / 6884 / y / 2 / GDNQSPIELhTK / 2.51 / 0.201 / 2 / h10: # (+160.13) / 0.1348
carbonic anhydrase 3 / 6937 / y / 2 / GDNQSPIELhTK / 2.96 / 0.298 / 2 / h10: # (+160.13) / -0.399
carbonic anhydrase 3 / 6985 / y / 2 / GDNQSPIELhTK / 2.68 / 0.116 / 2 / h10: # (+160.13) / -0.7326
cytochrome c oxidase subunit IV isoform 1 / 7569 / y / 2 / DYPLPDVAhVK / 2.8 / 0.163 / 2 / h9: # (+160.13) / -1.908
cytochrome c oxidase subunit IV isoform 1 / 7516 / n / 2 / DYPLPDVAhVK / 2.66 / 0.0761 / 2 / h9: # (+160.13) / -1.13
cytochrome c oxidase subunit IV isoform 1 / 7617 / n / 2 / DYPLPDVAhVK / 2.59 / 0.117 / 2 / h9: # (+160.13) / -1.13
decorin / 7030 / y / 2 / LhDNEITK / 2.71 / 0 / 2 / h2: # (+160.13) / -1.238
decorin / 7132 / n / 2 / LhDNEITK / 2.1 / 0.0446 / 2 / h2: # (+160.13) / -1.592
enolase 2, gamma / 7024 / y / 2 / GNPTVEVDLhTAK / 3.6 / 0.358 / 2 / h10: # (+160.13) / -0.453
enolase 2, gamma / 7075 / y / 2 / GNPTVEVDLhTAK / 4.53 / 0.377 / 2 / h10: # (+160.13) / -0.1285
enolase 2, gamma / 7122 / y / 2 / GNPTVEVDLhTAK / 3.77 / 0.383 / 2 / h10: # (+160.13) / -1.102
enolase 2, gamma / 7319 / y / 3 / PTVEVDLhTAK / 2.47 / 0.145 / 1 / h8: # (+160.13) / -0.5095
enolase 2, gamma / 7326 / y / 2 / PTVEVDLhTAK / 3.98 / 0.326 / 1 / h8: # (+160.13) / 0.9505
enolase 2, gamma / 7368 / y / 2 / PTVEVDLhTAK / 4.22 / 0.301 / 1 / h8: # (+160.13) / -1.167
enolase 2, gamma / 7185 / n / 2 / GNPTVEVDLhTAK / 3.42 / 0.347 / 2 / h10: # (+160.13) / 2.532
eukaryotic translation elongation factor 1 alpha 2 / 7528 / y / 2 / STTTGhLIYK / 2.7 / 0.086 / 2 / h6: # (+160.13) / -0.623
hemoglobin alpha 1 chain / 7865 / n / 3 / TYFShIDVSPGSAQVK / 3.07 / 0.145 / 2 / h5: # (+160.13) / -1.423
histone cluster 1, H2bl / 2719 / y / 2 / hAVSEGTK / 2.56 / 0.163 / 2 / h1: # (+160.13) / -1.616
Continued
Protein name / Scan number / 18O labeled? / Precursor charge / Peptide sequence / SEQUEST XCorr score / SEQUEST DCn score / Number of enzymatic termini / Variable modifications / Delta mass (ppm)
histone cluster 1, H2bl / 2776 / y / 2 / hAVSEGTK / 3.1 / 0.218 / 2 / h1: # (+160.13) / -2.729
histone cluster 1, H2bl / 6412 / y / 2 / hAVSEGTK / 2.56 / 0.173 / 2 / h1: # (+160.13) / -0.5037
histone cluster 1, H2bl / 6454 / n / 2 / hAVSEGTK / 2.39 / 0.129 / 2 / h1: # (+160.13) / -2.426
histone cluster 1, H4b / 4056 / y / 2 / DAVTYTEhAK / 2.2 / 0.131 / 2 / h8: # (+160.13) / -0.9253
histone cluster 1, H4b / 6021 / n / 2 / DAVTYTEhAK / 2.14 / 0.003 / 2 / h8: # (+160.13) / -0.7708
hypothetical protein LOC295010 / 3970 / n / 2 / kVmTDEEGSK / 2.57 / 0.0538 / 1 / k1: ^ (+160.13), m3: Oxidation (+15.99) / 6.761
hypothetical protein LOC314214 / 6579 / y / 2 / CGDFkAVIVEVAGQ / 2.81 / 0.0526 / 1 / k5: ^ (+160.13) / -3.069
hypothetical protein LOC365377 / 7602 / n / 2 / LLPAAEAhAR / 2.4 / 0.278 / 2 / h8: # (+160.13) / -1.901
hypothetical protein LOC498587 / 4764 / n / 2 / ATIIhAQ / 2.37 / 0.0471 / 1 / h5: # (+160.13) / -0.8735
hypothetical protein LOC498587 / 6886 / n / 2 / ATIIhAQYNTPISmY / 3.37 / 0.301 / 1 / h5: # (+160.13), m14: Oxidation (+15.99) / 0.1512
hypothetical protein LOC498587 / 7891 / n / 3 / DSALSThKPIEVK / 2.89 / 0.182 / 2 / h7: # (+160.13) / -0.3142
malate dehydrogenase, mitochondrial / 7471 / n / 2 / VNVPVIGGhAGK / 1.99 / 0.194 / 2 / h9: # (+160.13) / -0.7631
myosin, heavy polypeptide 4 / 7241 / y / 2 / LYEQhLGK / 2.11 / 0.0616 / 2 / h5: # (+160.13) / -0.9567
myosin, heavy polypeptide 4 / 7296 / y / 2 / LYEQhLGK / 2.5 / 0.227 / 2 / h5: # (+160.13) / -1.218
myosin, heavy polypeptide 4 / 7263 / n / 2 / LhTESGEFSR / 2.64 / 0.331 / 2 / h2: # (+160.13) / -0.1497
myosin, heavy polypeptide 4 / 7304 / n / 3 / LhTESGEFSR / 3.45 / 0.141 / 2 / h2: # (+160.13) / -0.3009
myosin, heavy polypeptide 4 / 7307 / n / 2 / LhTESGEFSR / 2.67 / 0.227 / 2 / h2: # (+160.13) / -0.9813
myosin, heavy polypeptide 4 / 7345 / n / 3 / LhTESGEFSR / 3.99 / 0.199 / 2 / h2: # (+160.13) / -0.4521
myosin, heavy polypeptide 4 / 7350 / n / 2 / LhTESGEFSR / 2.52 / 0.212 / 2 / h2: # (+160.13) / -0.6789
myosin, heavy polypeptide 4 / 7392 / n / 3 / LhTESGEFSR / 3.33 / 0.147 / 2 / h2: # (+160.13) / -1.133
myosin, heavy polypeptide 4 / 7398 / n / 2 / LhTESGEFSR / 2.5 / 0.174 / 2 / h2: # (+160.13) / -1.284
myosin, heavy polypeptide 4 / 7456 / n / 2 / LhTESGEFSR / 2.26 / 0.149 / 2 / h2: # (+160.13) / -0.3009
myosin, heavy polypeptide 4 / 7534 / n / 2 / LhTESGEFSR / 2.29 / 0.118 / 2 / h2: # (+160.13) / 0.1528
myosin, heavy polypeptide 4 / 7645 / n / 2 / LhTESGEFSR / 2.06 / 0.0901 / 2 / h2: # (+160.13) / -2.191
myosin, heavy polypeptide 7, cardiac muscle, beta / 3597 / n / 2 / EQDTSAhLER / 2.29 / 0.101 / 2 / h7: # (+160.13) / -1.262
Continued
Protein name / Scan number / 18O labeled? / Precursor charge / Peptide sequence / SEQUEST XCorr score / SEQUEST DCn score / Number of enzymatic termini / Variable modifications / Delta mass (ppm)
myosin, heavy polypeptide 7, cardiac muscle, beta / 7659 / n / 3 / DLEEATLQhEATAAALR / 3.45 / 0.224 / 2 / h9: # (+160.13) / -1.15
myosin, heavy polypeptide 7, cardiac muscle, beta / 7705 / n / 2 / DLEEATLQhEATAAALR / 5.46 / 0.428 / 2 / h9: # (+160.13) / 0.9515
NADH dehydrogenase (ubiquinone) Fe-S protein 7 / 3337 / n / 3 / AhQSVAATGSPSSTQSAVSK / 2.82 / 0.211 / 2 / h2: # (+160.13) / 0.4862
NADH dehydrogenase (ubiquinone) Fe-S protein 7 / 3420 / n / 3 / AhQSVAATGSPSSTQSAVSK / 3.14 / 0.206 / 2 / h2: # (+160.13) / 0.09803
nebulin / 6568 / y / 2 / hVSDTNYK / 2.2 / 0.108 / 2 / h1: # (+160.13) / -0.9772
nebulin / 6660 / n / 2 / hVSDTNYK / 2.21 / 0.14 / 2 / h1: # (+160.13) / -1.867
PDZ and LIM domain 5 / 2826 / y / 2 / ASQAhVR / 2.8 / 0.139 / 2 / h5: # (+160.13) / -1.506
PDZ and LIM domain 5 / 2878 / y / 2 / ASQAhVR / 2.56 / 0.108 / 2 / h5: # (+160.13) / -1.075
PDZ and LIM domain 5 / 6277 / y / 2 / ASQAhVR / 2.47 / 0.088 / 2 /