Supplemental Figure S1. Aligned ATPaseb flanking exon (A) and intron (B) sequences from two Musculista senhousia specimens (labeled “Msen”), and representative taxa from the NCBI database. Alignment was done with MAFFT v. 6.240, using the E-INS-i setting with a gap-opening value of 1.0. Accession numbers for reference sequences follow the taxon name; Drosophila = D. melanogaster. Only the entries for Haliotis spp. included intron sequence data, all others were cDNA sequences. Highlighted regions are where primers anneal; the grey bar indicates where the intron occurs; intron sequences were excised and are given in section B.
(A) Exon sequence flanking the ATPaseb intron
Mytilus_edulis_AY580269 CGT GAA GGA AAT GAC TTG TAC CAT GAA ATG ATC ACA TCT AAA
Modiolus_americanus_AY580264 CGT GAA GGA AAT GAC TTG TAC CAT GAA ATG ATC ACA TCT AAA
Drosophila_BT120330 CGT GAG GGA AAC GAT TTA TAC AAT GAG ATG ATC GAG GGT GGT
Haliotis_rubra_3_AY043198 ??? ??? ??? ??? ??? ??? ??? ??? ??? ??? ??? ??? ??? ???
Haliotis_conicopora_1_AY043200 ??? ??? ??? ??? ??? ??? ??? ??? ??? ??? ??? ??? ??? ???
Haliotis_laevigata_2_AY043204 ??? ??? ??? ??? ??? ??? ??? ??? ??? ??? ??? ??? ??? ???
Msen_06DWA6 ??? ??? ??? ??? ??? ??? TAC CAT GAG ATG ATC ACC TCA AAA
Msen_06COR10 ??? ??? ??? ??? ??? ??? ??? CAT GAG ATG ATC ACA TCA AAA
Mytilus_edulis_AY580269 GTT ATT AGC TTG ACA GAC GAC ACG TCC AAG GTA TCT TTG
Modiolus_americanus_AY580264 GTC ATC AGT TTA ACA GAT GAC ACA TCA AAG GTA TCC CTT
Drosophila_BT120330 GTT ATT TCA CTA AAG GAC AAG ACC TCA AAG GTG GCT CTC
Haliotis_rubra_3_AY043198 ??? ??? ??? ??? ??? ??? ??? ACC TCC AAG GTT TCT CTG
Haliotis_conicopora_1_AY043200 ??? ??? ??? ??? ??? ??? ??? ACC TCC AAG GTT TCT CTG
Haliotis_laevigata_2_AY043204 ??? ??? ??? ??? ??? ??? ??? ACC TCC AAG GTT TCT CTG
Msen_06DWA6 GTC ATC AGC TTG ACA GAC GAT ACG TCT AAG GTA TCG CTT
Msen_06COR10 GTC ATY AGC TTG ACA GAC GAT ACA TCT AAG GTA TCG CTT
Mytilus_edulis_AY580269 GTA TAT GGA CAG ATG AAT GAG CCC CCA GGA GCA CGT GCC CG
Modiolus_americanus_AY580264 GTA TAT GGA CAA ATG AAT GAA CCA CCA GGA GCA CGT GCC CG
Drosophila_BT120330 GTA TAT GGG CAA ATG AAC GAG CCG CCA GGT GCT CGT GCC CG
Haliotis_rubra_3_AY043198 GTA TAC GGT CAG ATG AAC GAA CC? ??? ??? ??? ??? ??? ??
Haliotis_conicopora_1_AY043200 GTA TAC GGT CAG ATG AAC GAA CC? ??? ??? ??? ??? ??? ??
Haliotis_laevigata_2_AY043204 GTA TAC GGT CAG ATG AAC GAA CC? ??? ??? ??? ??? ??? ??
Msen_06DWA6 GTA TAC GGA CAA ATG AAC GAA CCG CCA GGT GCT CGT GCC CG
Msen_06COR10 GTA TAC GGA CAA ATG AAC GAA CC? ??? ??? ??? ??? ??? ??
(B) ATPaseb intron sequences
H_rubra ------agcactg--tgttt------tgtttcttrattcacacactagtacacaactatg
H_conicopora ------agcactg--tgttt------tgtttcttgattcacacactagtacacaactatg
H_laevigata ------agcacc---tgttt------tgtttcttgattcatrcactagtacacaactatg
Msen_06DWA6 ctgtaaaatgttgaatgtttatcatctgttaattgttcaaattaccga-aaaaaagttat
Msen_06COR10 ctgtgaaatgttgaatgtttatcatctgtaaattgttcaaactaccaa-aaaaaacctta
H_rubra aataatg---gataatacatacctgcttt------
H_conicopora aataatg---gataatacatacctgcttt------
H_laevigata aataatg---gataatacatacctgcttt------
Msen_06DWA6 tatggtgtcagaagatttatgaatgattttcatgaata------ttgtcacttctgtaat
Msen_06COR10 aatgatg---aaaagtatatacatgtatatcaaaattacaacattaatcacccttacctt
H_rubra ------
H_conicopora ------
H_laevigata ------
Msen_06DWA6 ttaagag------ggac------agatgttgcaataaa
Msen_06COR10 taaatagattgccttgaaatactggtaacttttaggactgccttgaaatactagtatgaa
H_rubra ------
H_conicopora ------
H_laevigata ------
Msen_06DWA6 tgatgaaaagaatatattttt------atactagagggaagc
Msen_06COR10 -ggtcagaactatattttgttggtaacttttaggactgccttgaaatactagaatgaagg
H_rubra ------ttctaagaaaatga------
H_conicopora ------ttctaagaaaatga------
H_laevigata ------ttctaagaaaataa------
Msen_06DWA6 tcagaactatattttgttggtaacttataagaaagcaaaaaattgttggaatggaattaa
Msen_06COR10 ttagaactatattttgttggwaayttttaggacagcaaaagattgttggaatggaattta
H_rubra ----agagcattaac------atgattaa
H_conicopora ----agagcattaac------atgattaa
H_laevigata ----agagcattaac------atgattaa
Msen_06DWA6 aaccaaagtattagttggccaaaaactatcaaacttgcttgattttctaaaaatgaccaa
Msen_06COR10 aaccaaagtattagttggccaaaaactatcaatcttgcttgttttctaaagaatgacaaa
H_rubra cat------ggtcctttt------
H_conicopora cat------ggtcctttt------
H_laevigata cat------ggtcctttt------
Msen_06DWA6 tattcacagttctacttgtgttcttcacaatcaaaaacctttattctaacttttaatgac
Msen_06COR10 tccc-----acctacttgtgttcttaacaatcaaaaatcttttttctaacttttaatgac
H_rubra ------caggta
H_conicopora ------caggta
H_laevigata ------caggta
Msen_06DWA6 tacaatgtacttcaaacaattttgacaggta
Msen_06COR10 ------tactacaaacaattttgacaggta