SUPPLIMENTARY FILE

Identification and phylogenetic characterization of novel Lactobacillus plantarum species and their metabolite profile in grass silage

Mariadhas Valan Arasu, Min Woong Jung, Da Hye Kim, Hyung Soo Park, Soundarrajan Ilavenil, Naif Abdullah Al-Dhabi, Ki Choon Choi

M.Valan Arasu, M-W. Jung, H-S. Park, S. Ilavenil, K-C Choi*

Grassland and forage division, National Institute of Animal Science, RDA, Seonghwan-Eup, Cheonan-Si, Chungnam, 330-801, Republic of Korea, Tel: +82-41-580-6752, Fax: +82-41-580-6779 , E-mail:

D-H Kim

The United Graduate School of Agricultural Sciences, Tottori University, Tottori-Shi, 680-8553, Japan

N. A. Al-Dhabi

Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, Riyadh 11451, Saudi Arabia

Table S1

Biochemical and physiological characteristics of Lactobacillus plantarum KCC-10 and Lactobacillus plantarum KCC-19 isolated from silage.

Tests / L. plantarum KCC-10 / L. plantarum KCC-19 / L. plantarum KACC 91016
Growth under anaerobic conditions / + / + / +
Gram staining / positive / positive / positive
Shape and growth / rod / rod / rod
Motility / + / + / +
Catalase / + / + / +
Amylase / + / + / +
Cellulase / - / - / -
Lipase / - / - / -
Chitinase / - / - / -
Gelatinase / - / - / -
Gas from glucose / + / + / +
Gas from fructose / + / + / +
Gas from lactose / + / + / +
Growth at temperature (◦C)
20 / + / + / +
25 / + / + / +
30 / + / + / +
35 / + / + / +
40 / + / + / +
50 / - / - / -
Growth in NaCl
1 / + / + / +
2 / + / + / +
3 / + / + / +
4 / + / + / +
5 / + / + / +
6
Growth at pH
2 / + / + / +
2.5 / + / + / +
3 / + / + / +
3.5 / + / + / +
5 / + / + / +
6 / + / + / +
7 / + / + / +
8 / + / + / +
Growth in presence of oxgal / + / + / +

+: Positive (more than 90%); −: Negative (more than 90%); KACC- microbial culture collection at the Rural Development Administration (RDA), Suwon, South Korea.

Table S2.

API 50 CH fermentation patterns of Lactobacillus plantarum KCC-10 and Lactobacillus plantarum KCC-19 isolated from silage.

+: Positive (more than 90%); −: Negative (more than 90%); KACC- microbial culture collection at the Rural Development Administration (RDA), Suwon, South Korea.