Supplementary File 1 CLUSTAL W(1.81) Multiple Sequence Alignment of Runx Gene Orthologs

Supplementary file 1 CLUSTAL W(1.81) multiple sequence alignment of Runx gene orthologs. Runx domain indicated by a black line on top of the sequences. The positions of introns on the protein sequences within the conserved Runx domain were indicated in pink background. Introns of Blan_Runt were not labeled because its genome sequence is not available.

Dmel_lz -MHLHLLAAEKTPPSPSPNPTPTPSASPSGHTGAAGESQLDLASQTESQLQLGLPLASGY

Aaeg_lz -MHL------PSGGPTAIIPP------

Tcas_lz -MHLPVS-----LSSCT------

Amel_lz -MKSAVPRPPRITTDCRGSLWAIYGD------

Nvit_lz -MHLPVG-----SVSCSGESFGSLSQ------

Dmel_CG42267 ------

Aaeg_CG42267l ------

Tcas_CG42267l ------

Amel_CG42267l -MTNPLRDAR----PEDRPLGLDYPDPR------

Nvit_CG42267l -MTNPARDARGGVAVENYAGGGSDPSSR------

Dmel_CG34145 -MEHEG-ANSNNYK------

Aaeg_CG34145l ------

Tcas_CG34145l ------

Amel_CG34145l -MDIFGRCNGGGGG------

Nvit_CG34145l -MDIFGRCNGGAGLGVNSVPATAAVV------

Dmel_run ------

Aaeg_run ------

Tcas_run ------

Amel_run ------

Nvit_run ------

Mmus_Runt1 ------

Mmus_Runt2 MLHSPHKQPQNHKCGANFLQEDCKKALAFKWLISAG------HYQPPRPTESFK

Mmus_Runt3 ------

Nvec_Runt ------

Spur_Runt1 ------

Spur_Runt2 ------

Blan_Runt ------

Dmel_lz GLGLGLGLGLGLGLGQELVADHSTTVAPVSVAGPGRLGRSINGSGGSHHHHHLHHHYSPY

Aaeg_lz ------ISNNEANVTPTAST------EWDIY

Tcas_lz ------

Amel_lz ------GDDRQADIRKPDSGGSSATLLEYPGHPSCEPEAEKQLLEKK

Nvit_lz ------QHHHHQHHQQ------HHQHHQQHHQLQQEQD

Dmel_CG42267 ------MHISAEVSSTTSNQIQQQQQQHQQQQQHQLLQHQQQQTAT

Aaeg_CG42267l ------MHLATPEVTSTTN------

Tcas_CG42267l ------MGQSESCGPP------PQHHSRDFSSHF

Amel_CG42267l ------LVRNGHQEHQGNTTAHQ------IGAARI

Nvit_CG42267l ------LQQSHHQQHLPHHSSHHHHHHHHHQQQQHQHRHQQDQQHSSSGSNRL

Dmel_CG34145 ------MHLTTTSSNS------TASNANNNNNNTANNN

Aaeg_CG34145l ------

Tcas_CG34145l ------MHLTANSQTA------TGTAAS------

Amel_CG34145l ------SSTVSGSG------AGSVRGSGNESSCGTA

Nvit_CG34145l ------AQSATTRSANNL------AGPVVGPEHASTVGNT

Dmel_run ------MHLPAGPTMVANNTQVLAAAAAAAAAAAAAVAQGPGPQQS

Aaeg_run ------

Tcas_run ------MHLP------

Amel_run ------MHLPEGP------

Nvit_run ------MHLPEGP------

Mmus_Runt1 ------MRIPVDAST--SRRF

Mmus_Runt2 AASSIYNRGHKFYLEKKGGTMASNSLFSAVTP------CQQSFFWDPST--SRRF

Mmus_Runt3 ------MASNSIFDSFPN------YTPTFIRDPST--SRRF

Nvec_Runt ------MTTTENQGTR--

Spur_Runt1 ------MHITDVNVDHLLS------STAPLANHPSKDPVRRN

Spur_Runt2 ------

Blan_Runt ------MLIPTPSTLDSRRF

Dmel_lz HHAHPYHPPHPHAPHHHHHHHPPYPYPPAGPHPPAMVTSSSTSPTGNGWSSSTGDFKGIT

Aaeg_lz KDEEMLEP------PAG------

Tcas_lz ------VSEEAWPKSS---G------

Amel_lz LLVAAIAEESPWSVQIEEASEITTSCPEGTCLGDEGRQLADGMHLPVGSVGCGENYATVG

Nvit_lz NGNLVALQPAQYQQQIQEQHQQQQQQVEGQRQ------

Dmel_CG42267 TTTTKRRNAESSASSNNNNNNNTSTTN------

Aaeg_CG42267l -PTSMELNGNSGGGLN------TDSVN------

Tcas_CG42267l RAVRWPRHAAKKLTSMHLATGEVTSTTG------

Amel_CG42267l RRTRTSSTRRR----MHLANEVPPQGS------

Nvit_CG42267l RRSRTSSLRRNGSRTMHLSGETQAQQQ------

Dmel_CG34145 NTSSNNNTTNTNGSSNNNTSGNNNNSSGNNSN------

Aaeg_CG34145l ------

Tcas_CG34145l ------

Amel_CG34145l SG------ASESGGTSDRMQLTGASAP------

Nvit_CG34145l GGGGNGNNNGPAAPAEAATSQSRMQLTGASAP------

Dmel_run SNATTASAIAINPAQSLANTSTHSASSTGSSTPDL------

Aaeg_run ------

Tcas_run ------INAQQ------

Amel_run ------

Nvit_run ------

Mmus_Runt1 TPPSTALSPG-----KMSEALPLGAPD------

Mmus_Runt2 SPPSSSLQPG-----KMSDVSPVVAAQQQQQQQQQQQQQQQQQQQQQQ------

Mmus_Runt3 TPPSTAFPCGGGGGGKMGENSGALSAQ------

Nvec_Runt ------

Spur_Runt1 NLHNS------YKMAEG------

Spur_Runt2 ------MTEV------

Blan_Runt SPFAD---PG-----KMGD------

Dmel_lz AVATATGGGVGGATQGATASTGATAAEVLAVSSSASVGSSSPTGGASNGTAHSGHSGHTG

Aaeg_lz ------ANNNAENSSPSPGG------SG------

Tcas_lz ------MCEP----SSGYVSNNQ------

Amel_lz SHEGAGPCGPSLAPLQGVNTRYQQADDADAGGGGGEMSEGAAVG------

Nvit_lz ------EEAAEPGPTGAAGEMSEAAAVG------

Dmel_CG42267 ------NNNTNNNNNSTTNNN------

Aaeg_CG42267l ------TNGTTNDGTSKHQP------

Tcas_CG42267l ------HYDRSYGN------

Amel_CG42267l ------TGG--NNLPDYA------

Nvit_CG42267l ------AAAPASQLPDYA------

Dmel_CG34145 ------TEQNTPPTPAQLLNEAYTK------

Aaeg_CG34145l ------

Tcas_CG34145l ------PEGSSVINDTYTK------

Amel_CG34145l ------GVAASPESGVSPLTDAYTK------

Nvit_CG34145l ------GNAASPESGVSPLADAYTK------

Dmel_run ------STNNTSSSSNATTSPQNSAKM------

Aaeg_run ------

Tcas_run ------

Amel_run ------

Nvit_run ------

Mmus_Runt1 ------GGAALAS------

Mmus_Runt2 ------QQQQQQQEAAAAAAAAAAAAAAAAAAVP------

Mmus_Runt3 ------ATAGPGG------

Nvec_Runt ------

Spur_Runt1 ------GQRNKASSV------

Spur_Runt2 ------GQR-KGSSP------

Blan_Runt ------PHRKVHP------

Dmel_lz GHSSSTASNNNNNGASNSNSNNNNAVHQDLLWMERLVQKRQQEHPGELVRT------

Aaeg_lz ------DLWWIERMVMEAQQEYPGELVRT------

Tcas_lz ------DIWWTEHLIHEIQTEHPGELVRT------

Amel_lz ------ELWWTERLVGEAQAEHPGELVRT------

Nvit_lz ------ELWWTERLVGEAQAEHPGELVRT------

Dmel_CG42267 ------NNNNNNNVKTKPVDTSPYLTPENLIERTVDVLLAEHPGELVKT------

Aaeg_CG42267l ------LTAESLAERTIEGLLADHPGELIRT------

Tcas_CG42267l ------LTAEMLAERTIDGLLAEHPGELVRT------

Amel_CG42267l ------LTADLLTERTLDGLFAEHPGELVRTEKKKRIDGL

Nvit_CG42267l ------LTADLLAERTLDGLLAEHPGELVRT------

Dmel_CG34145 ------MTSDILAERTLGDFLTEHPGELIRT------

Aaeg_CG34145l ------MTSDILAERTLGDFLSEHPGELIRT------

Tcas_CG34145l ------MTSDILAERTLNDFLSEHPGELIRT------

Amel_CG34145l ------MTSDILAERTLGDFVSEHPGELVRT------

Nvit_CG34145l ------MTSDIFAERTLGDYMSEHPGELVRT------

Dmel_run ------PSSMTDMFASLHEMLQEYHGELAQT------

Aaeg_run ------MADMFSNLHDMLQEYHGELVQT------

Tcas_run ------PSIMDMYASLQETLQEYHGELVQT------

Amel_run ------LGMD--INAMHETLQACHGDLVRT------

Nvit_run ------IGMENHFSAIHDALRACHGELVQT------

Mmus_Runt1 ------KLRS--GDRSMVEVLADHPGELVRT------

Mmus_Runt2 ------RLRPPHDNRTMVEIIADHPAELVRT------

Mmus_Runt3 ------RTRP--EVRSMVDVLADHAGELVRT------

Nvec_Runt ------KIKG---ERSLVEALAEYPGELVKT------

Spur_Runt1 ------FKGGERSIVDALSEYPGELVKT------

Spur_Runt2 ------CKGGERSLVDALSDYPGELVKT------

Blan_Runt ------HFKG---DRGLVDALADHPGELVRT------

Runx domain

Dmel_lz ------SNPYFLCSALPAHWRSNKTLPMAFKVVALAEVGDGTYVTIRAGNDENCCAELR

Aaeg_lz ------GSPYFLCSALPNHWRSNKTLPSAFKVVSLGDVCDGTMVTIRAGNDENFCGELR

Tcas_lz ------GSPYFLCSALPTHWRSNKTLPVAFKVVALGDIGDGTIVTVRAGNDENCCAELR

Amel_lz ------GSPYFLCSQLPTHWRSNKTLPVAFKVVALGEVVDGTLVTVRAGNDENCCAELR

Nvit_lz ------GSPYFLCSQLPTHWRSNKTLPAAFKVVALGEIGDGTLVTVRAGNDENCCAELR

Dmel_CG42267 ------GSPHVVCTTLPTHWRSNKTLPIAFKVLALGEVMDGTIVTIRAGNDENFCGELR

Aaeg_CG42267l ------GSPHVVCTVLPNHWRSNKTLPVAFKVVALGDVGDGTMVTVMAGNDENFCGELR

Tcas_CG42267l ------GSPHVVCTVLPPHWRSNKTLPVAFKVVALGDVGDGTVVTVRAGNDENYCAELR

Amel_CG42267l IRQRWRTGSPHVVCTVLPAHWRSNKTLPVAFKVVALGEVGDGTLVTVRAGNDENCCAELR

Nvit_CG42267l ------GSPHVVCTVLPSHWRSNKTLPVAFKVVALGEVGDGTLVTVRAGNDENCCAELR

Dmel_CG34145 ------SSPLFVCTVLPPHWRSNKTLPVAFKVVSLGDIMDGTMVTVRAGNDENYCAELR

Aaeg_CG34145l ------GSPLFVCTVLPPHWRSNKTLPVAFKVVALGDVGDGTMVTVRAGNDENYCAELR

Tcas_CG34145l ------GSPLFVCTVLPPHWRSNKTLPVAFKVVALGDVGDGTVVTVKAGNDENYCAELR

Amel_CG34145l ------GSPHLVCTVLPAHWRSNKTLPVAFKVVALGEVGDGTLVTVRAGNDENCCAELR

Nvit_CG34145l ------GSPHLVCTVLPAHWRSNKTLPVAFKVVALGEVGDGTVVTVRAGNDENCCAELR

Dmel_run ------GSPSILCSALPNHWRSNKSLPGAFKVIALDDVPDGTLVSIKCGNDENYCGELR

Aaeg_run ------GSPAVLCSALPTHWRSNKSLPCAFKVIALDDIQDGTVVTIKAGNDENCHAELR

Tcas_run ------GSPAVLCSVLPSHWRSNKSLPIAFKVVALDEVRDGTTVTLKAGNDENYCAELR

Amel_run ------GSPAILCSALPSHWRSNKSLPVAFKVVALDDVSDGTLVTIRAGNDENCCGELR

Nvit_run ------GSPAILCSPLPSHWRSNKSLPVAFKVVALDEVSDGTLVTISAGNDENFCGELR

Mmus_Runt1 ------DSPNFLCSVLPTHWRCNKTLPIAFKVVALGDVPDGTLVTVMAGNDENYSAELR

Mmus_Runt2 ------DSPNFLCSVLPSHWRCNKTLPVAFKVVALGEVPDGTVVTVMAGNDENYSAELR

Mmus_Runt3 ------DSPNFLCSVLPSHWRCNKTLPVAFKVVALGDVPDGTVVTVMAGNDENYSAELR

Nvec_Runt ------DSPNFVCSVLPSHWRCNKTLPVAFKVVSLGDIPDGVIVSIAAGNDENFAAELR

Spur_Runt1 ------ESPNFACSVLPNHWRCNKSLPVAFKVVSLGETKDGTMVTIAAGNDENYCAELK

Spur_Runt2 ------ESPNFICSLLPPHWRSNKSLPVAFKVVSLGETKDGTVVTIGAGNDENCCAELK

Blan_Runt ------DSPNFVCSVLPSHWRCNKTLPVPFKVVALGDIPDGTLVTVMAGNDENYSAELR

Dmel_lz NFTTQMKNDVAKFNDLRFVGRSGRGKSFTLTITVATSP-PQVATYAKAIKVTVDGPREPR

Aaeg_lz NCTAVMRNQVAKFNDLRFVGRSGRGKSFTLSITVSTTP-PQVTTYTKAIKVTVDGPREPR

Tcas_lz NSTAVMKNQVAKFNDLRFVGRSGRGKSFSITITVSTTP-PQVATYTKAIKVTVDGPREPR

Amel_lz NSTTLMKNQVAKFNDLRFVGRSGRGKSFSITITVSTTP-PQVATYTRAIKVTVDGPREPR

Nvit_lz NSTALMKNQVAKFNDLRFVGRSGRGKSFSITITVSTTP-PQVATYTRAIKVTVDGPREPR

Dmel_CG42267 NCTAVMKNQVAKFNDLRFVGRSGRGKSFTLTIVISTNP-IQIATYTKAIKVTVDGPREPR

Aaeg_CG42267l NCTAIMKNQVAKFNDLRFVGRSGRG------

Tcas_CG42267l NCTAVMKNQVAKFNDLRFVGRSGRGKSFTLSIIVSTTP-PQIATYNKAIKVTVDGPREPR

Amel_CG42267l NSTAVMKNQVAKFNDLRFVGRSGRGKSFTLTIMLQTSP-PQVATLSKAIKVTVDGPREPR

Nvit_CG42267l NSTALMKNQVAKFNDLRFVGRSGRGKSFTLTIMLQTSP-PQIATLSKAIKVTVDGPREPR

Dmel_CG34145 NCTAVMKNQVAKFNDLRFVGRSGRGKSFTLTITVSTNP-PHIATYNKAIKVTVDGPREPR

Aaeg_CG34145l NCTAVMKNQVAKFNDLRFVGRSGRGKSFTLTITISTSP-PQVATYNKAIKVTVDGPREPR

Tcas_CG34145l NSTAVMKNQVAKFNDLRFVGRSGRGKSFTLTIMVSTSP-PQVATYNKAIKVTVDGPREPR

Amel_CG34145l NSTALMKNQVAKFNDLRFVGRSGRGKSFTLTITVSTTP-PQVATYTKAIKVTVDGPREPR

Nvit_CG34145l NSTALMKNQVAKFNDLRFVGRSGRGKSFTLTITVSTTP-PQVATYAKAIKVTVDGPREPR

Dmel_run NCTTTMKNQVAKFNDLRFVGRSGRGKSFTLTITIATYP-VQIASYSKAIKVTVDGPREPR

Aaeg_run NCTAIMKNQVAKFNDLRFVGRSGRGKSFSITITISTYP-CQIATYTKAIKVTVDGPREPR

Tcas_run NCTAVMKNQVAKFNDLRFVGRSGRGKSFTLTITISSEPYYQIATYNKAIKVTVDGPREPR

Amel_run NCTAVMKNQVAKFNDLRFVGRSGRGKSFSLTIQISTVP-FQVATYTKAIKVTVDGPREPR

Nvit_run NCTAVMKNQVAKFNDLRFVGRSGRGKSFSLTIQISSVP-FQVATYTKAIKVTVDGPREPR

Mmus_Runt1 NATAAMKNQVARFNDLRFVGRSGRGKSFTLTITVFTNP-PQVATYHRAIKITVDGPREPR

Mmus_Runt2 NASAVMKNQVARFNDLRFVGRSGRGKSFTLTITVFTNP-PQVATYHRAIKVTVDGPREPR

Mmus_Runt3 NASAVMKNQVARFNDLRFVGRSGRGKSFTLTITVFTNP-TQVATYHRAIKVTVDGPREPR

Nvec_Runt NATAVMKNQVARFNDLRFVGRSGRGKTFSLTITVKTEP-PQVATYCRAIKVTVDGP----

Spur_Runt1 NNTAVMKNQVARFNDLRFVGRSGRGKSFTLSIFIYTNP-PQIATYNRAIKVTVDGPREPR

Spur_Runt2 NNIAVMKNQVARFNDLRFVGKSGRGKSFTLSIFVYTNP-PQIATCNRAIKVTVDGPRPRR

Blan_Runt NNQAVMKNQVARFNDLRFVGRSGRGKSFTLTITVFTSP-PQVATYHRAIKVTVDGPREPR

Dmel_lz ----SKTSP------

Aaeg_lz ----SKTSE------

Tcas_lz ----SKTSE------

Amel_lz ----SKTRQ------

Nvit_lz ----SKTNA------

Dmel_CG42267 ----SKVRHQGFHPFAFGPQRFGPDPLMAGLPFKLPGFAHHLVGMHSHLHAPDWRAHMAL

Aaeg_CG42267l ------SMVGLRLD---FQVHIFF--TTMRPPIRPIL---

Tcas_CG42267l ----SKTRQ------

Amel_CG42267l ----SKTNP------

Nvit_CG42267l ----SKTRH------QAFHPFHFGP

Dmel_CG34145 ----SKTRQ------

Aaeg_CG34145l ----SKTRQ------

Tcas_CG34145l ----SKTSS------

Amel_CG34145l ----SKTSE------

Nvit_CG34145l ----SKTRQ------

Dmel_run ----SKQSY------

Aaeg_run ----SKQNF------

Tcas_run ----TKSNY------

Amel_run ----SKSNY------

Nvit_run ----SKSNY------

Mmus_Runt1 ----RHRQK------

Mmus_Runt2 ----RHRQK------

Mmus_Runt3 ----RHRQK------

Nvec_Runt ------

Spur_Runt1 ----RPKPK------

Spur_Runt2 ELYLRPKSK------

Blan_Runt ----RHRQK------

Dmel_lz ------TGGPHY------RALGLGQRPYIDG-FPSTKALHELESLRRSAKVAAVTTAA

Aaeg_lz ------

Tcas_lz ------YLPWIL------LHREPWIIQRN------

Amel_lz ------THIPGLARVPALPRVPGLTRGTFPDAPSPFTPYLRDSEPYRRNKHHVGNN---

Nvit_lz ------LLDLVPGQQHQQFRALGLGQRPYLDGPNSFTNHLRELE-YRRSKHHPHHHHHH

Dmel_CG42267 GGRPAAFTAAPFFGHHAAAFPTASGLRGLSGDSQQHQQQQQQHQLATVGAAHSTTSPEGS

Aaeg_CG42267l --HRIWSTVGIFNNEPGGSNNDG----GSVINSPRISDSNDVHNALGNCNPHSTTSPKSS

Tcas_CG42267l ------QGFHHFPFGPRTFAPDPLAGSLPFKLSVGQRSRAAGDKATEVMSERVDSKTPH

Amel_CG42267l ------LD-----CTGLQHLGLEQG-----ASQGWGGLPRGSLPPHCLPPPPGHHSH

Nvit_CG42267l RPFP-FGHPQDPLGFKLSGLQHLGLDQGAAAAAAAQGWGGLSRGGLPPHCLPPPPGHHSH

Dmel_CG34145 ------QQQFHFAFGQRPFHFSTDPLSGFRMPPIGNCQSASNTHWGYGSAASAYSPYLA

Aaeg_CG34145l ------QQQFHFAFGQRPFHFPADPLSSFRMPPIG------

Tcas_CG34145l ------YG-LYIHMSTNSHWGYG--AAG------AYSPYFT

Amel_CG34145l ------YHWQQQQFH--AFAFASQRGGPFFASPLVDP------LQ

Nvit_CG34145l ------QQPFH--PFAFASQRGGPFFASPLVDP------LQ

Dmel_run ------GYPH-----PGAFNPFMLNPAWLDAAYMTYGY----ADYFRHQAAAQAAQVHH

Aaeg_run ------AYGH-----PGAFNPFMLNPGWIDAAYMNYAW----SDYFRQHQQLQAQQQAS

Tcas_run ------QYGYGLPGMPAGFNPFLLNPGWLDAAYMSYTW----PDYFRARTNVPTQTNLH

Amel_run ------QYGH------GFPGLGLLNPWVDVAYLGHAWHLPHPAFVKGTIPMPSTDLFP

Nvit_run ------QYGP------GFPALGLLNPWLDAAYFSHAWHLPHPALAKGSIGMPHTDLFG

Mmus_Runt1 ------LDDQTKPGSLSFSERLSELEQLRRTAMRVSPHHPAPTPNPRASLNHS-TAFN

Mmus_Runt2 ------LDD-SKPS--LFSDRLSDLGRIPHPSMRVG----VPPQNPRPSLNSAPSPFN

Mmus_Runt3 ------IEDQTK----AFPDRFGDLR------MRVT----PSTPSPRGSLSTT-SHFS

Nvec_Runt ------

Spur_Runt1 ------DQESRLMPP-PIINTGHPHPFGEINPHHPNHHIGRQQSYQNQGRMPRSYPLSP

Spur_Runt2 ------HDDPLCFS-SIIDTGHAHSYGLVISHHDN-QIGRQQPFVNQGWMPGSYPLTS

Blan_Runt ------LEEQKHAL--SFSERLSELGLERLRHSHIAQ---PLPPAIRTPLIEAPPGHHP

Dmel_lz AAAATAASAAN------AVAAAAAAVAVTPTGGGGGVAAGGVAGGAGAGLVQQL

Aaeg_lz ------

Tcas_lz ------

Amel_lz ------VT------ANSVSG-ACTNP------NNTDPTASPASSA----HL

Nvit_lz QQQQQQQQLAT------AQLQSPVSGDNA------NVNSSTTSPSSGS----HL

Dmel_CG42267 PTTTTTSGTQLS------AFVQPPMTSSPPPVTSLQHDNNNNNSNNNNSSSHIDA

Aaeg_CG42267l PNPSSDTLQRVS------SNTTNAKLSS------DADYDREDLEDDN------

Tcas_CG42267l WSGGAASDLIG------IAHHLAGLPGPPPEWAMLGGRHPYPPGPFVPHHHPHFP

Amel_CG42267l THPPAAGASAFNPFHHSIEQRSPRLPGPNSEPSRNDLGPISVSVREVTPTTSPGNPGPGL

Nvit_CG42267l AHPAAAAAAAA------VPQPTTAAAAADGTATAATAAAAAVAASAFNPFHHV

Dmel_CG34145 SSGLSSCTTPT------SAQFNNPALGFTCSSNDQSNNQDFGGATNRDC--VPM

Aaeg_CG34145l ------D------GGR------VAV

Tcas_CG34145l PSTLGSCAAPT------ASQFNTPALGFSGSTPDQTSTQDAFGSTNSDACLFIV

Amel_CG34145l PLPNPLQPLPN------PLQPRDPLSSFRHAMPANCQNMSQFGLTASNS-----

Nvit_CG34145l PLPNPLQSLPN------PLQPRDPLSSFRHAMPGNCQNMSQFGLTAGNS-----

Dmel_run PALAKSSASSV------SPNPNPSVATS----SSSAVQPSE------YPHPA

Aaeg_run QPNPVTAAGKG------RNSSNTQLFSSSLFLAGSPTLPTPNGTTGPLLPTPP

Tcas_run ASLIKGAT--Q------LPPTNGEFYLP------PQP------QFHPP

Amel_run PTFPPSVLPS------YPF-DH-VKY------P

Nvit_run PSFPPTVLPTG------YPLTDHNVKYN------STTA

Mmus_Runt1 PQPQS------QMQDARQIQPSPPWSYDQSY-QYLGSITSSSVHPATP

Mmus_Runt2 PQGQS------QITDPRQAQSSPPWSYDQSYPSYLSQMTSPSIHSTTP

Mmus_Runt3 SQAQT------PIQ------

Nvec_Runt ------

Spur_Runt1 TSGSY------DNIQHQGQASKPWSYYNPYQSSVAQLSDTSILSAQIK

Spur_Runt2 TSCD------NQPEFHTNHYTPSVQQSTGNPILPPQIK

Blan_Runt PPSAYTPGNP------QIQATQDGASRSPPS-SWPYQPYQPFMGAPMPPPAMPP

Dmel_lz SSNYSSPNSTINSDCQVYKPNAPHIQAAEMMGAGEWTNGSSSSAAAYYHSHAHHPHAHHA

Aaeg_lz ------

Tcas_lz ------

Amel_lz GP--NSG-SSAHQDCYKHSPQHGEK------RKQRWIR------

Nvit_lz TN--TTGGATAHQDCYKHSPQHGDTSVGTGGGGGGGSNSGATTATEWAYPSAAPSYPAPA

Dmel_CG42267 GFESDSISVTGSPRKSLGSPLTHDEEEAEAEAEAEAEAEAEAEEAEVGGLSRNG------

Aaeg_CG42267l ----DQISVTGSDQGSTG------QAENDRR------

Tcas_CG42267l PHMFAALDRPINTSPRIANEPS------

Amel_CG42267l LTTATVAAPTPPAEPTTTTTTPSPSSGHHSHFQGLHLLGATGSIFGSIFAP------

Nvit_CG42267l AAAAAIEQRSPRLPATAN------GLHLLGATNSLFGSIFAP------

Dmel_CG34145 LPDSTASDLDQHLSSLVGSTSGQMTHHSLLGAGGQTSISSTVNGASGGGSAGAGTAGGGA

Aaeg_CG34145l MGEMKTTSRKDPLFSQIG-----YSHK------

Tcas_CG34145l LPDTSTTDLDQHLG-LVSSQN--HTNHS------QSSTHTS------

Amel_CG34145l WGYGSTAGYAGYLPGPLSSCA------AQASFPPP------

Nvit_CG34145l WSYGSTAGYAGYLPGPLSSCA------AQTPFAAP------

Dmel_run AAVAAAAG-QPAA-MMPSPPG------

Aaeg_run ADFMSLSGAQPTPNGLPTPTGP------

Tcas_run HNFLPPNG------

Amel_run AEYATTLP------

Nvit_run ADFASSMS------

Mmus_Runt1 ISPGRASGMTSLS------AELSSR-LSTAPDLTAFG------

Mmus_Runt2 LSSTRGTGLPAITDVPRRISDDDTATSDFCLWPSSLSKKSQAGASELGPFS------

Mmus_Runt3 ------GSSDLNPFS------

Nvec_Runt ------

Spur_Runt1 TEPTELALLGQQNSTLQQYPKPDS-----LYPTSITRSSEVQDPRFVYPS------

Spur_Runt2 TEPPELDLVAVQQ------NQPQFIYPT------

Blan_Runt HTTAAATIERIS------PEMAGRLPTTMPEVITQR------

Dmel_lz HAHLQHQMALPPPPPPPAAAPVSVG------VGGNGATMGMGMGVGVGMGMNHYGGGY

Aaeg_lz ------

Tcas_lz ------

Amel_lz ------KKMDFEIVAVLPSDTSQQDTYT

Nvit_lz GSAAGGAATAGGGSGSGSYSPIPAGAFSYPGEALAHHPTTEPVPLPTVLPSDG-QQNAYT

Dmel_CG42267 --GGIQGPLHSESSPGSGGAFTALIQR---SGKNPTELFG--GFAAAGGNHFAPSGHSFN

Aaeg_CG42267l ------SPLLGGAFTSLIQKNHVKMKGGLDIFHNGAFTAGSYSPQVQQPHPLN

Tcas_CG42267l ------STSLGPISINCTPAHSTTSPKTSPTQVGLLTTAGAPLSPPEDDISVTAS

Amel_CG42267l ------LLPQSSWLYNPLYHTQQYVLEPEWHALALRMAQQRLQRPDQARVEETR

Nvit_CG42267l ------LLPQSSWLYNPLYHTQQYILEPEWHALALRMAQQRLQRPDQARLEDLR

Dmel_CG34145 GSGGGAGGGAGGNSILVPRYHTNASNEYNVHSSQNGPRSLSD-SSQAESPVQEDLLTTNT

Aaeg_CG34145l ------

Tcas_CG34145l ------SLLVPRYSTNHS-----DFGLSGPRSLSDNSSAAESPVQDDILST--

Amel_CG34145l ------PPPPPPPPP----SSSSLASFAGAASMN-TPTAPDSTAG------

Nvit_CG34145l ------PPPPPPPPTGISSTGSALTSFTGGTAMNNTPAAPDSTAGNNGPATVP

Dmel_run ------AAPATPYAIPQFPFNHVAAAAAAKAATPHAFHPYNFAAAAGLRARNAA

Aaeg_run ------VLPNGATYLPQFPFGPHADLHLKSPFLPYDISPLRANGLRGPHQLSAT

Tcas_run ------ILPQLSLS------DSPLLRPSVGPMDQLSLR------

Amel_run ------PKSS------SSSSAQAT---IPTSPSR------

Nvit_run ------HKSSSIHSMTPPPTTTPTAPPTSLLLPNSPSR------

Mmus_Runt1 ------DPRQ----FPTLPS------ISD------PRMHYP-----GAFT

Mmus_Runt2 ------DPRQ----FPSISS------LTESR----FSNPRMHYP-----ATFT

Mmus_Runt3 ------DPRQFDRSFPTLQS------LTESR----FPDPRMHYPGA-MSAAFP

Nvec_Runt ------

Spur_Runt1 ------TPAAVSSVSFTPSSMSVLSSGVESPRTILPMTPNPFPLSSQ---DIFSS

Spur_Runt2 ------QPGDTPFYPSQSSSMSGVSPGEDPQRTLLPMTPNQLPLPSQPAQDVFSS

Blan_Runt ------FPGDIPLRFPADSQFSYPDLRFSDPR----LSDPRLLYPAAGATGFTAY

Dmel_lz DSANSLEAGQYAAHLPAVLP------EMHGHGF------ATDP

Aaeg_lz ------

Tcas_lz ------

Amel_lz PNCVSMYPSHGASS-TSSLP------LVPAKSS------DPDI

Nvit_lz PNCVSMYPSGAASSTTSSLA------LIQNPSSVKPLVDTSSDM

Dmel_CG42267 PALAAQLFLQSPLLPQ------SSQWLYTQLYGS

Aaeg_CG42267l HALAAQLFFQNPLIPQ------PNHWLYNQLYGN

Tcas_CG42267l PTPSPQPPPAFPG------APPPPIQN

Amel_CG42267l KVRAGSSSPESALKEAKTRRD------DEDNRDPARVRSGPDSPDGS

Nvit_CG42267l KLRGSSASPEDVSKDK------EEASRD------LDSPDGS

Dmel_CG34145 PNLGSTAGGGAANGGAGSNAGSGAAGSGAGGAGGASSAVGNPAMLGANQNFPGIVNQAQH

Aaeg_CG34145l ------

Tcas_CG34145l ---QSTLGVNHVN------NTNFP----LHQN

Amel_CG34145l ---STVTSGTGAG------STAQQ

Nvit_CG34145l VPVANAATANANN------PAAQQ

Dmel_run LHHQSE----PVH------VSPASS

Aaeg_run ITLSSSDFISSSR------LSPASS

Tcas_run ------ISPVSN

Amel_run ------TPPKSP

Nvit_run ------TPPRSP

Mmus_Runt1 YSPPVTSGIGIG------MSAMSSASR

Mmus_Runt2 YTPPVTSGMSLG------MSATT---H

Mmus_Runt3 YS-ATPSGTSLGSL------SVAGMPASSR--F

Nvec_Runt ------

Spur_Runt1 SSTATPVTLTSPPYLPNSP------PYPLYPHLYMSSPSSQTYY

Spur_Runt2 S-TAIPMTPSAN------PIYPHLYMSTPSSQTHY

Blan_Runt SSGPTTTMSMLP------TSLTSP--RY

Dmel_lz YQTAGYGGGN------TGGGSASKSELDYGGSYNQAWSNGYQNYQYGSCLATAQYG

Aaeg_lz ------

Tcas_lz ------

Amel_lz FAGGGTGSGS------PGYHYGPSWTS--GPATPVPVASHNYNPNYQG------YY

Nvit_lz YATATGSPGS------TGYHHYGGWTTAPGPATPVPAPAN-YNPNYQG------YY

Dmel_CG42267 YSDLPWLRNAAAAAAANINPGQENSGIPPLGSNPDHDGVNLIKRCVTLITHNPPDAENAN

Aaeg_CG42267l YQELPWFRQSLFANNNNNNNGTQNNDSKESPESPKSTETNGNKRSITVVSSNGEDEEDCS

Tcas_CG42267l NQLFNNALAAS------LFLNAPLLPPPGQWFYSQFYPHDWAWMNLRHHSLLPRSS

Amel_CG42267l IEVDDRNEQESGKDR-ARSDESVGGQDSPRISPVRSDTEDTTADATAFQEDGTAHLRPSM

Nvit_CG42267l IEVDDSEEPRPKIERRSQSEESLEQDSSPRTSPVRTDTELT------QEETSSRLRPSL

Dmel_CG34145 SAASAYGGGTGVVGGGHGSAAAAAAGCNGSLYPVLPASLLYSQLYTAANQSAHGFHSHTL

Aaeg_CG34145l ------

Tcas_CG34145l MTSSSYP------SSNCNNSIYPVLPASLLYSQLYSAANQS-HNFHS---

Amel_CG34145l DAFSAVS------SLVPDSTTTGQSDPLDPLSSLMSGT-PSQRYQDYVS

Nvit_CG34145l DAFSSVS------SLPDTTTPGVQPDPLDHLSSLMSSTGSAQRYQDYVS

Dmel_run RPSSSSPT------QQHVLLKLNTSIETSSIHEQSASDGDSDDEQIDVV

Aaeg_run RNSTSSPP------STLNQSQIKMSIELNSTNEHSSGE-ESDDEHIDVV

Tcas_run ---SQSPT------AQET------KIHSTVDQSTSE-NSDDEDIDVV

Amel_run SESGSESA------PEEVR-SAFVPIRLNTLPPTTSVVTATSASSPERL

Nvit_run SDSGSESA------PEEVVRSAFAPIRPNSN--NNNIHVPTSSSSPDRL

Mmus_Runt1 YHTYLPPPYP------GSSQAQAGP----FQTGSPSYHLYYGASAGSYQFSMV

Mmus_Runt2 YHTYLPPPYP------GSSQSQSGP----FQTSSTPY-LYYGTSSASYQFPMV

Mmus_Runt3 HHTYLPPPYP------GAPQSQSGP----FQANPAPYHLFYGASSGSYQFSMA

Nvec_Runt ------

Spur_Runt1 DSSHLPMLPSSTRPEDKQEIKHDNRPGEGIP-----HIPHPEMSLTVALNYGSHPQSQIE

Spur_Runt2 GSG--SMLPS-RRPEEKRELEDKEDTSHGISVEIITNLPHPYP--DMPLSYGGHPQNQIE

Blan_Runt LPMSPPGFPTLTSAPGGFVASPNSPPRQLGN----YSPGSPPYGIYHHLYGGSYQYPMLP

Dmel_lz PQAAPPPQPPPPPPVVLCPQLYS-----TVNQNQIHLHLHSSEKLEQYLGTATSADHLTI

Aaeg_lz ------

Tcas_lz ------

Amel_lz N-NPS-QNYISPAPMVLYPQLYS-----TVNQNQIHLHLHGDLSEEQ---VTIAGSNLTI

Nvit_lz HQNPAAQNYIGQAPMVLYPQVFTS----TINQNQIHLHLS----EE-----TLANSNLTI

Dmel_CG42267 P------NASPPVSSTRRSPSPVETIDLDDVSTTSRSASGSSGHGGGVGGGG

Aaeg_CG42267l PRKAIAIDDEEDERRKNSPSVTSTKRSPSP--EVSPDSPCTDSVSSLTVLEGATKLSRSR

Tcas_CG42267l SPLELGHNPNGSTKSEGVDDEVKSD-DGKAKEEKKGKDEGVNLSLHRCRKAAITLVRQK-

Amel_CG42267l ENSNLDQEVLATDGHQVSRRES------KIRLEVGTVDLSTKNK--GQDKENVGQDLTT-

Nvit_CG42267l ENVNELDNSTTQLDHQLDQQPASRGLTVKIRVEG-TVDLSTKTRTHHQDKENVGQDLTTT

Dmel_CG34145 PAHASPNSSVHGELQSVMDHISNV----GVRQQHNIMAGGGVTHPGDLTLIGNCGA----

Aaeg_CG34145l ------

Tcas_CG34145l -LHSHTTQSHHNDLQTVMDQLSTS----NQRQ----MNG---STDLLLSNNGTCAA----

Amel_CG34145l PRSLSTDSSTTESPVHE-EQGFGQ----NY--GNYFPTPGVLPSILYSQLYGNQFQ----

Nvit_CG34145l PRSLSTDSSATESPVHEQDQSFGQ----NCSAGNYFPAPSVFPTILYSQLYNNQFQ----

Dmel_run KSEFD------LDKSLDVAPLRM----RCDLKAPSAMKPLYHESGPGAVANSRQ-----

Aaeg_run KSAFVPILRPAPTSSPPPPAPPSA----RCELKAPSSKKPQFHESAPSEPASPEN-----

Tcas_run KSAFVPIKPANLILQEVQPDSTVQ----DKELKKNELKAP----SSRSIKSLAEE-----

Amel_run P------TKKG--VVVEGS----RNELKAPT------ALIS-RSMSP------

Nvit_run S------NNNNSKRPIEGT----RCELKAPK------ALISHRTKTLG-----

Mmus_Runt1 ---GGERSP-----PRILPPCTN------ASTGAALLNPSLPSQSDVVETEGSHSNSP--

Mmus_Runt2 P--GGDRSP-----SRMVPPCTT------TSNGSTLLNPNLPNQNDGVDADGSHSSSP--

Mmus_Runt3 AAGGGERSP-----TRMLTSCPSG----ASVSAGNLMNPSLG-QADGVEADGSHSNSP--

Nvec_Runt ------

Spur_Runt1 LNTARTMG----QSMG-GMHNSG----VALMQQHRNHSPVTNISPMQQSMGQMNINTT--

Spur_Runt2 LDRERTTG----IQSGPGRMTKA----IVIVNCHALKVSVGLEYSKEKGDGAHNV-----

Blan_Runt --GGSGTPQGPREPSILAPTTNS----QTQNKPHVL-PAQEKEASSQPGSGNHGNRQQDE

Dmel_lz GSLTGSSRSSIEIGQDQYHQQVHHAQQQQQQQQQQQQVHHPQQQQVESAGEVGGSGAGGV

Aaeg_lz ------

Tcas_lz ------

Amel_lz ------SSNRLEIG------VMGE

Nvit_lz ------SSNRLEIG------VLGE

Dmel_CG42267 ------AVGPI

Aaeg_CG42267l ------SVEKL

Tcas_CG42267l ------EEVSS

Amel_CG42267l ------KRKEED------TER--EAV

Nvit_CG42267l ------TRRKDDD------VEISEREA

Dmel_CG34145 ------SVRNIEDGNSN------RQVAALAA

Aaeg_CG34145l ------

Tcas_CG34145l ------AAARQDD------ARLTSNGA

Amel_CG34145l ------NSESPEQ------RAVAD-SC

Nvit_CG34145l ------STEAQEQQH------RALADVSC

Dmel_run ------PSPET

Aaeg_run ------TKLKS

Tcas_run ------KSPNT

Amel_run ------KRSPS

Nvit_run ------KRSPS

Mmus_Runt1 ------TNMPP

Mmus_Runt2 ------TVLNS

Mmus_Runt3 ------TALST

Nvec_Runt ------

Spur_Runt1 ------VGHLPTVD

Spur_Runt2 ------CVD

Blan_Runt ------QMDTQPGGS

Dmel_lz ESAREEDVGDLSQVWRPY--

Aaeg_lz ------

Tcas_lz ------

Amel_lz ENEQRNDV------WRPY--

Nvit_lz ENEQRNDV------WRPY--

Dmel_CG42267 RTRTPKPS---ADVWRPY--

Aaeg_CG42267l NLELQQANSKHSDVWRPY--

Tcas_CG42267l SEKEHLKCNRLGDVWRPY--

Amel_CG42267l VRGRRERSPKPRQVWRPY--

Nvit_CG42267l IRPRRERSPKPRQVWRPY--

Dmel_CG34145 HRGHHNPTDNGGSVWRPY--

Aaeg_CG34145l ------

Tcas_CG34145l QRGPGQNSD--AVVWRPY--

Amel_CG34145l SVR-QEEVRPDNNVWRPY--

Nvit_CG34145l SVRVQEEVRPDNNVWRPY--

Dmel_run TTKIKSAAVQQKTVWRPY--

Aaeg_run PNLIIKQS--AKTVWRPY--

Tcas_run KIHHQQEITATKSVWRPY--

Amel_run PTKIS-SPPPAKPVWRPY--

Nvit_run PTKIQPTTPAVKTVWRPYPC

Mmus_Runt1 -ARLEEAV------WRPY--

Mmus_Runt2 SGRMDESV------WRPY--

Mmus_Runt3 PGRMDEAV------WRPY--

Nvec_Runt ------

Spur_Runt1 DSRKEDV------WRPY---

Spur_Runt2 ESNNQGS------YYL----

Blan_Runt PEKRETV------WRPY---

142 amino acid sites alignment including Runx domain, in fasta format

>Dmel_lz

HQDLLWMERLVQKRQQEHPGELVRTSNPYFLCSALPAHWRSNKTLPMAFKVVALAEVGDGTYVTIRAGNDENCCAELRNF

TTQMKNDVAKFNDLRFVGRSGRGKSFTLTITVATSPPQVATYAKAIKVTVDGPREPRSKTSP

>Aaeg_lz

--DLWWIERMVMEAQQEYPGELVRTGSPYFLCSALPNHWRSNKTLPSAFKVVSLGDVCDGTMVTIRAGNDENFCGELRNC

TAVMRNQVAKFNDLRFVGRSGRGKSFTLSITVSTTPPQVTTYTKAIKVTVDGPREPRSKTSE

>Tcas_lz

--DIWWTEHLIHEIQTEHPGELVRTGSPYFLCSALPTHWRSNKTLPVAFKVVALGDIGDGTIVTVRAGNDENCCAELRNS

TAVMKNQVAKFNDLRFVGRSGRGKSFSITITVSTTPPQVATYTKAIKVTVDGPREPRSKTSE

>Amel_lz

--ELWWTERLVGEAQAEHPGELVRTGSPYFLCSQLPTHWRSNKTLPVAFKVVALGEVVDGTLVTVRAGNDENCCAELRNS

TTLMKNQVAKFNDLRFVGRSGRGKSFSITITVSTTPPQVATYTRAIKVTVDGPREPRSKTRQ

>Nvit_lz

--ELWWTERLVGEAQAEHPGELVRTGSPYFLCSQLPTHWRSNKTLPAAFKVVALGEIGDGTLVTVRAGNDENCCAELRNS

TALMKNQVAKFNDLRFVGRSGRGKSFSITITVSTTPPQVATYTRAIKVTVDGPREPRSKTNA

>Dmel_CG42267

LTPENLIERTVDVLLAEHPGELVKTGSPHVVCTTLPTHWRSNKTLPIAFKVLALGEVMDGTIVTIRAGNDENFCGELRNC

TAVMKNQVAKFNDLRFVGRSGRGKSFTLTIVISTNPIQIATYTKAIKVTVDGPREPRSKVRH

>Aaeg_CG42267l

LTAESLAERTIEGLLADHPGELIRTGSPHVVCTVLPNHWRSNKTLPVAFKVVALGDVGDGTMVTVMAGNDENFCGELRNC

TAIMKNQVAKFNDLRFVGRSGRG------

>Tcas_CG42267l

LTAEMLAERTIDGLLAEHPGELVRTGSPHVVCTVLPPHWRSNKTLPVAFKVVALGDVGDGTVVTVRAGNDENYCAELRNC

TAVMKNQVAKFNDLRFVGRSGRGKSFTLSIIVSTTPPQIATYNKAIKVTVDGPREPRSKTRQ

>Amel_CG42267l

LTADLLTERTLDGLFAEHPGELVRTGSPHVVCTVLPAHWRSNKTLPVAFKVVALGEVGDGTLVTVRAGNDENCCAELRNS

TAVMKNQVAKFNDLRFVGRSGRGKSFTLTIMLQTSPPQVATLSKAIKVTVDGPREPRSKTNP

>Nvit_CG42267l

LTADLLAERTLDGLLAEHPGELVRTGSPHVVCTVLPSHWRSNKTLPVAFKVVALGEVGDGTLVTVRAGNDENCCAELRNS

TALMKNQVAKFNDLRFVGRSGRGKSFTLTIMLQTSPPQIATLSKAIKVTVDGPREPRSKTRH

>Dmel_CG34145

MTSDILAERTLGDFLTEHPGELIRTSSPLFVCTVLPPHWRSNKTLPVAFKVVSLGDIMDGTMVTVRAGNDENYCAELRNC

TAVMKNQVAKFNDLRFVGRSGRGKSFTLTITVSTNPPHIATYNKAIKVTVDGPREPRSKTRQ

>Aaeg_CG34145l

MTSDILAERTLGDFLSEHPGELIRTGSPLFVCTVLPPHWRSNKTLPVAFKVVALGDVGDGTMVTVRAGNDENYCAELRNC

TAVMKNQVAKFNDLRFVGRSGRGKSFTLTITISTSPPQVATYNKAIKVTVDGPREPRSKTRQ

>Tcas_CG34145l

MTSDILAERTLNDFLSEHPGELIRTGSPLFVCTVLPPHWRSNKTLPVAFKVVALGDVGDGTVVTVKAGNDENYCAELRNS

TAVMKNQVAKFNDLRFVGRSGRGKSFTLTIMVSTSPPQVATYNKAIKVTVDGPREPRSKTSS

>Amel_CG34145l

MTSDILAERTLGDFVSEHPGELVRTGSPHLVCTVLPAHWRSNKTLPVAFKVVALGEVGDGTLVTVRAGNDENCCAELRNS

TALMKNQVAKFNDLRFVGRSGRGKSFTLTITVSTTPPQVATYTKAIKVTVDGPREPRSKTSE

>Nvit_CG34145l

MTSDIFAERTLGDYMSEHPGELVRTGSPHLVCTVLPAHWRSNKTLPVAFKVVALGEVGDGTVVTVRAGNDENCCAELRNS

TALMKNQVAKFNDLRFVGRSGRGKSFTLTITVSTTPPQVATYAKAIKVTVDGPREPRSKTRQ

>Dmel_run

PSSMTDMFASLHEMLQEYHGELAQTGSPSILCSALPNHWRSNKSLPGAFKVIALDDVPDGTLVSIKCGNDENYCGELRNC

TTTMKNQVAKFNDLRFVGRSGRGKSFTLTITIATYPVQIASYSKAIKVTVDGPREPRSKQSY

>Aaeg_run

---MADMFSNLHDMLQEYHGELVQTGSPAVLCSALPTHWRSNKSLPCAFKVIALDDIQDGTVVTIKAGNDENCHAELRNC

TAIMKNQVAKFNDLRFVGRSGRGKSFSITITISTYPCQIATYTKAIKVTVDGPREPRSKQNF

>Tcas_run

-PSIMDMYASLQETLQEYHGELVQTGSPAVLCSVLPSHWRSNKSLPIAFKVVALDEVRDGTTVTLKAGNDENYCAELRNC

TAVMKNQVAKFNDLRFVGRSGRGKSFTLTITISSEPYQIATYNKAIKVTVDGPREPRTKSNY

>Amel_run

-LGMD--INAMHETLQACHGDLVRTGSPAILCSALPSHWRSNKSLPVAFKVVALDDVSDGTLVTIRAGNDENCCGELRNC

TAVMKNQVAKFNDLRFVGRSGRGKSFSLTIQISTVPFQVATYTKAIKVTVDGPREPRSKSNY

>Nvit_run

-IGMENHFSAIHDALRACHGELVQTGSPAILCSPLPSHWRSNKSLPVAFKVVALDEVSDGTLVTISAGNDENFCGELRNC

TAVMKNQVAKFNDLRFVGRSGRGKSFSLTIQISSVPFQVATYTKAIKVTVDGPREPRSKSNY

>Mmus_Runt1

KLRS--GDRSMVEVLADHPGELVRTDSPNFLCSVLPTHWRCNKTLPIAFKVVALGDVPDGTLVTVMAGNDENYSAELRNA

TAAMKNQVARFNDLRFVGRSGRGKSFTLTITVFTNPPQVATYHRAIKITVDGPREPRRHRQK

>Mmus_Runt2

RLRPPHDNRTMVEIIADHPAELVRTDSPNFLCSVLPSHWRCNKTLPVAFKVVALGEVPDGTVVTVMAGNDENYSAELRNA

SAVMKNQVARFNDLRFVGRSGRGKSFTLTITVFTNPPQVATYHRAIKVTVDGPREPRRHRQK

>Mmus_Runt3

RTRP--EVRSMVDVLADHAGELVRTDSPNFLCSVLPSHWRCNKTLPVAFKVVALGDVPDGTVVTVMAGNDENYSAELRNA

SAVMKNQVARFNDLRFVGRSGRGKSFTLTITVFTNPTQVATYHRAIKVTVDGPREPRRHRQK

>Nvec_Runt

KIKG---ERSLVEALAEYPGELVKTDSPNFVCSVLPSHWRCNKTLPVAFKVVSLGDIPDGVIVSIAAGNDENFAAELRNA

TAVMKNQVARFNDLRFVGRSGRGKTFSLTITVKTEPPQVATYCRAIKVTVDGP------

>Spur_Runt1

---FKGGERSIVDALSEYPGELVKTESPNFACSVLPNHWRCNKSLPVAFKVVSLGETKDGTMVTIAAGNDENYCAELKNN

TAVMKNQVARFNDLRFVGRSGRGKSFTLSIFIYTNPPQIATYNRAIKVTVDGPREPRRPKPK

>Spur_Runt2

---CKGGERSLVDALSDYPGELVKTESPNFICSLLPPHWRSNKSLPVAFKVVSLGETKDGTVVTIGAGNDENCCAELKNN

IAVMKNQVARFNDLRFVGKSGRGKSFTLSIFVYTNPPQIATCNRAIKVTVDGPRPRRRPKSK

>Blan_Runt

HFKG---DRGLVDALADHPGELVRTDSPNFVCSVLPSHWRCNKTLPVPFKVVALGDIPDGTLVTVMAGNDENYSAELRNN

QAVMKNQVARFNDLRFVGRSGRGKSFTLTITVFTSPPQVATYHRAIKVTVDGPREPRRHRQK