Table S3.Primers designed for SNPdiscovery in Concholepas concholepas

Contig / Primers Name / Primer sequence / BlastX result
20130 / SNP1_CC_20130_F / GGTCACTGCAGGTTGTGGGCA / Calmodulin
SNP1_CC_20130_R / CCCCTACGGGAAGTACCAGAGACA
20350 / SNP1_CC_20350_F / GTACCCCACGCACGCCTGTC / Phenylalanine hydroxilase
SNP1_CC_20350_R / GCGTGGGATCGTGTCGGCAA
20527 / SNP1_CC_20527_F / CCTCCAAAGGCAGGTCGTCGT / Ferritin
SNP1_CC_20527_R / CTGGTCTCGTTCTCTTCTCAAAGCTG
3462 / SNP1_CC_3462_F / CTGACACGGCACGTGACCCC / Catalasa
SNP1_CC_3462_R / CGCGTGGGACCACACCTTGG
SNP_CC_3462_F(2) / GTTCTCTGACCGCGGCACCC
SNP_CC_3462_R(2) / CCAGGAGGGAGGCTGACCGT
3748 / SNP1_CC_3748_F / ACAACCGCTGGGGTGAGGGT / Oxidasa peroxidasa
SNP1_CC_3748_R / AGCGAGGAGGTCTGCCCGAG
SNP_CC_3748_R(2) / ACGCCTCGTCTACCGGGTCC
4219 / SNP1_CC_4219_F / GGCCAGCTGGGTGGCAGAAG / Map-kinase Int. Ser. Prot.
SNP1_CC_4219_R / GCACACCGGGATCTGAAGCCA
SNP_CC_ch1_4219F / CAGCGGAGTAGCGCTTGCGA
SNP_CC_ch1_4219R / CGGCCGAGTGGAGCAGCATC
SNP_CC_ch2_4219F / CGCCCACCCAAGCATCCACA
SNP_CC_ch2_4219R / TGGCACCAAGACCCCAGAGC
4293 / SNP1_CC_4293_F / CGAGCAGCAACAAAGTTCACCAATCCT / SOD CuZn
SNP1_CC_4293_R / TGGCAATGCTGGACCACGCC
SNP2_CC_4293_F / GGCGTGGTCCAGCATTGCCA
SNP2_CC_4293_R / GTGCCAGCAGCCGCGGTAAT
SNP_CC_ch1_4293F / CAGCAACAAAGTTCACCAATCC
SNP_CC_ch1_4293R / ACTAGGAATTCTTGTTGCAGGT
5409 / SNP1_CC_5409_F / TGCGGTGCTTTTCAGTCCATTTCC / HSP70
SNP1_CC_5409_R / GGCCAAGATCCACTCCACGGG
8944 / SNP1_CC_8944_F / TGGCCCCTGAGGAGGTCAGC / HSP70
SNP1_CC_8944_R / CCTGGCCCTGGTGATGCGTG
SNP_CC_ch_8944F / ACGGCAGAGGCCTACCTGGG
SNP_CC_ch_8944R / GGAGGCCTGCAATGGCTCCG
13632 / SNP1_CC_13632_F / AGCGTGATGCTTCAGTCCCCA / PGRP
SNP1_CC_13632_R / GCGGGGGACCAGTCAATCGG
SNP_CC_13632_F(2) / AAGCTCCACAGACTCATTTTTGGATGA
SNP_CC_13632_R(2) / GAGGCAGAGTATGGCCTGCG
3531 / SNP_CC_3531_F / CGCGGCAGTAAACAGGGTGC / Prohibitin
SNP_CC_3531_R / GCGTCAGCAGGTGTCGCTGT
13621 / SNP1_CC_13621_F / CCCCGAACACATCGTGCAGACC / Chorion peroxidase
SNP1_CC_13621_R / GCAGTGCGATACACCTGTGTGCT
SNP2_CC_13621_F / CGGCAAGGGAGGTCAATATCTGC
SNP2_CC_13621_R / CTGCATCAGCAGGAAGGTGAAGT
SNP3_CC_13621_F / GCGACCGATTCTACTTCGAGCAC
SNP3_CC_13621_R / GTGGAGCACAGCACTTTAGCCA
13822 / SNP1_CC_13822_F / GCCCACATCAGCCCACACCA / Oxidase peroxidase
SNP1_CC_13822_R / AAGACGGTGCAGCAGCGAGG
Contig / Primers Name / Primer sequence / BlastX result
13936 / SNP1_CC_13936_F / GGTGACGTTCCAGCCCCAAT / Peroxirredoxin
SNP1_CC_13936_R / TTCCAAAAGAGAACGGCATGGGA
17048 / SNP1_CC_17048_F / TACCTCAACGGCATCGGCGG / Serine protease
SNP1_CC_17048_R / GGCAGGCGTCCTTTCCACCC
19906 / SNP1_CC_19906_F / GGAGGAGGAACTGAACAAAACCAAGCC / HSP90
SNP1_CC_19906_R / GGTCAGGGACTTGTAGAACTCGGCA
19966 / SNP1_CC_19966_F / TACCCATCCGCTCTGCCCCC / Serine Thr. Prot. Kinase
SNP1_CC_19966_R / CCAGAGGTGCAGCGCCAGAC
13615 / SNP_13615_F / AGCCACTGTCTACCACCCCC / Cathepsin
SNP_13615_R / GGGAGGCTGGCCTGCCAAAG
SNP_CC_13615_F(2) / ATGATGGTGATGTCACGTGCAACTT
SNP_CC_13615_R(2) / GCCGGCAAGTTTGGCAATCG
13616 / SNP_13616_F / AGGTGATGTGCGTGGCTGGC / Chitin deacetylase-like isoform e
SNP_13616_R / CGCAGGAAGGCGGTGACGTT
SNP_CC_13616_F(2) / GAGTCTGAGGTGCGAGGGCT
SNP_CC_13616_R(2) / GCAGCTGTCGGTCATGGAGC
13705 / SNP_13705_F / CGCCTGCTGTGCTCGTGTGT / Heat shock factor binding protein 1
SNP_13705_R / TGCGTTGATCACAAGCACACTT
13807 / SNP_13807_F / AGGGCTGACACGGAGAGGCT / Uiquitin
SNP_13807_R / GCAGCGTCTGATCTTTGCTGGC
14348 / SNP_14348_F / CAGCGAGCCGTGCGTACGAT / Inhibitor of apoptosis protein
SNP_14348_R / AGCGACACGGTTGCCGACTG
HRM_CC_14348_F / GCTTGCTGGAGAAGACCGGA
HRM_CC_14348_R / AGGGAGAGAACGTCCACAACG
19900 / SNP_19900_F / GCTGAAGACCATTGCCACTGAGGG / A bovine heavy chain protein
SNP_19900_R / GCCTGTCACTCACTGTCGTATTT
SNP_CC_19900_F(2) / GGTGCCATCGTGGATGTGCC
SNP_CC_19900_R(2) / CACGCTGACCACGTCCGATG
3445 / SNP_3445_F / GGTCATGGCGTGGACGGTGG / Glyceraldehyde-3-phosphate dehydrogenase
SNP_3445_R / TCGGTCGCCTGGTGCTGAGA
SNP_CC_3445_F(2) / TGGTGCAGGAGGCGTTGCTG
3548 / SNP_3548_F / ACAGCGTGGACTGCGTGTGG / Caveolin 1
SNP_3548_R / CTGGGCGCAGCCACAGTTGA
3549 / SNP_3549_F / GCCGCTAGGGGAGGGCTTCT / Phospate transporte
SNP_3549_R / TGTCGGCCACGCACAGCATT
3998 / SNP_3998_F / CAAAAAGGAGATGTGAACAATTTAGTC / Peptidylprolyl isomerase roc4
SNP_3998_R / TGAAGGACATGCTGGTGCAG
4010 / SNP_4010_F / GCAGGCGGGGCTGATGACTG / N/A
SNP_4010_R / TGTTGGCGAGGGAGAGCACCA
SNP_CC_4010_F(2) / GCATCATCCCCACAGCCCTGC
4116 / SNP_4116_F / TCACGATGCATCCCCAACGCA / Ubiquitin-conjugating enzyme
SNP_4116_R / ATGAACGCAGGGTTCGGGCG
HRM_CC_4116_F / CACGATGCATCCCCAACGCA
HRM_CC_4116_R / ACAGCGATGAACAGCATCTCAAGAA
Contig / Primers Name / Primer sequence / BlastX result
8740 / SNP_8740_F / TTGTGGCTGAGCAGGTGGCG / Inhibitor of apoptosis protein
SNP_8740_R / GCGCGCCTGTCCCCTAGTTG
HRM_CC_8740_F / GGTGGGGAGGAAATCCCATCTGC
HRM_CC_8740_R / CTCACTTCTGCTCAGCTGGACG
8764 / SNP_8764_F / GCCTTGGCTGGGGTGACAGC / Tep-1
SNP_8764_R / CACCACGTGACCGCTTGGCA
10337 / SNP_CC_10337_F / CAGCCTCCCTGACGCCTTGG / N/A
SNP_CC_10337_R / TCCGGCATGGCAGAGTCACC
SNP_CC_10337_R(2) / ACAGCCTCCGATGTAGCAGCA
10431 / SNP_CC_10431_F / GCAGGACGGTGGCCATGGAA / Phosphatidylinositol glycan anchor class partial
SNP_CC_10431-R / TCCGGTCACCTGAATCTCTCAGCA
SNP_CC_10431_R(2) / ACCACCAAGATCTGCGCCCG
11588 / SNP_CC_11588_F / ATGTAAAAAGGCCGATTAACAAGGAGA / N/A
SNP_CC_11588_R / TCATTGCTTGCATTGGGTGCAG
SNP_CC_11588_R(2) / GCCTATTAGTGTGCAGTAAACAGGGA
12687 / SNP_CC_12687_F / TGCTCCCTTCTCTGCTGTCTCC / N/A
SNP_CC_12687_R / AACCACCAACACCGCCTACA
13753 / SNP_CC_13753_F / TGTCCAGTTTCCCCAGGTCTCTCT / Ribosomal protein 18S
SNP_CC_13753_R / CACCGTGATGGGCAACCCCC
13860 / SNP_CC_13860_F / CGCTGGTGACCTCCCGCTTC / Ribosomal protein l22
SNP_CC_13860_R / GCGCAGCTCGAAGGCCTCTT
SNP_CC_13860_F(2) / TGTCCGGAAAGGGGAAGAAAAAGA
SNP_CC_13860_R(2) / CAGCCAGTCACGCAGGTTGT
14644 / SNP_CC_14644_F / GGCCTCCACCGGGCATCTTC / Cytochrome b
SNP_CC_14644_R / CGGAGAAAAGGTACCCTTCCATTCTT
18164 / SNP_CC_18164_F / CCCCATCCCCATCCCGTCCA / Protein
SNP_CC_18164_R / CCCTCCACCCGACCCGTCTT
SNP_CC_18164_F(2) / CCAAGTTTGACGATCGATTTGCACG
SNP_CC_18164_R(2) / CGAAGTGAAAGCGAGGGCCG
20569 / SNP_CC_20569_F / AGCACTGACAGAACACACACCTGC / Protein (oxidation reduction)
SNP_CC_20569_R / CTTCAGAGCGGATCGCCCCC
SNP_CC_20569_F(2) / CCTTATAAGACAAACAAGAGGTGTTCG
SNP_CC_20569_R(2) / GCTTAATTCCAGAAGCCAGGGG
3651 / SNP_CC_3651_F / CCCCGCACTTTATTTGTTTGGTTGGG / 60S ribosomal protein l8
SNP_CC_3651_R / CCGGCCGTGCCTACCACAAG
3717 / SNP_CC_3717_F / GCAGAGGTCGCAGGCAGGAC / N/A
SNP_CC_3717_R / CGGGCCTAGTGGTGCGTGGT
3857 / SNP_CC_3857_F / GCCTGCACTCGCCCACTGTC / ribosomal protein S11
SNP_CC_3857_R / CGACACCATGGCGGACGCTC
HRM_CC_3857_F / TGGACAGACATGTTCTTGTGCCGC
HRM_CC_3857_R / TGTCATCCGGAGGGAGTACCTTCA
7314 / SNP_CC_7314_F / GTTGGAGGGGACTTGCCAAGGG / Protein (oxidoreducatse activity)
SNP_CC_7314_R / ACGCGAGGTTTCCGTCCTCG
HRM_CC_7314_F / AGGTGTAGCATAGGTGGGAGC
HRM_CC_7314_R / CGCTCGACTGAATAAGTAAAGAAACG
Contig / Primers Name / Primer sequence / BlastX result
8730 / SNP_CC_8730_F / AGACGGTGCTTGCCATGATGCT / N/A
SNP_CC_8730_R / TGGAACACCCAGAGCCCACCA
8808 / SNP_CC_8808_F / GGCTCGAAGCAGGCGTTGGT / Tubulin alpha-1 chain
SNP_CC_8808_R / CCCCGCTCCTCAGGTGTCCA
8881 / SNP_CC_8881_F / ACTGGCGTGGACGAGCAAGG / Ribosomal protein l27
SNP_CC_8881_R / GCCCTTCAGGGCAGGGTCAC
8924 / SNP_CC_8924_F / GCCTTGCGACCATACTCCCCC / Hypothetical protein (sodium ion transport)
SNP_CC_8924_R / TCTCCGGATCGGCTTCCCGC
SNP_CC_8924_F(2) / ACTCGGACCCCCAAACCAACG
9666 / SNP_CC_9666_F / TCGTCAAACTTGGGTATAGGGGCGA / N/A
SNP_CC_9666_R / AGCAGGCTCTGTGCGTAGGGT
988 / SNP_CC_988_F / AGAGGAGACAGAGGAGGGTGACT / N/A
SNP_CC_988_R / TGAGGTACAACAAACAGGGTGAACCA
12162 / SNP_CC_12162_F / AAGCGCTCTTTCCCGGAGGG / Predicted protein
SNP_CC_12162_R / CCTGTACAGACCCTCGCGCT
19694 / SNP_CC_19694_F / GCTGTCACTCAATTTTGGCGTGC / Ribosomal protein 40S
SNP_CC_19694_R / TTGCTAGACGCAGCAGAGAGTCA
17850 / SNP_CC_17850_F / GGCTGTCACTCATTTCCCTTGGCG / protein dpy-30 homolog
SNP_CC_17850_R / TCCAGGTAAGCCCTGGTTGGCA
26196 / SNP_CC_25196_F / CCCCTGGTTGGTCCGTTCCG
SNP_CC_ch_25196_F / GTCCATTGTGCACGGACGGGA
3461 / SNP_CC_25196_R / AGCGATACCCACTGCCCATTCCT
SNP_CC_3461_F / ACATTTTCCACGAGTTTGGA
SNP_CC_3461_R / CTACCGACGACGTAAAAGTT
SNP_CC_ch_3461_R / AAACGAGGTAGGAGTCTTGT
14637 / SNP1 _CC_14637_F / CGGACCTTGGCTGTCACTCAAC
SNP1_CC_14637_R / GGCACTGGCAGGTCTGCACTT
SNP2_CC_14637_F / AGTGCAGACCTGCCAGTGCC
SNP2_CC_14637_R / GCTAGAGCTCCGAGGTTCACCA
4402 / SNP_CC_4402_F / GCGACCTTGGCTGTCACTCA
SNP_CC_4402_R / GGGGGTTCCCGGTAAAGGGC
SNP_CC_ch_4402_R / ACCAGGGTGTAAAGTTTGACTGC
10059 / SNP_CC_10059_F / GGGCCAGGGCTTCATGTCCG / myosin essential light chain
SNP_CC_10059_R / GCACCAACTGCTGATCTCTGCTGC
10293 / SNP_CC_10293_F / ACCTCTACTTGTGAAGAAGACCCCC / hypothetical protein
SNP_CC_10293_R / AACTGTGTGAGGGAGGCCCA
11691 / SNP_CC_11691_F / TGGCTGTCACTCACACACTTTCCA / PREDICTED: family with sequence similarity 98, member B
SNP_CC_11619_R / ACCTGGTGATGTGATTGCCTTGGG
1271 / SNP_CC_ 1271_F / CGGACCTTGGCTGTCACTCAC / N/A
SNP_CC_1271_R / AGCATATATGTTTGCAGCGAAAAGCC
14176 / SNP_CC_14176_F / GGGCTGGTTGATTCGGCAGGT / hypothetical protein SERLADRAFT_353288
SNP_CC_14176_R / GGCTCGCTTGACTGGAGCCG
15551 / SNP_CC_15551_F / GGGCGTCGTGCTTGTCACCA / ribosomal protein L28
Contig / Primers Name / Primer sequence / BlastX result
SNP_CC_15551_R / TTTTCGGGGCACCACAGGGC
15634 / SNP_CC_15634_F / ACGTACTGCGTAGAGTTGCAGCAT / translationally-controlled tumor protein-like protein
SNP_CC_15634_R / ACACATCTGCCGTCAGGCCG
15800 / SNP_CC_15800_F / GGAAGACAATGTGGTCAGGCGGG / N/A
SNP_CC_15800_R / TGGCATCAGGGGAGTAGGGGG
15959 / SNP_CC_15959_F / ACCAGGGGATAAGGATCGTGCC / ThiC family protein
SNP_CC_15959_R / CCCTCCCTGGCTTCACTCCAC
22576 / SNP_CC_22576_F / CGTGAACTCTTCTCGAGTCTCCCGT / Chorion peroxidase
SNP_CC_22576_R / ATGGGTGACAGCCCGCAACA
23743 / SNP_CC_23743_F / GGAGCGGCTTAGTGCAAAGGCA / leucine aminopeptidase
SNP_CC_23743_R / TCAGCGCGAAAGTGGCTCCC
4359 / SNP_CC_4359_F / AGGGTGACTCTGCCATGCCG / N/A
SNP_CC_4359_R / ACAGAAAGCAGGAAGTTGGCATCTGA
5514 / SNP_CC_5514_F / CTGACACAAGCACAGGGCCG / glutamate dehydrogenase 1
SNP_CC_5514_R / AGCTTTTTGCACTTGGCGAGACC
5717 / SNP_CC_5717_F / CGTGATGGGCAACCCCCGTC / PREDICTED: similar to putative ribosomal protein S18
SNP_CC_5717_R / CTCTGCGGCCGGTGGTCTTG
5754 / SNP_CC_5754_F / AGCGCATCAAGGTGGACGGC / ribosomal protein L22
SNP_CC_5754_R / GCGCAGCTCGAAGGCCTCTT
6083 / SNP_CC_6083_F / TGCTGCTGCAATTCTCGACTGGA / N/A
SNP_CC_6083_R / GCCAAAACAGCCATCCGCCC
6203 / SNP_CC_6203_F / TCGCACTGCTGATGGTTCCTGC / hypothetical protein DAPPUDRAFT_320790
SNP_CC_6203_R / CTGCCCCCACTGCATCAGCA
8208 / SNP_CC_8208_F / ACGGGCGTAGTTGTTGGCGG / PREDICTED: GL12416-like
SNP_CC_8208_R / CCAGCCTGACGGCCAGATGC
8294 / SNP_CC_8294_F / AGCCTCGTTGTCGATGCAGTACG / Tubulin beta chain]
SNP_CC_8294_R / CTGGGTGGTGGCACTGGCTC
9665 / SNP_CC_9665_F / GGCATCGCAGCATACCTGGGG / GF22422
SNP_CC_9665_R / TCGATGCCCAGCCCAAAGCC
9671 / SNP_CC_9671_F / GCGTCTTCTCGACCAACGGC / PREDICTED: similar to Ac1147
SNP_CC_9671_R / CTGGCCCGAAAGGGTTGGGA
9861 / SNP_CC_9861_F / ACCAACGCCTGCTTCGAGCC / PREDICTED: tubulin alpha-3 chain
SNP_CC_9861_R / TCACCTCCGGGCACCACTGT
11096 / SNP_CC_11096_F / CAGACAGAAAGACACACACA / unknown
SNP_CC_11096_R / CTGTCACTCCCCTATGTCTT
14912 / SNP_CC_14912_F / AAAGGCCGATTAACAAGGAG / pugilistDominant
SNP_CC_14912_R / GTTGCCTATTAGTGTGCAGT
14921 / SNP_CC_14921_F / ACACCACCAACAAAAGTTCA / N/A
SNP_CC_14921_R / TGGCTGTCACTCAAGTCTAT
15081 / SNP_CC_15081_F / TTTCCACGTTTTGCATTGAC / N/A
SNP_CC_15081_R / TAGTGTGCAGTAAACAGGGA
9979 / SNP_CC_9979_F / GTTGTTCAATTCATCGGCTG / predicted protein
SNP_CC_9979_R / TGAAAACCCACGACTCAAAA