Supplementary Information

Kinetic modeling and community dynamics of microaerophilic treatment of textile dyes containing effluent by consortium VIE6

Binal Shaha, Ami Patela, Datta Madamwara*

a Environmental Genomics and Proteomics Lab, BRD School of Biosciences, Satellite Campus, Vadtal Road, P. Box # 39, Sardar Patel University, Vallabh Vidyanagar – 388 120, India

*Corresponding author:

Tel: +91 2692 229380

Fax: +91 2692 236475

E-mail address: (D. Madamwar)

(B. Shah)

Figure S1: Effect of HRT…..

Figure S2: Effect of dye concentration…..

Figure S3: Effect of salt concentration…..

Figure S4: Determination of kinetic constants….

Figure S5: FTIR spectral analysis…..

Legends to Supplementary Figures

Figure S1: Effect of HRT over various parameters (phosphate, sulphate, phenolic, alkalinity, pH, Total Solids (TS), Biochemical Oxygen Demand (BOD), Total Organic Carbon (TOC) and Total Nitrogen (TN)) after UMFB operation at 37 °C under steady state conditions.

Figure S2: Effect of dye concentration over various parameters (phosphate, sulphate, phenolic, alkalinity, pH, Total Solids (TS), Biochemical Oxygen Demand (BOD), Total Organic Carbon (TOC) and Total Nitrogen (TN)) after UMFB operation at 37 °C under steady state conditions.

Figure S3: Effect of salt concentration over various parameters (phosphate, sulphate, phenolic, alkalinity, pH, Total Solids (TS), Biochemical Oxygen Demand (BOD), Total Organic Carbon (TOC) and Total Nitrogen (TN)) after UMFB operation at 37 °C under steady state conditions.

Figure S4 : Determination of kinetic constants from modified Stover-Kincannon and Grau second order muticomponent substrate removal model for (a,c) organic matter (COD) and (b,d) textile dyes, respectively.

Figure S5: FTIR spectral analysis of (a) SDM (200 mgL-1) and (b) extracted metabolites from UMFB at 2 d HRT, 37 °C.

Table S1: Specifications of primers used in study for qPCR assay

Stain / Primer Name / Sequence (5'→3') / Tm (°C) / GC (%) / Amplicon Size (bp)
Bacillus sp. DMB1 / DMB1F / ACT AGC GAT TCC AGC TTC AC / 57.3 / 50.0 / 100
DMB1R / GTG CTA CAA TGG ACA GAA CAA AG / 58.9 / 43.5
Staphylococcus sp. DMB2 / DMB2F / ACC ACC TGT CAC TTT GTC C / 52.6 / 52.6 / 85
DMB2R / CGC GAA GAA CCT TAC CAA ATC / 57.9 / 47.6
Escherichia sp. DMB3 / DMB3F / GGT GGA GCA TGT GGT TTA ATT C / 58.4 / 45.5 / 92
DMB3R / CGA AGG CAC ATT CTC ATC TC / 57.9 / 47.6
Enterococcus sp. DMB4 / DMB4F / GTG CTG ATC CGC GAT TAC TA / 57.3 / 50.0 / 102
DMB4R / GTC GCG AGG CTA AGC TAA T / 56.7 / 52.6
Pseudomonas sp. DMB5 / DMB5F / CAA CTA GCC GTT GGA ATC CTT G / 60.3 / 50.0 / 84
DMB5R / TAA CCT TGC GGC CGT ACT / 56.0 / 55.6