Supplemental Table 2:Alleles in microsatellite loci in single cells of 4 HMs with two cell populations

HM142 / D6S105 / D6S2443 / Interpretation
Paternal / 117/119 / 175
Maternal / 121/123 / 193/197
HM, tissue / 117/123 + 117 / 175/197 + 175 / P1M + P1P1
HM, single cells (9)a
(1) / 117/123 / 175/197 / P1M
123 / 165/197 / Contamination with alien DNA
HM173
Paternal / 117/123 / 165/175
Maternal / 117/121 / 165/167
HM, tissue / 121/123 + 117 / 165/175 + 165 / P1M + P1P2
HM, single cells (1) / 123 / 165/175 / Possible P1M, drop-out of one allele
(1) / 121 / 165 / Possible P1M, drop-out of two alleles
(1) / - b / 165 / Possible P1P2, no PRC product in D6S105
(5) / 117/121/123 / 165/175 / Possible P1M or P1P2, contamination with DNA from HM tissue
(2) / 117/121/123 / 165 / Possible P1M or P1P2, contamination with DNA from HM tissue, and drop-out of one allele
(1) / 117/121 / 165/175 / Possible P1M or P1P2, contamination with DNA from HM tissue, and drop-out of one allele
(3) / 117/123 / 165/175 / Possible P1M or P1P2, contamination with DNA from HM tissue, and drop-out of two alleles
(1) / 117 / 165/167/175 / Contamination with alien DNA
(3) / 117 / 165/167 / Contamination with alien DNA
(1) / 113/115/117 / 165/167/175 / Contamination with alien DNA
(1) / 115/117 / 165/167 / Contamination with alien DNA
(1) / 115/117 / 165 / Contamination with alien DNA
(1) / 117/121/123 / 165/167/175 / Contamination with alien DNA
(3) / - / - / No PRC product
HM539
Paternal / 121 / 165/175
Maternal / 117 / 169/193
HM, tissue / 117/121 + 121 / 165/169 + 165 / P1M + P1P2
HM, single cells (9)
(1)
(2)
(3) / 117/121 / 165/169 / P1M
121 / 165 / P1P2
121 / 165/169 / Possible P1M, drop-out of one allele
- / 165/169 / Possible P1M, no PRC product in D6S105
HM652
Paternal / 117/123 / 167/195
Maternal / 117 / 197
HM, tissue / 117 / 167/197+167 / P1M + P1P2
HM, single cells 13)
(1)
(1)
(1)
(1)
(1) / 117 / 167/197 / P1M
117 / 167 / P1P2
- / 167 / Possible P1P2, no PRC product in D6S105
117 / 167/189/197 / Contamination with alien DNA
117/123 / 167/197 / Contamination with alien DNA
- / - / No PRC product

a) The numbers in the parentheses refer to the number of cells counted with the respective allele combination

b) -: No signal