Supplemental Data. Hotto Et Al. (2015). Plant Cell 10.1105/Tpc.114.134452P. 1 of 20

Supplemental Data. Hotto et al. (2015). Plant Cell 10.1105/tpc.114.134452p. 1 of 20

Supplemental Dataset 1: Sequences and alignmentused for the phylogenetic analysis shown in Supplemental Figure 1 (FASTA format).

>gi|495455085|ref|WP_008179779.1| hypothetical protein [Moorea producens]

MAQTDAQLDPLEEDSHGGGTDSIVLWTEFRFRQFMAKVDQIAKIQQISPASLAYIGDAVYELYIRTHYLLPPRRICDYHNQVVAHVRAESQAKILRSLEPYLTASEKDVLRRGRNAATKTPRRLNREIYQQASSLETLLGYLYLTDTQRLTQLLAHLKLDSP

>gi|517210072|ref|WP_018398890.1| hypothetical protein [filamentous cyanobacterium ESFC-1]

MDRQDGQNSLSSPLRLKAIATDLETSLPGIEQLSPVALAYLGDAVYELYLRSYYLLPPQRISAYHQQVVAQARAERQADHLQALEVDLTAAERDILRRGRNAATRRPQRLSAAVYRQATSLETLIGYLYLTNPDRLNQLLAKLELDSLET

>gi|503086050|ref|WP_013320910.1| ribonuclease III [Cyanothece sp. PCC 7822]

MELKAICSLTAPLLKIGQISPASLAYIGDAVYELYVRTRYLLPPRRLCDYHNQVVAKVRAEQQAAYLQVLEPHLTESEREIVRQGRNAANGRPRRLSPELYQQASSLETLIGYLYLQNPQRLNELLEILEF

>gi|639207193|ref|WP_024545458.1| Mini-ribonuclease 3 [Synechococcus sp. NKBG15041c]

MFVPKISADRLRQFSPASLAYVGDAVYELYCRCYFLLPPRRSADYHDRVVAEVRAETQAQYLTVLEPLLTDLERDIVRRGRNAATGKPRRLSVTLYRQATGFEALIGYLYLTNQSRLEELFAHLPRETPGV

>gi|501592006|ref|WP_012595703.1| MULTISPECIES: ribonuclease III [Cyanothece]

MNLPHSDLNQSLFLSNDSLPNPGVMLESVGLDPSKISSIGQVSPAFLAYLGDAVYELYIRTYYLLPPRRLVDYHNQVVSFVRAETQAALLEYLKPYLTDAEREIVRRGRNAATGKPRRLSPEIYQQATSLETLIGYLYLHNQGRLTQLLEKLNLSQLT

>gi|497231352|ref|WP_009545614.1| MULTISPECIES: ribonuclease III [Cyanothece]

MSQHRPGVNFQVIMEAKDYDNISQLSPAFLAYIGDAVYELYVRTSYLFPPRKVRDYHNQVVAQVRAETQAKALQRLTPYLTTVEQDIVRRGRNAATGCPRRLQPEIYQQATSLETLIGYLYLQDTHRLRELLETLIP

>gi|495549199|ref|WP_008273778.1| hypothetical protein [Cyanothece sp. CCY0110]

MSQHRPGVNFQVIMEAKDYETISQLSPAFLAYIGDAVYELYVRTSYLFPPRKVRDYHNQVVAQVRAETQAKALQRLTPYLTTVEQDIVRRGRNAATGCPRRLQPEIYQQATSLETLIGYLYLQDTHRLHELLETVIL

>gi|506435475|ref|WP_015955192.1| ribonuclease III [Cyanothece sp. PCC 7424]

MTSSKLNPKPKNQSLLSITPQEAVRLKVICISTAPLSQMGQLSPASLAYIGDAVYELYVRSVYLLPPRRLSDYHSQVVAQVRAEQQAAHLQTLEPYLTETEQEIVRQGRNAATGRPRRLSPKLYQQATSLETLIGYLYLQDPQRLTQLLEYLEL

>gi|502718511|ref|WP_012953495.1| hypothetical protein [Candidatus Atelocyanobacterium thalassa]

MIKKCYPDVGFRMFVQTKDHSSIDRLSPAFLAYIGDAVYELYVRTAYLLPPRKLADYHNQVVAQVRAETQAAALQQLQPYLTETEKDIVRRGRNATNSHPRRLKPEVYQQATSLETLIGYLYLQDSQRLNEILRNLV

>gi|504957396|ref|WP_015144498.1| hypothetical protein [Pleurocapsa minor]

MDVQQSKWNPANLVFVPGADVGCEIACTDVDRAYLIGQLSPASLAYIGDAVYELYVRTRYLLPPKRLSDYHDRVVAQVRAESQAACLRVLESYLTDEEREILRRGRNAATGKPRRLSPELHQQATSLETLIGYLYLQNPQRLNELLEHLNLE

>gi|493032906|ref|WP_006102210.1| RNase3 domain protein [Coleofasciculus chthonoplastes]

MEQQEETANSVDNNSALSGTEARFRQLNTAADPQCQIQQISPAALAYLGDAVYELYIRRHYLLPPKRLCDYHDQVVAQVRAESQANTLRSLEPYLTEPELALLRRGRNAATGTSRRLNRKIYQQATSLETLLGYLYLTDPQRLTQLLAYLELDTP

>gi|493557334|ref|WP_006510819.1| hypothetical protein [Xenococcus sp. PCC 7305]

MNLPNPNQDQIESIDTPLGVDVTALASSITVVGDSQLKRLSPVALAYIGDAVYELIVRAKLLLPPKRIADYHSQVVAQVRAEAQATHLQDLEPYLTPAEKEILRRGRNAVTNKPRRLSMKIYQQASSFETLIGYLYLEDSQRLQDLFTKLNL

>gi|505006810|ref|WP_015193912.1| ribonuclease III [Stanieria cyanosphaera]

MSLPKPDRDQPELTTSASVQAEASLLFNQMTTSLVSQVESLSPIALAYIGDAVYELYVRTCYLIPPKRLADYHNQVVAQVRAESQADYLQLLQPYLTFSEQEIVRRGRNAVDKKPRRISAQIYQQATSFETLLGYLYLTNPQRLDELLAKLKINSSKLLT

>gi|501263000|ref|WP_012306018.1| ribonuclease III protein [Synechococcus sp. PCC 7002]

MFVPDITADQLRQLSPASLAYVGDAVYELYSRCYFLLPPCRIADYHGKVVAEVRAETQAQYLTLLEPHLSELERDIVRRGRNAATGKPRRLSLAIYRQATGFEALVGYLYLTNPQRLAELFTCLPQERSGV

>gi|505002871|ref|WP_015189973.1| ribonuclease III [Gloeocapsa sp. PCC 7428]

MNPKEEELLDRSFIIEDELTLKLEQLLPTLAEDLLQLQQASPSALAYLGDAVYELYIRRYYLLPPKRSQVYHRLVVAQVRAEAQASHLRSLTPYLTSTELDIVRRGRNATTKPPKRIEPEIYQQASSLETLVGYLFLTDCQRLAQLLHKLDFEQNQNE

>gi|488881745|ref|WP_002793970.1| Similar to tr|P74327|P74327 [Microcystis aeruginosa]

MESPLEQIRLQVNGEGSPILEGLDRLSPASLAYLGDAVYELYIRTHYLLPPQRLGDYHAQVVGKVRAEQQALDLAQLQPYLTDQEKEILRRGRNAVTGKRRRLTAQVYQQASSLETLFGYLYLQDPSRLHQLLEKLEL

>gi|494522639|ref|WP_007312092.1| cysteinyl-tRNA synthetase-like small protein [Crocosphaera watsonii]

MSPAFLAYIGDAVYELYVRTCYLLPPRKVGVYHNQVVAQVRAETQAKALQKLTPLLTTVEQDIVRRGRNAATGCPRRLKPEIYQQATSLETLIGYLYLRDTERLSELLNILIT

>gi|504986721|ref|WP_015173823.1| ribonuclease III [Geitlerinema sp. PCC 7407]

MNTLDTPSQPLPERPELEIALPWAALGRPCDQSQALTSAEIRRLSPVALAYLGDAVYELFVRTRYLMPPRRIQTYHHEVVDQVRAERQAEYLQSLEPYFTPEELDILKRGRNAVPSGRTKRVSPEIYQQASSLETLMGYLYLTDPARLFQLLSYLPFEAKSSLTAHS

>gi|550282684|ref|WP_022608994.1| Ribonuclease III [Rubidibacter lacunae]

MSAAARDDATPDVVSSRALPTDGDAREFLSVQLDCLRAGFDTDPTSALAAQRLSPAVLAYLGDAVYEVCARSRYLLPPKRLSDYHRLVVSAVRAETQAERLQTLEPYLNADERDILRRGRNAASGRPRRLSPLIYQQATSLETLIGYLFLCDRERLLQVLQWLDLCA

>gi|515490916|ref|WP_016924170.1| hypothetical protein [Prochlorothrix hollandica]

MEADPKPSPLSQSITAIAMDGGQPLLSPQEAQHLSPVLLAYVGDAIYELYVRLHYLLPPQRIQAYHRQVVGQVRAEQQSLHLQTLRQHLTEAESAIVRRGRNAATSAPKRVKVSVYQQATALEALVGYLYLTDPARLKVLWQYLVFCDSP

>B.subtilis

MLEFDTIKDSKQLNGLALAYIGDAIFEVYVRHHLLKQGFTKPNDLHKKSSRIVSAKSQAEILFFLQNQSFFTEEEEAVLKRGRNAKSGTTPKNTDVQTYRYSTAFEALLGYLFLEKKEERLSQLVAEAIQFGTSGRKTNESAT

>C.reinhardtii

MSEMRTYMRRIGGSKAALPRRCSDRRSSLHCTCMLPPPQLTGSAAANPRSIYSPLALAYLGDAVWEVSMAAQAVGERDFSASMPITVSPAGAAAIAAAAATDASGAAASQLLVLTPAEADVLRWGRNAAVGSIPKSVSAGTYKKATAVEVLVAHLYLTAPQRCAQLIQAAVSAVPLQP

> A.lyrata

MVILSASSTVRAALDTQPRLPYNPNAPRKVKKTPNNNSFLPPPSPPPSPGISISVADLLKRPASKELTVDVDDTYMGYETWSPSPPKLEKPRSVFNAASLAFIGDSIFELYARRHFLFPPLSIEDYNDRVRAVVRCEAQYALLKKLVDDDFLTKEERDILRWGKNLGSARTRTRRRAGNAVYNKASSLETLIGYLYLTNGKRLEKIMQKLGFSSDSSTEIVIEEAKPKPSESNLSNFILNEQVVS*

>A.lyrata_2

MAILSASSTIVRAALDTQPKLRYNPNAPRNVKNLSSFVPPLSPSSSSPATNLTTGTNVSVSDLLKRPASK

DVGNGFDDNCIGYDKWFPSPPKVEKPRSVFNAASLAYIGDSIYEQGYQVMIILFFRMQGCQMRIIFSDYS

IKTATMSLCYFLYLSLAVLGYKNIYARRHFLFPPLSIEEYNDRVRAVVRCEAQYALLQKLVDDDFLTKDE

RDVLRWGKNVGSAKTRSTRRAGVAVYNKASSLETLIGYLYLSNGKRLEEMMQKLGFSSGSSTERMIKEAG

KNKPSFK*

>C.papaya

MPVLCTNISPGLRVRTSLDTQRRLPYNPHTPPKPKRSPNDFAPSPAVTLTNNCTDRSGADLLKRRLDQGVANKRDESYMGYEIWLPRPPKVDKPRSVYNAASLAYIGDCIYELYARRHFFFPPLNIEEYNDRVTAVVRCEAQDALLQKLLNDNYLTEEERNVLRWGKNIGSAKTRSRKRAGVAVYNRASSLETLIGFLYLTNVSRLEEVMLKLGFSTGSSTQDILEEGNIVEFNIQASFLLITGWKTAMTATT*

>R.communis

MPTILSTMSSSLRVSVRASWDTQQRLTYNPNAPRKHTKNPTSQPNTLSLPPPTTTSPLSVTVTTQSVSNLLKRNTTPQGVMKKLDDTYLGYERWMPTPPKVEKPRSVYNAATLAYIGDSIYELYARRHFLFPPLSIEEYNDRVMSVVRCEAQDALLHKLLNDNFLSKEERDVLRWGKNIASAKTRTKKRAGAAVYNRASSLETLVGYLYLTDVTRLEEVMLKLGFSVDSSTPVLEETDKSCKLLPLST

>C.sativus

MVTLSATSTSIVASWDTQQRLSYNPNAPRNPNKKPKSPSFSPTSQATGNATLTATNIRTNQIVSDLLNRRNPSSTEGCKLEEPDGLYLGYDQWLPDPPKVQKPRSVFNAASLAYIGDCIYELYARRHFLFPPLSIEEYNDRVMAVVRCEAQDVLLQKLLNDNFLTEAERDILRWGKNVGSSSKTRTKKRAGAAVYNRASSLETLIGYLYLNNVKRLDEIMQKLGFSTDSSLQMISEESKVINQGS*

>P.persica

MASSSAMGVRVVTVRASLDTQQRLSYNRNTPRKIPKPENPSTATGTTKTPPTVTLTATTTSVFDLLKRPAQGETVRTDELYLGYERWVPSPSKVQKRLPSPPKVQKPRSVLNAASLAYIGDCIYELYVRRHFLFPPLSIEEYNDRVMAVVRCEAQDALLQKLLSDNFLSDEERNVLKWGKNISSAAKTRTKKRAGVAVYNRASSLETLVGYLYLTNMERLEEVMIKLGFSTDSSMQLISQEPEEIFPKNWKHISPRTFLQKSPSPSHLNLPMHLRENNNQSQHQGIYQNTETKRMENIGASTRARTVAKALNTIREMSDKALIDMVVVSQRRFQIQKEDKAKRG*

>M.guttatus

MSASLPILSLYSAVRASSVDIEQRAPYNPQRTYPKKPDALSLAPPSTLATTTDKHAVKISIPDLLKRDISKPARDEIGGSYLGYDTWLPTPPKVEKPRSVFNPASLAYIGDCIYELYARRHFLFPQLKIEEYNDRVMAVVRCEAQDAMLKILMSDNFLSPEERDVLRWGKNIGSSKTRTKKRAGSAVYNRASSLETLVGYLYLTNVQRLDEIMEKIGFSIDTNTPLEQAK*

>V.vinifera

MAGVGMASLSVRASWDTLPRLPYNPHNRRKPKTPISLKPPLNPSPVTLVASPTGTVVPVAELLRRDIQNSSQVKSDKSYMGYDIWLPSPPKVKKPRSAYNAASLAYLGDCIFELYARRHFLFPPLNMEEYNDRVMAVVRCEAQDALLQKLLDDYYLSEEERDVLRWGKNIGSAKTRTKKRAGVAVYNRASSLETLVGYLYLTDVNRLEEVMRKLGFSTDASTQLGLQETDTGS*

>P.patens

MALHHRWACSWPWLATPGGGGGDDAASPVRPRRQRPPRIDLRKLLDRVAVVHPDSSSAAQDNAKVWLPSPPPVEKPRAVHNAASLAYLGDSIYEVYVRRHFLTPPQKVDVYNKRVMDLVCCEAQDAMLQQLLKGDFLTDEERDVVRWGKNIETGQRRASRRAGSKAYSSASALETLIGYLYLTDHPRLEELMTKVGFNVDSNFSHIGFGFQTADKSSSS*

>C.sinensis

MPVTTCTSSLYLPLSLSSSRVRASFDTQQRLSYNPNAPRKIKKPPLNLNTNSNSNKSDVAIAKPPTSTVTPTSTDQSVFDLLKHPTQGVVANLDESYMGYERWLPNPPKVGKPRSVFNAASLAYIGDCIYELYARRHFLFPPLSIEEYNNRVMAVVRCETQDALLQKLLNDSYLSAEERDVVRWGKNVGSANTRTKRRAGAAVYNRASSLETLVSILVPCFSFISCQIAKCIMIYSGGCTNGKLIKGRKTLLFSVHQSPLFAYFYLVIASSSLLVLTY*

>A.thaliana RNC4

MAILSASSTIVRAALDTQPKLRYNPNAPRNVKKNSNLSSFVPPSSPSSSSPATNLSVSVSDLLKRPASKDIGDGFDDTCVGYEKWFPSPPKVEKPRSVFNAASLAYIGDSIYEIYARRHFLFPPLSIEEYNDRVRAVVRCEAQYALLQKLVDDDFLTKDERDILRWGKNVGSVKTRSTRRAGVAVYNKASSLETLIGYLYLSNGKRLEEMMQKLGFSSGSSTERMVKEAGSNKPSFK*

>A.thaliana RNC3

MVILSASSTVRAALDTQPRLPYNPNAPRKVKKIPNGNSFLPPPSPAPSPGISISVADLLKRPSSKEITVDVDDTYMGYETWSPSPPKLEKPRSVFNPASLAFIGDSIYELYARRHFLFPPLSIEDYNDRVRAVVRCEAQYALLNKLVDDDFLTKEERDILRWGKNLGSARTRTRRRAGNAVYNKASSLETLIGYLYLTNGKRLEKIMEKLGFSSDSSTEIVIEETNPKPSESSNLPNFILNEQVVSF*

T.halophila_2

MNRHRLIVGAVSKGSRYFESNSGKGQKRRVSFKRGKMVILSASSTVRAALDTQPRLPYNPNAPRKVKKIPKSNSFLPPPSPPPPSPRITISVDDLLKRPASKDLTDDFDDTYMGYETWSPTPPKLEKPRSVFNAASLAFIGDSIYELYARRHFLFPPLSIEDYNDRVRAVVRCEAQYALLKKLVDDDFLTKEERDVLRWGKNLGSVRTRSRRRAGVAVYNKASSLETLIGYLYLTNGKRLEKIMQKLGFSSDSSTEIIIEEAKPKPSESNLPSFILNEQVSQ*

T.halophila

MAILSASSTLVRAALDTQPRLRYNPNAPRNVKTNPNTSSFVPSSSPSSSSPARNLTAETGVSVSDLLKRPASKDIGDELDDTCIGFEKWFPSPPKVEKPRSVFNAASLAYIGDSIYEIYARRHFLFPPLSIEEYNDRVKAVVRCEAQYALLQKLADDDFLTKDERDILRWGKNVGSVKTRSTRRAGVAIYNKASSLETLIGYLYLANGKRLEEIMQKLGFSSGSATEKMIKEGKHKLSVLK*

>C.clementina

MPATTCTSCLYLPLSLSSSRVRASFDTQQRLSYNPNAPRKIKKPTLNLNTNSNSNKSDVAIAKPPTSTVTPTSTDQSVFDLLKHPTQGVVANLDESYMGYERWLPNPPKVGKPRSVFNAASLAYIGDCIYELYARRHFLFPPLSIEEYNNRVMAVVRCETQDALLQKLLNDSYLSAEERDVVRWGKNVGSANTRTKRRAGAAVYNRASSLETLVSILVPCFSFISCQIAKCIMIYSGGCTNGKLIKGRKTLLFSVHQSPLFAYFYLVIASSSLLVLTY*

>C.rubella_2

MAILSASSTIVRAALDTQPKLRYNPNAPRNVKKTPNLSSFVPPPSPSSSSPATNLTTEASVSASDLLKRPASKDIGDGFDDPCIGYEKWFPSPPKVEKPRSVFNAASLAYIGDSIYEIYARRHFLFPPLSIEEYNDRVRAVVRCEAQYALLQKLVDDDFLTKDERDVLRWGKNVGSVKTRSTRRAGVAVYNKASSLETLIGYLYLSNGKRLEEMMQKLGFSSGSSTERMIKEAKHKLSFK*

>C.rubella

MVILSASTTVRAALDTQPRLPYNPNAPRKVKKNPNSNSFLPPALPAPSPATSISVADLLKRPPSKELPVDVDDTYMGYETWSPSPPKLEKPRSVFNAASLAFVGDSIYELYARRHFLFPSLSIEDYNDRVRAVVRCEAQYALLKKLVDDDFLTKEERDVLRWGKNVGSSRTRTRRRAGNAVYNKASSLETLIGYLYLTNGKRLDKIMQKLGFSSDSSTEIIIEEAKPKPTESILPNFILNEQVVSQ*

>A.coerulea

SSWDTQPRITTSNPIPSSSSSSFSPATFPSFNTNTLKKRLMSLNKTPQTKTKKPSSSPSSSSSSTKKKPSINVSKLLELNYPTIPKQEQEDDLYLGSERWLPSPPKVNSPRSIYNAASLAYIGDCIYELYARRHFLFPALSIEEYNDRVTAVVRCEAQDALLKRLLHEDFLSEEERDLLRWGKNIDSGRTRTTKRAGVAVYNRASSLETLVGYLYLTNTKRLEEVMLKLGFTTAASNQLIMEEALNKPKLHVR*

>M.domestica

MQTLSVAFPPMSARVVTVRASVDTQQRLSYNRNAPRKIQKSESPATAKPPPTVTLTTTTTRTADSVVDLLKRTPQGETVQKDELYLGYERWSPTPTKVQKRLPSPPKVQKPRSVLNAASLAYIGDCIYELYARRHFLFPPLNIEEYNDRVMAVVRCEAQDALLRKLLSDDFLSEEERNVLRWGKNIGSAKTRTKKRAGSAAYNRASSLEALLGYLYLTNMERLEEVMIKLGFSTDSSVQIISEEPEEARILVNEDATESGVLFVPVTLEDNSGVTRGSSPYLNSKKDCVLGGFWVWVVEPMVPLWQLTKMDKCLEHEDLEPS

>B.rapa

MVILSASSTVRAALDTQPRLPYNPNAPRKVKKTPNGNSFLPPPLPPPPSPRISISVDDLLKRPESKELSVEVDDSYMGYETWSPSPPKLEKPRSVFNAASLAFIGDSIYELYARRHFLFPPLSIEDYNDRVRAVVRCEAQYALLKKLIDDDFLTKEERDVLRWGKNLGSARTRTRRRAGVAVYNKASSLETLIGYLYLTNGKRLEKIMEKLGFSSDSSTEIMIEMAKPKPSEPNLPSFILNEQVSTTVV*

>B.rapa_2

MAILSVSSSLVRAALDTKPKKLRYNPNAPRNVKRNPNSTTSFVPPSSPSSSPAATVLTTTSVSVSDLLKRPASKDSCVGYEKWFPSPPKVKKPRSVFNAASLAYIGDSIYEIYARRHFLFPPLSIEEYNDRVRAVVRCEAQYALLHKLLDEDFLTKDEREILRWGKNVGSVKTRSTRRAGVAVYNKASSLETLIGYLYLANGKRLEEIMQKLGFSSSSSTEIMVKEAKHKLSALK*

>M.trunculata

MQGLSSSLSWSITFSSSKFKPVVKASWDTQQRLPYNRNAPRKLTTQPPKPQSPSPTSSSPAPSTRNENYISDLLKRATPLPTIGKIEEVQEHDETYLGYDRWLPTPPKVVKPRSVFNAATLAYIGDCIYELYARRHFLFPPLSIEEFNDRVMAVVRCEAQDALLQKLLISNFLSDQERDVLRWGKNIISSKTKTKRRAGAAVYNRASSLETLVGYLYLTNVNRLEKLMLELGFSVDSSMPLNLEELIVSFILSSLAPQ*

>M.trunculata_2

MQGLSSSLSWSITFSSSKFKPVVKASWDTQQRLPYNRNAPRKLTTQPPKPQSPSPTSSSPAPSTRNENYISDLLKRATPLPTIGKIEEVQEHDETYLGYDRWLPTPPKVVKPRSVFNAATLAYIGDCIYELYARRHFLFPPLSIEEFNDRVMAVVRCEAQDALLQKLLISNFLSDQERDVLRWGKNIISSKTKTKRRAGAAVYNRASSLETLVGYLYLTNVNRLEKLMLELGFSVDSSMPLNLEELIVSFILSSLAPQLSIDFIVSFLLSRSNFDRNLL*

>V.carteri

MFVLSVTLGETTARRIPLPAWIHGAPPFRTAVIATSLPPPFLSGGATSNPRSIFNPLTLAYLGDAVWEAHTRRLETYIEAWIAQQSQAQQGTQQRHQQEQQRGSARASGRRGGGRAGERFVNAGDGDDRRDSSDVFNGVGGSRYGGVSLPSRQARKQWSTAIFQPPPCPPLSGVVLTAEELDVLRWGRNAAVSSIPRDVPVGVYKKATAVEVLVAHLYLTDPNRCAALIAAAVGLGASALAPLLYIAPAADSSPKPES*

>L.usitatissium

MTTVRASWDTQQRLTYNPNAPRNPNKKKLVTETLSTTSNSTTSSSSPPKLTLTPHSVSELLRRNVQSKAVVQDIDNSYLGCDVWLPKPPKVEKPRSAFNAATLAYIGDGIYELYARRHFLFPPMNIDEYNDRVMAVVRCEAQDALLQKLKEEDFLSTEERGILKWGKNVGSAKTRTRRRAGSAVYNRASSLETLIGHLYLTDSNRLDEVMQKLGFSAGSSSEMILHENTGCIPGEQADEMSIKGQ*

L.usitatissium_2

MTTVRASWDTQQRLTYNPNAPRNPNKKKLVTDTLSTTSNATTPSSSSPKFTLTPHSVSELLKRNVQSKAVVQDLDDSYLGCDVWLPRPPKLEKPRSAFNAATLAYIGDGIYELYARRHFLFPPMNIDEYNDRVMAVVRCEAQDALLQKLKEEDFLSAEERGILKWGKNVGSAKTRTRRRAGSAVYNRASSLETLIGYLYLTDANRLDEVMQKLGFSAGSSSEMILHETSGKPYPPSDETILIDKKSPCLKFWR*

>E.grandis

MAQHEHDPSSSSLSVCDGAPLSSEAETNREPCRRSRPARAPAMRVLAVPTLPSPVARAPSCPCSCSWDTQQRLPYNRNAPRNPNSGKLKSPAPTPPLAVAAPATRSGPSVADLLKRSDARPPRAGDEFYLGYELWLPSPPKVEKPRSVYNAALLAYIGDSIYELYARRHFLFPPLSIEEFNDRVTSVVRCEAQDELLQKLLADNYLSEGERDVLRWGKNVGTAKTRSRKRAGTAVYNRASSLETLIGYLYLTNVKRLEDVMLKLGFSTGASSEWLLNEETLVSSSSVI*

>O.sativa

MATTAATTMVAAVHHPRPCFRVRAAWDMNPSASPKPKAKPPPPPPPPLPTAPAPTHADLFARHSNSEGQVPKKTTSMGFERWWLPPPPEVKKPRSLYNAASLAYLGDCIYELYARRHFFFPPLSINDYNKRVMDVVKCESQDLLLNKLLGEDFLTQEERDILRWGRNIVSSKTRTRKRAGIAVYNRASSLETLIGYLYLTNFKRLEQLMFQLGFTSGASSHHIADELVSGFKKKNSTSVQSQQQAAQ*

>S.tuberosum

MAILLPLFSISPIVKASSVDTTQRLPYTPHRTTPKKTQTPDSLSAISTISLSPSTSRSNISVSDLLKREIPRSTEVKPDGTYMGYDVWLPRAHKVEKPRSVFNAASLAYIGDCIFEDAMLQKLMNDNILSEEERGVLRWGKNVGSGKTKTKKRAGVAVYNRASSLETLVGYLYLTNVQRLEEIMLKLGFHIGDSSLPIVERIDVMAGEYYKLIFNFSAFFSVIFLTYRENNKNMMHGDVATQVLYLSTIEMVEV*

>G.max

MESLLSVTSSSLKFLVRASWDTQQRLPYNPNAPRKLKNNIVATPTPTPPITRKREQYISDLLKRATPLPTIGHIEEVQDETYLGYEKWLPTPPKVVKPRSVFNAATLAYIGDCIYELYARRHFLFPPLSIEEYNDRVMAVVRCEAQDALLQKLLESNFLSDQERDVLRWGKNIVSSKTKTKKRAGAAVYNRASSLETLVGYLYLTNVNRLEKLMLELGFSVDSSVALNVEEIVKTGVNKETTETEIRKEHKWSN*

>G.raimondii

MAILTWTPPSLVAVTASWDTQQRPSYNPNAPRKLKPTPNLKPSPPITLPTRTDPSVFDILKRPTQEVTPVKVDLDDTYLGYERWLPTPPKVEKPRSVFNAATLAYIGDCIYELYARRHFLYPPLSIEEYNDRVTSVVRCEAQDALLQELLNDSFLSNTERDVLRWGKNINSAKTRTKKRAGAAVYNRASSLETLIGYLYLTNVNRLEKLMIRLGFSTGASAEMILKEINGAKPVQ*

>P.trichocarpa

MATAVGSMNVDVLSRRVRVRAASVDTQQRLSYNPNAPKNPFKNLNPKPNNFVPTSTAPAPSSTVTLTAQTVSGLLKRTTVIVQEQLNDSYLGYERWLPSPPKVEKPRSVFNAATLAYIGDSIFELYARRHFLFPPLSIEEHNDRVMAVVRCEAQDALLQKLLNDNYLSEEERLILRWGKNVGSTKTRTKRRVGVAVYNRASSLETLVGFLYLTNVSRLEEVMLKLGFSTDSSTQMIPEEENSKSTI*

>P.vulgaris

METLLSLSSSLKFAVRASWDTEQRLPYNRNAPRKLKKNIVSTPTPISTAPITPKRDQYISDLLKRATPLPTLAHVEEEQDETYMGYEKWLPTPPKVVKPRSVFNAATLAYIGDCIYELYARRHFLFPPLSIEEYNVRVMAVVRCEAQDALLQKLLQSNFLSDQERDVLRWGKNIVSSKTKTTKRAGTAVYSRASSLETLVGYLYLTNVNRLEKLMSELGFSVDSSVTLNIEEIIAGELNNR*

>F.vesca

MSVRVVRVGASVDTQQRLPYNRNAPRKIKDSSSTLSTTQNPPTVTLTASTTRTVDHVFELLKRPNQGYEQWLPSPPKIQKPSSPKIQKLSPPKVQKPQTVLNAAALAYLGDCIYESELLPGLKSPILVASSFLDESNLYARRHFLFPPLSIEEYNDRVMAVVRCEAQDALLQKLMKDNFLTEEERNVLRWGKNISSAKTRTKKRAGAAVYNRASSLETLDVVLNLTAIGMLSFDLVPLLDVGNLRSIKIYIEFAIGHLYLTDMKRLEEVMVKLGFLTDSSQLIPSEDVNGYALDYYRVLKVENAFVLEA*

>S.lycopersicum

MAILLPFFSISPIVKASVDTTQRLPYTPHRTSPKKPQTTDSLSAISTISLSPSTSRSNISVSDLLKREIPKSAEVKPDGTYMGYDVWLPSAPKVEKPRSVFNAASLAYVGDCIFELYARRHFLFPPLSIEEYNDRVMAVVRCEAQDAMLQKLMNDNILSEEERGVLRWGKNVGSGKTKTKKRAGVAVYNRASSLETLVGYLYLTNVQRLEQIMLKLGFQIGDSTLPIVERIDGTTR*

>T.cacao

MAILTWTPPPTAVPAAVTASWDTQQRPSYNPNAPRKLKPNPNLKPSPTVTLTTRTDPSVFDILKRPTQEVTPVKVDTDESYMGYERWLPTPPKVAKPRSVFNAATLAYIGDCIYEVVLYAQYLYARRHFLFPPLSIEEYNDRVTSVVRCEAQDALLQELLDDSFLSNDERNVLRWGKNISSSKTRTKKRAGAAVYNRASSLETLIGYLYLTNVNRLEKLMVKLGFSTGASTEMILKEVNGKLKLIHSAKKHCLLCLKLIWIMSEKPL*

Alignment used for phylogenetic analysis in Supplemental Figure 1 (FASTA format).

>A.lyrata

------MVILSASSTVRAALDTQP-RL------PYNPNAPRKVKKTP------NNNSFLPPP-SPPPSPGI------SISVADLLKRPASKELTVDV------DDTYMGYET-W------SPSPPKLEK------PRSVFNAASLAFIGDSIFE------LYARR------HFL------FPPL-SIEDYNDRVRAVVRCEAQYALLKKLVDDDFLTKEERDILRWGKNLG-SARTRTRRRAGNAVYNKASSLETL------IG----YLYL-TNGKRLEKIMQK-LGFSSDSSTEIVIEEAKPKPSE-SNLSNFILNEQVVS------

>gi|495455085|ref|WP_008179779.1|_hypothetical_protein_Moorea_producens

------MAQTDAQLDPLEED-SHGGGTDS------IVLWTEFRFRQ------F------MAKVDQIAK------IQQI-SPASLAYIGDAVYE------LYIRT------HYL------LPPR-RICDYHNQVVAHVRAESQAKILRSL--EPYLTASEKDVLRRGRNAA-TKT---PRRLNREIYQQASSLETL------LG----YLYL-TDTQRLTQLLAH-LKLDSP------

>gi|517210072|ref|WP_018398890.1|_hypothetical_protein_filamentous_cyanobacterium_ESFC-1

------MDRQDGQN------SLSSPLRLKAIATD------L------ETSLPGIEQ------LSPVALAYLGDAVYE------LYLRS------YYL------LPPQ-RISAYHQQVVAQARAERQADHLQAL--EVDLTAAERDILRRGRNAA-TRR---PQRLSAAVYRQATSLETL------IG----YLYL-TNPDRLNQLLAK-LELDSLET------

>gi|503086050|ref|WP_013320910.1|_ribonuclease_III_Cyanothece_sp._PCC_7822

------ME------LKA------I------CSLTAPLLK------IGQI-SPASLAYIGDAVYE------LYVRT------RYL------LPPR-RLCDYHNQVVAKVRAEQQAAYLQVL--EPHLTESEREIVRQGRNAA-NGR---PRRLSPELYQQASSLETL------IG----YLYL-QNPQRLNELLEI-LEF------

>gi|639207193|ref|WP_024545458.1|_Mini-ribonuclease_3_Synechococcus_sp._NKBG15041c

------M------FVPKISADR------LRQ-FSPASLAYVGDAVYE------LYCRC------YFL------LPPR-RSADYHDRVVAEVRAETQAQYLTVL--EPLLTDLERDIVRRGRNAA-TGK---PRRLSVTLYRQATGFEAL------IG----YLYL-TNQSRLEELFAH-LPRETPGV------

>gi|501592006|ref|WP_012595703.1|_MULTISPECIES:_ribonuclease_III_Cyanothece

------MNLPHSDLNQSLFLSND------SLPNPGVMLESV------GLDPSKISS------IGQV-SPAFLAYLGDAVYE------LYIRT------YYL------LPPR-RLVDYHNQVVSFVRAETQAALLEYL--KPYLTDAEREIVRRGRNAA-TGK---PRRLSPEIYQQATSLETL------IG----YLYL-HNQGRLTQLLEK-LNLSQLT------

>gi|497231352|ref|WP_009545614.1|_MULTISPECIES:_ribonuclease_III_Cyanothece

------MSQHRPGV------NFQV------IMEAKDYDN------ISQL-SPAFLAYIGDAVYE------LYVRT------SYL------FPPR-KVRDYHNQVVAQVRAETQAKALQRL--TPYLTTVEQDIVRRGRNAA-TGC---PRRLQPEIYQQATSLETL------IG----YLYL-QDTHRLRELLET-LIP------

>gi|495549199|ref|WP_008273778.1|_hypothetical_protein_Cyanothece_sp._CCY0110

------MSQHRPGV------NFQ------V------IMEAKDYET------ISQL-SPAFLAYIGDAVYE------LYVRT------SYL------FPPR-KVRDYHNQVVAQVRAETQAKALQRL--TPYLTTVEQDIVRRGRNAA-TGC---PRRLQPEIYQQATSLETL------IG----YLYL-QDTHRLHELLET-VIL------

>gi|506435475|ref|WP_015955192.1|_ribonuclease_III_Cyanothece_sp._PCC_7424

------MTSSKLNP-KPKNQSLL------SITPQEAVRLKV------I------CISTAPLSQ------MGQL-SPASLAYIGDAVYE------LYVRS------VYL------LPPR-RLSDYHSQVVAQVRAEQQAAHLQTL--EPYLTETEQEIVRQGRNAA-TGR---PRRLSPKLYQQATSLETL------IG----YLYL-QDPQRLTQLLEY-LEL------

>gi|502718511|ref|WP_012953495.1|_hypothetical_protein_Candidatus_Atelocyanobacterium_thalassa

------MI------KKCYPDVGFR------M------FVQTKDHSS------IDRL-SPAFLAYIGDAVYE------LYVRT------AYL------LPPR-KLADYHNQVVAQVRAETQAAALQQL--QPYLTETEKDIVRRGRNAT-NSH---PRRLKPEVYQQATSLETL------IG----YLYL-QDSQRLNEILRN-LV------

>gi|504957396|ref|WP_015144498.1|_hypothetical_protein_Pleurocapsa_minor

------MDVQQ------SKWNPANLVFVPGADVGC------EIACTDVDRA------Y------L------IGQL-SPASLAYIGDAVYE------LYVRT------RYL------LPPK-RLSDYHDRVVAQVRAESQAACLRVL--ESYLTDEEREILRRGRNAA-TGK---PRRLSPELHQQATSLETL------IG----YLYL-QNPQRLNELLEH-LNLE------

>gi|493032906|ref|WP_006102210.1|_RNase3_domain_protein_Coleofasciculus_chthonoplastes

------MEQQEETANSVDNNS------ALSGTEARFRQ------L------NTAADPQCQ------IQQI-SPAALAYLGDAVYE------LYIRR------HYL------LPPK-RLCDYHDQVVAQVRAESQANTLRSL--EPYLTEPELALLRRGRNAA-TGT---SRRLNRKIYQQATSLETL------LG----YLYL-TDPQRLTQLLAY-LELDTP------

>gi|493557334|ref|WP_006510819.1|_hypothetical_protein_Xenococcus_sp._PCC_7305

------MNLPNPN-QDQIESID------TPLGVDVTAL---A------S------SITVVGDSQ------LKRL-SPVALAYIGDAVYE------LIVRA------KLL------LPPK-RIADYHSQVVAQVRAEAQATHLQDL--EPYLTPAEKEILRRGRNAV-TNK---PRRLSMKIYQQASSFETL------IG----YLYL-EDSQRLQDLFTK-LNL------

>gi|505006810|ref|WP_015193912.1|_ribonuclease_III_Stanieria_cyanosphaera

------MSLPKPDRDQPELTTSA------SVQAEASLL------F------NQMTTSLVS------QVESLSPIALAYIGDAVYE------LYVRT------CYL------IPPK-RLADYHNQVVAQVRAESQADYLQLL--QPYLTFSEQEIVRRGRNAV-DKK---PRRISAQIYQQATSFETL------LG----YLYL-TNPQRLDELLAK-LKINSSKLLT------

>gi|501263000|ref|WP_012306018.1|_ribonuclease_III_protein_Synechococcus_sp._PCC_7002

------M------FVPDITADQ------LRQL-SPASLAYVGDAVYE------LYSRC------YFL------LPPC-RIADYHGKVVAEVRAETQAQYLTLL--EPHLSELERDIVRRGRNAA-TGK---PRRLSLAIYRQATGFEAL------VG----YLYL-TNPQRLAELFTC-LPQERSGV------

>gi|505002871|ref|WP_015189973.1|_ribonuclease_III_Gloeocapsa_sp._PCC_7428

------MNPKEEELLDRS------FIIEDELTLKLEQ------L------LPTLAEDLL------QLQQASPSALAYLGDAVYE------LYIRR------YYL------LPPK-RSQVYHRLVVAQVRAEAQASHLRSL--TPYLTSTELDIVRRGRNAT-TKP---PKRIEPEIYQQASSLETL------VG----YLFL-TDCQRLAQLLHK-LDFEQNQNE------

>gi|488881745|ref|WP_002793970.1|_Similar_to_tr|P74327|P74327_Microcystis_aeruginosa

------MESPLEQI------RLQV------N------GEGSPILEG------LDRL-SPASLAYLGDAVYE------LYIRT------HYL------LPPQ-RLGDYHAQVVGKVRAEQQALDLAQL--QPYLTDQEKEILRRGRNAV-TGK---RRRLTAQVYQQASSLETL------FG----YLYL-QDPSRLHQLLEK-LEL------

>gi|494522639|ref|WP_007312092.1|_cysteinyl-tRNA_synthetase-like_small_protein_Crocosphaera_watsonii

------MSPAFLAYIGDAVYE------LYVRT------CYL------LPPR-KVGVYHNQVVAQVRAETQAKALQKL--TPLLTTVEQDIVRRGRNAA-TGC---PRRLKPEIYQQATSLETL------IG----YLYL-RDTERLSELLNI-LIT------

>gi|504986721|ref|WP_015173823.1|_ribonuclease_III_Geitlerinema_sp._PCC_7407

------MNTLDTPSQPL-PERPELEI------ALPWAALGRP---C------D------QSQALTSAE------IRRL-SPVALAYLGDAVYE------LFVRT------RYL------MPPR-RIQTYHHEVVDQVRAERQAEYLQSL--EPYFTPEELDILKRGRNAVPSGR---TKRVSPEIYQQASSLETL------MG----YLYL-TDPARLFQLLSY-LPFEAKSSLTAHS------

>gi|550282684|ref|WP_022608994.1|_Ribonuclease_III_Rubidibacter_lacunae

------MSAAARDDA-TPDVVSSR------ALPTDGDAREFLSVQLDCLRAG------F------DTDPTSALA------AQRL-SPAVLAYLGDAVYE------VCARS------RYL------LPPK-RLSDYHRLVVSAVRAETQAERLQTL--EPYLNADERDILRRGRNAA-SGR---PRRLSPLIYQQATSLETL------IG----YLFL-CDRERLLQVLQW-LDLCA------

>gi|515490916|ref|WP_016924170.1|_hypothetical_protein_Prochlorothrix_hollandica

------MEADPKPSP-LSQSITAI------AMDGGQ------P------LLSPQEAQH------LSPVLLAYVGDAIYE------LYVRL------HYL------LPPQ-RIQAYHRQVVGQVRAEQQSLHLQTL--RQHLTEAESAIVRRGRNAA-TSA---PKRVKVSVYQQATALEAL------VG----YLYL-TDPARLKVLWQY-LVFCDSP------

>B.subtilis

------MLEFDTIKD------SKQL-NGLALAYIGDAIFE------VYVRH------HLL------KQGFTKPNDLHKKSSRIVSAKSQAEILFFLQNQSFFTEEEEAVLKRGRNAK-SGTT--PKNTDVQTYRYSTAFEAL------LG----YLFLEKKEERLSQLVAEAIQFGTSGRKTNESAT------

>C.reinhardtii

------MSEMRTYMRRI------GGSKAALPRRCSDRRSSLHCT------C------MLPPPQLTG------SAAANPRSIYSPLALAYLGDAVWE------VSMAA------QAVGERD------FSASMPITVSPAGAAAIAAAAATDASGAAASQLLVLTPAEADVLRWGRNAA-VGSI--PKSVSAGTYKKATAVEVL------VA----HLYL-TAPQRCAQLIQAAVSAVPLQP------

>A.thaliana_RNC3

------MVILSASSTVRAALDTQP-RL------PYNPNAPRKVKKIP------NGNSFLPPP-SPAPSPGI------SISVADLLKRPSSKEITVDV------DDTYMGYET-W------SPSPPKLEK------PRSVFNPASLAFIGDSIYE------LYARR------HFL------FPPL-SIEDYNDRVRAVVRCEAQYALLNKLVDDDFLTKEERDILRWGKNLG-SARTRTRRRAGNAVYNKASSLETL------IG----YLYL-TNGKRLEKIMEK-LGFSSDSSTEIVIEETNPKPSESSNLPNFILNEQVVSF------

>C.rubella

------MVILSASTTVRAALDTQP-RL------PYNPNAPRKVKKNP------NSNSFLPPA-LPAPSPAT------SISVADLLKRPPSKELPVDV------DDTYMGYET-W------SPSPPKLEK------PRSVFNAASLAFVGDSIYE------LYARR------HFL------FPSL-SIEDYNDRVRAVVRCEAQYALLKKLVDDDFLTKEERDVLRWGKNVG-SSRTRTRRRAGNAVYNKASSLETL------IG----YLYL-TNGKRLDKIMQK-LGFSSDSSTEIIIEEAKPKPTE-SILPNFILNEQVVSQ------

>B.rapa

------MVILSASSTVRAALDTQP-RL------PYNPNAPRKVKKTP------NGNSFLPPPLPPPPSPRI------SISVDDLLKRPESKELSVEV------DDSYMGYET-W------SPSPPKLEK------PRSVFNAASLAFIGDSIYE------LYARR------HFL------FPPL-SIEDYNDRVRAVVRCEAQYALLKKLIDDDFLTKEERDVLRWGKNLG-SARTRTRRRAGVAVYNKASSLETL------IG----YLYL-TNGKRLEKIMEK-LGFSSDSSTEIMIEMAKPKPSE-PNLPSFILNEQVSTTVV------

>L.usitatissium

------MTTVRASWDTQQ-RL------TYNPNAPRNPNKKK---LVTETLSTTSNSTTSSS-SPPKLTLT------PHSVSELLRRNVQSKAVVQDI-----DNSYLGCDV-W------LPKPPKVEK------PRSAFNAATLAYIGDGIYE------LYARR------HFL------FPPM-NIDEYNDRVMAVVRCEAQDALLQKLKEEDFLSTEERGILKWGKNVG-SAKTRTRRRAGSAVYNRASSLETL------IG----HLYL-TDSNRLDEVMQK-LGFSAGSSSEMILHENTGCIPGEQADEMSIKGQ------

>L.usitatissium_2

------MTTVRASWDTQQ-RL------TYNPNAPRNPNKKK---LVTDTLSTTSNATTPSS-SSPKFTLT------PHSVSELLKRNVQSKAVVQDL-----DDSYLGCDV-W------LPRPPKLEK------PRSAFNAATLAYIGDGIYE------LYARR------HFL------FPPM-NIDEYNDRVMAVVRCEAQDALLQKLKEEDFLSAEERGILKWGKNVG-SAKTRTRRRAGSAVYNRASSLETL------IG----YLYL-TDANRLDEVMQK-LGFSAGSSSEMILHETSGKPYPPSDETILIDKKSPCLKFWR------

>A.thaliana_RNC4

------MAILSASSTIVRAALDTQP-KL------RYNPNAPRNVKKNS------NLSSFVPPSSPSSSSPATNL----SVSVSDLLKRPASKDIGDGF------DDTCVGYEK-W------FPSPPKVEK------PRSVFNAASLAYIGDSIYE------IYARR------HFL------FPPL-SIEEYNDRVRAVVRCEAQYALLQKLVDDDFLTKDERDILRWGKNVG-SVKTRSTRRAGVAVYNKASSLETL------IG----YLYL-SNGKRLEEMMQK-LGFSSGSSTERMVKEAGSNKPSFK------

>O.sativa

------MATTAATTMVAAVHHPRPCFRVRAAWDMNP------SASPKPKAKPPPPP-PPPLPTAP------APTHADLFARHSNSEGQVPK------KTTSMGFERWW------LPPPPEVKK------PRSLYNAASLAYLGDCIYE------LYARR------HFF------FPPL-SINDYNKRVMDVVKCESQDLLLNKLLGEDFLTQEERDILRWGRNIV-SSKTRTRKRAGIAVYNRASSLETL------IG----YLYL-TNFKRLEQLMFQ-LGFTSGASSHHIADELVSGFKKKNSTSVQSQQQAAQ------

>M.trunculata

------MQGLSSSLSWSITFSSSKFKPVVKASWDTQQ-RL------PYNRNAPRKLTTQP------PKPQSPSPTSSSPAPSTRN------ENYISDLLKRATPLPTIGKIEEVQEHDETYLGYDR-W------LPTPPKVVK------PRSVFNAATLAYIGDCIYE------LYARR------HFL------FPPL-SIEEFNDRVMAVVRCEAQDALLQKLLISNFLSDQERDVLRWGKNII-SSKTKTKRRAGAAVYNRASSLETL------VG----YLYL-TNVNRLEKLMLE-LGFSVDSSMPLNLEELIVSFILSSLAPQ------

>M.trunculata_2

------MQGLSSSLSWSITFSSSKFKPVVKASWDTQQ-RL------PYNRNAPRKLTTQP------PKPQSPSPTSSSPAPSTRN------ENYISDLLKRATPLPTIGKIEEVQEHDETYLGYDR-W------LPTPPKVVK------PRSVFNAATLAYIGDCIYE------LYARR------HFL------FPPL-SIEEFNDRVMAVVRCEAQDALLQKLLISNFLSDQERDVLRWGKNII-SSKTKTKRRAGAAVYNRASSLETL------VG----YLYL-TNVNRLEKLMLE-LGFSVDSSMPLNLEELIVSFILSSLAPQLSIDFIVSFLLSRSNFDRNLL------

>T.halophila

------MAILSASSTLVRAALDTQP-RL------RYNPNAPRNVKTNP------NTSSFVPSSSPSSSSPARNLTAETGVSVSDLLKRPASKDIGDEL------DDTCIGFEK-W------FPSPPKVEK------PRSVFNAASLAYIGDSIYE------IYARR------HFL------FPPL-SIEEYNDRVKAVVRCEAQYALLQKLADDDFLTKDERDILRWGKNVG-SVKTRSTRRAGVAIYNKASSLETL------IG----YLYL-ANGKRLEEIMQK-LGFSSGSATEKMIKEGKHKLSVLK------

>G.raimondii

------MAILTWTPPSLVAVTASWDTQQ-RP------SYNPNAPRKLK------PTPNLKPSP-PITLPTRT------DPSVFDILKRPTQEVTPVKVDL----DDTYLGYER-W------LPTPPKVEK------PRSVFNAATLAYIGDCIYE------LYARR------HFL------YPPL-SIEEYNDRVTSVVRCEAQDALLQELLNDSFLSNTERDVLRWGKNIN-SAKTRTKKRAGAAVYNRASSLETL------IG----YLYL-TNVNRLEKLMIR-LGFSTGASAEMILKEINGAKPVQ------

>C.rubella_2

------MAILSASSTIVRAALDTQP-KL------RYNPNAPRNVKKTP------NLSSFVPPPSPSSSSPATNLTTEASVSASDLLKRPASKDIGDGF------DDPCIGYEK-W------FPSPPKVEK------PRSVFNAASLAYIGDSIYE------IYARR------HFL------FPPL-SIEEYNDRVRAVVRCEAQYALLQKLVDDDFLTKDERDVLRWGKNVG-SVKTRSTRRAGVAVYNKASSLETL------IG----YLYL-SNGKRLEEMMQK-LGFSSGSSTERMIKEAKHKLSFK------

>B.rapa_2

------MAILSVSSSLVRAALDTKPKKL------RYNPNAPRNVKRNP------NSTTSFVPPSSPSSSPAATVLTTTSVSVSDLLKRPAS------KDSCVGYEK-W------FPSPPKVKK------PRSVFNAASLAYIGDSIYE------IYARR------HFL------FPPL-SIEEYNDRVRAVVRCEAQYALLHKLLDEDFLTKDEREILRWGKNVG-SVKTRSTRRAGVAVYNKASSLETL------IG----YLYL-ANGKRLEEIMQK-LGFSSSSSTEIMVKEAKHKLSALK------

>T.cacao

------MAILTWTPPPTAVPAAVTASWDTQQ-RP------SYNPNAPRKLKPNP------NLKPSP-TVTLTTRT------DPSVFDILKRPTQEVTPVKVDT----DESYMGYER-W------LPTPPKVAK------PRSVFNAATLAYIGDCIYE------VVLYAQYLYARR------HFL------FPPL-SIEEYNDRVTSVVRCEAQDALLQELLDDSFLSNDERNVLRWGKNIS-SSKTRTKKRAGAAVYNRASSLETL------IG----YLYL-TNVNRLEKLMVK-LGFSTGASTEMILKEVNGKLKLIHSAKKHCLLCLKLIWIMSEKPL------

>V.vinifera

------MAGVGMASLSVRASWDTLP-RL------PYNPHNRRKPKTPI------SLKPPLNPSPVT-LVASPTGT------VVPVAELLRRDIQNSSQVKS------DKSYMGYDI-W------LPSPPKVKK------PRSAYNAASLAYLGDCIFE------LYARR------HFL------FPPL-NMEEYNDRVMAVVRCEAQDALLQKLLDDYYLSEEERDVLRWGKNIG-SAKTRTKKRAGVAVYNRASSLETL------VG----YLYL-TDVNRLEEVMRK-LGFSTDASTQLGLQETDTGS------

>V.carteri

------MFVLSV-TLGETTAR------RIPLPAWIHGAPPFRTAVIA------T------SLPPPFLSG------GATSNPRSIFNPLTLAYLGDAVWE------AHTRRLETYIEAWIAQQSQAQQGTQQRHQQEQQRGSARASGRRGGGRAGERFVNAGDGDDRRDSSDVFNGVGGSRYGGVSLPSRQARKQWSTAIFQPPPCPPLSGVV------LTAEELDVLRWGRNAAVSSI---PRDVPVGVYKKATAVEVL------VA----HLYL-TDPNRCAALIAAAVGLGASALAPLLYIAPAADSSPKPES------

>C.papaya

------MPVLCTNISPGLRVRTSLDTQR-RL------PYNPHTPPKPKRSP------NDFAPSPAVTLTNNCT------DRSGADLLKRRLDQGVANKR------DESYMGYEI-W------LPRPPKVDK------PRSVYNAASLAYIGDCIYE------LYARR------HFF------FPPL-NIEEYNDRVTAVVRCEAQDALLQKLLNDNYLTEEERNVLRWGKNIG-SAKTRSRKRAGVAVYNRASSLETL------IG----FLYL-TNVSRLEEVMLK-LGFSTGSSTQDILEEGNIVEFNIQASFLLITGWKTAMTATT------

>A.lyrata_2

------MAILSASSTIVRAALDTQP-KL------RYNPNAPRNVK------NLSSFVPPLSPSSSSPATNLTTGTNVSVSDLLKRPASKDVGNGF------DDNCIGYDK-W------FPSPPKVEK------PRSVFNAASLAYIGDSIYEQGYQVMIILFFRMQGCQMRIIFSDYSIKTATMSLCYFLYLSLAVLGYKNIYARR------HFL------FPPL-SIEEYNDRVRAVVRCEAQYALLQKLVDDDFLTKDERDVLRWGKNVG-SAKTRSTRRAGVAVYNKASSLETL------IG----YLYL-SNGKRLEEMMQK-LGFSSGSSTERMIKEAGKNKPSFK------

>C.sinensis

------MPVTTCTSSLYLPLSLSSSRVRASFDTQQ-RL------SYNPNAPRKIKKPPLNLNTNSNSNKSDVAIAKPPTSTVTPTST------DQSVFDLLKHPTQGVVANL------DESYMGYER-W------LPNPPKVGK------PRSVFNAASLAYIGDCIYE------LYARR------HFL------FPPL-SIEEYNNRVMAVVRCETQDALLQKLLNDSYLSAEERDVVRWGKNVG-SANTRTKRRAGAAVYNRASSLETL------VSILVPCFSF-ISCQIAKCIMIY-SGGCTNGKLIKGRKTLLFSVHQSPLFAYFYLVIASSSLLVLTY------

>M.guttatus

------MSASLPILSLYSAVRASSVDIEQRA------PYNPQRTYPKKPDA------LSLAPPSTLATTTDKHAV------KISIPDLLKRDISKPARDEI------GGSYLGYDT-W------LPTPPKVEK------PRSVFNPASLAYIGDCIYE------LYARR------HFL------FPQL-KIEEYNDRVMAVVRCEAQDAMLKILMSDNFLSPEERDVLRWGKNIG-SSKTRTKKRAGSAVYNRASSLETL------VG----YLYL-TNVQRLDEIMEK-IGFSIDTNTPLEQAK------

>M.domestica

------MQTLSVAFPPMSARVVTVRASVDTQQ-RL------SYNRNAPRKIQKSE------SPATAKPPPTVTLTTTTTRT------ADSVVDLLKRTPQGETVQK------DELYLGYER-WSPTPTKVQKRLPSPPKVQK------PRSVLNAASLAYIGDCIYE------LYARR------HFL------FPPL-NIEEYNDRVMAVVRCEAQDALLRKLLSDDFLSEEERNVLRWGKNIG-SAKTRTKKRAGSAAYNRASSLEAL------LG----YLYL-TNMERLEEVMIK-LGFSTDSSVQIISEEPEEARILVNEDATESGVLFVPVTLEDNSGVTRGSSPYLNSKKDCVLGGFWVWVVEPMVPLWQLTKMDKCLEHEDLEPS------

>C.sativus

------MVTLSATSTSIVASWDTQQ-RL------SYNPNAPRNPNKKP-----KSPSFSPTSQATGNATLTATNIRT------NQIVSDLLNRRNPSSTEGCKLEEP--DGLYLGYDQ-W------LPDPPKVQK------PRSVFNAASLAYIGDCIYE------LYARR------HFL------FPPL-SIEEYNDRVMAVVRCEAQDVLLQKLLNDNFLTEAERDILRWGKNVGSSSKTRTKKRAGAAVYNRASSLETL------IG----YLYL-NNVKRLDEIMQK-LGFSTDSSLQMISEESKVINQGS------

>C.clementina

------MPATTCTSCLYLPLSLSSSRVRASFDTQQ-RL------SYNPNAPRKIKKPTLNLNTNSNSNKSDVAIAKPPTSTVTPTST------DQSVFDLLKHPTQGVVANL------DESYMGYER-W------LPNPPKVGK------PRSVFNAASLAYIGDCIYE------LYARR------HFL------FPPL-SIEEYNNRVMAVVRCETQDALLQKLLNDSYLSAEERDVVRWGKNVG-SANTRTKRRAGAAVYNRASSLETL------VSILVPCFSF-ISCQIAKCIMIY-SGGCTNGKLIKGRKTLLFSVHQSPLFAYFYLVIASSSLLVLTY------

>E.grandis

MAQHEHDPSSSSLSVCDGAPLSSEAETNREPCRRSRPARAPAMRVLAVPTLPSPVARAPSCPCSCSWDTQQ-RL------PYNRNAPRNPNSGK------LKSPAPTPPLAVAAPATRS------GPSVADLLKRSDARPPRAG------DEFYLGYEL-W------LPSPPKVEK------PRSVYNAALLAYIGDSIYE------LYARR------HFL------FPPL-SIEEFNDRVTSVVRCEAQDELLQKLLADNYLSEGERDVLRWGKNVG-TAKTRSRKRAGTAVYNRASSLETL------IG----YLYL-TNVKRLEDVMLK-LGFSTGASSEWLLNEETLVSSSSVI------

>P.persica

------MASSSAMGVRVVTVRASLDTQQ-RL------SYNRNTPRKIPKPE------NPSTATGTTKTP-PTVTLTAT------TTSVFDLLKRPAQGETVRT------DELYLGYER-W------VPSPSKVQK------RLPSPPKVQKPRSVLNAASLAYIGDCIYE------LYVRR------HFL------FPPL-SIEEYNDRVMAVVRCEAQDALLQKLLSDNFLSDEERNVLKWGKNISSAAKTRTKKRAGVAVYNRASSLETL------VG----YLYL-TNMERLEEVMIK-LGFSTDSSMQLISQEPEEIFPKNWKHISPRTFLQKSPSPSHLNLPMHLRENNNQSQHQGIYQNTETKRMENIGASTRARTVAKALNTIREMSDKALIDMVVVSQRRFQIQKEDKAKRG

>P.trichocarpa

------MATAVGSMNVDVLSRRVRVRAASVDTQQRL------SYNPNAPKNPFKNL------NPKPNNFVPTSTAPAPSSTVTLT---AQTVSGLLKRTTVIVQEQL------NDSYLGYER-W------LPSPPKVEK------PRSVFNAATLAYIGDSIFE------LYARR------HFL------FPPL-SIEEHNDRVMAVVRCEAQDALLQKLLNDNYLSEEERLILRWGKNVG-STKTRTKRRVGVAVYNRASSLETL------VG----FLYL-TNVSRLEEVMLK-LGFSTDSSTQMIPEEENSKSTI------

>T.halophila_2

------MNRHRLIVGAVSKGSRYFESNSGKGQKRRVSFKRGKMVILSASSTVRAALDTQP-RL------PYNPNAPRKVKKIP------KSNSFLPPPSPPPPSPRI------TISVDDLLKRPASKDLTDDF------DDTYMGYET-W------SPTPPKLEK------PRSVFNAASLAFIGDSIYE------LYARR------HFL------FPPL-SIEDYNDRVRAVVRCEAQYALLKKLVDDDFLTKEERDVLRWGKNLG-SVRTRSRRRAGVAVYNKASSLETL------IG----YLYL-TNGKRLEKIMQK-LGFSSDSSTEIIIEEAKPKPSE-SNLPSFILNEQVSQ------

>F.vesca

------MSVRVVRVGASVDTQQ-RL------PYNRNAPRKIKDSS------STLSTTQNPPTVTLTASTTRT------VDHVFELLKRPNQ------GYEQ-W------LPSPPKIQKPSSPKIQKLSPPKVQKPQTVLNAAALAYLGDCIYESELLP------GLKSPILVASSFLDESNLYARR------HFL------FPPL-SIEEYNDRVMAVVRCEAQDALLQKLMKDNFLTEEERNVLRWGKNIS-SAKTRTKKRAGAAVYNRASSLETLDVVLNLTAIGMLSFDLVPLLDVGNLRSIKIYIEFAIG----HLYL-TDMKRLEEVMVK-LGFLTDSSQLIPSEDVNGYALDYYRVLKVENAFVLEA------

>P.patens

------MALHHRWACSWPWLATP------GGGGGDDAASPVRPRRQRPP------RIDLRKLLDRVAVVHPDS------SSAAQDNAKVW------LPSPPPVEK------PRAVHNAASLAYLGDSIYE------VYVRR------HFL------TPPQ-KVDVYNKRVMDLVCCEAQDAMLQQLLKGDFLTDEERDVVRWGKNIE-TGQRRASRRAGSKAYSSASALETL------IG----YLYL-TDHPRLEELMTK-VGFNVDSNFSHIGFGFQTADKSSSS------

>S.tuberosum

------MAILLPLFSISPIVKASSVDTTQRL------PYTPHRTTPKKTQT------PDSLSAISTISLSPSTSRS------NISVSDLLKREIPRSTEVKP------DGTYMGYDV-W------LPRAHKVEK------PRSVFNAASLAYIGDCIFE------DAMLQKLMNDNILSEEERGVLRWGKNVG-SGKTKTKKRAGVAVYNRASSLETL------VG----YLYL-TNVQRLEEIMLK-LGFHIGDSSLPIVERIDVMAGEYYKLIFNFSAFFSVIFLTYRENNKNMMHGDVATQVLYLSTIEMVEV------

>S.lycopersicum

------MAILLPFFSISPIVKASVDTTQ-RL------PYTPHRTSPKKPQT------TDSLSAISTISLSPSTSRS------NISVSDLLKREIPKSAEVKP------DGTYMGYDV-W------LPSAPKVEK------PRSVFNAASLAYVGDCIFE------LYARR------HFL------FPPL-SIEEYNDRVMAVVRCEAQDAMLQKLMNDNILSEEERGVLRWGKNVG-SGKTKTKKRAGVAVYNRASSLETL------VG----YLYL-TNVQRLEQIMLK-LGFQIGDSTLPIVERIDGTTR------

>G.max

------MESLLSVTSSSLKFLVRASWDTQQ-RL------PYNPNAPRKLK------NNIVATPTP-TPPITRKR------EQYISDLLKRATPLPTIGHIEEVQ--DETYLGYEK-W------LPTPPKVVK------PRSVFNAATLAYIGDCIYE------LYARR------HFL------FPPL-SIEEYNDRVMAVVRCEAQDALLQKLLESNFLSDQERDVLRWGKNIV-SSKTKTKKRAGAAVYNRASSLETL------VG----YLYL-TNVNRLEKLMLE-LGFSVDSSVALNVEEIVKTGVNKETTETEIRKEHKWSN------

>P.vulgaris

------METLLSLSSSLKFAVRASWDTEQ-RL------PYNRNAPRKLKKNI------VSTPTPIS-TAPITPKR------DQYISDLLKRATPLPTLAHVEEEQ--DETYMGYEK-W------LPTPPKVVK------PRSVFNAATLAYIGDCIYE------LYARR------HFL------FPPL-SIEEYNVRVMAVVRCEAQDALLQKLLQSNFLSDQERDVLRWGKNIV-SSKTKTTKRAGTAVYSRASSLETL------VG----YLYL-TNVNRLEKLMSE-LGFSVDSSVTLNIEEIIAGELNNR------

>A.coerulea

------SSWDTQP-RITTSNPIPSSSSSSFSPATFPSFNTNTLKKRLMSL--NKTPQTKTKKPSSSPSSS-SSSTKKKP------SINVSKLLELNYPTIPKQEQE-----DDLYLGSER-W------LPSPPKVNS------PRSIYNAASLAYIGDCIYE------LYARR------HFL------FPAL-SIEEYNDRVTAVVRCEAQDALLKRLLHEDFLSEEERDLLRWGKNID-SGRTRTTKRAGVAVYNRASSLETL------VG----YLYL-TNTKRLEEVMLK-LGFTTAASNQLIMEEALNKPKLHVR------

>R.communis

------MPTILSTMSSSLRVSVRASWDTQQ-RL------TYNPNAPRKHTKNP------TSQPNTLSLPPPTTTSPLSVTVT------TQSVSNLLKRNTTPQGVMKKL-----DDTYLGYER-W------MPTPPKVEK------PRSVYNAATLAYIGDSIYE------LYARR------HFL------FPPL-SIEEYNDRVMSVVRCEAQDALLHKLLNDNFLSKEERDVLRWGKNIA-SAKTRTKKRAGAAVYNRASSLETL------VG----YLYL-TDVTRLEEVMLK-LGFSVDSSTPVLEETDKSCKLLPLST------