Supplemental Table 1 Gene regulation compared with Arabidopsis under Cd stress (Herbette et al. 2006)or rice under abiotic (cold, drought, salt, or ABA) stress (Rabbaniet al. 2003).
Gene Location / Description based on RAP2 / Fold Change (Root)1 / Fold Change (Shoot)1 / Arabidopsis ortholog / Regulation in Cd-treated Arabidopsis2(Herbette et al. 2006) / Regulation in rice under abiotic stress2
(Rabbani et al. 2003) / Function
10 μM Cd / 1 μM Cd / 10 μM Cd / 1 μM Cd
3 h / 24 h / 48 h / 72 h / 8 d / 3 h / 24 h / 48 h / 72 h / 8 d / Root / Shoot / cold / drought / salt / ABA
Os03g0323200 / Similar to Protoporphyrin IX Mg-chelatase subunit precursor. / 1.7 / 1.3 / 1.1 / 0.8 / 0.6 / 0.2 / 0.9 / 1.0 / 1.0 / 0.7 / At5g13630 / ▼ / ▼ / Photosystem
Os09g0481200 / Similar to Photosystem I reaction center subunit V. / 0.8 / 0.9 / 1.1 / 1.0 / 1.2 / 0.5 / 0.9 / 1.0 / 1.0 / 0.7 / At1g55670 / ▼
Os12g0189400 / Similar to Photosystem I reaction centre subunit N. / 1.1 / 1.1 / 1.1 / 0.9 / 1.1 / 0.3 / 0.9 / 0.9 / 0.9 / 0.7 / At5g64040 / ▼ / ▼
Os01g0720500 / Similar to Type I chlorophyll a/b-binding protein b (Fragment). / 1.0 / 0.4 / 1.3 / 1.0 / 1.4 / 0.3 / 1.0 / 0.6 / 0.8 / 0.5 / At2g34430 / ▼ / ▼
Os09g0346500 / Similar to Chlorophyll a-b binding protein. / 1.0 / 1.0 / 1.3 / 0.9 / 0.6 / 0.2 / 1.0 / 1.1 / 1.3 / 0.9
Os01g0600900 / Chlorophyll a-b binding protein 2. / 0.7 / 1.0 / 2.1 / 0.4 / 0.5 / 0.4 / 0.9 / 0.9 / 1.1 / 0.7
Os07g0544800 / Similar to Oxygen-evolving enhancer protein 3-2, chloroplast precursor. / 1.2 / 1.3 / 0.6 / 0.8 / 1.7 / 0.5 / 0.8 / 0.7 / 0.7 / 0.7 / At4g21280 / ▼
Os03g0102700 / Beta-expansin precursor. / 0.3 / 1.5 / 2.1 / 1.4 / 1.1 / 0.5 / 1.3 / 1.1 / 1.0 / 0.5 / At1g65680 / ▼ / Cell expansion
Os02g0744200 / Alpha-expansin OsEXPA5. / 1.0 / 1.2 / 1.1 / 1.2 / 1.0 / 0.5 / 1.6 / 1.3 / 0.9 / 0.3 / At2g03090 / ▼ / ▼
Os08g0557600 / Similar to Monodehydroascorbate reductase. / 3.9 / 1.5 / 2.1 / 0.8 / 0.5 / 1.6 / 1.2 / 1.0 / 1.1 / 1.4 / At5g03630 / ▲ / Oxidative stress
Os07g0186000 / Similar to Thioredoxin h isoform 1. / 2.1 / 1.3 / 1.8 / 0.8 / 1.0 / 0.9 / 1.1 / 1.1 / 0.9 / 1.1 / At1g19730 / No data / ▲ / ▲ / ▲
Os01g0667900 / Glutaredoxin-like, plant II family protein. / 2.1 / 0.9 / 1.1 / 0.8 / 0.9 / 1.2 / 0.9 / 1.0 / 1.0 / 1.2 / At1g28480 / ▲ / ▲
Os03g0132000 / Similar to 4-coumarate-CoA ligase-like protein. / 4.6 / 1.4 / 1.3 / 1.0 / 0.7 / 1.1 / 1.1 / 1.0 / 1.2 / 1.0 / At1g20510 / ▲ / ▲ / Metabolisms
Os09g0440300 / Similar to Aldehyde dehydrogenase family 7 member A1 / 2.1 / 1.1 / 1.0 / 0.8 / 1.0 / 1.4 / 0.9 / 1.0 / 1.0 / 1.0 / At1g54100 / ▲▼ / ▲ / ▲ / ▲
Os04g0543900 / Similar to Glutamate dehydrogenase (EC 1.4.1.3) (GDH). / 6.2 / 1.4 / 1.5 / 0.7 / 0.9 / 1.2 / 1.1 / 1.1 / 1.0 / 1.1 / At5g07440 / ▲▼ / ▲
Os06g0289200 / UDP-glucuronosyl/UDP-glucosyltransferase family protein. / 3.4 / 1.1 / 0.9 / 1.1 / 1.0 / 0.9 / 1.1 / 1.0 / 1.0 / 1.0 / At3g50740 / ▲
Os01g0597800 / UDP-glucuronosyl/UDP-glucosyltransferase family protein. / 3.1 / 1.1 / 1.1 / 1.1 / 1.0 / 1.0 / 1.0 / 1.0 / 1.0 / 1.0 / At2g15490 / ▲ / ▲
Os01g0638000 / UDP-glucuronosyl/UDP-glucosyltransferase family protein. / 9.4 / 4.5 / 3.9 / 3.2 / 1.0 / 0.9 / 0.9 / 1.0 / 1.0 / 1.3
Os04g0556400 / Similar to Cis-zeatin O-glucosyltransferase 1 (EC 2.4.1.215) (cisZOG1). / 3.5 / 1.2 / 1.3 / 1.4 / 0.8 / 1.6 / 1.1 / 1.2 / 0.9 / 1.6
Os04g0565200 / Similar to Cis-zeatin O-glucosyltransferase 1 (EC 2.4.1.215) (cisZOG1). / 5.1 / 0.7 / 1.1 / 1.2 / 0.5 / 0.9 / 1.0 / 1.0 / 1.0 / 1.1
Os10g0517500* / Cys/Met metabolism pyridoxal-phosphate-dependent enzymes family protein. / 37.6 / 1.7 / 1.6 / 1.2 / 0.8 / 1.4 / 1.0 / 1.2 / 1.6 / 1.2 / At1g64660 / ▲ / ▲
Os11g0566800 / Similar to Bibenzyl synthase (EC 2.3.1.-). / 0.3 / 1.0 / 0.6 / 0.8 / 0.8 / 0.9 / 1.1 / 0.9 / 1.3 / 0.6 / At5g13930 / ▼ / ▲
Os03g0347900 / Terpenoid cylases/protein prenyltransferase alpha-alpha toroid domain containing protein. / 0.4 / 0.3 / 1.6 / 1.3 / 0.8 / 1.3 / 0.8 / 0.8 / 0.9 / 1.3 / At5g23960 / ▼
Os04g0581000 / Similar to Flavanone 3-hydroxylase-like protein. / 1.0 / 2.8 / 2.7 / 1.7 / 1.8 / 1.6 / 1.1 / 1.1 / 0.8 / 1.0 / At5g24530 / ▲ / ▲ / Phenylpropanoid metabolisms
Os03g0122300 / Similar to Flavanone 3-hydroxylase-like protein. / 2.7 / 2.1 / 1.5 / 1.2 / 1.3 / 1.7 / 1.0 / 1.0 / 1.0 / 1.2
Os12g0189300 / Isocitrate lyase and phosphorylmutase family protein. / 4.9 / 0.9 / 0.5 / 1.6 / 2.2 / 0.6 / 0.8 / 0.8 / 1.2 / 0.6 / At1g77060 / No data / ▲ / Carbohydrate metabolism
Os05g0595100 / Similar to UDPglucose 4-epimerase-like protein. / 2.9 / 1.2 / 1.1 / 1.1 / 1.2 / 0.8 / 0.9 / 1.1 / 1.0 / 1.1 / None / ▲ / ▲ / ▲ / ▲
Os02g0661100 / Similar to Trehalose-6-phosphate phosphatase. / 5.2 / 1.2 / 0.9 / 0.8 / 0.8 / 0.7 / 0.9 / 0.8 / 1.1 / 0.6 / At5g51460 / No data / ▲ / ▲
Os07g0637300 / Similar to Pyruvate dehydrogenase kinase isoform 2. / 2.3 / 1.2 / 1.3 / 0.8 / 0.8 / 0.3 / 1.4 / 1.1 / 1.2 / 1.0 / At3g06483 / No change / ▲ / ▲ / ▲ / ▲
Os02g0168100 / Similar to 4-hydroxyphenylpyruvate dioxygenase / 5.4 / 2.1 / 1.3 / 1.3 / 1.0 / 0.5 / 1.1 / 1.1 / 1.0 / 1.0 / At1g06570 / No change / ▲ / ▲ / ▲ / Amino acid metabolism
Os02g0611200 / Similar to S-adenosylmethionine decarboxylase proenzyme / 4.2 / 1.2 / 1.2 / 1.1 / 1.0 / 0.8 / 1.2 / 1.1 / 1.1 / 1.1 / At3g25570 / No data / ▲ / ▲
Os12g0448900* / Similar to Pathogen-inducible alpha-dioxygenase. / 11.3 / 2.1 / 1.8 / 0.5 / 0.6 / 1.5 / 1.0 / 1.1 / 0.9 / 1.3 / At3g01420 / ▲ / JA biosynthesis
Os03g0225900* / Allene oxide synthase. / 17.2 / 2.2 / 1.8 / 1.1 / 1.1 / 1.1 / 1.3 / 1.2 / 0.9 / 1.2 / At5g42650 / ▲
Os03g0767000 / Similar to Allene oxide synthase (EC 4.2.1.92). / 7.7 / 1.2 / 1.0 / 1.0 / 0.8 / 1.2 / 1.1 / 1.0 / 1.0 / 0.8
Os01g0940800 / Similar to Beta-1,3-glucanase precursor. / 2.1 / 4.0 / 1.4 / 1.2 / 1.8 / 1.8 / 1.7 / 0.9 / 0.4 / 2.6 / At3g57260 / ▲ / ▲ / Glucanase
Os01g0942300 / Similar to Beta glucanase precursor (EC 3.2.1.73) (Fragment). / 1.4 / 2.5 / 2.0 / 1.1 / 0.7 / 0.7 / 1.1 / 1.1 / 1.2 / 0.6
Os01g0944900 / Similar to Beta-1,3-glucanase precursor. / 4.7 / 2.3 / 2.5 / 1.4 / 1.5 / 0.9 / 1.1 / 1.1 / 1.0 / 1.0
Os01g0947000* / Similar to Beta-1,3-glucanase precursor. / 12.2 / 3.8 / 4.3 / 1.8 / 1.9 / 0.9 / 1.0 / 1.1 / 1.0 / 1.2
Os01g0946600 / Similar to Glucan endo-1,3-beta-glucosidase GV (EC 3.2.1.39) ((1->3)-beta-glucan endohydrolase GV) ((1->3)-beta-glucanase isoenzyme GV) (Beta-1,3- endoglucanase GV). / 5.2 / 4.1 / 5.5 / 1.7 / 2.2 / 0.6 / 0.8 / 1.3 / 1.5 / 0.6
Os03g0743900 / Similar to ATP sulfurylase. / 2.7 / 1.1 / 1.2 / 0.8 / 0.7 / 1.1 / 1.0 / 0.9 / 1.1 / 0.8 / At3g22890 / ▲ / Sulfur and GSH metabolisms
Os07g0605800* / Similar to STF-1 (Fragment). / 19.9 / 4.8 / 3.2 / 2.2 / 0.8 / 1.3 / 0.9 / 1.1 / 0.8 / 1.6 / At2g03760 / ▲
Os01g0950000 / Similar to Glutathione S-transferase GST 28 (EC 2.5.1.18) (Fragment). / 5.4 / 3.4 / 2.9 / 2.3 / 1.1 / 1.1 / 1.0 / 0.9 / 0.9 / 1.2 / At3g09270 / ▲
Os09g0240500 / Similar to Sulfate transporter 4.1, chloroplast precursor (AST82). / 2.2 / 1.2 / 1.4 / 1.0 / 1.0 / 0.6 / 1.0 / 0.9 / 1.1 / 0.9 / At5g13550 / ▲ / ▲
Os10g0529300 / Similar to Tau class GST protein 3. / 0.4 / 1.6 / 1.1 / 1.0 / 1.0 / 1.2 / 1.1 / 0.9 / 1.0 / 0.9 / At1g53680 / ▼
Os06g0698300 / Protein phosphatase 2C family protein. / 2.7 / 1.0 / 0.9 / 1.1 / 1.1 / 1.1 / 1.0 / 1.0 / 1.0 / 1.0 / At4g31750 / No data / ▲ / ▲ / ▲ / Protein modification
Os01g0138400 / Protein kinase-like domain containing protein. / 5.2 / 2.2 / 1.7 / 1.3 / 1.1 / 1.1 / 1.1 / 1.1 / 0.7 / 1.3 / At1g67000 / ▲ / ▲
Os03g0225100 / Protein kinase-like domain containing protein. / 3.7 / 1.1 / 1.1 / 1.0 / 1.1 / 1.0 / 1.1 / 1.1 / 1.1 / 1.3 / At1g73500 / ▲ / ▲
Os01g0884300 / OsNAC6 / 3.0 / 1.1 / 1.3 / 1.0 / 1.2 / 1.3 / 1.0 / 1.2 / 1.1 / 1.0 / At1g01720 / No data / ▲ / ▲ / ▲ / ▲ / Transcription factor
Os02g0181300 / WRKY transcription factor 71 (Transcription factor WRKY09). / 17.5 / 3.2 / 1.7 / 0.9 / 1.0 / 1.5 / 1.1 / 1.1 / 0.7 / 1.0 / None / ▲ / ▲ / ▲
Os05g0129300 / Basic/leucine zipper protein. (lip19) / 3.9 / 1.1 / 1.1 / 0.8 / 0.9 / 0.9 / 0.9 / 1.0 / 1.2 / 0.8 / At3g62420 / No data / ▲
Os03g0764100 / Zinc finger transcription factor ZF1. / 6.5 / 1.8 / 1.3 / 0.9 / 0.9 / 1.2 / 0.9 / 1.1 / 0.9 / 1.1 / At5g04340 / No change / ▲
Os01g0165000 / DRE binding protein 2. / 2.4 / 1.1 / 1.2 / 1.0 / 0.9 / 1.3 / 1.2 / 1.1 / 1.0 / 1.1 / At5g05410 / ▲
Os02g0624300 / Similar to Y19 protein. / 8.7 / 2.3 / 1.6 / 1.1 / 1.2 / 1.8 / 0.9 / 1.0 / 0.7 / 1.1 / At3g23250 / ▲
Os03g0815100* / Similar to OsNAC6 protein. / 10.9 / 2.3 / 1.6 / 1.0 / 1.1 / 2.3 / 0.8 / 1.1 / 0.9 / 1.2 / At5g63790 / ▲ / ▲
Os03g0820300* / Similar to ZPT2-14. / 43.3 / 16.0 / 3.5 / 1.4 / 1.3 / 1.0 / 0.9 / 1.0 / 1.0 / 1.0 / At5g59820 / ▲
Os03g0860100 / Similar to Ethylene-responsive transcription factor 2 (Ethylene-responsive element binding factor 2) (EREBP-2) (AtERF2). / 12.5 / 2.7 / 2.5 / 1.3 / 0.8 / 2.0 / 1.0 / 0.8 / 0.9 / 0.9 / At3g23240 / ▲
Os03g0437200* / Zinc finger, C2H2-type domain containing protein. / 28.0 / 2.8 / 1.5 / 0.9 / 0.7 / 1.9 / 1.2 / 1.2 / 0.6 / 1.4 / At1g27730 / ▲ / Zinc-finger domain containing protein
Os01g0159300 / Zinc finger, RING-type domain containing protein. / 4.2 / 1.2 / 1.4 / 1.0 / 1.0 / 1.0 / 0.9 / 1.1 / 1.0 / 1.0 / At2g37150 / No data / ▲ / ▲ / ▲
Os07g0615200 / ZIM domain containing protein, similar to AtJAZ1. / 4.1 / 1.5 / 1.5 / 1.1 / 0.8 / 1.3 / 1.1 / 1.1 / 1.1 / 0.9 / At1g19180 / ▲ / ▲
Os09g0439200 / ZIM domain containing protein, similar to AtJAZ1. / 7.0 / 1.6 / 1.7 / 1.2 / 0.7 / 0.9 / 1.5 / 1.3 / 1.2 / 1.1
Os01g0135900 / Similar to Cytosolic class I small heat shock protein 3C (Fragment). / 2.2 / 1.6 / 1.6 / 1.3 / 1.1 / 1.1 / 1.2 / 1.0 / 1.1 / 1.0 / At3g46230 / ▲ / ▲ / Heat shock protein
Os03g0267200 / Low molecular mass heat shock protein Oshsp17.7. / 9.8 / 3.0 / 1.2 / 1.2 / 1.2 / 1.0 / 1.0 / 1.0 / 1.0 / 0.9
Os02g0758000 / Similar to Low molecular weight heat shock protein precursor (Mitochondrial small heat shock protein 22). / 23.8 / 4.8 / 2.0 / 0.7 / 0.9 / 1.3 / 0.7 / 0.9 / 0.8 / 1.1 / At5g51440 / ▲ / ▲
Os06g0253100 / HSP20-like chaperone domain containing protein. / 3.5 / 2.4 / 1.4 / 1.2 / 1.2 / 1.4 / 0.6 / 0.9 / 0.8 / 1.1 / At5g37670 / ▲
Os12g0181600 / Amino acid/polyamine transporter II family protein. / 6.8 / 3.2 / 1.4 / 1.3 / 1.2 / 1.1 / 1.1 / 0.8 / 1.0 / 1.0 / At1g77380 / ▲ / Transport
Os12g0431100 / AAA ATPase domain containing protein. / 0.6 / 1.0 / 1.1 / 0.8 / 0.6 / 2.2 / 0.9 / 0.9 / 0.8 / 0.9 / At3g28510 / ▲ / ▲
Os01g0124400 / Similar to Bowman Birk trypsin inhibitor. / 5.2 / 4.9 / 4.0 / 1.4 / 2.2 / 1.3 / 1.1 / 1.1 / 0.5 / 1.7 / None / ▲ / ▲ / ▲ / Others
Os07g0468200 / Hypothetical protein. / 10.3 / 2.7 / 2.3 / 1.6 / 1.0 / 1.1 / 1.0 / 1.2 / 1.1 / 1.2 / None / ▲ / ▲
Os03g0701200 / Similar to Sugar-starvation induced protein (Fragment). / 2.5 / 1.0 / 1.3 / 0.9 / 0.9 / 1.1 / 0.7 / 0.9 / 1.0 / 0.7 / At2g32150 / ▲ / ▲ / ▲ / ▲ / ▲ / ▲
Os01g0186900 / WSI724 / 9.8 / 2.2 / 1.1 / 0.7 / 0.9 / 1.1 / 0.7 / 1.1 / 1.0 / 1.2 / At5g12010 / No data / ▲ / ▲ / ▲ / ▲
Os02g0756800 / Phosphate-induced protein 1 conserved region family protein. / 2.9 / 1.1 / 1.2 / 0.8 / 1.0 / 1.3 / 0.7 / 1.1 / 1.0 / 1.0 / At4g08950 / No data / ▲ / ▲ / ▲
Os01g0570800 / IQ calmodulin-binding region domain containing protein. / 3.1 / 1.1 / 1.2 / 0.9 / 1.1 / 1.1 / 0.9 / 1.0 / 0.9 / 1.1 / At2g26190 / No data / ▲
Os05g0506000 / Similar to MS5-like protein (Fragment). / 4.9 / 1.4 / 1.3 / 0.8 / 1.0 / 0.4 / 0.6 / 0.8 / 0.9 / 1.0 / At1g04770 / ▲
Os07g0638100 / TolB, C-terminal domain containing protein. / 17.6 / 7.6 / 7.3 / 3.0 / 1.9 / 2.7 / 0.9 / 1.1 / 1.0 / 1.7 / At4g01870 / ▲
Os07g0643800 / Similar to Dirigent protein. / 4.7 / 4.1 / 2.2 / 1.8 / 0.9 / 1.1 / 1.6 / 1.3 / 0.7 / 2.4 / At1g64160 / ▲
Os02g0689900 / TGF-beta receptor, type I/II extracellular region family protein. / 0.4 / 0.8 / 1.2 / 1.1 / 1.2 / 0.5 / 1.1 / 1.1 / 1.5 / 1.3 / At1g32450 / ▼
1Fold change values greater than 2 or less than 0.5 are indicated in bold letters.
2Red and green triangles indicate up- or down-regulation, respectively.
*Induction by Cd was confirmed by semi-quantitative reverse transcription PCR (Supplemental Fig. 1).