Table1: The NCBI accession numbers of 54 endonuclease III protein homologs with their carrier organism as well as the length of the protein sequences is tabulated
S. No. / Organism name / Accession no.(Protein sequence) / Name of homolog(as annotated by database) / Protein sequence length
Ashbyagossypii ATCC 10895 / NP_983680.1 / ACR278Wp (Endonuclease III) / 367
Aeromonassalmonicida subsp. salmonicida A449 / YP_001142271.1 / Endonuclease III / 213
Arthrobacterchlorophenolicus A6 / YP_002489216.1 / Endonuclease III / 291
Borreliarecurrentis A1 / YP_002223188.1 / Endonuclease III / 205
Chlamydia muridarum str. Nigg / NP_296453.1 / Endonuclease III / 210
Chlorobiumtepidum TLS / NP_662592.1 / Endonuclease III / 213
Deinococcusradiodurans R1 / NP_294012.1 / Endonuclease III / 225
Dictyoglomusturgidum DSM 6724 / YP_002352582.1 / Endonuclease III / 210
Schizosaccharomycespombe 972h- / NP_593210.1 / Endonuclease III / 355
ElusimicrobiumminutumPei191 / YP_001875052.1 / Endonuclease III / 215
Arabidopsis thaliana / CAC16135.1 / Endonuclease III / 354
Chloroherpetonthalassium ATCC 35110 / YP_001995475.1 / Endonuclease III / 213
Halogeometricumborinquense DSM 11551 / ZP_03997896.1 / Endonuclease III / 227
Halorhodospirahalophila / YP_001002308.1 / Endonuclease III / 213
Oryza sativa / AAX96284.1 / Endonuclease III / 373
Sulfurihydrogenibium sp. YO3AOP1 / YP_001931649.1 / Endonuclease III / 209
Entamoebadispar / XP_001733691.1 / Endonuclease III / 241
Escherichia coli str. K-12 substr. MG1655 / NP_416150.1 / Endonuclease III / 211
GemellahaemolysansATCC 10379 / ZP_04777149.1 / Endonuclease III / 214
GemmatimonasaurantiacaT-27 / YP_002760336.1 / Adenine glycosylase / 221
GramellaforsetiiKT0803 / YP_861821.1 / Endonuclease III / 218
Penicilliummarneffei ATCC 18224 / XP_002143355.1 / HhH-GPD family protein / 449
Lentisphaeraaraneosa HTCC2155 / ZP_01872881.1 / Endonuclease III / 212
Thermomicrobiumroseum (MutY) / YP_002524150.1 / MutY / 358
Methanobrevibactersmithii ATCC 35061 / YP_001272845.1 / Endonuclease III / 210
MethylacidiphiluminfernorumV4 / YP_001940005.1 / A/G-specific DNA glycosylase / 355
E.coli (MutY) / 1KG4|A / MutY / 225
Homo sapiens (MutY) / CAI21715.1 / MutY / 291
Nostocpunctiforme PCC 73102 / YP_001867358.1 / Endonuclease III / 229
Caenorhabditiselegans / NP_497859.1 / Nth-1 (Endonuclease III) / 259
Homo sapiens / AAH00391.2 / Nthl1
(Endonuclease III) / 305
Oceanobacillusiheyensis HTE831 / NP_691963.1 / DNA-lyase / 222
Parabacteroidesdistasonis ATCC 8503 / YP_001302528.1 / Endonuclease III / 221
PerkinsusmarinusATCC 50983 / EER15889.1 / Endonuclease III / 292
PetrotogamobilisSJ95 / YP_001568415.1 / Endonuclease III / 210
Planctomycesmaris DSM 8797 / ZP_01852813.1 / Endonuclease III / 240
Rhizobiumleguminosarumbv. trifolii / ZP_02297317.1 / Endonuclease III / 298
Rhodococcuserythropolis PR4 / YP_002763929.1 / Endonuclease III / 261
RoseiflexuscastenholziiDSM 13941 / YP_001432854.1 / DNA-(apurinic or apyrimidinic site) lyase / 219
Saccharophagusdegradans 2-40 / YP_528076.1 / Endonuclease III / 227
SalinibacterruberDSM 13855 / YP_446456.1 / Endonuclease III / 324
SalinisporatropicaCNB-440 / YP_001161125.1 / Endonuclease III / 276
Synechococcussp. WH 8102 / NP_897435.1 / Endonuclease III / 217
Synechocystissp. PCC 6803 / NP_441082.1 / Endonuclease III / 219
Leptospirillum sp. Group II '5-way CG' / EDZ38087.1 / Endonuclease III / 241
Caldivirgamaquilingensis IC-167 / YP_001540481.1 / HhH-GPD family protein / 233
Verrucomicrobiumspinosum / ZP_02928493.1 / Endonuclease III / 217
Acholeplasmalaidlawii PG-8A / YP_001620232.1 / Endonuclease III / 214
Fusobacteriumnucleatum subsp. polymorphum ATCC 10953 / YP_002164365.1 / DNA-(apurinic or apyrimidinic site) lyase / 216
CandidatusSolibacterusitatus Ellin6076 / YP_825039.1 / Endonuclease III / 219
Thermotogalettingae TMO / YP_001471128.1 / Endonuclease III / 217
ThermosinuscarboxydivorNor1 / ZP_01666970.1 / Endonuclease III / 213
Treponemapallidumsubsp. pallidum str. Nichols / NP_219212.1 / Endonuclease III / 211
Arthrobacterchlorophenolicus A6 / YP_002489216.1 / Endonuclease III / 291
Serial No. / Phylum/
Kingdom / Division / Branch color / Symbol with color
1. / Archaea / Euryarchaeota, Crenarchaeota / /
2. 1 / Bacteria / Bacteroidetes / /
2.2 / Chlorobi / /
2.3 / Chlamydiae / /
2.4 / Chloroflexi / /
2.5 / Cyanobacteria / /
2.6 / Deinococcus / /
2.7 / Dictyoglomi / /
2.8 / Elusimicrobia / /
2.9 / Actinobacteria / /
2.10 / Aquificae / /
2.11 / Lentisphaerae / /
2.12 / Nitrospirae / /
2.13 / Planctomycetes / /
2.14 / Spirochaetes / /
2.15 / Firmicutes / /
2.16 / Thermotogae / /
2.17 / Verrucomicrobia / /
2.18 / Proteobacteria / /
2.19 / Gemmatimonadetes / /
2.20 / Tenericutes / /
2.21 / Fusobacteria / /
2.22 / Acidobacteria / /
3 / Eukaryotes / Fungi ,Protista, Plantae, Animalia / /
Table S2: Color codes and symbols used during phylogenetic tree reconstruction. Organisms from the same division are represented with the same symbol and branch color.
Figure 1: NJ tree of endonuclease III homologsof a)Archaea (42)b)Bacteridetes(19) c)Chlorobi(5) d)Chloroflexi (6)e)Cyanobacteria(17) f)Actinobacteria(25) g)Aquificae(5) h)Firmicutes(45) i)Thermotogae(7) j)Verrumicrobia(7) k)Fungi(11) l)Protista(11) m)Plantae(5) n)Animilia (18) . The number of species within each division is mentioned within parenthesis. The bootstrap consensus tree inferred from 1000 replicates. Bootstrap support values are presented next to the tree branches. Species indicated by an arrow are selected for further analysis.
Figure 1a
Fig 1b
Fig 1c
Fig. 1d
Fig. 1e
Fig. 1f
Fig. 1g
Fig. 1h
Fig. 1i
Fig. 1j
Fig. 1k
Fig. 1l
Fig. 1m
Fig. 1n