Analysis of significant probiotic properties of Lactobacillus plantarum CS24.2
L. plantarum CS24.2 was analyzed for selected probiotic properties such as its ability to adhere to Caco-2 cells, its antimicrobial activity against indicator bacteria listed in Table 1 and the ability to tolerate acidic pH and bile salts. The change in the level of transcripts for selected cytokines/chemokine in Caco-2 cells co-cultured with L. plantarum CS24.2 was analysed (Figure 1). The methodologies followed are as described earlier [1].
Table 1. Analysis of probiotic properties of Lactobacillus casei CS24.2.
S.D., Standard deviation; n=3, number of independent experiment.
Adhered cells/Caco-2 n / Acid and bile tolerance cfu/ml (% survival)*Mean ± S.D. / 0 min / pH 2.0 / 3% bile salts
19.3 ± 4.6 / 1.67 × 106 (100%) / 0.79 × 105 (4.73%) / 2.12 × 105 (12.7%)
Antimicrobial assay‡
Shigella dysentery / Staphylococcus aureus / Pseudomonas aeruginosa / E. coli
+++ / ++ / +++ / +++
* survival after 2 hrs incubation in acidic buffer, pH 2.0 and 3% bile salts in MRS broth.
‡ Symbols refer to the size of the growth inhibition halo: ++, from 2 to 5 mm; +++, more than 5 mm.
Figure 1. Cytokines and chemokine transcript level in Caco-2 cells co incubated with L. plantarum CS24.2 (1 × 108 cfu/ml), control to which no bacteria were added. The integrated density values of each cytokine or chemokine are used to express its transcript levels. Each bar represents mean value and standard deviation as error bar. Significant ANOVAs were followed by Dunnett test for multiple comparisons versus control group.†Within each cytokine or chemokine, mean value was significantly higher than that of the control (P<0.05).
Reference
1. Gaudana SB, Dhanani AS, Bagchi T (2010) Probiotic attributes ofLactobacillusstrains isolated from food and human origin. Br J Nutr 103: 1620-1628
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