Table 3 - Descriptive Statistical Analysis of the Candidate Genes in Mouse Hemibrain At

Table 1 supplementary - Housekeeping genes used for gene expression studies on mouse brain regions

Table 2 supplementary - Descriptive statistical analysis of the candidate genes in mouse hemibrain at P7 and at 6M

Table 3 supplementary -Descriptive statistical analysis of the candidate genes in mouse cerebellum at P7 and at 6M

Table 4 supplementary -Descriptive statistical analysis of the candidate genes in mouse brain stem at P7 and at 6M

Table 5 supplementary -Descriptive statistical analysis of the candidate genes in mouse hippocampus at P7 and at 6M

Table 6 supplementary -Descriptive statistical analysis of the candidate genes in mouse medial septum at P7 and at 6M

Table 7 supplementary -Descriptive statistical analysis of the candidate genes in mouse frontal neocortex at postnatal day 7 and at 6 months of age

Table 8 supplementary -Descriptive statistical analysis of the candidate genes in mouse olfactory bulb at P7 and at 6M

Table 9a supplementary -Comparison of the six candidate reference genes in mouse hemibrain at P7

Table 9b supplementary -Comparison of the six candidate reference genes in mouse hemibrain at 6M

Table 9c supplementary -Candidate reference genes in mouse hemibrain (P7 and 6M considered together)

Table 10a - Comparison of the six candidate reference genes in mouse cerebellum at P7.

Table 10b - Comparison of the six candidate reference genes in mouse cerebellum at 6M.

Table 10c supplementary - Candidate reference genes and the best combination of two genes in mouse cerebellum (P7 and 6M considered together)

Table 11a supplementary - Comparison of the six candidate reference genes in mouse brain stem at P7

Table 11b supplementary - Comparison of the six candidate reference genes in mouse brain stemat 6M

Table 11c supplementary - Candidate reference genes and the best combination of two genes in mouse brain stem (P7 and 6M considered together)

Table 12a supplementary - Comparison of the six candidate reference genes in mouse hippocampus at P7

Table 12b supplementary - Comparison of the six candidate reference genes in mouse hippocampus at 6M

Table 12c supplementary - Candidate reference genes and the best combination of two genes in mouse hippocampus (P7 and 6M considered together)

Table 13a supplementary - Comparison of the six candidate reference genes in mouse medial septum at P7

Table 13b supplementary - Comparison of the six candidate reference genes in mouse medial septum at 6M

Table 13c supplementary - Candidate reference genes and the best combination of two genes in mouse medial septum (P7 and 6M considered together)

Table 14asupplementary - Comparison of the six candidate reference genes in mouse frontal neocortex at P7

Table 14bsupplementary - Comparison of the six candidate reference genes in mouse frontal neocortex at 6M

Table 14csupplementary - Candidate reference genes and the best combination of two genes in mouse frontal neocortex (P7 and 6M considered together)

Table 15asupplementary - Comparison of the six candidate reference genes in mouse olfactory bulb at P7

Table 15bsupplementary - Comparison of the six candidate reference genes in mouse olfactory bulb at6M

Table 15csupplementary - Candidate reference genes and the best combination of two genes in mouse olfactory bulb (P7 and 6M considered together)

Table 16asupplementary - Candidate reference genes in mouse brain at P7 (global analysis of the six brain regions)

Table 16bsupplementary - Candidate reference genes in mouse brain at 6M (global analysis of the six brain regions)

Table 16csupplementary - Candidate reference genes in mouse brain (P7 and 6M considered together. global analysis of the six brain regions)

Table supplementary 17 - Pgk1 expression in mouse brain regions

Table 1 supplementary - Housekeeping genes used for gene expression studies on mouse brain regions

Results from a PubMed search concerning the use of housekeeping genes in gene expression studies performed on different mouse brain regions. We had access to 101 articles from November 2007 to December 2006 for brain, to 70 articles from October 2007 to December 2004 for cerebellum, to 68 articles from September 2007 to December 2004 for brain stem, to 76 articles from October 2007to December 2004 for hippocampus, to 12 articles from June 2007 to May 1999 for septum, to 18 articles from December 2006 to May 1997 for neocortex and to 36 articles fromNovember 2007 to December 1994 for olfactory bulb. Each cell of the table contains the percentage of articles citing a certain reference gene, calculated on the number of all considered articles. It is notable that the whole number of the citations exceeded the number of the considered articles for each tissue, as some of them used more than one housekeeping gene.

Table 2 supplementary -Descriptive statistical analysis of the candidate genes in mouse hemibrain at P7 and at 6M

Abbreviations: CT: cycle threshold; Avg 2-CT: average 2–CT values; Max 2-CT and Min 2-CT: extreme values of 2–CT ; Sd: Standard deviation.

Table 3 supplementary -Descriptive statistical analysis of the candidate genes in mouse cerebellum at P7 and at 6M

Abbreviations: CT: cycle threshold; Avg 2-CT: average 2–CT values; Max 2-CT and Min 2-CT: extreme values of 2–CT ; Sd: Standard deviation.

Table 4 supplementary -Descriptive statistical analysis of the candidate genes in mouse brain stem at P7 and at 6M

Abbreviations: CT: cycle threshold; Avg 2-CT: average 2–CT values; Max 2-CT and Min 2-CT: extreme values of 2–CT ; Sd: Standard deviation.

Table 5 supplementary -Descriptive statistical analysis of the candidate genes in mouse hippocampus at P7 and at 6M

Abbreviations: CT: cycle threshold; Avg 2-CT: average 2–CT values; Max 2-CT and Min 2-CT: extreme values of 2–CT ; Sd: Standard deviation.

Table 6 supplementary -Descriptive statistical analysis of the candidate genes in mouse medial septum at P7 and at 6M

Abbreviations: CT: cycle threshold; Avg 2-CT: average 2–CT values; Max 2-CT and Min 2-CT: extreme values of 2–CT ; Sd: Standard deviation.

Table 7 supplementary -Descriptive statistical analysis of the candidate genes in mouse frontal neocortex at P7 and at 6M

Abbreviations: CT: cycle threshold; Avg 2-CT: average 2–CT values; Max 2-CT and Min 2-CT: extreme values of 2–CT ; Sd: Standard deviation.

Table 8 supplementary -Descriptive statistical analysis of the candidate genes in mouse olfactory bulb at P7 and at 6M

Abbreviations: CT: cycle threshold; Avg 2-CT: average 2–CT values; Max 2-CT and Min 2-CT: extreme values of 2–CT ; Sd: Standard deviation.

Table 9a. 9b and 9c supplementary -Comparison of the six candidate reference genes in mouse hemibrain at P7. at 6M and at P7 versus 6M

Genes were listed according to their coefficient of variation (CV. defined as the ratio of the standard deviation to the average 2-CT) and to their stability values calculated by NormFinder and GeNorm softwares.Results obtained from P7 and 6M samples were analyzed first separately, as two independent groups. Secondly. results obtained from P7 and 6M samples were analyzed together, as a unique experimental group for the CV and GeNorm methods. On the contrary, the NormFinder analysis considered the two ages as two subgroups of a single experimental group. a.bHigh expression stability is indicated by a low stability value.

Table 10a. 10b and 10c supplementary -Comparison of the six candidate reference genes in mouse cerebellum at P7. at 6M and at P7 versus 6M

Genes were listed according to their coefficient of variation (CV. defined as the ratio of the standard deviation to the average 2-CT) and to their stability values calculated by NormFinder and GeNorm softwares.Results obtained from P7 and 6M samples were analyzed first separately, as two independent groups. Secondly. results obtained from P7 and 6M samples were analyzed together, as a unique experimental group for the CV and GeNorm methods. On the contrary, the NormFinder analysis considered the two ages as two subgroups of a single experimental group. a.bHigh expression stability is indicated by a low stability value.

Table 11a. 11b and 11c supplementary -Comparison of the six candidate reference genes in mouse brainstem at P7. at 6M and at P7 versus 6M

Genes were listed according to their coefficient of variation (CV. defined as the ratio of the standard deviation to the average 2-CT) and to their stability values calculated by NormFinder and GeNorm softwares.Results obtained from P7 and 6M samples were analyzed first separately, as two independent groups. Secondly. results obtained from P7 and 6M samples were analyzed together, as a unique experimental group for the CV and GeNorm methods. On the contrary, the NormFinder analysis considered the two ages as two subgroups of a single experimental group. a.bHigh expression stability is indicated by a low stability value.

Table 12a. 12b and 12c supplementary -Comparison of the six candidate reference genes in mouse hippocampus at P7. at 6M and at P7 versus 6M

Genes were listed according to their coefficient of variation (CV. defined as the ratio of the standard deviation to the average 2-CT) and to their stability values calculated by NormFinder and GeNorm softwares.Results obtained from P7 and 6M samples were analyzed first separately, as two independent groups. Secondly. results obtained from P7 and 6M samples were analyzed together, as a unique experimental group for the CV and GeNorm methods. On the contrary, the NormFinder analysis considered the two ages as two subgroups of a single experimental group. a.bHigh expression stability is indicated by a low stability value.

Table 13a. 13b and 13c supplementary -Comparison of the six candidate reference genes in mouse medial septum at P7. at 6M and at P7 versus 6M

Genes were listed according to their coefficient of variation (CV. defined as the ratio of the standard deviation to the average 2-CT) and to their stability values calculated by NormFinder and GeNorm softwares.Results obtained from P7 and 6M samples were analyzed first separately, as two independent groups. Secondly. results obtained from P7 and 6M samples were analyzed together, as a unique experimental group for the CV and GeNorm methods. On the contrary, the NormFinder analysis considered the two ages as two subgroups of a single experimental group. a.bHigh expression stability is indicated by a low stability value.

Table 14a. 14b and 14c supplementary -Comparison of the six candidate reference genes in mouse frontal neocortex at P7. at 6M and at P7 versus 6M

Genes were listed according to their coefficient of variation (CV. defined as the ratio of the standard deviation to the average 2-CT) and to their stability values calculated by NormFinder and GeNorm softwares.Results obtained from P7 and 6M samples were analyzed first separately, as two independent groups. Secondly. results obtained from P7 and 6M samples were analyzed together, as a unique experimental group for the CV and GeNorm methods. On the contrary, the NormFinder analysis considered the two ages as two subgroups of a single experimental group. a.bHigh expression stability is indicated by a low stability value.

Table 15a. 15b and 15c supplementary -Comparison of the six candidate reference genes in mouse olfactory bulb at P7. at 6M and at P7 versus 6M

Genes were listed according to their coefficient of variation (CV. defined as the ratio of the standard deviation to the average 2-CT) and to their stability values calculated by NormFinder and GeNorm softwares.Results obtained from P7 and 6M samples were analyzed first separately, as two independent groups. Secondly. results obtained from P7 and 6M samples were analyzed together, as a unique experimental group for the CV and GeNorm methods. On the contrary, the NormFinder analysis considered the two ages as two subgroups of a single experimental group. a.bHigh expression stability is indicated by a low stability value.

Table 16a. 16b and 16c supplementary -Comparison of the six candidate reference genes in mouse brain (global analysis of the six regions) at P7. at 6M and at P7 versus 6M

Genes were listed according to their coefficient of variation (CV. defined as the ratio of the standard deviation to the average 2-CT) and to their stability values calculated by NormFinder and GeNorm softwares.Results obtained from all P7 and 6M samples were analyzed first separately, as two independent groups. In this first analysis, the CV and GeNorm methods considered the data from each age group as a unique experimental group. On the contrary, the NormFinder analysis considered the different brain regions as six subgroups of a single experimental group. Secondly. results obtained from all P7 and 6M samples were analyzed together. In this second data analysis, Normfinder considered the two ages as two subgroups of a single experimental group, while the CV and GeNorm methods considered all the samples as a unique experimental group.

a.bHigh expression stability is indicated by a low stability value.