Supplemental Table 1. Surveillance of Aquatic Animal Viruses from Cultured Salmonids In

Supplemental Table 1. Surveillance of Aquatic Animal Viruses from Cultured Salmonids In

Supplemental table 1. Surveillance of aquatic animal viruses from cultured salmonids in Korea between February 2013 and April 2015

Sampling sites
(Farms) / Month/year / Species / Environmentalcondition / No. of fish / Size / Clinical sign / Virus
(Cultured on EPC cell line)
Temperature
(℃) / DO
(mg/L) / pH / Salinity
(‰) / (Means±SD cm)
Chuncheon
CcA / Mar-14 / rainbow trout / 13.7 / 7.7 / 8.0 / 0.1 / 20 / 5.6±0.4 / - / -
Oct-14 / rainbow trout / 16.9 / 9.3 / 8.6 / 0.1 / 8 / 22.7±2.0 / gill hyperemia / -
Mar-15 / rainbow trout / 10.8 / 11.8 / 8.1 / 0.1 / 30 / 8.3±0.7 / - / -
CcB / Mar-14 / rainbow trout / 10.6 / 9.2 / 7.8 / 0.1 / 20 / 10.2±1.1 / gill anemia, mucus hypersecretion / -
Oct-14 / rainbow trout / 16.0 / 8.9 / 8.1 / 0.1 / 12 / 24.1±5.1 / gill congestion, mucus hypersecretion
Mar-15 / rainbow trout / 7.1 / 12.4 / 8.0 / 0.2 / 40 / 12.9±1.4 / -
CcC / Mar-15 / rainbow trout / 15.0 / 11.2 / 8.9 / 0.1 / 30 / 10.6±0.8 / -
Gangneung
GnA / Mar-14 / rainbow trout / 14.6 / 9.2 / 8.4 / 0.2 / 10 / 31.4±3.6 / - / -
Oct-14 / rainbow trout / 13.5 / 10.0 / 8.3 / 0.1 / 8 / 27.5±7.2 / gill hyperemia, mucus hypersecretion / -
GnB / Mar-14 / rainbow trout / 10.8 / 10.7 / 8.5 / 0.1 / 8 / 19.7±6.9 / gill congestion, mucus hypersecretion / IHNV
(+ve)
Hwacheon
HcA / Jun-13 / masu salmon / NTa / NT / NT / NT / 6 / 10.6±1.5 / gill anemia / IHNV
(-ve)
Jun-14 / masu salmon / NT / NT / NT / NT / 14 / 12.5±1.5 / - / -
Apr-15 / masu salmon / 10.9 / 17.1 / 8.1 / 0.1 / 44 / 8.5±1.1 / - / -
HcB / Apr-15 / masu salmon / 11.3 / 20.2 / 8.3 / 0.1 / 38 / 9.6±0.7 / mucus hypersecretion
Jeongseon
JS / Feb-15 / rainbow trout / 12.8 / 10.8 / 8.4 / 0.3 / 20 / 11.3±1.1 / - / -
Pyeongchang
PcA / Mar-13 / rainbow trout / 11.3 / 10.9 / 8.6 / 0.2 / 12 / 13.2±1.4 / gill hyperemia
Apr-13 / rainbow trout / NT / NT / NT / NT / 20 / 13.9±2.6 / gill hemorrhage / IHNV
(-ve)
Aug-13 / rainbow trout / 14.0 / 11.0 / 8.8 / 0.7 / 10 / 20.1±0.9 / mucus hypersecretion
Nov-13 / rainbow trout / 11.5 / 14.3 / 8.1 / 0.2 / 11 / 16.1±2.1 / gill hemorrhage
Feb-14 / rainbow trout / 12.2 / 10.3 / 8.8 / 0.2 / 20 / 7.8±5.8 / gill anemia, necrosis of pectoral fin
Sep-14 / rainbow trout / 13.8 / 12.5 / 8.2 / 0.2 / 30 / 8.5±5.5 / gill hyperemia, mucus hypersecretion
Feb-15 / rainbow trout / 9.4 / 9.4 / 8.4 / 0.2 / 20 / 20.1±1.7 / mucus hypersecretion / -
PcB / Mar-13 / rainbow trout / 11.2 / 12.0 / 8.5 / 0.1 / 11 / 22.7±2.3 / mucus hypersecretion
May-13 / rainbow trout / 12.5 / 12.9 / 8.8 / 0.1 / 13 / 17.3±2.1 / gill hyperemia
Aug-13 / rainbow trout / 13.0 / 9.1 / 8.8 / 0.2 / 4 / 23.2±3.2 / gill hyperemia
Nov-13 / rainbow trout / 10.9 / 7.6 / 8.1 / 0.1 / 7 / 12.5±1.4 / gill hyperemia
PcC / Mar-13 / rainbow trout / 9.0 / 13.0 / 8.2 / 0.1 / 8 / 22.3±1.1 / - / -
Mar-13 / rainbow trout / 8.8 / 12.8 / 8.3 / 0.1 / 20 / 7.4±0.8 / - / -
May-13 / rainbow trout / 11.5 / 12.8 / 8.8 / 0.1 / 10 / 10.5±1.3 / mucus hypersecretion
Aug-13 / rainbow trout / 15.8 / 10.4 / 8.3 / 0.1 / 20 / 8.5±1.2 / gill anemia
Nov-13 / rainbow trout / 13.2 / 14.7 / 8.1 / 0.1 / 15 / 10.6±0.9 / gill anemia
Mar-14 / rainbow trout / 9.9 / 11.5 / 8.2 / 0.1 / 4 / 19.1±0.8 / mucus hypersecretion / IHNV
(+ve)
Oct-14 / rainbow trout / 14.5 / 9.1 / 8.3 / 0.1 / 7 / 29.4±2.1 / gill hyperemia
Feb-15 / rainbow trout / 10.7 / 13.9 / 8.6 / 0.1 / 20 / 10.2±0.8 / -
PcD / Mar-14 / rainbow trout / 14.5 / 9.1 / 8.3 / 0.1 / 9 / 19.4±1.3 / - / -
Oct-14 / rainbow trout / 14.6 / 9.2 / 8.4 / 0.2 / 9 / 19.2±2.0 / gill anemia, mucus hypersecretion
Mar-15 / rainbow trout / 14.5 / 10.8 / 8.2 / 0.2 / 29 / 7.3±1.0 / - / IHNV
(+ve)
PcE / Mar-14 / rainbow trout / 10.1 / 11.3 / 8.3 / 0.2 / 3 / 15.3±0.4 / - / IHNV
(+ve)
Mar-15 / rainbow trout / 9.6 / 12.1 / 8.0 / 0.2 / 30 / 8.3±0.9 / - / -
PcF / Feb-14 / rainbow trout / 12.4 / 10.1 / 8.1 / 0.2 / 20 / 6.2±0.5 / mucus hypersecretion
Oct-14 / rainbow trout / 14.0 / 9.7 / 8.2 / 0.2 / 12 / 18.5±2.9 / gill hyperemia, anemia
Feb-15 / rainbow trout / 9.8 / 10.2 / 8.3 / 0.2 / 20 / 13.3±2.5 / gill congestion, mucus hypersecretion
PcG / Mar-14 / rainbow trout / NT / NT / NT / NT / 20 / 13.0±1.6 / - / IHNV
(+ve)
Jun-14 / rainbow trout / NT / NT / NT / NT / 13 / 14.7±6.2 / gill anemia / IHNV
(+ve)
PcH / Feb-14 / rainbow trout / 12.2 / 8.7 / 8.2 / 0.2 / 20 / 3.4±0.3 / exophthalmos (pop-eye), gill anemia
Oct-14 / rainbow trout / 13.7 / 7.3 / 8.3 / 0.2 / 11 / 21.8±3.8 / gill hyperemia
Feb-15 / rainbow trout / 7.8 / 12.0 / 8.8 / 0.2 / 40 / 9.0±0.8 / - / IHNV
(-ve)
PcI / Mar-14 / rainbow trout / 10.6 / 9.2 / 7.8 / 0.1 / 20 / 7.8±0.8 / mucus hypersecretion
Oct-14 / rainbow trout / 12.4 / 11.6 / 8.5 / 0.2 / 8 / 18.4±5.1 / gill congestion / -
Yangyang
YyA / Feb-13 / masu salmon / 7.3 / 11.8 / 7.8 / 0.2 / 34 / 3.2±0.2 / mucus hypersecretion / IHNV
(+ve)
May-13 / masu salmon / 11.1 / 12.4 / 8.3 / 0.1 / 20 / 4.3±0.5 / gill hyperemia, mucus hypersecretion
Aug-13 / masu salmon / 20.5 / 10.8 / 8.0 / 0.2 / 20 / 8.0±0.7 / -
Nov-13 / masu salmon / 15.2 / 11.0 / 8.2 / 0.4 / 13 / 11.5±1.7 / gill hyperemia, mucus hypersecretion
Feb-14 / masu salmon / 6.2 / 9.3 / 8.3 / 0.5 / 28 / 3.1±2.6 / - / -
Apr-14 / masu salmon / NT / NT / NT / NT / 25 / 15.1±1.2 / gill hyperemia, mucus hypersecretion / IHNV
(+ve)
Sep-14 / masu salmon / 17.5 / 9.5 / 9.1 / 0.2 / 15 / 14.7±2.1 / gill congestion / -
Jan-15 / masu salmon / 7.3 / 12.2 / 8.1 / 0.2 / 40 / 2.5±0.2 / - / -
Feb-13 / rainbow trout / 7.3 / 12.2 / 7.7 / 0.2 / 10 / 16.2±0.7 / necrosis of pectoral fin
May-13 / rainbow trout / 11.0 / 13.2 / 7.9 / 0.1 / 20 / 4.1±0.3 / mucus hypersecretion
Aug-13 / rainbow trout / 20.5 / 10.4 / 7.8 / 0.2 / 20 / 7.8±0.8 / - / -
Nov-13 / rainbow trout / 15.1 / 10.3 / 8.0 / 0.4 / 13 / 11.3±1.4 / gill anemia / IHNV
(+ve)
Feb-14 / rainbow trout / 6.3 / 9.2 / 8.3 / 0.5 / 10 / 14.2±0.9 / gill congestion / -
Sep-14 / rainbow trout / 17.5 / 8.9 / 8.8 / 0.2 / 15 / 12.1±1.0 / - / -
Jan-15 / rainbow trout / 7.3 / 12.1 / 8.0 / 0.2 / 40 / 3.0±0.3 / - / -
YyB / Jun-13 / masu salmon / NT / NT / NT / NT / 15 / 10.9±2.6 / gill anemia / IHNV
(+ve)
Jun-13 / rainbow trout / 12.5 / 12.3 / 8.6 / 0.0 / 5 / 14.3±0.5 / exophthalmos (pop-eye), gill anemia / IHNV
(+ve)
Jan-15 / rainbow trout / 15.8 / 9.9 / 8.9 / 0.1 / 40 / 9.0±0.8 / mucus hypersecretion
Yeongwol
YwA / Mar-13 / rainbow trout / 12.1 / 10.2 / 8.6 / 0.2 / 16 / 12.7±0.9 / gill hyperemia / -
May-13 / rainbow trout / 12.4 / 11.0 / 8.1 / 0.1 / 10 / 19.1±2.4 / gill hyperemia
Aug-13 / rainbow trout / NT / NT / NT / NT / 4 / 27.4±4.3 / gill anemia
May-14 / rainbow trout / NT / NT / NT / NT / 10 / 9.1±2.3 / gill anemia, mucus hypersecretion / IHNV
(+ve)
Feb-15 / rainbow trout / 9.3 / 12.4 / 8.5 / 0.2 / 20 / 10.6±0.7 / - / IHNV
(+ve)
YwB / Mar-13 / rainbow trout / 7.2 / 9.9 / 8.2 / 0.1 / 5 / 24.7±4.5 / - / -
May-13 / rainbow trout / 11.1 / 10.3 / 8.3 / 0.1 / 25 / 2.4±0.2 / gill anemia / -
Aug-13 / rainbow trout / 16.6 / 9.4 / 8.6 / 0.1 / 12 / 6.6±0.5 / gill anemia
Oct-13 / rainbow trout / 14.9 / 9.5 / 8.9 / 0.1 / 3 / 33.7±4.2 / - / IHNV
(-ve)
YwC / Jun-14 / rainbow trout / NT / NT / NT / NT / 6 / 13.4±1.9 / gill hyperemia
Feb-15 / rainbow trout / 10.7 / 11.7 / 8.5 / 0.1 / 28 / 5.0±0.3 / gill anemia / IHNV
(-ve)
Gumi
GuA / Mar-14 / rainbow trout / 15.7 / 9.0 / 8.5 / 0.2 / 6 / 30.8±2.9 / gill (lamella) hemorrhage
Oct-14 / rainbow trout / 15.9 / 9.1 / 8.4 / 0.2 / 12 / 27.6±4.5 / gill congestion / -
GuB / Mar-14 / rainbow trout / 15.5 / 10.7 / 8.8 / 0.2 / 17 / 6.9±1.0 / gill hemorrhage, congestion / -
Oct-14 / rainbow trout / 14.7 / 10.8 / 8.1 / 0.2 / 6 / 34.8±3.1 / - / -
Supplemental table 2. Primers used to detect aquatic animal virusesfrom salmonids.
Pathogens / Region / Primer / Sequence (5' to 3') / Reference / Manual of Diagnostic Tests for Aquatic Animals, OIE[18]
Epizootic hematopoietic necrosis virus(EHNV) / Major capsid protein (MCP) / M151 / 5'-AACCCGGCTTTCGGGCAGCA-3' / [13] / Chapter 2.3.1
M152 / 5'-CGGGGCGGGGTTGATGAGAT-3'
M153 / 5'-ATGACCGTCGCCCTCATCAC-3'
M154 / 5'-CCATCGAGCCGTTCATGATG-3'
Infectious hematopoietic necrosis virus(IHNV) / Glycoprotein (G) / Forward / 5’-AGAGATCCCTACACCAGAGAC-3’ / [4] / Chapter 2.3.4
Reverse / 5’-GGTGGTGTTGTTTCCGTGCAA-3'
Infectious salmon anemia virus(ISAV) / Genomic segment 8 / ILA1 / 5'-GGCTATCTACCATGAACGAATC-3' / [14] / Chapter 2.3.5
ILA2 / 5'-GCCAAGTGTAAGTAGCACTCC-3'
FA-3 / 5'-GAAGAGTCAGGATGCCAAGACG-3' / [3]
RA-3 / 5'-GAAGTCGATGAACTGCAGCGA-3'
Viral hemorrhagic septicemia virus(VHSV) / Nucleoprotein (N) / VN forward / 5'-ATGGAAGGAGGAATTCGTGAAGCG-3' / [22] / Chapter 2.3.9
VN reverse / 5'-GCGGTGAAGTGCTGCAGTTCCC-3'
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