PC2 LABORATORY INSPECTION CHECKLIST
Inspection Team:Laboratory Room No.: / Laboratory Supervisor: / Inspection Date:
NB: Boxes to be ticked as items are sighted ()or notpresent (), or otherwise NA indicates the item is not applicable to this laboratory.
No. / Question / //NA / Corrective Actions / Responsible Person / Target Completion Date1 / Facilities
1.1 / Is there appropriate signage on entrances to the laboratory?
(a)Biohazard symbol
(b)Level of containment
(c)Access restrictions
1.2 / Are there stations for emergency:
(a)eyewashing
(b)safety showers
1.3 / Are ceilings, walls and floors smooth, easy to clean, impermeable to liquids, and resistant to commonly used reagents and disinfectants?
1.4 / Do floors have a non-slip finish?
1.5 / Have internal fittings and fixtures been fitted to minimize the collection of dust?
1.6 / Are there hands free basin mixers for handwashing near to laboratory exits?
1.7 / Is the water supplied to the laboratory fitted with backflow prevention?
1.8 / Is there a directional air flow maintained by extracting room air?
1.9 / Is there a pressure steam sterilizer for sterilizing infectious laboratory waste?
1.10 / Are there biohazard stickers posted on storage units which are kept outside the designated laboratory for microorganisms or recombinant or manipulated DNA ?
1.11 / Are there suitable coat hooks available for every individual’s laboratory gown? Are the hooks adjacent to the laboratory access door?
1.12 / Are protective covers kept on keyboards which are housed on work benches?
1.13 / Is there a supply of clearly labelled disinfectants for decontamination purposes?
1.14 / Are containers for infectious materials available?
2 / PPE
2.1 / Does protective clothing afford the protection to the front part of the body?
2.2 / Is footwear completely enclosed?
2.3 / Are gloves worn:
(a) when working in a biological safety cabinet?
(b) when handling human blood and bodily fluids?
2.4 / Are goggles or visors worn:
(a) to protect eyes from contaminated or dangerous materials, or from UV light?
(b) when subculturing organisms in a biological safety cabinet?
3 / Containment Equipment
3.1 / Is a biological safety cabinet used for manipulations where aerosols may be produced?
3.2 / Are biosafety cabinets serviced and certified annually?
3.3 / Are centrifuges fitted with either sealed rotors or safety cups where large volumes or high concentrations of infectious material is being used?
4 / Work Practices
4.1 / Is access to the laboratory limited to laboratory personnel and persons specified by laboratory management?
4.2 / Are doors kept closed while laboratory work is in progress?
4.3 / Have laboratory personnel received instruction and training, with regular updates, in handling pathogens?
4.4 / Are sharps being disposed ofin anapproved sharps containers?
4.5 / Are maintenance and service personnel advised of the special microbiological hazards in the laboratory? Are potentially contaminated surfaces disinfected before maintenance of equipment is conducted?
4.6 / Are containers of viable microorganisms transported between laboratories within a second unbreakable closed container which can be readily decontaminated?
4.7 / Is waste disposed of appropriately?
4.8 / Is special care taken when handling human blood, serum, other bodily fluids and substances?
4.9 / Are separate work areas within the laboratory provided for reference documents, papers and writing space?
4.10 / Has a pest control program against insects, birds and animals been instituted?
Other Comments :
Definitions
PC1/PC2:Physical Containment Level 1 or 2
PPE:Personal Protective Equipment
The University of Western Australia / PC2 Laboratory Inspection Checklist / Page 1 of 2Created: January 2009 / Last Review: /
Next Review:
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