MALDI Biotyper: the Next Generation Microbial Identification System for the 21St Century

MALDI Biotyper: The next generation microbial identification system for the 21st century The MALDI Biotyper enables an unbiased identification of microorganisms. It can be applied to gram-positive and gram-negative bacteria, yeast and multicellular fungi without any presumptions or pretesting. Starting from culture plates identification results can be generated in a couple of minutes. The MALDI Biotyper covers applications from clinical microbiology, food and feed safety and analysis, as well as industrial quality control.The MALDI Biotyper for identification of microorganisms is a system that meets all the demands defined for a revolutionary new approach - based on advanced, yet well acknowledged technology: mass spectrometry. Bruker offers the next generation for identifying microorganisms in your lab:

• Easy sample preparation
• Fast
• Robust
• Reliable mass spectrometric instrumentation
• Easy to use software (non MS-expert approved)

The easy & robust protocols for sample preparation starting e.g. from single bacterial colonies allow for a fast and cost – effective sample processing.
In a MALDI-TOF mass spectrometer, proteins and peptides are separated by their mass – revealing a characteristic peak pattern, the individual molecular fingerprint.
The easy-to-use software MALDI Biotyper reliably identifies microorganisms by the species-specific signal patterns contained in their respective molecular profiles in correlation to reference profiles. The whole process (data acquisition, analysis, identification, output) is performed by this single dedicated software.
A unique and continuously enlarged library containing thousands of reference entries enables secure identification of your current sample with respect to the references using sophisticated pattern matching algorithms.
It takes only minutes to receive the results. With only five mouse clicks the system is started and delivers a clear result. Options for a more detailed analysis such as sub-typing, principal component analysis, dendrogram generation or creation of own reference files are also included in the software.
The MALDI Biotyper is already successfully implemented in an increasing number of clinical routine labs. The MALDI Biotyper

• Automated next generation microbial identification system based on benchtop microflex MALDI-TOF mass spectrometer
• Unbiased identification of bacteria, yeasts and multicellular fungi
• From agar plate to identification in a few minutes
• Cost-effective sample preparation
• High resolution identification down to species level in one step
• Analysis of mixed cultures
• Interfaces for LIMS integration

-The MALDI Biotyper is a new method to reliably identify microorganisms using MALDI (matrix assisted laser desorption ionization) mass spectrometry, sophisticated protein peak pattern profiling algorithms and a dedicated database containing thousands of reference entries. In contrast to contemporary biochemical methods no initial data input is requested – every sample, including non-fermenters, is processed and analyzed the very same way. The system is very simple to use, robust, reliable, fast and cost effective.

-

-Improved method for fungal identification using MALDI-TOF mass spectrometry