Impact of a Low Energy Diet in the Fecal Microbiota of Obese Individuals

European Journal of Nutrition

Impact of a low energy diet in the fecal microbiota of obese individuals

Simões C.D.1*, Maukonen J.1, Scott K.P.2, Virtanen K.A.3, Pietiläinen K.H.4,5,6,7 & Saarela M.1

1 VTT Technical Research Centre of Finland; 2 Rowett Institute for Nutrition and Health, University of Aberdeen; 3 Turku PET Center, Turku University Central Hospital; 4 Obesity Research Unit, Department of Medicine, Division of Endocrinology, Helsinki University Central Hospital; 5 Research Programs Unit, Diabetes and Obesity Research Program, University of Helsinki; 6 FIMM Institute for Molecular Medicine Finland, University of Helsinki;7 Institute of Clinical Medicine, University of Helsinki

*Corresponding author: Catarina D. Simões;

Supplementary Table 1 Bacterial pure cultures used in this study for optimization of the methanogen group qPCR.

1 Number of the clostridial phylogenetic cluster [1]

2 National Center for Biotechnology Information, NCBI.

Supplementary Table 2 Primers used in the present study.

1 Partial 16S rRNA gene (V6-V8hypervariable region)

2 Partial 16S rRNA gene (V3-V5hypervariable region)

3 Clostridial phylogenetic cluster XIVa[1]

4 Clostridial phylogenetic cluster IV [1]

5Atopobium-group comprises of genera such asAtopobium, Eggerthella, and Collinsella

6Lactobacillus-group comprises of genera such asLactobacillus, Leuconostoc, Pediococcus, and Weissella

Supplementary Table 3 Nucleotide sequences of oligonucleotide probes used in this study.

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