HH PART II (Form Pages 7 to 9)

BIOGRAPHICAL SKETCH

1. Positions and Honors

Professional Experience:

1988-1989Lab Technician, Allied Signal, Des Plaines, Illinois

1991Lab Technician, Stepan Chemical Company, Northfield, Illinois.

1991-1993Lab Assistant, Pharmacology Department, Univ. of Health Sciences, North Chicago, Illinois

1993-1995Graduate Student, Biochemistry Department, University of Wisconsin, Madison, Wisconsin

1995-2000Research Intern Graduate Student, Department of Pathobiological Sciences, Animal Health Biomedical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI

2000-2002Post-Doctoral Research Fellow, Department of Surgery, Medical College of Wisconsin, Milwaukee, Wisconsin

2002-2004Research Associate, Department of Surgery, Feinberg School of Medicine, Northwestern University, Chicago, Illinois

2004–presentAssistant Research Professor, Department of Surgery, Feinberg School of Medicine,

Northwestern University, Chicago, Illinois

Honors and Awards:

2000AACR Young Investigator Scholar Award

2000AACR-AstraZeneca Scholar in Training Award

2001Post Doctoral Research Fellowship, Arnie and Viola Mienerz Foundation

2002Fellowship Award, Michael Rolfe Research Foundation

2004 Hirshberg Award: Best Abstract in Pancreatic Cancer 2004 Annual APA Meeting

2005 Hirshberg Award: Best Abstract in Pancreatic Cancer 2005 Annual APA Meeting

2007PanCAN-AACR Career Development Award

2009member of the NUACUC

Societies:

1999 – presentAmerican Association for Cancer Research (AACR), Member

2004 – presentAmerican Pancreatic Association (APA), Member

2007 – 2008American Gastroenterological Association (AGA), Member

Peer Review - Journals:

British Journal of Nutrition, Cancer Biology & Therapy,Cancer Investigation, Cancer Research, Cancer Prevention Research, Clinical Cancer Research, Gastroenterology, Genesis, Journal of the American Medical Association (JAMA), Metabolism, Molecular Cancer Research, Molecular Cell Research, Oncogene, Pancreas, and Pancreatology

Peer Review –Grants 2008-2009:

2008reviewer of a proposal for the Pfund Program – EPSCoR for the State of Lousiana

2009, 2010reviewer on the Chemoprevention & Dietary Prevention (CDP) study section – NCI/NIH

2009reviewer of Innovator Grants in Pancreatic Cancer – Cancer Council of New South Wales

2010reviewer of grant applications - Italian Ministry of Health

Edit Text/Author Book Chapters:

Pancreatic Cancer (AM Lowy, SD Leach, PA Philip)

Chapter 9: Models of Pancreatic Cancer: Understanding Disease Progression

LG Melstrom & PJ Grippo pp. 137-158 © 2008

Drug Discovery in Pancreatic Cancer: Models and Techniques (H Han & P Grippo)

Chapter 1: Drug Evaluations in Pancreatic Cancer Culture Systems

B. Mullapudi, YZ Ding, XZ Ding, PJ Grippo pp. 1-28 © 2010

1. Selected Peer Reviewed Publications

1. Hentosh P, McCastlain JC, Grippo P, Bugg BY. Polymerase chain reaction amplification of single-stranded DNA containing a base analog, 2-chloradenine. Anal Biochem. 1992 201(2):277-81.
2. Hentosh P, Grippo P. Template 2-chloro-2'-deoxyadenosine monophosphate inhibits in vitro DNA synthesis. Mol Pharmacol. 1994 45(5):955-61.
3. Hentosh P, Grippo P. 2-Chloro-2'-deoxyadenosine monophosphate residues in DNA enhance susceptibility to 3'-->5' exonucleases. Biochem J. 1994 302 ( Pt 2):567-71.
4. Hentosh P, Tibudan M, Grippo P. A human factor that recognizes DNA substituted with 2-chloroadenine, an antileukemic purine analog. Mol Carcinog. 1995 13(4):245-53.
5. Grippo PJ, Sandgren EP. Highly invasive transitional cell carcinoma of the bladder in a simian virus 40 T-antigen transgenic mouse model. Am J Pathol. 2000 157(3):805-13.
6. Yen TW, Sandgren EP, Liggitt HD, Palmiter RD, Zhou W, Hinds TR, Grippo PJ, McDonald JM, Robinson LM, Bell RH Jr. The gastrin receptor promotes pancreatic growth in transgenic mice. Pancreas. 2002 24(2):121-9.
7. Grippo PJ, Nowlin PS, Cassaday RD, Sandgren EP. Cell-specific transgene expression from a widely transcribed promoter using Cre/lox in mice. Genesis. 2002 32(4):277-86.
8. Brembeck FH, Schreiber FS, Deramaudt TB, Craig L, Rhoades B, Swain G, Grippo P, Stoffers DA, Silberg DG, Rustgi AK. The mutant K-ras oncogene causes pancreatic periductal lymphocytic infiltration and gastric mucous neck cell hyperplasia in transgenic mice. Cancer Res. 2003 63(9):2005-9.
9. Grippo PJ, Nowlin PS, Demeure MJ, Longnecker DS, Sandgren EP. Preinvasive pancreatic neoplasia of ductal phenotype induced by acinar cell targeting of mutant Kras in transgenic mice. Cancer Res. 200363(9):2016-9.
10. Grippo PJ, Sandgren EP. Modeling pancreatic cancer in animals to address specific hypotheses. Methods Mol Med. 2005;103:217-43. Review.
11. Hennig R, Grippo P, Ding XZ, Rao SM, Buchler MW, Friess H, Talamonti MS, Bell RH, Adrian TE. 5-Lipoxygenase, a marker for early pancreatic intraepithelial neoplastic lesions. Cancer Res. 200565(14):6011-6.
12. Guerra C, Schuhmacher AJ, Canamero M, Grippo PJ, Verdaguer L, Perez-Gallego L, Dubus P, Sandgren EP, Barbacid M. Chronic pancreatitis is essential for induction of pancreatic ductal adenocarcinoma by K-Ras oncogenes in adult mice. Cancer Cell. 2007 11(3):291-302.
13. Salabat MR, Melstrom LG, Strouch MJ, Ding XZ, Milam BM, Ujiki MB, Chen C, Pelling JC, Rao S, Grippo PJ, McGarry TJ, Bentrem DJ Geminin is overexpressed in human pancreatic cancer and downregulated by the bioflavanoid apigenin in pancreatic cancer cell lines. Mol Carcinog. 2008 Apr 10.
14. Warner S, Munoz R, Bearss D, Grippo P, Han H, VonHoff D. Pdx-1-Driven Overexpression of Aurora A Kinase Induces Mild Dysplasia of Pancreatic Ducts near Islets in Transgenic Mice. Pancreas. 200837(3):e39-44.
15. Strouch MJ, Ding Y, Salabat MR,Melstrom LG, Adrian K, Quinn C, Pelham C, Rao S, Adrian TE, Bentrem DJ, Grippo PJ A High Omega-3 Fatty Acid Diet Mitigates Murine Pancreatic Precancer Development.

J of Surg Res2011 Jan;165(1):75-81.

16. Adrian K, Strouch MJ, Zeng Q, Cheon E, Honasoge A, Barron MR, Xu Y, Phukan S, Sadim M, Bentrem DJ, Pasche B, Grippo PJ. Tgfbr1 Haploinsufficiency Inhibits the Development of Murine Mutant Kras-Induced Pancreatic Precancer. Cancer Research2009; 69(24):9169-74.

17. Ding Y, Cravero JD, Adrian K, Grippo P. Modeling pancreatic cancer in vivo: from xenograft and carcinogen-induced systems to genetically engineered mice. Pancreas. 2010 Apr;39(3):283-92. Review.

18. Cheon EC, Strouch MJ, Barron MR, Ding YZ,Melstrom LG, Krantz SB, Mullapudi B, Adrian K, Rao S, Adrian TE, Bentrem DJ, Grippo PJ. Alteration of strain background and a high omega-6 fat diet induces earlier onset of pancreatic neoplasia in EL-Kras transgenic mice. International Journal of Cancer (in press).

19. Grippo PJ, Tuveson DA. Deploying mouse models of pancreatic cancer for chemoprevention studies

Cancer Prev Res (Phila). 2010 Nov;3(11):1382-7.

20. Dangi-Garimella, S, Strouch MJ, Grippo, PJ, Bentrem DJ, Munshi HG. Collagen regulation of let-7 in pancreatic cancer involves TGF-β1-mediated membrane type 1-matrix metalloproteinase expression. Oncogene (in press).

21. Dangi-Garimella S, Krantz SB, Barron MR, Shields MA, Heiferman M, Grippo PJ, Bentrem DJ, Munshi HG. Three dimensional collagen I promotes gemcitabine resistance in pancreatic cancer through MT1-MMP-mediated expression of HMGA2. Cancer Research (in press).

22. Cheon E, Khazaie K, Khan MW, Strouch MJ, Krantz SB, Phillips J, Blatner NR, Hix LM, Zhang M, Dennis KL, Salabat MR, Heiferman M, Grippo PJ, Munshi HG, Gounaris E, Bentrem DJ. Mast cell 5-Lipoxygenase activity promotes intestinal polyposis in APC{Delta}468 mice.Cancer Research (in press).

23. Melstrom LG, Salabat MR, Ding XZ, Strouch MJ, Grippo PJ, Mirzoeva S, Pelling JC, Bentrem DJ.

Apigenin Down-Regulates the Hypoxia Response Genes: HIF-1α, GLUT-1, and VEGF in Human Pancreatic Cancer Cells.J Surg Res. (in press).

1. Completed Research Support

Zell Scholarship Award09/01/07 – 08/31/10

Michael Zell and the RobertH.LurieCancerCenter (Grippo)

Development and Use of Mouse Models of Pancreatic Cancer

The primary purpose of this project is to expand the current pool of mouse models of pancreatic cancer and explore their use for evaluating systems of early detection, chemoprevention, and therapy.

1 R21 CA123041-01A1 (Grippo)06/20/07 – 06/19/09

National Institutes of Health (NCI)

Omega-6 Fat Promotion of Mutant Kras-Induced Pancreatic Neoplasia

The main theme of this project is to demonstrate the role of 5-Lox and Cox-2 as the primary mechanism for the promotion of mutant Kras-induced pancreatic precancerous lesions by omega-6 fats.

07-20-25-GRIP -Career Development Award (Grippo)07/01/07 – 06/30/09

Pancreatic Cancer Action Network-American Association for Cancer Research

Evaluating Kras Oncogene Addiction in Pancreatic Precancer and Cancer

This proposal will investigate the dependence of mutant Kras-induced precancerous lesions and cancer of the pancreas on the maintenance of mutant Kras expression.

Research Grant (Grippo)04/01/07 – 03/31/08

American Medical Association

Determining the Anti-Proliferative Mechanism of Omega-3 Fatty Acids on Pancreatic Cancer Cells in Vitro and in a Transgenic Mouse Model in Vivo

Alumnae of Northwestern University (Grippo)06/01/07 – 06/25/07

Research Grant

These funds were used to purchase equipment for establishing and maintaining a mouse database.

Career Development Award (Bentrem)07/01/06 – 06/30/08

American Society of Clinical Oncology (Role: Co-Investigator)

This project identifies the effectsofcombined therapywithapigeninandgemcitabineoncellproliferationin human pancreatic cancer cells and murine tumor models.

LF04-023 (Grippo)01/07/04 – 09/30/05

Lustgarten Foundation for Pancreatic Cancer Research

Dual Targeting of mutant Kras and Mucin1 in Mice that Develop Human-like PanINs.

This project will determine if Mucin1 will play a role in Kras-induced pancreatic carcinogenesis, serve as an ideal antigen for targeting, and, in combination with mutant Kras, be a superior means of targeting pancreatic cancer cells.