Online Resource 3. Intra-population summary statistics based on 18 Agrostis stolonifera (L.) chloroplast microsatellite (cpSSR) markers used to genotype 78 Agrostis, Apera, and Polypogon cultivars, experimental selections, and accessions. Summary statistics generated in HAPLOTYPE ANALYSIS 1.05 (Eliades and Eliades 2009).

Number Number Mean

of of Effective Genetic

haplotypes private number Diversity

Cultivar, Sample size detected haplotypes of Haplo- between

selection, in each in each in each haplo- typic Genetic indi-

or accession population population population types richness Diversity viduals

(Population)a (N)b (A)b (Ph)bc (Ne)bd (Rh)be (He)bf (D2sh)bg

OO7 / 16 / 9 / 3 / 5.82 / 8.00 / 0.88 / 2.56
13M / 16 / 8 / 2 / 5.57 / 7.00 / 0.88 / 3.00
PI255869 (A. gigantea) / 16 / 6 / 6 / 2.37 / 5.00 / 0.62 / 53.71
PI619558 (A. gigantea) / 16 / 6 / 5 / 2.37 / 5.00 / 0.62 / 20.71
AP18 / 16 / 4 / 0 / 3.12 / 3.00 / 0.73 / 0.06
PI283174 (A. transcaspica) / 16 / 3 / 3 / 1.86 / 2.00 / 0.49 / 0.06
PI221906 Afghanistan / 16 / 4 / 2 / 1.71 / 3.00 / 0.44 / 1.11
Alister (A. capillaris) / 16 / 4 / 3 / 1.94 / 3.00 / 0.52 / 0.24
Alpha / 16 / 4 / 2 / 2.25 / 3.00 / 0.59 / 1.78
Authority / 16 / 7 / 2 / 2.84 / 6.00 / 0.69 / 1.42
Barracuda / 16 / 9 / 4 / 5.82 / 8.00 / 0.88 / 2.19
Benchmark DSR / 16 / 7 / 1 / 5.33 / 6.00 / 0.87 / 2.50
PI608809 US / 16 / 1 / 0 / 1.00 / 0.00 / 0.00 / 0.00
CDE Comp. (A. canina) / 16 / 8 / 3 / 5.12 / 7.00 / 0.86 / 4.40
Capri (A. capillaris) / 16 / 4 / 1 / 2.72 / 3.00 / 0.68 / 0.09
Carmen / 16 / 4 / 0 / 1.94 / 3.00 / 0.52 / 0.21
Century / 16 / 4 / 0 / 1.71 / 3.00 / 0.44 / 0.99
Crenshaw / 16 / 4 / 2 / 2.51 / 3.00 / 0.64 / 0.80
Declaration / 16 / 5 / 4 / 2.00 / 4.00 / 0.53 / 0.51
EBM (A. capillaris) / 16 / 6 / 0 / 4.92 / 5.00 / 0.85 / 0.24
Emerald / 16 / 4 / 2 / 1.71 / 3.00 / 0.44 / 0.27
FT12 (A. capillaris) / 16 / 2 / 0 / 1.28 / 1.00 / 0.23 / 0.01
Germany PI259838 / 16 / 2 / 2 / 1.13 / 1.00 / 0.13 / 0.08
Unknown Creep. Bentgrass / 16 / 8 / 7 / 5.57 / 7.00 / 0.88 / 2.16
PI531251 Hungary / 16 / 4 / 3 / 2.72 / 3.00 / 0.68 / 0.17
Imperial / 16 / 9 / 4 / 4.27 / 8.00 / 0.82 / 2.06
Independence / 16 / 8 / 2 / 5.57 / 7.00 / 0.88 / 1.29
Kingpin / 16 / 7 / 1 / 3.28 / 6.00 / 0.74 / 1.11
PI494118 Ukraine / 16 / 8 / 6 / 5.12 / 7.00 / 0.86 / 0.63
PI659907 Kyrgyzstan / 16 / 7 / 4 / 3.66 / 6.00 / 0.78 / 1.48
L93 / 16 / 9 / 4 / 5.12 / 8.00 / 0.86 / 2.11
Legendary (A. canina) / 16 / 6 / 6 / 2.72 / 5.00 / 0.68 / 8.38
Memorial / 16 / 6 / 2 / 2.78 / 5.00 / 0.68 / 1.40
National / 16 / 1 / 1 / 1.00 / 0.00 / 0.00 / 0.00
PI222073 Afghanistan / 16 / 4 / 2 / 2.17 / 3.00 / 0.58 / 0.05
PI230235 Iran / 16 / 3 / 3 / 2.17 / 2.00 / 0.58 / 0.25
PI608810 US / 16 / 2 / 1 / 1.13 / 1.00 / 0.13 / 0.26
PI608811 US / 16 / 4 / 3 / 1.71 / 3.00 / 0.44 / 0.13
PennA4 / 16 / 3 / 0 / 1.29 / 2.00 / 0.24 / 0.05
Penncross / 16 / 10 / 3 / 6.40 / 9.00 / 0.90 / 2.60
Penneagle II / 16 / 9 / 1 / 6.10 / 8.00 / 0.89 / 2.04
Penneagle / 16 / 11 / 4 / 8.00 / 10.00 / 0.93 / 1.43
Pennlinks / 16 / 5 / 2 / 2.91 / 4.00 / 0.70 / 0.11
Pinup / 16 / 6 / 2 / 3.66 / 5.00 / 0.78 / 2.82
Proclamation / 16 / 6 / 1 / 4.00 / 5.00 / 0.80 / 0.93
Providence / 16 / 12 / 9 / 8.53 / 11.00 / 0.94 / 3.03
Putter / 16 / 4 / 3 / 1.97 / 3.00 / 0.53 / 0.08
SME Comp. (A. canina) / 16 / 6 / 2 / 4.27 / 5.00 / 0.82 / 3.84
SMM Comp. (A. canina) / 16 / 3 / 0 / 1.47 / 2.00 / 0.34 / 1.54
SR1119 / 16 / 4 / 0 / 2.51 / 3.00 / 0.64 / 1.79
SR7100 (A. capillaris) / 16 / 10 / 6 / 8.00 / 9.00 / 0.93 / 1.13
SR7150 (A. capillaris) / 16 / 4 / 0 / 3.37 / 3.00 / 0.75 / 0.06
SR7200 (A. canina) / 16 / 5 / 3 / 3.12 / 4.00 / 0.73 / 4.11
Focus / 16 / 9 / 5 / 5.33 / 8.00 / 0.87 / 0.96
SRP1WM / 16 / 7 / 2 / 4.74 / 6.00 / 0.84 / 2.10
Seaside / 16 / 7 / 3 / 3.88 / 6.00 / 0.79 / 1.03
Shark / 16 / 9 / 1 / 5.12 / 8.00 / 0.86 / 2.29
Southshore / 16 / 7 / 1 / 5.12 / 6.00 / 0.86 / 2.19
PI439027 SovietUnion / 16 / 12 / 11 / 8.53 / 11.00 / 0.94 / 55.12
PI302902 Spain / 16 / 8 / 7 / 3.46 / 7.00 / 0.76 / 24.46
PI318934 Spain / 16 / 13 / 10 / 10.67 / 12.00 / 0.97 / 54.19
PI423036 Spain / 16 / 3 / 3 / 1.29 / 2.00 / 0.24 / 0.09
PI235541 Sweden / 16 / 8 / 7 / 5.12 / 7.00 / 0.86 / 0.71
PI235440 Switzerland / 16 / 2 / 1 / 1.44 / 1.00 / 0.33 / 0.01
T1 / 16 / 7 / 1 / 4.92 / 6.00 / 0.85 / 3.34
Luminary / 16 / 6 / 1 / 4.00 / 5.00 / 0.80 / 1.23
Tiger II (A. capillaris) / 16 / 2 / 0 / 2.00 / 1.00 / 0.53 / 0.02
PI204390 Turkey / 16 / 2 / 1 / 1.13 / 1.00 / 0.13 / 0.02
PI608812 US / 16 / 4 / 3 / 2.56 / 3.00 / 0.65 / 0.63
PI608813 US / 16 / 10 / 8 / 8.00 / 9.00 / 0.93 / 8.99
W621826 Ukraine / 16 / 14 / 13 / 12.80 / 13.00 / 0.98 / 16.42
V8 / 16 / 8 / 3 / 3.46 / 7.00 / 0.76 / 2.46
VTP Comp. (A. canina) / 16 / 8 / 6 / 6.74 / 7.00 / 0.91 / 2.96
Villa (A. canina) / 16 / 9 / 6 / 6.10 / 8.00 / 0.89 / 1.97
PI203444 (Apera interrupta) / 16 / 3 / 2 / 2.17 / 2.00 / 0.58 / 0.08
PI204389 (Apera interrupta) / 16 / 1 / 0 / 1.00 / 0.00 / 0.00 / 0.00
PI372227 (Poly. chilensis) / 16 / 1 / 1 / 1.00 / 0.00 / 0.00 / 0.00
PI204395 (Poly. viridis) / 16 / 1 / 1 / 1.00 / 0.00 / 0.00 / 0.00
Mean / 16 / 5.90 / 2.94 / 3.76 / 4.90 / 0.65 / 4.17

a Cultivar, experimental selection, or accession is Agrostis stolonifera, unless otherwise noted in italics.

b Abbreviations from HAPLOTYPE ANALYSIS 1.05 (Eliades and Eliades 2009).

c Private haplotypes = haplotypes unique to any given population.

d Effective number of haplotypes (Ne), where Ne = 1/(Σpi2).

e Haplotypic richness (Rh), where Rh = Σ[1-(n-p/n)]; Rh estimated based on the rarefaction method (El Mousadik and Petit 1996).

f Nei’s unbiased genetic diversity (He), where He = ((n/(n − 1))(1 − Σpi2)); from Nei (1973).

g Mean genetic distance between individuals (D2sh); from Goldstein et al. (1995).

References

El Mousadik A, Petit RJ (1996) High level of genetic differentiation for allelic richness among populations of the argan tree [Argania spinosa (L.) Skeels] endemic to Morocco. Theor Appl Genet 92:832-839

Eliades NG, Eliades DG (2009) HAPLOTYPE ANALYSIS: software for the analysis of haplotype data. Distributed by the authors. Forest genetics and forest tree breeding, Georg-Augst University Goettingen, Germany

Goldstein DB, Linares AR, Cavallisforza LL, Feldman MW (1995) An evaluation of genetic distances for use with microsatellite loci. Genetics 139:463-471

Nei M (1973) Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci USA 70:3321-3323

Classification of bentgrass (Agrostis) cultivars and accessions based on microsatellite (SSR) markers. Genetic Resources and Crop Evolution. Josh A. Honig*, Christine Kubik, Vincenzo Averello, Jennifer Vaiciunas, William A. Meyer, and Stacy A. Bonos. *Dep. of Plant Biology and Pathology, School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, 59 Dudley Rd., New Brunswick, NJ 08901-8520,