Table S2: Mean recoveries and relative standard deviations (% RSD) for control samples under UHPLC-MS/MS (0.6 mL min-1) investigation.
Compound / Precursor ion (m/z) / Spiked Conc.(µg kg-1)* / Mean Recovery
(%, n=5) / Intraday
(% RSD, n=5) / Mean Recovery (%, n=14) / Interday
(% RSD, n=14)
Crotaline / 326.2 / 35.7 / 99 / 2 / 98 / 4
71.4 / 89 / 27 / 77 / 29
Crotaline-N-oxide / 342.2 / 35.7 / 125 / 4 / 120 / 5
71.4 / 87 / 25 / 76 / 27
Lycopsamine / 300.2 / 35.7 / 83 / 4 / 84 / 4
71.4 / 85 / 2 / 84 / 3
Retrorsine / 352.2 / 35.7 / 89 / 3 / 93 / 10
71.4 / 91 / 5 / 94 / 6
Heliotrine / 314.2 / 35.7 / 108 / 5 / 108 / 6
71.4 / 106 / 2 / 106 / 4
Retrorsine-N-oxide / 368.2 / 35.7 / 94 / 3 / 98 / 6
71.4 / 93 / 3 / 95 / 5
Trichodesmine / 354.2 / 35.7 / 73 / 1 / 71 / 7
71.4 / 71 / 3 / 71 / 3
Otosenine / 382.2 / 35.7 / 115 / 4 / 117 / 7
71.4 / 112 / 2 / 112 / 6
Seneciphylline / 334.2 / 35.7 / 86 / 8 / 89 / 7
71.4 / 86 / 3 / 87 / 4
Seneciphylline-N-oxide / 350.2 / 35.7 / 76 / 3 / 77 / 4
71.4 / 77 / 2 / 77 / 3
Senecionine / 336.2 / 35.7 / 87 / 7 / 90 / 6
71.4 / 87 / 3 / 89 / 4
Senecionine-N-oxide / 352.2 / 35.7 / 79 / 1 / 80 / 3
71.4 / 79 / 1 / 80 / 2
Echimidine / 398.2 / 35.7 / 127 / 4 / 129 / 7
71.4 / 125 / 1 / 126 / 5
Senkirkine / 366.2 / 35.7 / 94 / 5 / 95 / 4
71.4 / 94 / 3 / 94 / 3
* PA concentration spiked into blank honey extract
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