MA Khaleque/CV/Feb_2016
Md Abdul Khaleque, PhDDirector, Office of Admissions
Professor, Department of Biology & Chemistry
North South University, Dhaka 1229 /
Home address: House- 7, Road- 9, Sector- 4
Uttara, Dhaka- 1230
Phone: 02-8961048 (res)
0171-4178-729 (cell)
Email:
Work address: Department of Biology & Chemistry
North South University
Plot# 15, Block# B
Bashundhara R/A, Dhaka 1229
Tel: 880-2-55668200 Ext. 1180
Email:
Education
Ph.D in Molecular Genetics, Kumamoto University, Japan 2002 Degree Awarded
M.S. in Biochemistry and Molecular Biology, University of Dhaka 1994 (First Class)
B.S. in Biochemistry and Molecular Biology, University of Dhaka 1992 (First Class)
HSC from Carmichael College, Rangpur (Rajshahi Board) 1987 (First Division)
SSC from Rangpur Zilla School, Rangpur ((Rajshahi Board)) 1985 (First Division)
Professional appointments
05/2015- present Director, Office of Admissions and Professor, Dept. of Biology & Chemistry North South University
05/2014- 5/2015 Associate Professor & Chairman, Dept. of Biology & Chemistry, North South University, Dhaka.
01/2013- 04/2014 Associate Professor, Dept. of Biochemistry & Microbiology, North South University, Dhaka.
12/2011- 12/2012 Associate Professor & Chairman, Dept. of Biochemistry & Microbiology, North South University, Dhaka.
05/2009- 12/2011 Assistant Professor & Chairman, Dept. of Biochemistry & Microbiology, North South University, Dhaka.
08/2007- 04/2009 Assistant Professor, Dept. of Biochemistry & Microbiology, North South University, Dhaka
Teaching Genes and Diseases, Basic Biochemistry and Biotechnology, Biology I, Molecular Biology, Metabolism, Seminar, Laboratory courses and Biochemistry for MS Biotechnology, Proteins and Enzymes
07/2006-07/2007 Scientist II, Zymed Division, Invitrogen Corporation, California, USA
Worked under Commercial Operation Department for the development of Cellular and Molecular Biology products such as pathology and research antibody, reagents etc. Conducted several lectures and training programs on cellular and molecular biology product development.
12/2003-07/2006 Post-Doctoral Research Associate, Harvard Medical School, Boston, Massachusetts, USA
Worked in the Department of Radiation Oncology and completed several research projects on the role of heat shock proteins in cancer and published five full articles in journals like Treads in Biochemical Science, JBC, Oncogene, Journal of Immunology etc. Supervise the Research work & Thesis of several Masters Students.
Teaching: Basic Biochemistry and Molecular Biology
11/2002-11/2003 Post-Doctoral Research Associate, Boston University, Boston, Massachusetts, USA
Worked in the Department of Medicine (Stress Response Unit) and successfully completed the project “Expression of heat shock proteins and HSP mRNA in human prostate carcinoma in vitro and in tumors” which was published in JBC and Cell Stress and Chaperones.
Supervise the Research work & Thesis of several Students.
Teaching: Molecular Genetics, Biochemistry etc.
02/1996-09/1997 Scientific Officer, National Diagnostic Network, Dhaka
Department of Biochemistry and Endocrinology
10/1995-01/1996 Research Officer, ICDDRB, Dhaka
Department of Nutrition under Laboratory Science Division.
06/1994-09/1995 Research Fellow, BIRDEM, Dhaka
Department of Pharmacology and Molecular Biology under Research Division
Technical Expertise
Molecular Biology: All general molecular biology techniques including but not limited to:
Genomic DNA isolation from tissue and cultured cells, plasmid DNA preparation, cDNA library construction, cDNA and genomic DNA library screening and clone isolation, DNA sequencing, RNA isolation from whole tissue and cultured cells, PCR for various application including site directed mutagenesis and RT-PCR, silencing of genes using RNAi, northern blots and western blots, chromatin immunoprecipitation (ChIP) assay.
Molecular Pathology: CISH (Chromogenic In-situ Hybridization), FISH (Fluorescence In-situ Hybridization), IHC (Immunohistochemistry), IF (Immunofluorescence).
Biochemistry: Protein expression and purification, SDS-PAGE, Blue native PAGE, ELISA, protein import into mitochondria, techniques for protein phosphorylation and signal transduction mechanism.
Tissue culture/ cell based assay: Mammalian cell culture (HeLa, MCF-7, CHO-K1, MDA-MB-231, MEF, H9c2 and others), transient and stable transfection, gene expression and characterization, anchorage independent growth assay.
Other skills: Luciferase assay, apoptosis assay, broad experience in signal transduction steps, tumor progression and metastasis.
Non-technical Expertise
Strong laboratory management skills, ability to train and supervise graduate/under graduate students, staff and junior lab personnel, ability to work independently, experience on grant writing and manuscript preparation, experience on planning, development and implementation of laboratory requirements, experience on purchasing laboratory reagent and instrument and above all excellent written and oral communication skills.
Industry experience
· Establish and maintain Quality Systems consistent with GLP, GMP, FDA regulation and ISO requirements. Assures compliance to in-house and external specifications and standards.
· Actively contribute to the data collection, trend analysis, investigation and improvements to processes, products and systems. Manages the Corrective and Preventative Action (CAPA) system.
· Work with QA personnel to ensure that appropriate scientific and regulatory improvements are properly introduced and phased into operations.
· Design, evaluate, implement and maintain QA programs for product and process development, manufacturing and testing to ensure product quality and compliance with regulatory requirements.
· Work with quality and operations to create and modify methods and procedures to ensure that appropriate processes are developed, tests are conducted.
· Work with the Client and Program Managers to identify all study/regulatory requirements and effectively communicate these requirements to the Operational and Study Directors.
· Responsible for providing pre- and post-sales technical support of Invitrogen cellular and molecular biology products to customers globally as well as relaying customer feedback to organization.
· Interface with R&D to monitor new assay development and introduction. Participate in direction of R&D work, comment on new guidance documents.
· Preparation of new and revised technical bulletins, expert reports, promotional brochures etc.
· Lead and provide work direction to junior personnel.
Publications
1. Ashish Kumar Majumder, Md. Abdul Khaleque, Kazi Nadim Hasan, Lamyah Sultana Meem, and Sharif Akhteruzzaman. Two cases of Klinefelter Syndrome Identified by Quantitative Fluorescence PCR (QF-PCR). Biores Comm. 1(1), 17-21 (2015)
2. Sohidul Islam, Obaidur Rahman, Mahmud Hossain and Abdul Khaleque. Antioxidant Activity of Some Common Seasonal Fruits of Bangladesh. Biores Comm. 1(1), 28-31 (2015)
3. Md. M. A. Khan, Narayan C. Paul, Md. A. Khaleque, Aniruddha Sarker, Md. T. Islam. Isolation and characterization of multifunctional phosphate solubilizing bacteria associated with rice (Oryza sativa L.) cultivated in Bangladesh. Submitted in ZNC (Zeitschrift für Naturforshung C- Journal of Bioscience). (2014)
4. M Abdul Khaleque, Faisal Bin Rashed, Fannana Rafa, Gias Uddin Ahsan, Donald James Gomes. miRNA: An Overview of In Silico Prediction Method. Editorial. Bang J Med Sci. 18(2), 80-83 (2012)
5. Kazi Nadim Hasan, Fahmida Nasrin, A Abu Taher, Sharif Neaz, Sohidul Islam, Obaidur Rahman, Ishrat Jabeen, M Abdul Khaleque, Sharif Akhteruzzaman. Comparative Analysis of Hepatitis C Virus (HCV) RNA, Anti-HCV and Liver Transaminase Levels as Markers and Predictors of Infectivity in HCV Infection. Bang J Med Sci. 18(2), 120-125 (2012)
6. Sohidul Islam, Lamyah Sultana Meem, Kazi Nadim Hasan, M Abdul Khaleque, Obaidur Rahman. Multidrug Resistance of Pseudomonus aeruginosa Isolated from Burn Patients. Bang J Med Sci. 18(2), 132-134 (2012)
7. Obaidur Rahman, Kazi Nadim Hasan, Abdul Khaleque, Ishrat Jabeen, Sohidul Islam. Dietary Intake of Urea from Puffed Rice (Muri) in Bangladesh. Bang J Med Sci. 18(1), 58-61 (2012)
8. Ishtiaq Mahmud, Mousomi Akter, Nuruzzaman Masum, M A Khaleque, Zillur Rahman, Rowshan Ara Islam, M Sohrab Alam, Emran Kabir Chowdhury, Shahdat Hossain. Serum Homocysteine and its Association with Cardiovascular Disease (CVD). J Bang Soc Pharm Professionals. 1(II), 11-26 (2012)
9. Md Abdul Khaleque, Ajit Bharti and Stuart K. Calderwood. Heregulin Induces Heal Shock Proteins in an HSF1 dependent Manner in cancer Cells and Leads to Protection from Apoptosis. In vivo. 25(3), 492-493 (2011)
10. S. K. Calderwood, Y. Xie, X. Wang, M. A. Khaleque, S. D Chou, A. Murshid, T. Prince and Y. Zhang. Signal Transduction Pathways Leading to Heat Shock Transcription. Signal Transduction Insights 2010:3 13-24 (2010)
11. Ishtiaq Mahmud, Dilshat Sultana, Nuruzzaman Masum, Hussain M Shahjalal, Shamima Keka Islam, M A Khaleque, SAM Khairul Bashar. Lipid Profile in Thyroid Disorders and the Risk of Atherosclerotic Cardiovascular Diseases in the Middle-Aged Population of Bangladesh. Bang J Med Sci. 15(2), 87-92 (2009)
12. Muhammad Maqsud Hossain, Fariza Shams, SAM Khairul Bashar, Md Abdul Khaleque, Nazmul Ahsan Khan, Kharirul Azam, Choudhury Rafiqul Ahsan, Donald James Gomes. Clinical aspects of Helicobacter pylori infection. Bang Med J. 14(2), 155-168 (2008).
13. Md Abdul Khaleque, Ajit Bharti, Jianlin Gong, Philip J. Gray, Daniel R. Ciocca, Arturo Stati, Mariel Fanelli and Stuart K. Calderwood. Heat Shock Transcription Factor 1 Represses Transcription Through Association with Metastasis Associated Protein 1. Oncogene, 27(13):1886-93 (2008).
14. Yutaka Enomoto, Ajit Bharti, Md Abdul Khaleque, Baizheng Song, Chunlei Liu, Vasso Apostolopoulos, Pei-xiang Xing, Stuart Calderwood and Jianlin Gong. Enhanced immunogenicity of heat shock protein 70 peptide complexes from dendritic cell-tumor fusion cells. J Immunol. 177: 5946-5955. (2006).
15. Stuart K Calderwood, Md Abdul Khaleque, Douglas B. Sawyer and Daniel R. Ciocca. Heat shock proteins in cancer: chaperones of tumoregenesis. Review. Trends Biochem Sci.31(3):164-72. (2006)
16. Md Abdul Khaleque, XiaoZhe Wang, Mei Juan Zhau, Rong Zhong, Matthias Gaestel and Stuart K Calderwood. Phosphorylation of HSF1 by MAPKAP Kinase 2 on serine 121, inhibits transcriptional activity and promotes HSP90 binding. J Biol Chem. 281(2):782-91. (2006). Equal contribution
17. Md Abdul Khaleque, Ajit Bharti, Douglas Sawyer, Jianlin Gong, Ivor J. Benjamin, Mary Ann Stevenson and Stuart K. Calderwood. Induction of heat shock proteins by heregulin b1 leads to protection from apoptosis and anchorage-independent growth. Oncogene, 24, 6564-6573 (2005).
18. Dan Tang, Md Abdul Khaleque, Ellen L. Jones, Jimmy R. Theriault, Cheng Li, Wing Hung Wong, Mary Ann Stevenson and Stuart K. Calderwood. Expression of heat shock proteins and HSP mRNA in human prostate carcinoma in vitro and in tumors. Cell Stress & Chaperones 10 (1), 46-58 (2005).
19. Donghui Yu, Ehsan Khan, Md Abdul Khaleque, James Lee, Gary Laco, Glenda Kohlhagen, Surender Kharbanda, Yung-Chi Cheng, Yves Pommier and Ajit Bharti. Phosphorylation of DNA Topoisomerase I by the c-Abl tyrosine kinase confers camptothecin sensitivity. J Biol Chem. 279(50):51851-61 (2004).
20. Khaleque Md Abdul, Kazutoyo Terada, Tomomi Gotoh, Rahman Md. Hafizur, Masataka Mori. Characterization and functional analysis of a heart-enriched DnaJ/Hsp40 homolog dj4/DjA4. Cell Stress & Chaperones 7(2), 156-166 (2002).
21. K Akhter, F Noor, MA Khaleque, N Rahman, S Shammy, T Mannan, N Kamal, JMA Hannan, L Ali. Acute effects of a low-calorie icecream on glycemic status and atherogenic risk factors of type 2 diabetic patients. Diabetes and Endocrine Journal 30(1), 14-16 (2002).
22. Khaleque Md Abdul, Kazutoyo Terada, Masato Yano, Michael T. Ryan, Illo Streimann, Nicholas J. Hoogenraad, Masataka Mori. Functional analysis of human metaxin in mitochondrial protein import in cultured cells and its relationship with the Tom complex. Biochem Biophys Res Commun 276, 1028-1034 (2000).
Dissertations
1. Md Abdul Khaleque, Kazutoyo Terada, Masataka Mori. Studies on a putative mitochondrial import factor metaxin and a new Hsp70 cochaperone dj4. Doctor’s Thesis submitted to Department of Molecular Genetics, Graduate School of Medicine Kumamoto University, Kumamoto, Japan (2002) [http://reposit.lib.kumamoto-u.ac.jp/handle/2298/209]
2. Md Abdul Khaleque, Liaquat Ali, Ishtiaq Mahmud. Changes in lipid profile and platelet aggregation in smokers of different duration. M.Sc Thesis submitted to Department of Biochemistry, University of Dhaka, Dhaka, Bangladesh (1995)
Book Chapter
1. HSF1: An Emerging Factor in Cancer. Stuart Calderwood, Md Abdul Khaleque and Daniel R. Ciocca. In: Heat Shock Proteins in Cancer. Ed: Stuart K. Calderwood. Publisher: Springer (2007)
2. Facts on Foodborne Pathogens. Abdul Khaleque and Md. Latiful Bari. In Foodborne Pathogens and Food Safety. Ed. Md Latiful Bari & Dike O. Ukuku. Publisher: CRC Press (Chapter 3, 2015)
3. Impact of Changing Lifestyles and Consumer Demands on Food Safety. Abdul Khaleque, Mohammad Mustafizur Rahman, Kazi Selim Anwar and Md. Nazim Uddin. In Foodborne Pathogens and Food Safety. Ed. Md Latiful Bari & Dike O. Ukuku. Publisher: CRC Press (Chapter 10, 2015)
Environmental article
EPHD: Commited to Raising the Quality of Life in Bangladesh. Md Abdul Khaleque, Md Sohrab Alam, Md Iftequar Wadud and Harun K.M. Yusuf. News Letter Kumamoto, Kumamoto International Foundation, Kumamoto, Japan. Vol. 39, page 3. Jan 06, 2003.
Article in 1st Biochemistry Olympiad Souvenir
The Prospects of Biochemistry and Biotechnology. Dr. Abdul Khaleque and Wisam Babar, Department of Life Sciences, North South University, Dhaka. Biochemistry Olympiad, Dhaka, Bangladesh. pp 23-27. January 08, 2010.
Article in 2nd Biochemistry Olympiad Souvenir
Biochemistry is the Heart of Life Sciences. Dr. Abdul Khaleque, Department of Life Sciences, North South University, Dhaka. 2nd Biochemistry Olympiad, Dhaka, Bangladesh. pp 22-23. Dec 24, 2010.
Article in AGM 2011 of of Bangladesh Society for Pharmaceutical Professionals (BSPP).
Biotechnology and its application in the pharmaceutical industries in Bangladesh. Dr. Abdul Khaleque, Dept. of Life Sciences, North South University, Dhaka. AGM 2011 of BSPP.
Abstracts/Conference attended (2000-2013)
1. South East Asian Public Health Education Institutes Network (SEAPHEIN) and World Health Organization South-East Asia Regional Office (WHO SEARO) Regional Meeting on emerging infectious diseases. Colombo, Sri Lanka. October 8-12, 2013
2. Md Abdul Khaleque. Fundamental Need to Advance Biosafety Biosecurity Knowledge in Bangladesh. 1st International Conference in Bangladesh on Biosafety & Biosecurity in Developing Countries. TSC, Dhaka University & Millon Hall, BSMMU, Dhaka, Bangladesh. December 7-8, 2012. pp.46
3. Md Abdul Khaleque, Ajit Bharti and Stuart K. Calderwood. Heregulin induces heat shock protein in an HSF1 dependent manner in cancer cells and leads to protection from apoptosis. 4th International Congress of Molecular Medicine.Yeditepe University, Istanbul, Turkey. June 26-30, 2011.
4. Md Abdul Khaleque, Mohammed Arif, Sajib Chakraborty, Faizule Hassan, Yearul Kabir, SAM Khairul Bashar. Lead dose-dependently induces memory impairment, DNA damage and lipid peroxidation in rat. 32nd Annual Meeting of the Japan Neuroscience Society. Nagoya, Japan. Sept 16-18, 2009
5. Nusrat Jahan, Khairul Azam, SAM Khairul Bashar , Mohammad Bakhtiar Hossain and Md Abdul Khaleque. Analysis of b-Carotene (provitamin A) from Composite Food Samples Containing OFSP and WFSP. National Seminar on “Oxygen- A friend & foe”. North South University, Dhaka, Bangladesh. 1 December, 2008
6. Genomic analysis of Ovine Herpes virus2- a bioinformatics approach. Maqsud Hossain, SAM Khairul Bashar, Md Abdul Khaleque and George Russell. National Seminar on “Oxygen- A friend & foe”. North South University, Dhaka, Bangladesh. 1 December, 2008