Consensus sequences are based on the aligning of the following sigma factors.
Cyanobacteria
SigA:
Chroococcales
Crocosphaera watsonii WH 8501 (ZP_00514786)
Microcystis aeruginosa (P52322)
Microcystis wesenbergii (BAC21133)
Synechococcus sp. PCC 7002 (AAB41506)
Synechocystis sp. PCC 6803 (NP_442860)
Thermosynechococcus elongatus BP-1 (BAC08169)
Gloeobacteria
Gloeobacter violaceus PCC7421 (BAC90513)
Nostocales
Anabaena sp. PCC7120 (all5263)
Anabaena planctonica NIES-810 (BAC44902)
Calothrix brevissima (BAC21130)
Nodularia sp. PCC 73104 (BAC21144)
Nostoc punctiforme (AAF73062)
Scytonema sp. IAM M-262 (BAC44906)
Oscillatoriales
Arthrospira platensis/ Spirulina platensis (BAC21127)
Leptolyngbya sp. PCC 7375 (BAC21147)
Oscillatoria sp. PCC 7112 (BAC21141)
Phormidium sp. IAM M-99 (BAC21137)
Pseudanabaena sp. PCC 7367 (BAC21138)
Trichodesmium erythraeum IMS101 (ZP_00674816)
Pleurocapsales
Pleurocapsa sp. PCC 7314 (BAC21145)
Pleurocapsa sp. PCC 7327 (BAC21146)
Stanieria cyanosphaera PCC 7437 (BAC21139)
Xenococcus sp. PCC 7307 (BAC21142)
Prochlorales
Prochlorococcus marinus str. MIT 9313 (NP_895100)
Stigonematales - Chlorogloeopsis fritschii PCC 6912 (BAC44901)
Fischerella major NIES 592 (BAC44904)
Plants
Sig1:
P. patens (BAB62007)
A. thaliana (BAA22214)
S. alba (CAA75859)
Nicotiana tabacum 1A (BAA88921) and 1B (BAA88922)
O. sativa (BAA24364)
Triticum aestivum (CAA10725 )
Sorghum bicolour (CAA74653)
Z. mays 1a (AAC78467) and 1b (AAC78468)
Sig2:
P. patens (BAB62008 )
A. thaliana (BAA24825)
S. alba (CAB83106 )
Spinacia oleracea (CAD20831 )
O. sativa 2A (BAC78528) and 2B (BAC78529)
Z. mays 2A (AAD17854) and 2B (AAD17855)
Sig3:
A. thaliana (BAA22215)
S. alba (CAB83107 )
S. oleracea (CAD20832 )
Z. mays (AAM12034)
Sig5:
A. thaliana (BAA78109)
P. patens (BAD80742 )
O. sativa (BAC76607)
Sig6:
A. thaliana (BAA92288)
O. sativa (BAC76602 )
Z. mays (AAD17856)
Symbols used in consensus sequences:
capital letters stand for highly conservative amino acids in orthologous genes,
small letters – low conservative amino acids,
blank – non-conservative amino acids,
dash – break of sequence.
When constructing consensus sequences I took into account taxonomy positions of organisms whose sigma factors were aligned. Besides in some unclear cases I compared positions being questionable in one consensus sequence with homologous stretches in other consensus sequences.
SigA
101 150
Anabaena SigA (39) LADDGD------IDEFLEPQTDEDDAKSGKAAKSRR-RTQSKKKHYTE
SigA Nostoc punctiforme (39) IIDEG------DDEFLEPQSDEDDAKSGKAAKSRR-RTQSKKKHYTE
Synechocystis SigA (40) DLEQEV------AATEGKEKKVRKIR--KDAVKKKPYTE
Synechococcus SigA (36) ELTLP------T-KKSRKAKTSRRKETATKKKPYTE
SigA Thermosynechococcus elongatus (54) LSDYGLDAVEIEEEESEDFEEVSDDLDGKPTKGRASRR-RTQVKKKHYTE
SigA Crocosphaera watsonii (34) EPET------KKTRKISASRR-RDPSKKKPYTE
SigA Microcystis aeruginosa (35) LEETSD------SNDGKKTRRLASARR-RDAARKKPYTE
SigA Trichodesmium erythraeum (37) LIGDR------EEYIDAQSDEDDLKSGKVAKSRT-RTAGKKKHYTE
SigA Gloeobacter violaceus (34) QAAEA------ADAAAARPARR-RSRAQRAHYTE
SigA Prochlorococcus marinus (101) QLLATAATSAPKASAETESSQAAAKATSEADAKARALANIKIGPKGVYTE
Consensus (101) d ks k kSRr rt kKkhYTE
151 200
Anabaena SigA (80) DSIRLYLQEIGRIRLLRADEEIELARKIADLLELERVRERLSEKL-----
SigA Nostoc punctiforme (79) DSIRLYLQEIGRIRLLRADEEIELARKIADLLELERVRERLSEQL-----
Synechocystis SigA (71) DSIRIYLQEIGRIRLLRAEEEIELARQIADLLELELIRDNLTLQLERQPS
Synechococcus SigA (65) DSIRIYLQEIGRIRLLRAEEEIELARKIADLLELERMREQLTEHE-----
SigA Thermosynechococcus elongatus (103) DSIRLYLQEIGRIRLLRADEEIELARKIADLLEMERVRDRLCQELG----
SigA Crocosphaera watsonii (60) DSIRVYLQEIGRIRLLRAEEEIELARKIADLLQLERIREALWEELDQEPN
SigA Microcystis aeruginosa (67) DSIRIYLQEIGRIRLLRAEEEIELARKIADLLKLERIREDFCLYSDAE—
SigA Trichodesmium erythraeum (76) DSIRLYLQEIGRIRLLRADEEIELARKIADLLELERIREELIYHL-----
SigA Gloeobacter violaceus (61) DSIRVYLQEIGRIRLLRADEEIELARQIADLLTLERLRARMGAAG-----
SigA Prochlorococcus marinus (151) DSIRVYLQEIGRIRLLRPDEEIELARKIADLLHLEELAEQFNSDHG----
Consensus (151) DSIRLYLQEIGRIRLLRADEEIELARKIADLLeLERIRE l l-----
201 250
Anabaena SigA (125) ------ERDPR------DSEWAE
SigA Nostoc punctiforme (124) ------DRDPR------DSEWAE
Synechocystis SigA (121) ELEWGKQVWKLETAKQRLVGDKKKEPKKKDIDSYLANPDNELSLENEWSQ
Synechococcus SigA (103) ------SRVP------TDKEWAE
SigA Thermosynechococcus elongatus (149) ------CTAEELERN------PEPWAK
SigA Crocosphaera watsonii (110) DDQWGHRIFQWENIEQRLCSQKNKEPKWSDIVAELNKAEIISDLEKTWKQ
SigA Microcystis aeruginosa (115) ---WGKQVFLFERIEKIIVEKSEKEPKLSDIKAYLGKTELTAPLLEEWLA
SigA Trichodesmium erythraeum (121) ------DREPQ------VSEWAN
SigA Gloeobacter violaceus (106) ------TDMHSDVADLLGDDDLLTENLLASGDQVTESEQELRRWAE
SigA Prochlorococcus marinus (197) ------HYPN------NKEWAS
Consensus (201) ------drep ------eWA
251 273
Anabaena SigA (136) AVQLPLPAFRYRLHIGRRAKDKM
SigA Nostoc punctiforme (135) AVQLPLPAFRYRLHVGRRAKDKM
Synechocystis SigA (171) QPNKNFAAFRRRLFLDRRAKDKM
Synechococcus SigA (121) AAGMPLKDFRRRLFHGRRAKDKM
SigA Thermosynechococcus elongatus (164) ELGMTVPAFRHRLFVGRKAKEKM
SigA Crocosphaera watsonii (160) QSKAYLPKFKRRLYLGRRAKDKM
SigA Microcystis aeruginosa (162) KSKEYLSAFKRRLYHGRRAKEKM
SigA Trichodesmium erythraeum (132) AVDMELPKFKRRLILGRRAKEKM
SigA Gloeobacter violaceus (146) AAGIAYEQFRDRIAYGRRAKDKM
SigA Prochlorococcus marinus (207) LVEMPNIKFRRRLMLGRRAKEKM
Consensus (251) m l FRrRL lGRRAKDKM
380 429
E coli RpoD (380) VEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFST
B subtilis SigA (139) AEANLRLVVSIAKRYVGRGMLFLDLIHEGNMGLMKAVEKFDYRKGYKFST
Anabaena SigA (159) VQSNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFST
SigA Anabaena planctonica (1) VQSNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFST
SigA Nostoc punctiforme (158) VQSNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFST
SigA Nodularia (1) ------MNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYRFST
SigA Calothrix brevissima (1) ------MNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFST
SigA Scytonema (1) ------MNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFST
Synechocystis SigA (194) VQSNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFST
Synechococcus SigA (144) VQSNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFST
SigA Thermosynechococcus elongatus (187) VQSNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFST
SigA Crocosphaera watsonii (183) VHSNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFST
SigA Microcystis aeruginosa (185) VQSNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFST
SigA Microcystis wesenbergii (1) ------MNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFST
SigA Oscillatoria (1) ------MNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFST
SigA Trichodesmium erythraeum (155) VQSNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFST
SigA Pseudanabaena (1) VQSNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFST
SigA Spirulina platensis (1) VQSNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKLDHEKGYKFST
SigA Phormidium (1) VQSNFRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFST
SigA Leptolyngbya (1) VQSNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFST
SigA Pleurocapsa (1) VHSNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFST
SigA Pleurocapsa 2 (1) ------MNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFST
SigA Xenococcus (1) VQSNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFST
SigA Stanieria cyanosphaera (1) VQSNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFST
SigA Fischerella major (1) VQSNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFST
SigA Chlorogloeopsis fritschii (1) ------MVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFST
SigA Gloeobacter violaceus (169) VQSNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFST
SigA Prochlorococcus marinus (230) VQSNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFST
Consensus (380) VQSNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFST
430 479
E coli RpoD (430) YATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQMLQEMGREPT
B subtilis SigA (189) YATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQDLGREPT
Anabaena SigA (209) YATWWIRQAITRAIADQSRTIRLPVHLYETISRIKKTTKLLSQEMGRKPT
SigA Anabaena planctonica (51) YATWWIRQAITRAIADQSRTIRLPVHLYETISRIKKTTKLLSQEMGRKPT
SigA Nostoc punctiforme (208) YATWWIRQAITRAIADQSRTIRLPVHLYETISRIKKTTKLLSQEMGRKPT
SigA Nodularia (36) YATWWIRQAITRAIADQSRTIRLPVHLYETISRIKKTTKLLSQEMGRKPT
SigA Calothrix brevissima (36) YATWWIRQAITRAIADQSRTIRLSVHLYETISRIKKTTKLLSQEMGRKPT
SigA Scytonema (36) YATWWIRQAITRAIADQSRTIRLPVHLYETISRIKKTTKLLSQEMGRKPT
Synechocystis SigA (244) YATWWIRQAITRAIADQSRTIRLPVHLYETISRIKKTTKLLSQEMRRKPT
Synechococcus SigA (194) YATWWIRQAITRAIADQSRTIRLPVHLYETISRIKKTTKILSQELGRKPT
SigA Thermosynechococcus elongatus (237) YATWWIRQAITRAIADQSRTIRLPVHLYETISRIKKTTKLLSQEMGRKPT
SigA Crocosphaera watsonii (233) YATWWIRQAITRAIADQSRTIRLPVHLYETISRIKKTTKLLSQEMRRKPT
SigA Microcystis aeruginosa (235) YATWWIRQAITRAIADQSRTIRLPVHLYETISRIKKTTKILSQEMRRKPT
SigA Microcystis wesenbergii (36) YATWWIRQAITRAIADQSRTIRLPVHLYETISRIKKTTKILSQEMRRKPT
SigA Oscillatoria (36) YATWWIRQAITRAIADQSRTIRLPVHLYETISRIKKTTKLLSQEMGRKPT
SigA Trichodesmium erythraeum (205) YATWWIRQAITRAIADQSRTIRLPVHLYETISRIKKTTKLLSQEMGRKPT
SigA Pseudanabaena (51) YATWWIRQAITRAIADQSRTIRLPVHLYETISRIKKTTKLLSQEMGRKPT
SigA Spirulina platensis (51) YATWWIRQAITRAIADQSRTIRLPVLLYETISRIKKTTKLLSQEMGRKPT
SigA Phormidium (51) YATWWIRQAITRAIADQSRTIRLPVHLYETISRIKKTTKLLSQEMGRKPT
SigA Leptolyngbya (51) YATWWIRQAITRAIADQSRTIRLPVHLYETISRIKKTTKLLSQELGRKPT
SigA Pleurocapsa (51) YATWWIRQAITRAIADQSRTIRLPVHLYETISRIKKTTKLLSQERGRKPT
SigA Pleurocapsa 2 (36) YATWWIRQAITRAIADQSRTIRLPVHLYETISRIKKTTKILSQEMRRKPT
SigA Xenococcus (51) YATWWIRQAITRAIADQSRTIRLPVHLYETISRIKKTTKLLSQERGRKPT
SigA Stanieria cyanosphaera (51) YATWWIRQAITRAIADQSRTIRLPVHLYETISRIKKTTKLLSQEMGRKPT
SigA Fischerella major (51) YATWWIRQAITRAIADQSRTIRLPVHLYETISRIKKTTKLLSQEMGRKPT
SigA Chlorogloeopsis fritschii (45) YATWWIRQAITRAIADQSRTIRLPVHLYETISRIKKTTKLLSQEMGRKPT
SigA Gloeobacter violaceus (219) YATWWIRQAITRAIADQSRTIRLPVHLYETISRIKKTTKLLSQELGRKPT
SigA Prochlorococcus marinus (280) YATWWIRQAITRAIADQSRTIRLPVHLYETISRIKKTTKVLSQEFGRKPT
Consensus (430) YATWWIRQAITRAIADQSRTIRLPVHLYETISRIKKTTKLLSQEMGRKPT
530 579
E coli RpoD (530) LPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGIDMNTDHTLEEVGKQ
B subtilis SigA (289) SPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGKV
Anabaena SigA (309) -PEDQVSKNLLREDLEKVLDSLSPRERDVLRLRYGLDDGRMKTLEEIGQI
SigA Anabaena planctonica (151) -PEDQVSKNLLREDLEKVLDSLGPRERDVLRLRYGLDDGRMKTLEEIGQI
SigA Nostoc punctiforme (308) -PEDQVSKNLLREDLEKVLDSLSPRERDVLRLRYGLDDGRMKTLEEIGQI
SigA Nodularia (136) -PEDQVSKNLLREDLEKVLDSLSPRERDVLRLRYGLDDGRMKTLEEIGQI
SigA Calothrix brevissima (136) -PEDQVSKNLLREDLEKVLDSLSPRERDVLRLRYGLGDGRMKTLEEIGQI
SigA Scytonema (136) -PEDQVSKNLLREDLEKVLDSLSPRERDVLRLRYGLDDGRMKTLEEIGQI
Synechocystis SigA (344) -PEDEVSKNLLREDLENVLDTLSPRERDVLRLRYGLDDGRMKTLEEIGQI
Synechococcus SigA (294) -PEDQVSKSLLREDLENVLDTLSARERDVLRLRYGLDDGRMKTLEEIGQI
SigA Thermosynechococcus elongatus (337) -PEDQVSKHLLREDLETVLCTLSPRERDVLKLRYGLDDGRMKTLEEIGQL
SigA Crocosphaera watsonii (333) -PEDEVSKNLLREDLENVLDTLSPRERDVLRLRYGLDDGRMKTLEEIGQI
SigA Microcystis aeruginosa (335) -PEDEVSKNLLREDLENVLDTLSPRERDVLRLRYGLDDGRMKTLEEIGQI
SigA Microcystis wesenbergii (136) -PEDEVSKNLLREDLENVLDTLSPRERDVLRLRYGLDDGRMKTLEEIGQI
SigA Oscillatoria (136) -PEDQVSKNLLREDLESVLDTLSPRERDVLRLRYGLDDGRMKTLEEIGQI
SigA Trichodesmium erythraeum (305) -PEDEVSKNLLREDLESVLNSLSPRERDVLRLRYGLDDGRMKTLEEIGQI
SigA Pseudanabaena (151) -PDDQVAKNLLREDLENVLGTLSPRERDVLKLRYGLDDGRMKTLEEIGQI
SigA Spirulina platensis (151) -PEDQVSKNLLREDLESVLDTLSPRERDVLRLRYGLDDGRMKTLEEIGQI
SigA Phormidium (151) -PEDQVSKNLLREDLEGVLATLSPREKDVLRLHYGLDDGRMKTLEEIGQI
SigA Leptolyngbya (151) -PEDEVSKSLLREDLEGVLSTLSPSERDVLRLRYGLDDGRMKTLEEIGQI
SigA Pleurocapsa (151) -PEDQVSKSLLREDLESVLDTLSPRERDVLRLRYGLDDGRMKTLEEIGQI
SigA Pleurocapsa 2 (136) -PEDEVSKNLLREDLENVLDTLSPRERDVLRLRYGLDDGRMKTLEEIGQI
SigA Xenococcus (151) -PEDQVSKSLLREDLESVLDTLSPRERDVLRLRYGLDDGRMKTLEEIGQI
SigA Stanieria cyanosphaera (151) -PEDQVSKSLLREDLENVLDTLSPSERDVLRLRYGLDDGRMKTLEEIGQI
SigA Fischerella major (151) -PEDQVSKNLLREDLEKVLDSLSPRERDVLRLRYGLDDGRMKTLEEIGQI
SigA Chlorogloeopsis fritschii (145) -PEDQVSKNLLREDLEKVLDSLSPRERDVLRLRYGLDDGRMKTLEEIGQI
SigA Gloeobacter violaceus (319) -PEDKVAKNLLREDLEAVLNTLSPRERDVLRLRYGLDDGRMKTLEEIGQL
SigA Prochlorococcus marinus (380) -PEQDVAKNLLREDLEGVLATLSPRERDVLRLRYGLDDGRMKTLEEIGQI
Consensus (530) -PEDQVSKNLLREDLE VLDTLSPRERDVLRLRYGLDDGRMKTLEEIGQI
580 613
E coli RpoD (580) FDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD
B subtilis SigA (339) FGVTRERIRQIEAKALRKLRHPSRSKRLKDFLE-
Anabaena SigA (358) FNVTRERIRQIEAKALRKLRHPNRNSVLKEYIR-
SigA Anabaena planctonica (200) FNVT------
SigA Nostoc punctiforme (357) FNVTRERIRQIEAKALRKLRHPNRNSVLKEYIR-
SigA Nodularia (185) FNVT------
SigA Calothrix brevissima (185) FNVT------
SigA Scytonema (185) FNVT------
Synechocystis SigA (393) FNVTRERIRQIEAKALRKLRHPNRNSILKEYIR-
Synechococcus SigA (343) FNVTRERIRQIEAKALRKLRHPNRNSILKEYIR-
SigA Thermosynechococcus elongatus (386) FNVTRERIRQIEAKALRKLRHPNRNSVLKEYIR-
SigA Crocosphaera watsonii (382) FNVTRERIRQIEAKALRKLRHPNRNSILKEYIR-
SigA Microcystis aeruginosa (384) FNVTRERIRQIEAKALRKLRHPNRNSILKEYIR-
SigA Microcystis wesenbergii (185) FNVT------
SigA Oscillatoria (185) FNVT------
SigA Trichodesmium erythraeum (354) FNVTRERIRQIEAKALRKLRHPNRNSILKEYIR-
SigA Pseudanabaena (200) FNVT------
SigA Spirulina platensis (200) FNVT------
SigA Phormidium (200) FNVT------
SigA Leptolyngbya (200) FNVT------
SigA Pleurocapsa (200) FNVT------
SigA Pleurocapsa 2 (185) FNVT------
SigA Xenococcus (200) FNVT------
SigA Stanieria cyanosphaera (200) FNVT------
SigA Fischerella major (200) FNVT------
SigA Chlorogloeopsis fritschii (194) FNVTR------
SigA Gloeobacter violaceus (368) FNVTRERIRQIEAKALRKLRHPNRNSVLKEYIR-
SigA Prochlorococcus marinus (429) FEVTRERIRQIEAKALRKLRHPNRNGVLKEYIK-
Consensus (580) FNVTRERIRQIEAKALRKLR
Sig1
331 380
AtSig1 (173) THVKAVSDVSSG------KQVRGYVKGVISEDVLSHVEVVRLSK
SaSig1 (154) KAVSEVNFQNN------LVKGYVKGVISDHVLSHAEVVRLSK
NtSig1A (167) RHSSATPTSAAKPLRSIIGPELLQNRLKGYVKGVVSEELLTHAEVLQLSK
NtSig1B (161) PKNSATSDSSSKRLRSVIGPELLQNRSKGYINGVVGQELLTHTEVVQLSK
PpSig1 (331) LTIHPLKKYQKGERVRRVKFDGVQDYLTTYLREITKINLLTKQEEVFLSK
OsSig1 (187) KN------G--AAHFAVSPELIQSRNRIYLRGTVSKELLTHKQVVHLSH
TaSig1 (127) KK------GSGAVHLSISPELLQSRNRIYLRGTVSKELLTQKQVVHLSK
SbSig1 (168) ------GPGRKSVTVSPELMQSRNRIYLRGTVSKDLLTHKQVIQLSR
ZmSig1a (66) ------FVGRKSVTVSPELMQSRNRIYLRGTVSKDLLTHKQVVQLSR
ZmSig1b (21) ------KSVAISPELMQSRNRIYLRGTVSKDLLTHKQVIQLSR
Consensus (331) i pellq r r YLRg vs dLLTh qVVqLSK
381 430
AtSig1 (211) KIKSGLRLDDHKSRLKDRLGCEPSDEQLAVSLKISRAELQAWLMECHLAR
SaSig1 (190) KIKSGLRLDEHKSRLTDRLGCEPSDEQLAMSLKISRAELQAWLMECHLAR
NtSig1A (217) KIQVGLYLEEQKSRLKERLGCEPSDDQLAVSLKMSRTDLQSTLIECSLAR
NtSig1B (211) KIKFGLYLEEQKSRLKERLGCEPSDDQLAISLKISRADLRSTLIECSLAR
PpSig1 (381) KLRIGLNLLEERKKLAAELGYVPSNEEWAPHMNLSVGDLVRKLGEAEWAR
OsSig1 (228) KIKDGIWLQQQRSKLKEKLGNEPSYKQLAHSLKISPPELRSRMHESFLAR
TaSig1 (170) KIKDGIWLQQQRSKLKEKLGNEPSYKQMAQSLRISTPELRSRMRESFLAR
SbSig1 (209) KIKDGIWLQHQRSKLKEKLGNEPSYKQLAQSLHISPPELRARMRESFLAR
ZmSig1a (107) KIKDGIWLQHQRSKLKEKLGNEPSYKQLAQSLRIPAPELRARMRESFLAR
ZmSig1b (58) KIKDGIWLQHQRSKLKEKLGNEPSYKQLAQSLRISAPELRARMRESFLAR
Consensus (381) KIK GI LqeqRsKLkekLG ePS qlA sLkIS EL a L Es lAR
431 480
AtSig1 (261) EKLAMSNVRLVMSIAQRYDNLGAEMSDLVQGGLIGLLRGIEKFDSSKGFR
SaSig1 (240) EKLAMSNVRLVMSIAQRYDNMGAEMSDLVQGGLIGLLRGIEKFDSSKGFR
NtSig1A (267) ERLAMSNVRLVMSIAQRYDNVGAEMADLVQGGLIGLLRGIEKFDPSKGYK
NtSig1B (261) EKLAMSNVRLVMSIAQRYDTNGTEMADLIQGGLIGLLRGIEKFDHSRGFK
PpSig1 (431) DKMVMANLRLVVSVAKQYHSFGLEMADLIQEGSIGLLRGVEKFDHTRGYK
OsSig1 (278) EMLTMSNLRLVISIAQKYDNLGVELADLIQGGLIGLLRGIEKFDASRGFR
TaSig1 (220) EVLTMSNIRLVISIAQKYDKLGVELSDLIQGGLIGLLRGIEKFDASRGFR
SbSig1 (259) EMLTMSNIRLVISIAQKYDNLGVELADLIQGGLIGLLRGIEKFDASRGFR
ZmSig1a (157) EMLAMSNTRLVISIAQKYDNLGVELPDLIQGGLIGLLRGIEKFDASRGFR
ZmSig1b (108) EMLTMSNIRLVISIAHKYDNLGVDLADLIQGGLIGLLRGIEKFDASRGFR
Consensus (431) E L MSNVRLVISIAqkYdnlG ELADLIQgGlIGLLRGIEKFDaSRGFR
481 530
AtSig1 (311) ISTYVYWWIRQGVSRALVDNSRTLRLPTHLHERLGLIRNAKLRLQEKGIT
SaSig1 (290) ISTYVYWWIRQGVSRALVDNSRTLRLPTHLHERLGLIRNAKLRLQEKGIT
NtSig1A (317) ISTYVYWWIRQGVSRALVENSRTLRLPTYLHERLSLIRNAKARLEEKGIT
NtSig1B (311) ISTYVYWWIRQGVSRALVENSRTLRLPIHLHERLSLIRNAKIKLKENGIT
PpSig1 (481) LSTYVHWWIRQGVTRAIADHSRTVRLPVHVHDTLGRIRKAKSTLLSEGCT
OsSig1 (328) ISTYVYWWIRQGVSRALAENSKTFRLPTYLHERLIAIRGAKYELEDQGIA
TaSig1 (270) ISTYVYWWIRQGVSRALADNSKTFRLPTYLHERLIAIRGAKYALEDQGVS
SbSig1 (309) ISTYVYWWIRQGVSRALADNSKAFRLPTYLHERLIAIRGAKYALEDQGIA
ZmSig1a (207) ISTYVYWWIRQGVSRALADNSRSFRLPTYLHERLVAIRGAKYALEDQGIA
ZmSig1b (158) ISTYVYWWIRQGVSRALADNSKTFRLPTYLHERLLAIRGAKYALEDQGIA
Consensus (481) ISTYVYWWIRQGVSRALADnSRTlRLPtHLHERL IR AK L dqGi
531 580
AtSig1 (361) PSIDRIAESLNMSQKKVRNATEAVSKVFSLDRDAFP-SLNGLPGETHHSY
SaSig1 (340) PSIDRIAESLNMSQKKVRNATEAVSKIFSLDRDAFP-SLNGLPGETHHSY
NtSig1A (367) PSVNNIAASLNMSQKKVRNATEASSKMYSLDREAFP-SLNGLPGETLHSY
NtSig1B (361) PSIEKIAECLNMSQKKVRNATEASSKVFSLDREAFP-SLNGLPGETLHSY
PpSig1 (531) ASVQEISEATNLTGTKIKNTLQVTKKLVSLDKEIGRNTLQSGDGETLHSL
OsSig1 (378) PTIENIAGSLNISEKKVLNATEAVNKVLSLDQQAFP-SLNGLPGETLHSY
TaSig1 (320) PTTQNIAKLLNISEKKVHNATEAVNKALSLDQQAFP-SLNGLPGDTLHSY
SbSig1 (359) PTVENIAESLNISEKKVHNATEAVNKVLSLDQQAFP-SLNGLPGDTLHSY
ZmSig1a (257) PTVENIAESLNISEKKVHNATEAVHKVISLDQQAFP-SLNGLPGDTLHSY
ZmSig1b (208) PTIENIAESLNISVKKVHNATEAVHKVLSLDQQAFP-SLNGLPGDTLHSY
Consensus (531) pSIenIAeslNIS kKVrNateav KVlSLDqqafp-SLNglpGETlHSy
581 630
AtSig1 (410) IADTRLENNPWHGYDDLALKEEVSKLISATLGEREKEIIRLYYGLDKEC-
SaSig1 (389) IADNRLENNPWHGYDALALKDEVSKLISVTLGEREREIIRLYYGLDKEC-
NtSig1A (416) IADNHLENNPWHGVDVWALKDEVNNLISSTLRDREREIIRLYYGLDNEC-
NtSig1B (410) IADNRLENIPWHGVDEWMLKDEVNNLISSTLREREREIVRLYYGLDNEC-
PpSig1 (581) VADRDIENQPWTIVERMLMKDDVDKLLTSQLGPRERDIVRLHYGVGREDG
OsSig1 (427) IEDQNVANDPWHGFEEWYLKEEVNKLLNSTLNERERDIIRLYHGIGKQC-
TaSig1 (369) IEDQNVANDPWHGFEEWYLKEEVNKLLNSNLTERERDIIQLYHGIGKQC-
SbSig1 (408) IEDQNVANDPWHGFEERYLKEEVNNLINSNLNERERDIIRLYHGIGKQC-
ZmSig1a (306) IEDQNVANDPWHGFEERYLKEEVNSLINSTLNERERDIIRLYHGIGKQC-
ZmSig1b (257) IEDQNVANDPWHGFEEMYLKEEVNNLINSNLNERERDIIRLYHGIGKQC-
Consensus (581) I Dq VeN PWhg Ee LKEEVnkLI stL eRERDIIRLYHGIgkec-
631 677
AtSig1 (459) --LTWEDISKRIGLSRERVRQVGLVALEKLKHAARKRKMEAMILKN-
SaSig1 (438) --LTWEDISQRIGLSRERVRQVGLVALEKLKHAARKRKMEAMILKN-
NtSig1A (465) --LTWEDISKRIGLSRERVRQVGLVALEKLKHAARKRQLDAMLIKQ-
NtSig1B (459) --LTWEDISRRIGLSRERVRQVGLVALEKLKHAARKRRLDAMLVKH-
PpSig1 (631) SVMSLESISYRYGVSRERVRQLETAAMRKLQIKSREQGLDRYLHSMQ
OsSig1 (476) --HTWEDISRQFGLSRERVRQVGLIAMEKLKHAARRKNLEALLEDY-
TaSig1 (418) --HTWEDISRQFGLSRERVRQVGLIAMEKLKQRARRKKLDALLQDY-
SbSig1 (457) --HTWEDISRQFGLSRERVRQVGLVAMEKLKHAARRKHLDALLEEY-
ZmSig1a (355) --HTWEDISRQFGLSRERVRQVGLVAMEKLKHAARREHLDALLEEY-
ZmSig1b (306) --HTWEDISRQFGLSRERVRQVRLVAMEKLKHAARRKHLNALLEDY-
Consensus (631) -- TWEDISrrfGLSRERVRQVglvAMeKLKhaARRK LDalL
Sig2
251 300
AtSig2 (232) ASGIP-SVKTGSSPKKKRLVAQEVDHNDPLRYLRMTTSSSKLLTVREEHE
SaSig2 (231) ASGMQSSVKTGSSSRKKRVASQEIDHNDPLRYLRMTTSSSKLLTAREEQQ
SoSig2 (165) ASNVV-SVKVGSNSRKKRTSVQEVDYSDPLRYLRGATNSSRLLTSSEETE
PpSig2 (236) EKVATDLASAPPLPKPKKPQLAADPSDPVRAYLRDIG-RTKLLTAREEVD
OsSig2A (224) ATIVRTSPKPATSSKKKRSKGASSGTNPLGSLWKMTG--RRLLTAKEEVE
ZmSig2A (199) STTLHASQKVALSSKKKRSKGSSSSMNPLGSLWKMTG--RRLLTAKEEVE
OsSig2B (201) AKSFSG-RNPVAASSSARKKRLKGCRSPLGCFYKMTG--RRLLTAKQEVE
ZmSig2B (189) AKCSSS-MKPVGASSTRRK-RLKGCLTPLGCLYKMTG--PRLLTAEQEVE
Consensus (251) as s k sskkKR Kmtg-- RLLTAkEEvE
301 350
AtSig2 (281) LSAGIQDLLKLERLQTELTERSGRQPTFAQWASAAGVDQKSLRQRIHHGT
SaSig2 (281) LSEGIQDLLKLERLQAELTERCGHQPTFSQWASAAGVDKKTLRKRITHGT
SoSig2 (214) LSAGIQDLLKLEKLHDELKVRFGKSPSFVQWAAAAGIDQRTLRRRLNHGT
PpSig2 (285) LSHQIQDLLKLENIKSNLEREIGRNATIGEWSRAVGMEQNAFEARLKKGR
OsSig2A (272) FSEGIQDLLKLEAIQAELAEYNGGQPTFAQWATAAGVDERTLRKRLDHGI
ZmSig2A (247) FSEGIQDLLKLEAIQAELIEYNGGQPTFSQWATAAGVDEKTLRKRLNYGI
OsSig2B (248) FSQGIQDLLKLEAIQKELAHYNGGEPTFSQWAEAAGTDENTLRKRLNYGI
ZmSig2B (235) FSEGIQDLLKLEAIQKEVAQYNAGEPTFSQWAAAAGTDENTLRRRLNHGV
Consensus (301) lSegIQDLLKLE Iq EL G qpTFsQWAsAAGvDekTLRkRLnHG
351 400
AtSig2 (331) LCKDKMIKSNIRLVISIAKNYQGAGMNLQDLVQEGCRGLVRGAEKFDATK
SaSig2 (331) QCKDRMIKSNIRLVISIAKNYQGAGMDFQDLVQEGCRGLVRGAEKFDATK
SoSig2 (264) FCKDKMIKSNVRLVISIAKNYQGSGMNLQDLVQEGCRGLVRGAEKFDASK
PpSig2 (335) FAKDKMVNSNLRLVVSIAKNYQGRGMTLQDLIQEGSMGLVRGAEKFDPTK
OsSig2A (322) YCKNRMVTSNVRLVISIAREFEGPGMELYDLIQEGMQGLIRGAEKFDASK
ZmSig2A (297) YCKNRMVTSNVRLVISIAREFEGPGMDFYDLIQEGMQGLIRGAEKFDSSK
OsSig2B (298) SCKNTMVKSNVRLVISIAREFEGPGMEFSDLIQEGIQGLVRGAEKFDASK
ZmSig2B (285) YCKNRMVKSNVRLVISIAKEHEGPGMEFSDLIQDGMQGLIRGAEKFDASK
Consensus (351) fcKnRMVkSNVRLVISIAKnYqG GMel DLIQEG GLVRGAEKFDaSK
401 450
AtSig2 (381) GFKFSTYAHWWIKQAVRKSLSDQSRMIRLPFHMVEATYRVKEARKQLYSE
SaSig2 (381) GFKFSTYAHWWIKQAVRKSLSDQSRMIRLPFHMVEATYRVKEARKQLYSE
SoSig2 (314) GFKFSTYAHWWIKQAVRKSLSDQSRTIRLPFHMVEATYRVKEARKQLLSE
PpSig2 (385) GFKFSTYAHWWIRQAVTRSIADQSRTIRLPIHLYEVISRINKAKRMLVQE
OsSig2A (372) GFRFSTYSHWWIKQAMRKSVSEQSQIFRLPAHMVEASYRVKECIKRLRRK
ZmSig2A (347) GFRFSTYSHWWIKQAMRKSVSEQSQIFRLPAHMVEASYRVKECTKRLRRK
OsSig2B (348) GFRFSTYSHWWIKQAIRKSVLEQTQIIRLPAHMAEASSRVKECRRRLRRQ
ZmSig2B (335) GFRFSTYSHWWIKQAIRKSVLEQTQIIRLPAHMAEASSRVKECCRRLGRE
Consensus (401) GFKFSTYAHWWIKQAVrKSVsDQSriiRLP HMvEAsyRVkearK L e
451 500
AtSig2 (431) TGKHPKNEEIAEATGLSMKRLMAVLLSPKPPRSLDQKIGMNQNLKPSEVI
SaSig2 (431) TGKQPKNEEVAEATGLSMKRLMAVLLSPKPPRSLDQKIGINLNLKPSEVI
SoSig2 (364) NGKQPNNEEVAEATGLSMKRLTAVLMTPKAPRSLDQKIGFNMNLKPSEVI
PpSig2 (435) HGREARNEEVAELVGLTVEKLKSVVKSAKAPGSMERPIGKDGDTTLGELV
OsSig2A (422) LKRRPTNEEIAADTGMPIKRVEAAVNLPKYSVSLDSKIGS-TDMTYQEVT
ZmSig2A (397) LRRRPTNEEIAVDTGIPIKRVEAAVNLPKYSVSLDSKIGS-TDMTYQEVT
OsSig2B (398) LKRLPTNEEIASDTGMPLRRVEAAMSLPKYTVSLTSKVGC-TDMTYQEIT
ZmSig2B (385) LKRLPSNEEIAVDTGMTIRRVEAAMSLPKYSVSFTSKVGC-TDVTYQEIM
Consensus (451) R p NEEIA tGLsIkRL AvL pK SLd kIG l EVi
501 550
AtSig2 (481) ADPEAVTSEDILIKEFMRQDLDKVLDSLGTREKQVIRWRFGMEDGRMKTL
SaSig2 (481) ADPEAETSEDILMKQFMRQDLDKVLDSLSTREKQVIRWRFGMEDGRMKTL
SoSig2 (414) ADPEAETAEDLLTKQFMRQDLEKVLDTLSSREKQVIRWRFGLEDGRMKTL
PpSig2 (485) ADTDVDSPEDAIVKQLMRQDIEGVLRTLNPREREVLRLRFGLDDGRSKTL
OsSig2A (471) ADPTAETAEEMLNRLSMKRDVHKALDTLTTRERQVVTLRFGLEDGRIRTL
ZmSig2A (446) ADPSAETAEEMLNRMSMKKDVHMALDTLTTREKQVVVLRFGLEDGRIRTL
OsSig2B (447) PDTSTETAEEMLHRWLMKEDVDRALDGLSPRERQVIRYRFGMDDGRLRTL
ZmSig2B (434) PDTSAETAEETLHRWLMKKDVNTALDSLSPRKKQVMRYRFGIEGGRARTL
Consensus (501) aDp aETaEDmL K MK DVd vLdtLstREKqVIR RFGLEDGRmKTL
551 595
AtSig2 (531) QEIGEMMGVSRERVRQIESSAFRKLKNKKRNNHLQQYLVAQS---
SaSig2 (531) QEIGETMGVSRERVRQIDTSAFRKLKNKKRNSHLQQYLVAQTKES
SoSig2 (464) QEIGELMGVSRERIRQIESSAFRKLQNKKKTINLQQYMFL-----
PpSig2 (535) EEIGQIFKVTRERIRQIEAKAMRKLRQPSRNSILREYLDVKSDAI
OsSig2A (521) QEIGNTMGVSRERIRQIESAAFRKLRSKKRVNALKDYLVPVGNW-
ZmSig2A (496) QEIGNIMGVSRERIRQIESGAFRKLRSKKKVKALKDYLVPVGNW-
OsSig2B (497) HDIGRLMGVSRERIRQIELVAFRKLRGRKKVQSLQHYLQPVESW-
ZmSig2B (484) HDIGQLMGVSRERIRQIELGAFRKLRSKNRLQSLQHYLRPAESW-
Consensus (551) qEIG lmgVSRERIRQIEs AfRKLR kkR Lq YL
Sig3
251 300
AtSig3 (234) S--VTAENGDQSSLPIGLRTTWNNIDVPRVRRPPKYRKK---RERISRNE
SaSig3 (235) ------NGDQGSSTISSRTTWEGIDVPRVRKPSKYRKK---RERISRNE
SoSig3 (211) RRLQASTSKRCACKSESSIESSIHHVVSTAAKKTKARGR---RAIIAKNE
ZmSig3 (229) RASSDEKGSRTSVKVNPPRRPKKSRKASSSQFISEWKSHPGQRRIIVREQ
Consensus (251) n r s s s r T vs k SKyR K---R IsRNE
301 350
AtSig3 (279) TEMSTGVKIVADMERIRTQLEEESGKVASLSCWAAAAGMNEKLLMRNLHY
SaSig3 (275) AEMSQGVKIVADMERIRTKLEEETGKVASMSCWAEAAGMNQKLLMHNLHF
SoSig3 (258) AEMSRGIKVVAQLEKVKLTLEKETGKAVSLNCWAEAAGIDEKVLQHDLHF
ZmSig3 (279) SALLSTIKECAGLEKIREKMVKE-GQEVSYHRWAEAAGVDEAELVSRLQA
Consensus (301) AeMs gIKivAdLEKIR kLekEtGK vSl cWAeAAGMnEklLm nLHf
351 400
AtSig3 (329) GWYCRDELVKSTRSLVLFLARNYRGMGIAHEDLIQAGYVGVLQGAERFDH
SaSig3 (325) GWYCRDELVKSTRSLVLFLARNYRGLGIAHEDLIQAGYVGVLQGAERFDH
SoSig3 (308) GWYCRDELLKSGRSLVLYLSRYYRGLGVAHEDLMQAGNIGILQGAERFDH
ZmSig3 (328) GYCCRDRLLVTTEWLVRCIARTYTGMGTALDDLLQAGKMGVLDGAEKFDS
Consensus (351) GWyCRDELLkStRsLVlfLAR YrGLGiAhEDLIQAG VGVLQGAERFDh
401 450
AtSig3 (379) TRGYKFSTYVQYWIRKSMSTMVSRHARGVHIPSSIIRTINHIQKARKTLK
SaSig3 (375) TRGYKFSTYVRYWIRKSMSTMVSRHARGVHIPASIIRTMSQIQKARKTLK
SoSig3 (358) TRGYQFSTYVQYWIRKAMSQLVSRHGRGIKVPTTLSTTINRVQKARKILK
ZmSig3 (378) RKGCRFSTYVKYWIRKGMLALLAENSGVTLLPARMESIMRKVKEARRGIR
Consensus (401) tRGyKFSTYVqYWIRKSMs LVSRHArgv IPAsI tI kVQKARK LK
451 500
AtSig3 (429) TSHGIKYAADEEIAKLTGHSVKKIRAANQCLKVVGSIDKKVGDCFTTKFL
SaSig3 (425) TSQGIKYPADEEISKLTGYSLKKIRAANQCLKVVGSIDKKVGDCFTTRFL
SoSig3 (408) RSQG-KHPDDVEVSKSTGLSLDKIKSADNCLRIVGSLDQKVGHFINMKYL
ZmSig3 (428) YRQG-RNPSDSEVAAAVGVSVANVRLARKCSRRAVSLYSEVAIGQDVKFA
Consensus (451) sQG KypAD EIAk TG SL kIRaAnqClKvVgSLdkkVG KFl
501 550
AtSig3 (479) EFTPDTTMESPEEAVMRQSARRDIHDLLEGLEPREKQVMVLRYGLQDYRP
SaSig3 (475) EFTPDPTMESPEEAVMRQSARGEVHNLLEGLEPREKQVMVLRYGLQDYSP
SoSig3 (457) ECTPDTALEMPEKTVMKQHMRNDVHKLLQELDPKERRVLIHRYGLE-GHC
ZmSig3 (477) EVIPDESASPDEAALFRGQLRERLLLVLGRLPAREGRVLRLRHGLEDGRC
Consensus (501) E tPD TmE pE aVMRq R dVh LL LepREkrVLvlRYGLedgr
551 593
AtSig3 (529) KSLEEIGKLFKVSKEWIRKIERRAMAKLRDQPNAEDLRYYLNQ
SaSig3 (525) KSLEEIGKILRVSKEGIRKIERRAMAKLRDQPKADNLRYYLNQ
SoSig3 (506) MSLHEVGKVFDVSKEWIRKMEKRALTKLRTEQNQTNLKHYLNL
ZmSig3 (527) RSLEEIGGIYGVSKEWIRKIEKSAMARLRDDDAVRRDLHDFV-
Consensus (551) kSLEEIGkIF VSKEWIRKIEKrAMaKLRd nlrHyLn
Sig5
312 361
AtSig5 (196) LVRDYSASNDIVSLDWKKMKIPPVLSSTEHAWLFKLMQPMKALLEVKDVL
OsSig5 (180) LLREHAVSTDMGSLDWKRMKIPPVLTSAQSIRLFRIMQPMKAIMEMKENL
PpSig5 (312) LINKYTTSMDLAAPLWDKLDRG-LLTAVEENRLAVFMKPMKKLEKLQKVL
Consensus (312) LIrdY S DI S WkKMk VLTS e L iMqPMK L eLkd L
362 411
AtSig5 (246) QKSLGREPREAEIAGEINMTAGEVKKKIEIGRAARNKLIKHNLRLVLFVM
OsSig5 (230) ENEVQSEPNDAQLAEAMNMSVLQMRRHLEVGRAARNKLIKHNLRLVLYTI
PpSig5 (361) MKQLGRDPTDEEWAAAAGVDLLIMRRRLALGRAARTKLIQRNLRLVLSQA
Consensus (362) k L EP D e A inMsl MRRkL IGRAARnKLIkhNLRLVL
412 461
AtSig5 (296) NKYFHEFTNGPKFQDLCQAGMRGLITAIDRFEPKRKFRLSTYGLFWIRHA
OsSig5 (280) NKYYPDMSNDERFDDICQAGANGLITAIDRFEPKRGFRISTYALFWIRHS
PpSig5 (411) YKYHKD-SMSLSIQDLCQEGVIGLMQAVDKFDPGKGYRFSTYGIYWIRNS
Consensus (412) KYh D S qDLCQ Gm GLI AIDRFEP R FRiSTYGLFWIRhS
462 511
AtSig5 (346) IIRSMTTS-NFTRVPFGLESVRVEIYKAKTELWFEMGRPPTEDEVVERLK
OsSig5 (330) IVRAITLS-NFTRFPFAMESERQEIHRAREELAFELGRAPTEEEVMKKVG
PpSig5 (460) ILRAQTKSGHMLRSPHNVSTHRVNIQRAKVELFSELGRQPTNDEIRKHLG
Consensus (462) IIRA T S nf R Pf L S R I RAK EL ELGR PT DEV kkL
512 561
AtSig5 (395) ITPERYREVLRAAKPVLSLNSKHSVTQEEFINGITDVDGVGANNSRQPAL
OsSig5 (379) LSPARYRDVVRMTRPTYSLHARNRVTQEELINEVTDDDAIGVDTSSHNTL
PpSig5 (510) ITADRFRDILRTNMRTSSLHEHDRITGREKVENLVSEEDALDTLAGGTSM
Consensus (512) IT RYRDVLR SLh k VT eE In Itd D I S L
562 611
AtSig5 (445) LRLALDDVLDSLKPKESLVIRQRYGLDGKGDRTLGEIAGNLNISREMVRK
OsSig5 (429) LRLAIDDLLDSLKPKESVVIRQRFGLDGRGKRTLSEIAGNLNISREMVRK
PpSig5 (560) VQYGLDDVLDSLRPKENLVLRQRFGLDGKGQRSLSEVGLILNLSREMVRR
Consensus (562) Lr ALDDVLDSLKPKEsLVIRQRFGLDGKG RTLsEIA LNISREMVRK
612 634
AtSig5 (495) HEVKALMKLKHQARVDYLRQYIV
OsSig5 (479) YELKALMKLKHPTRVEYLRRYM-
PpSig5 (610) YELRGILKLQHPTRVEYLRNYLN
Consensus (612) YELKALMKLkH tRVEYLR YI
Sig6
251 300
AtSig6 (221) AKKKYKQGADNDDALQLFLWGPETKQLLTAKEEAELISHIQHLLKLEKVK
OsSig6 (247) KSKKYGRVLEPDEPFKLFLRDRETTEFLTAKEERHLFSKIQILMKIEEAH
ZmSig6 (217) --DKFGRVLIQTSLFRLFLRDRETTEFLTAKEEKQMFSQIQNLMKLEGAQ
Consensus (251) kKY r d fkLFL d ETtefLTAKEE LfS IQ LMKLE a
301 350
AtSig6 (271) TKLESQNGCEPTIGEWAEAMGISSPVLKSDIHRGRSSREKLITANLRLVV
OsSig6 (297) RKLEVQCGREPTLAEWAEAVGMSSKELQSSIRTGRRCREKMARSNFRLVI
ZmSig6 (265) RNLQAQCGREPTVEEWAQAVGMSCRELQSCVRTGRRCREKMARSNFRLVI
Consensus (301) kLEaQ G EPTI EWAeAVGMSs LqS Ir GR cREKM SNfRLVI
351 400
AtSig6 (321) HIAKQYQNRGLNFQDLLQEGSMGLMKSVEKFKPQSGCRFATYAYWWIRQS
OsSig6 (347) HVARKYEGYGLDIQDLVQDGCSGLMKTFEKFNPSKGCRFPTYAYWWIRQS
ZmSig6 (315) HVARKYEGCGLDIQDLVQDGCCGLMKTFEKFNPSKGCRFPTYAYWWIRQS
Consensus (351) HVARkYeg GLdiQDLVQDGc GLMKT EKFnPskGCRF TYAYWWIRQS
401 450
AtSig6 (371) IRKSIFQNSRTIRLPENVYMLLGKVSEARKTCVQEGNYRPSKEELAGHVG
OsSig6 (397) IKKSIFKNSRLIRLPESVYALLRKVGKARMECIMEGEQ-PTNANVARRAG
ZmSig6 (365) IKKSIFKNSRLIRLPESVYALLRKVGKARMECIMDGEQ-PTNEVVARRAG
Consensus (401) IKKSIFkNSR IRLPEsVY LL KVgkAR CI EGe -PTn VA raG
451 500
AtSig6 (421) VSTEKLDKLLYNTRTPLSMQQPIWSDQDTTFQEITPDSGIETPTMSVGKQ
OsSig6 (446) ITIEKLAKLRAKTRKPRSMQDHVWSDEGVTFQEITEDPNVEPPDLSVDRM
ZmSig6 (414) ITIEKLAKLRAKTRKVRSMQDRVWSDDGVTYQEITEDPNIEGPEVSVDRL
Consensus (451) IT EKL KL kTRk SMQd VWSDdgvTFQEIT DpnIE P LSVdR
501 550
AtSig6 (471) LMRNHVRNLLN-VLSPKERRIIKLRFGIDGGKQRSLSEIGEIYGLSKERV
OsSig6 (496) MMRQQVRDFLG-ILSPREKEIIEHRFGIHDGEPKTLHVIGDMFGLSKERI
ZmSig6 (464) MMRQQVRSFLTGILSPREKEIIEHRFGIHDGQPKTLHVIGDMYGLSKERI
Consensus (501) MMRQqVR fL -ILSPREKeIIe RFGIhdG KTL IGDMYGLSKERI
551 578
AtSig6 (520) RQLESRALYRLKQNMNSHGLHAYADLLV
OsSig6 (545) RQLQNRALEKLKRSASLQGFDVYFDLLT
ZmSig6 (514) RQVQNKALDKLRNSISAQGFHVYFDLLT
Consensus (551) RQLqnRAL KLKnsisaqGfhvY DLLt