Molecular characterization of a novel capillovirus from red currant

Karel Petrzik* ∙ Jaroslava Přibylová ∙ Igor Koloniuk ∙ Josef Špak

Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of the Czech Academy of Sciences, v.v.i., Branišovská 31, 370 05 České Budějovice, Czech Republic

Supplementary Fig.1.Phylogenetic NJ tree based on MP aa sequence

Unrooted phylogenetic tree of the MP aa sequences of Capilloviruses and related genera. Neighbor-Joining tree (JTT substitution model) with 1000 bootstrap replicates was produced in MEGA6.

Supplementary Table 1.Pairwise aa sequence comparison of RdRp polyprotein

Divavirus / Capillovirus
DiVB / DiVA / HarVA / CVA / CuVA / ASGV
DiVB / 58.5 / 53.3 / 22.7 / 20.7 / 22.4
DiVA / 55.7 / 21.9 / 21.2 / 21.4
HarVA / 21.9 / 21.6 / 22.3
CVA / 50.8 / 20.6
CuVA / 22.2
ASGV

Pairwise identity (%) based on CLUSTALW alignment, BLOSUM cost matrix and gap open cost 10.

Pairwise aa sequence comparison of MP

Chordovirus / Divavirus / Capillovirus / Citrivirus / Prunevirus / Capilloviru / Tepovirus
CChV2 / CChV1 / DiVA / DiVB / HarVA / CVA / CuVA / CLBV / AVCaV / CPrV / ASGV / PVT / PrVT
AHA85532 / AHA85535 / AFV57239 / AFV57241 / AEB00588 / NC_003689 / CuVA / CAC39423 / CDF66417 / AKN08995 / KF434636 / ACF39933 / AHM92767
CCHV2 / 63.3 / 17.7 / 17.2 / 19.0 / 14.4 / 14.7 / 14.4 / 15.2 / 13.5 / 15.4 / 19.5 / 15.1
CChV1 / 18.4 / 16.6 / 18.9 / 14.6 / 13.7 / 14.2 / 14.2 / 13.2 / 16.6 / 19.7 / 14.5
DiVA / 52.3 / 55.8 / 17.4 / 16.0 / 12.5 / 11.8 / 13.0 / 14.9 / 14.1 / 12.9
DivB / 47.4 / 13.7 / 13.3 / 11.5 / 13.0 / 11.0 / 13.6 / 12.4 / 11.9
HarVA / 17.4 / 17.9 / 13.0 / 15.0 / 13.4 / 16.5 / 15.4 / 13.4
CVA / 57.1 / 9.9 / 9.2 / 10.3 / 15.8 / 12.1 / 10.2
CuVA / 10.4 / 10.0 / 12.3 / 15.6 / 11.9 / 12.8
CLBV / 45.8 / 42.1 / 11.2 / 10.4 / 12.4
AVCaV / 35.3 / 12.3 / 13.4 / 12.4
CPrV / 9.8 / 11.9 / 12.7
ASGV / 15.4 / 12.7
PVT / 18.2
PrVT

Pairwise identity (%) based on CLUSTALW alignment, BLOSUM cost matrix and gap open cost 10.