NAME : ______

DNA WORKSHOP

1.  These websites will provide a detailed look at the structure of DNA.

First Website :

http://www.ucmp.berkeley.edu/glossary/gloss3/dna.html

Second Website :

http://www.accessexcellence.org/AB/GG/dna_molecule.html

Part 2 : Go to Third Website. This website will allow you to use Gel Electrophoresis to determine the sequence of some DNA, and help you solve a crime through DNA-Fingerprinting.

Third Website:

http://www.pbs.org/wgbh/nova/sheppard/analyze.html

Read intro page

Click On "NOVA image"

A)  Read "The Crime" then select "next" etc.

Follow lab procedures # 1-8

If it says “Start Over” you need to go back to

Step #1, and be more careful ! !

When doing Step #8 – put film into slot on developer.

Part 3 : Go to Fourth Website listed below. This website will allow you to take control of a strand of DNA. You will replicate (copy) the DNA just as your cells do, and then you will see how DNA can be used to make proteins/enzymes through (transcription and translation).

Fourth Website:

http://www.pbs.org/wgbh/aso/tryit/dna/#

B)  Read Intro Page

C)  Click on Go To DNA Workshop Activity

D)  Read Information

E)  Click on DNA Replication – Complete Replication

F)  Click on Protein Synthesis – Complete Transcription and Translation.

Name : ______Date : ______

DNA Workshop : Worksheet

BASIC BACKGROUND – From reading and notes

1. DNA is a “macromolecule” or polymer, made up of many repeating monomers. Each monomer or building block is called a nucleotide. What three basic units make up a single nucleotide?

______

2. All nucleotides are the same except for the type of base they attach to. There are four different nitrogenous (DNA) bases; name the four different nitrogenous bases:

a) ______c) ______
b) ______d) ______
3. Which bases have double rings? ______
4. What name is collectively given to any base that has two rings? ______
5. Bases complimentary pair up with other bases, but a given base can only bond up with its perfect match. Give the name of the bases that always match the base given below.

a) Adenine : ______b) Cytosine : ______
6. What type of bond forms between two complimentary bases? ______
First Website :

1. What is the proper name of the spiral shape that DNA takes? ______
2. If you took a typical piece of DNA from a Eukaryotic Cell (animal, plant etc.) and it you built a model of it to the same scale as the image on the screen, how far would it stretch? ______

3. Give the correct term for the procedure where DNA copies itself. ______
Second Website :

1. How many H- Bonds form between : A – T : ______C – G : ______
* Click on the “Structure Of Molecule” to see the structure of DNA.

2. What is the size of the distance between turns of the Helix?
* Hint: there are a thousand nanometers (nm) in a single micrometer (um), and a thousand (um) in one mm.

______nm à ______um à ______mm

OVER à

Part 2 : Gel Electrophoresis:

1. What do restriction enzymes do? ______
______
2. What size of DNA fragments tends to move most easily through the agarose?
______
3. What type of charge do the fragments of DNA have? ______
4. Why do scientists cover the gel with a nylon membrane? ______
______
5. What special property of the probes allows scientists to detect where the DNA fragments are located?
______
6. Who committed this crime? ______
Part 3 : Replication/Transcription/Translation

1. Give one example of a cell using DNA to make a proteins or new cells.

______
2. Where in the cell do replication and transcription take place? ______
3. Where in the cell does translation take place? ______
4. After translation has taken place, list the names of the three amino acids that this strand of DNA coded for. (In their correct order)

______