Eletronic Supplemntary Material
Table 1. Loci selected for all species, motif, sequence, species which was developed for, annealing temperature and number of alleles and observed and expected heterozigosities.
P. dominicana / S. similis / C. brissonii / S. frontalisLocus / Motif / Primer 5’-3’ / Developed for / AT/NA / Ho/He / AT/NA / Ho/He / AT/NA / Ho/He / AT/NA / Ho/He
SF01 / Di / F:TGACTCTGTCAGGTCTGTCT
R:ATGCAGCAAAGTCCATACCC / S. frontalis / 61C/2 / 0.533/0.404 / 61C/2 / 0.200/0.442 / - / - / - / -
CB01 / Di / F:GCATCTATTACACTCAGTAAACCCAGC
R:AGGGCTGTGCCTGTGTTTCTTTCT / C. brissonii / 67C/8 / 0.866/0.862 / - / - / - / - / 66C/20 / 0.880/0.936
CB02 / Tetra / F:ACCAGTAGAGTGCTTTGCCA
R:TTGGGTTTCCCAAGCTTTCCCA / C. brissonii / 66C/9 / 0.733/0.816 / 67C/11 / 1.000/0.889 / 67C/15 / 0.893/0.899 / - / -
PD01 / Tetra / F:ACAGAATGATGCATTAGAT
R:TTCTGAAGAGGACCACAG / P. dominicana / 59C/10 / 0.800/0.889 / - / - / - / - / - / -
PD02 / Tetra / F:ATGAAGTCCACCTGTCAATGCAGC
R:TGTGCTCTCAGACCCATATTACA / P. dominicana / 66C/9 / 0.933/0.896 / 61C/8 / 0.500/0.879* / 66C/15 / 0.876/0.883 / - / -
PD03 / Tetra / F:AGCTGTAATCCTCCTCCCACAACA
R:CAACAGCTCTTGCACTTGGA / P. dominicana / 64C/12 / 0.933/0.905 / 63C/13 / 0.800/0.931 / - / - / 63C/20 / 0.913/0.920
CB03 / Tetra / F:AAGAAGGACATGAGCGCACAGA
R:TGGTTCCTGTTGGCAATCCTGA / C. brissonii / 67/11 / 0.800/0.871 / 63C/10 / 1.000/0.868 / 67C/14 / 0.851/0.857 / - / -
SF02 / Di / F:CGCTGAAGGTGGGATTCATT
R:TTAGCAGCGACCAGAGTCTT / S. frontalis / 51/3 / 0.400/0.503 / - / - / - / - / - / -
CB04 / Tetra / F:TCAGCAACAGTATGAGGGCAGT
R:AGGCTTGTGCAGGTGTCATT / C. brissonii / - / - / 66C/9 / 0.800/0.895 / 67C/13 / 0.901/0.908 / - / -
CB05 / Tetra / F:GCAAAGGCACAATGAAGGAGTCCTGA
R:TCAGGCAGTTCTCTCCCAGTATGT / C. brissonii / - / - / 63C/8 / 0.700/0.879 / 67C/12 / 0.837/0.843 / - / -
CB06 / Di / F:AGCTCCTGTTCTCCACAGAT
R:TCGCACAGATTGAGGGACAGAT / C. brissonii / - / - / 63C/2 / 0.200/0.189 / - / - / - / -
SF03 / Di / F:CTGGGAAGGTCTTGGCATTT
R:GTGATGCTCACATCTTTCACAC / S. frontalis / - / - / 64C/9 / 1.000/0.868 / - / - / 63C/13 / 0.760/0.854
CB08 / Tetra / F:ATCCATCCATCCATGCATCC
R:TTGGCTCTGGTAGGTGATGAGGTT / C. brissonii / - / - / - / - / 66C/6 / 0.488/0.491 / - / -
CB09 / Hepta / F:TGTTGTGGAGACACCTTGGCTT
R:AAGCGGTTCACACCTTCCCAGAA / C. brissonii / - / - / - / - / 66C/9 / 0.790/0.795 / - / -
Table 2. Number of samples, number of loci tested with each methodology, number loci selected, mean number of alleles (Na), observed (Ho) and expected (He) heterozigosities for each species
Species / No of samples / No loci tested (MultiNa/Genotyping) / No loci selected / Mean Na / Mean Ho / HeP. dominicana / 15 / 56 / 19 / 8 / 8 (2-12) / 0.750 / 0.768
S. similis / 10 / 56 / 21 / 9 / 8 (2-13) / 0.688 / 0.760
C. brissonii / 70 / 56 / 18 / 7 / 12 (6-15) / 0.805 / 0.811
S. frontalis / 25 / 56 / 20 / 3 / 17 (13-20) / 0.851 / 0.903