3

SUPPORTING INFORMATION

Green synthesis of silver nanoparticle-decorated porous reduced graphene oxide for antibacterial non-enzymatic glucose sensors

Le Thuy Hoa, Ngo Thi Yen Linh, Jin Suk Chung and Seung Hyun Hur*

School of Chemical Engineering, University of Ulsan, Daehak-ro 93, Nam-gu, Ulsan 680-749, Korea

*Corresponding authors:

Fig. S1. The BET surface area of grGO at different GO to saccharide ratio fabricated with various saccharide species.

Fig. S2. The TEM image of silver NPS in Ag/grGO

Fig. S3. The Raman spectra of only GO and Ag/grGO.

Fig. S4. The relationships between the applied voltage and current (black line) and the applied voltage and S/N ratio (pink line) of the Ag/grGO_3 with the addition of 0.5mM glucose.

Table S1. The sensitivity of Ag/grGO at various Ag:GO ratios in each linear region.

Sample / Ag:GO
ratio / Sensitivity
(μA·mM−1·cm−2)
Linear range 1 / Correlation coefficient / Linear range 2 / Correlation coefficient

only grGO

/ - / 143.31 / 0.986 / 78.11 / 0.981

Ag/grGO_1

/

1:2

/ 320.18 / 0.991 / 129.036 / 0.987

Ag/grGO_2

/

2:3

/ 603.15 / 0.993 / 151.092 / 0.987

Ag/grGO_3

/

1:1

/ 731.03 / 0.994 / 163.06 / 0.991

Ag/grGO_4

/

3:2

/ 641.94 / 0.987 / 152.01 / 0.982

Ag/grGO_5

/

2:1

/ 372.62 / 0.989 / 118.495 / 0.984

Table S2. Comparison of different glucose sensors

Synthesis
process / Harmful agent / Glucose oxidation enzyme / Linear range / Limit of detection
(μM) / Sensitivity
(µA·mM-1·cm-2)
Our work / One step hydrothermal / x / x / 5Μm-3mM
3mM-8mM / 4 / 725.0
163.06
[a]1 / Boiling
and stirring / HNO3:HCl / horseradish peroxidase
glucose oxidase / 0.5–50μM / 0.1 / 1980.18
[b]2 / Two-step / x / glucose oxidase / 2–12mM / 4 / -
[c]3 / Evaporation / H2SO4
HNO3 / x / 125 μM–10 mM / 25 / 3.0856
[d]4 / Ball milling / x / horseradish peroxidase
glucose oxidase / 25 μM –
1.0 mM / 10 / 14
[e]5 / Electrochemical deposition / x / x / 1–14mM / 4 / 135

Table S3. The comparison of glucose concentration between Ag/grGO_3 and commercial ACCU-CHEK® Performa Blood Glucose Meter using HS and RS.

Dilution ratio of serums in DI water

/

Horse blood serum (mM)

/

Rabbit blood serum (mM)

Glucometer

/

Experiment

/

Glucometer

/

Experiment

pure

/

4.52

/

4.48

/

7.01

/

7.16

1:1

/

2.39

/

2.42

/

4.06

/

4.11

1:2

/

1.71

/

1.74

/

3.05

/

2.99

1:3

/

1.53

/

1.52

/

2.63

/

2.67

1:4

/

1.22

/

1.24

/

2.35

/

2.29

Percentage of difference (%)

/

1.34

/

1.83

Fig. S5. (a) Recovery, (b) stability and (c) sensitivity of Ag/grGO sensor with different saccharides.

REFRENCES

1.  Lin J, He C, Zhao Y, Zhang S (2009) Sens Actuators, B Chem 137 (2):768-773.

2.  Lu W, Luo Y, Chang G, Sun X (2011) Biosens Bioelectron 26 (12):4791-4797.

3.  Liu Y, Liu S, Chen R, Zhan W, Ni H, Liang F (2015) Electroanal 27 (5):1138-1143.

4.  Chen L, Xie H, Li J (2012) J Solid State Electrochem 16 (10):3323-3329.

5.  Joshi AC, Markad GB, Haram SK (2015) Electrochim Acta 161:108-114.