From,
Dr. RAJASHRI L WATHARKAR,
1st MD Dept of Rasashastra,
A.V.S., P.G.C.R.C., Ayurveda,
Mahavidyalaya, BIJAPUR.
To,
Registrar,
Rajiv Gandhi University of Health Sciences,
Bangalore.
Through,
The Principal & H.O.D
Dept of Rasashastra,
A.V.S., P.G.C.R.C., Ayurveda
Mahavidyalaya, BIJAPUR.
Respected Sir,
Sub: Submission of completed proforma for registration of subject for dissertation.
I humbly request you to kindly register the below subject against my name for submission of dissertation to Rajiv Gandhi University of Health Sciences, Bangalore for the partial fulfillment of M.D. (Ayu).
Title of dissertation :-
“Evaluation of spermatogenic activity of Panchamrita parpati in albino rats”
Here I am enclosing completed proforma for registration of subject for dissertation.
Thanking you,
Yours faithfully
Date :
Place : Bijapur (Dr. RAJASHRI L WATHARKAR)
Rajiv Gandhi University of Health Sciences, Karnataka
Bangalore
ANNEXURE II
PROFORMA FOR REGISTRATION OF SUBJECTS FOR DISSERTATION
1. Name of the candidate : Dr. RAJASHRI L WATHARKAR
and Address : C/O M.K.WATHARKAR,
(in block letters) AAMIR TALKIES ROAD
IN FRONT OF K.E.B OFFICE
BIJAPUR - 586101
2. Name of the Institution : AVS’S POST GRADUATE CUM RESEARCH CENTRE, AYURVEDA MAHAVIDYALAYA, VIDYA NAGAR, BAGALKOT ROAD, BIJAPUR - 586101
3. Course of study and subject : M.D (Ayu) RASASHASTRA
4. Date of admission to course : 23 – 05 - 2010
5. Title of Topic : EVALUATION OF SPERMATOGENIC
ACTIVITY OF PANCHAMRITA PARPATI
IN ALBINO RATS.
6. Brief resume of the intended work:
6.1 Need for the study
Reproduction is unique property and vital process of the living being. This can produce young ones like themselves after having attained maturity. Thus continuity of life can be maintained.1 In ancient time it was believed that without having child one cannot get Moksha. Man having numerous progeny is like one having numerous forms, faces, troops, action, eyes, knowledge and selves. He is praised as auspicious commendable, worthy, potent and numerously branched. Such belief made the man to get more number of children as well as man’s great reproductive potential.2
Drastic changes of life style, diet, work related stress etc in the modern era have negatively affected physical and mental health, including incidence of infertility and impotency. In the 2001 W.H.O respect of a meeting on current practices and controversies in Assisted reproduction, it is mentioned that infertility affect more than 80 million people worldwide.3 According to World Health organization, 51.2% couples are infertile because of a male factor.4 W.H.O also recommended that infertility should be recognized as a public health issue worldwide, including developing countries.5
In India primary and secondary infertility figures, as given in WHO studies, are 3% and 8%. The Ninth Five Year plan (1997-2002) included infertility in the comprehensive reproductive and child health package.6
Based on 2005 NSFG (National survey Family Growth) U.S.A, approximately 12% of American couples experienced impaired Fecundity in 2002. This is 20% increase from 6.1 million couple who reported an inability to have children in 19957.
Rasashastra medicine are considered as having quick action and better effect than herbal medicine with minimum dose and without unpleasant taste. Most of Rasashastra texts have mentioned Panchamrita parpati having vrushya property. Hence the present study under taken to assure the quality and provide scientific data for its vrushya property by evaluation of the spermatogenic activity.
6.2 Review of literature
The detailed description of Panchamrita parpati explained in Bhaishajya Rathavali8, Rasendrasara sangraha9, Rasa kaamdhenu10, Rasendra chintamani11 for Grahani roga, Jwara, Vrishya etc.
1) The method of preparation of Panchamrita parpati according to Bhaishajya Ratnavali8.
2) Spermatogenic activity will be evaluated according to the method of estimation of concentration of spermatozoa in the cauda epididymis, laboratory Animal12.
Previous study conducted :
1) Management of chronic colitis by Panchamrita parpati in 1981.13
2) Certain immunological study in case of chronic cholitis under influence of
Panchamrita parpati in 1982.14
3) Concept of grahani and evaluation of the role of fortified Panchamrita parpati and Mustakarishta in it’s management in 1984.15
4) Role of Panchamrita parpati and Chitrakadi vati in grahani roga. A comparative
clinical and experimental study in 1987.16
5) Clinical evaluation of Panchamrita parpati in grahani roga w.s.r it’s dosage in
1996.17
6) Chemical analysis of Ashtavidh samskarita parad and it’s spermatogenic effect
in albino rats in 2000.18
7) Analytical study of shodhita harital and harital bhasma and assessment of their
Spermatogenic activity on albino rats in 2004.19
8)Physico-chemical analysis and evaluation of spermatogenic activity of Swarnamakshika bhasma in albino rats in 2010.20
6.3 Objective of study.
1) To evaluate the spermatogenic activity of Panchamrita parpati.
2) To provide an effective economic and safe medicine for spermatogenic activity.
7. Material and methods.
7.1 Source of the data.
Materials
1. Materials for preparation of Panchamrita parpati.
a) Material for preparation of Panchamrita parpati according to Bhaishajya Ratnavali
8th chapter, Page : 168, Shloka no: 458 – 460.
b) Ingredients required shuddha Parada, shuddha Gandhaka, Loha bhasma, Abhraka bhasma, Tamra bhasma and equipments will be collected from the practical pharmacy of A.V.S’s Ayurveda Mahavidyalaya Bijapur
2. Material for spermatogenic activity study
a) Panchamrita parpati
b) 24 male albino rats (wister strain) animal will be taken from A.V.S’S Ayurveda
Mahavidyalaya Postgraduate cum Research Centre’s animal house.
c) Ghee.
7.2 Method of collection of data ( including sampling procedure if any)
A. 1 Method of preparation of panchamrta parpati will be followed according to Bhajshajya Ratnavali 8th chapter, Page no. : 168, Shloka no: 458 – 460.
B. Method of Spermatogenic activity study
1. 24 Wister strain albino rats will be collected from A.V.S’S PGCRC animal house
by considering
Inclusive criteria
1. Healthy male albino rats of 90 – 100 days old.
2. Weight ranges 175 -200 gms.
Exclusive criteria
1. Unhealthy male albino rats
.
2. Female albino rats.
3. Weight range below 175 gms and above 200gms.
4. Below 90 days old and above 100 days old.
Drug Schedule for experimental study
Group I : Test drug. [Minimum dose]
Drug : Panchamrita parpati with ghee.
Dose : 4.5 mg/200gm body weight
Dosage Form : Liquid
Route : Oral
Group II : Test drug. [Moderate dose]
Drug : Panchamrita parpati with ghee.
Dose : 13.5 mg/200 gm body weight
Dosage Form : Liquid
Route : Oral
Group III : Test drug. [Higher dose]
Drug : Panchamrita parpati with ghee.
Dose : 18 mg/200 gm body weight
Dosage Form : Liquid
Route : Oral
Group IV : Control
Drug : Ghee
Dose : ½ ml/200 gm body weight
Dosage Form : Liquid
Route : Oral
Animal Experimental data
For selection of albino rats statistical techniques will not be applied all the rats will be examined in details considering inclusive and exclusive criteria. The data will be collected by detailed observation of albino rats and investigation.
INVESTIGATIONS
Before administration of drug / After administration of drugBody weight
Gravimetric and Biochemical analysis / After administration of drugs
G-I / G-II / G-III / G-IV
1. Cholestrol ( mg/gm)
2. Glycogen (mg/gm)
3. Protein (mg/gm)
Effect of drugs on accessory organ in albino rats (organ wt mg/100gm body weight) / After administration of drugs
G-I / G-II / G-III / G-IV
1. Testis
2. Epididymis
3. Seminal vesicle
4. Prostate
5. Vas deferens
Macrometric changes of testis / After administration of drugs
G-I / G-II / G-III / G-IV
1. Diameter of testis ( mm)
2. Diameter of tubules (mm)
Effect of drugs on Spermatogenic element in albino rats / After administration of drugs
G-I / G-II / G-III / G-IV
1. Spermatogonia
2. Spermatocytes
3. Spermatids
4. Sperm count (million/cauda)
STUDY GROUP
1. Sample size
a) 24 male albino rats will be taken for experimental study.
b) 6 albino rats will in each group.
Group-I : Administration of Panchamrita parpati with ghee (Minimum dose)
Group-II : Administration of Panchamrita parpati with ghee (Moderate dose)
Group-III : Administration of Panchamrita parpati with ghee (Higher dose)
Group-IV : Administration of ghee.
Statistical analysis
Will be done by using statistical significance test ie ‘t’ test (paired and unpaired) mean ± SD diagrammatic presentation.
7.3 Does the study require any investigation to be conducted on patients or other humans or animals? If so, please describe briefly
The study require investigation to be conducted on albino rats are as follows
a) Gravimetric and Biochemical
b) Micrometric.
c) Spermatogenic element.
d) Accessory sex organ weight.
7.4 Has ethical clearance been obtained from your institution in case of 7.3
Yes, ethical committee has recommended the project.
8. List of references
1 / Ganguli, Das, Datta / : / Chapter XLVTII, College of Botany, New Central group agency private Ltd. Chintamani Das Lane, Calcutta, 1st Edn, Volume 1, Page – 7112 / Shri Pandit Kashinath Shatri / : / Chapter -2 Charaka Samhita Chikistasthana Vajeekarana Chikista chaukamba sanskrita
Bhavana Varanasi 4th Edition – 1976, Page – 68, 69.
3 / www.who.int/reproductive-health/infertility / : / Current Practices and Controversies in Assisted Reproduction, World Health Organization, Geneva, 2002 ; Page : XV
4 / Herman Tournaye www.who.int/reproductive- health/infertility / : / Current Practices and Controversies in Assisted Reproduction, World Health Organization, Geneva, 2002; Gamete source and manipulation, Page : 83
5 / www.who.int/reproductive-health/infertility / : / Current Practices and Controversies in Assisted Reproduction, W.H.O, Geneva, 2002; Page : 382
6 / Anjali Widge
www.who.int/reproductive -health/infertility / : / Current Practices and Controversies in Assisted Reproduction, World Health Organization, Geneva, 2002; Sociocultural attitudes towards infertility & assisted reproduction in India; Page 60
7 / Julia R.Barret www.ehponline.org/members/2006/114-11 / : / Environmental Health Perspectives Volume-114, No.:11, Fertile grounds for enquiry :environmental effects on human reproduction; Nov 6,2006
8 / Kaviraj shri Ambika data Shastri Ayurvedacharya / : / Bhaishjya Ratnavali, Pubn Choukhamba Sanskrit Sansthan 1991, 8th Chapter, Page No:168
9 / Shri Gopal Krishna Bhatta / : / Rasendrasarasangraha Pub: Choukhamba sanskrit Sansthana,Varanasi, 1stEdn-2007, P. No: 314 - 315
10 / Acarya Shri Gulraj Sharma Misra & Vaidya Santosh Kumar Sharma / : / Rasa Kaam Dhenu Chapter - Grahani Nidana, Publication – Choukhamba Orientalia, Varanasi, 2nd Edition, 1999, Page No: 257 – 258.
11 / Prof Siddninandan Mishra / : / Rasendra chintamani, 9th Chapter, Grahani rogadhikar Publication – Chaukhamba Orientalia Varanasi. Page No: 198-199
12 / KempinasW.G Lamano-Carvasho T.I / : / A method for estimation of the concentration of spermatozoa in the cauda epididymis, Laboratory animals; 1987; Page: 154-156.
13 / Dr. Vinod Kumar / : / Management of Chronic Colitis by Panchamrita parpati, Dissertation Banaras Hindu University, Varanasi, 1981, Dept of Shalyashalakya, Varanasi.
14 / Sachan. A.K / : / Certain Immunologics study in case of chronic cholitis under influence of panchamrita parpati, Dissertation Banaras Hindu University, Varanasi, 1982, Dept of Shalyashalakya, Varanasi.
15 / Tewari C.M / : / Concept of Krimija Grahani and Evaluation of the role of fortified Panchamrita Parpati and Mustakarishta in it’s Management, Dissertation Banaras Hindu University, Varanasi, 1984, Dept of Kayachikitsa, Varanasi.
16 / Mishra N.D / : / Role of Panchamrita parpati Chitrakadivati in Grahaniroga - A Comparative & experimental study, Ph.D Dissertation Lucknow University 1987, Dept of Kayachikitsa, State Ayurvedic College Lucknow.
17 / Reema K.V / : / Clinical evaluation of Panchamrita Parapati in Grahani roga w.s.r it’s Dosage, Dissertation Kerala University, Trivandrum, 1996, Dept of Rasashastra, Govt. Ayurveda College Trivandrum.
18 / Dr.R.S.Sarashetti & Biradar Vilas O / : / Chemical analysis of Ashtavidha samskritha parad and its spermatogenic effect in albino rats, Dissertation, RGUHS, Karnataka; 2000; Dept. of Rasashastra, PGCRC, A.V.S’s Ayurveda Mahavidhyalaya, Bijapur.
19 / Dr.R.S.Sarashetti & Shashidhar J. / : / Analytical study of Shoditha harithala and harithala bhasma and assessment of their spermatogenic activity on albino rats, Dissertation, RGUHS, Karnataka; 2004; Dept. of Rasashastra, PGCRC, A.V.S’sAyurveda Mahavidhyalaya, Bijapur.
20 / Dr.R.S.Sarashetti & Vinod.C / : / Physico-chemical analysis and evaluation of spermatogenic activity of Swarnamakshika bhasma in albino rats, Dissertation, RGUHS, Karnataka; 2010; Dept. of Rasashastra, PGCRC, A.V.S’sAyurveda Mahavidhyalaya, Bijapur.
9. Signature of the candidate :
10. Remarks of the guide :
11. Name and Designation of
11.1 Guide :
11.2 Signature :
11.3 Co-Guide :
11.4 Signature :
11.5 Head of Department : Dr.R.S.Sarashetti M.D (Ayu), Ph.D, F.I.C.A.
Professor / H.O.D
A.V.S,P.G.C.R.C., Ayurveda Mahavidyalaya, BIJAPUR.
11.6 Signature :
12
12.1 Remarks of the
Chairman & Principal :
12.2 Signature :
A.V. Samiti’s
POST GRADUATE CUM RESEARCH CENTER
Ayurveda Mahavidyalaya, Bijapur-586101.
ETHICAL COMMITTEE
A.V. Samiti’s
Post Graduate Cum Research Centre,
Ayurveda Mahavidyalaya,
Bijapur-586101.
ETHICAL CLEARANCE CERTIFICATE
The Ethical Committee of this college had met on 25/10/2010 at 11:00 a.m. to scrutinize the synopsis of various post graduate students of the college from Ethical Clearance point of view.
The synopsis of thesis titled “EVALUATION OF SPERMATOGENIC ACTIVITY OF PANCHAMRITHA PARPATI IN ALBINO RATS”. of Dr. Rajashri L Watharkar., P.G. in Rasashastra, Department of Rasashastra of this college has been accorded Ethical Clearance by the E.C. in its meeting on 25/10/2010.
Chairperson
Ethical Committee
Date :
Note :