Appendices (Supplementary data)
A1: The HPLC (RI)-ESI/MS and corresponding fragmentation masses (m/z values) of compound of the mesocarp saponins (ME) of the Balanites aegyptiaca fruit in the negative ion mode (Peaks 1- 8 of Figure 3A)
Peak / Rt(min)a / (M-H)-b / Fragmented ionsc
1 / 4.2 / 1195 / 1063 [M-H-Xyl], 1049 [M-H-Rha], 901[M-H-Glu-Xyl], 755 [M-H-Glu-Rha-Xyl], 593 [M-H-2Glu-Rha-Xyl], 431[M-H-3Glu-Rha-Xyl]
2 / 4.8 / 1063 / 901 [M-H-Glu], 755 [M-H-Glu-Rha], 593 [M-H-2Glu-Rha], 431[M-H-3Glu-Rha]
3 / 6.0 / 1077 / 915 [M-H-Glu], 769[M-H-Glu-Rha], 589[M-H-2Glu-Rha-H2O],
431[M-H-3Glu-Rha-Me]
4 / 7.2 / 1209 / 1077 [M-H-Xyl], 897[Glu-Xyl-H2O], 589[M-H-2Glu-Rha-Xyl-H2O],
431[M-H-3Glu-Rha-Xyl-Me]
5* / 8.2 / 1077 / 931[M-H-Rha], 915[M-H-Glu], 897[M-H-Glu-H2O], 769[M-H-Glu-Rha], 589[M-H-2Glu-Rha-H2O], 431[M-H-3Glu-Rha-H2O]
6* / 9.6 / 1209 / 1195 [M-H-14], 1077[M-H-Xyl], 915[M-H- Glu-Xyl], 769[M-H-Glu-Rha-Xyl],
589[M-H-2Glu-Rha-Xyl-H2O], 431[M-H-3Glu-Rha-Xyl-H2O]
7* / 11.1 / 1077 / 931[M-H-Rha], 915[M-H-Glu], 897[M-H-Glu-H2O], 769[M-H-Glu-Rha], 589[M-H-2Glu-Rha-H2O], 431[M-H-3Glu-Rha-H2O]
8 / 10.9 / 1045 / 899[M-H-Rha], 883 [M-H-Glu], 737[M-H-Glu-Rha], 593[M-H-Glu-Rha-(Rha-2H)], 431[M-H-2Glu-Rha-(Rha-2H)]
a Retention time, b Deportonated molecular ion, c Fragmented ions
Glu-glucose, Rha-rhamnose, Xyl-xylose, Me-methyl
*Isomerm/z values quoted are integers obtained by truncating measured values.
A 2: The HPLC (RI)-ESI/MS and corresponding fragmentation masses (m/z values)of compound of the kernel cake saponins (KE) of the Balanites aegyptiaca fruit in the negative ion mode (Peaks 1- 9 of figure 4 A)
Peak / Rt(min)a / (M-H)-b / Fragmented ionsc
1 / 5.4 / 1195 / 1063 [M-H-Xyl], 1049 [M-H-Rha], 901[M-H-Glu-Xyl], 755 [M-H-Glu-Rha-Xyl], 593 [M-H-2Glu-Rha-Xyl], 431[M-H-3Glu-Rha-Xyl]
2 / 6.1 / 1063 / 901 [M-H-Glu], 755 [M-H-Glu-Rha], 593 [M-H-2Glu-Rha], 431[M-H-3Glu-Rha]
3 / 7.5 / 1209 / 1077 [M-H-Xyl], 897[Glu-Xyl-H2O], 589[M-H-2Glu-Rha-Xyl-H2O],
431[M-H-3Glu-Rha-Xyl-Me]
4 / 9.5 / 1223 / 1077 [M-H-Rha], 1061[M-H-Glu], 915[M-H-Glu-Rha],769[M-H-Glu-2Rha], 589[M-H-2Glu-2Rha-H2O], 431[M-H-3Glu-2Rha-Me],
5 / 10.9 / 1077 / 915 [M-H-Glu], 769[M-H-Glu-Rha], 589[M-H-2Glu-Rha-H2O],
431[M-H-3Glu-Rha-Me]
6* / 12.6 / 1209 / 1195 [M-H-14], 1077[M-H-Xyl], 915[M-H- Glu-Xyl], 769[M-H-Glu-Rha-Xyl],
589[M-H-2Glu-Rha-Xyl-H2O], 431[M-H-3Glu-Rha-Xyl-H2O]
7* / 14.6 / 1077 / 931[M-H-Rha], 915[M-H-Glu], 897[M-H-Glu-H2O], 769[M-H-Glu-Rha], 589[M-H-2Glu-Rha-H2O], 431[M-H-3Glu-Rha-H2O]
8 / 16.4 / 1045 / 899[M-H-Rha], 883 [M-H-Glu], 737[M-H-Glu-Rha], 593[M-H-Glu-Rha-(Rha-2H)], 431[M-H-2Glu-Rha-(Rha-2H)]
9* / 23.3 / 1045 / 899 [M-H-Rha], 883[M-H-Glu], 737[M-H-Glu-Rha], 593[M-H-Glu-Rha--(Rha-2H)], 431[M-H-2Glu-Rha-(Rha-2H)]
a Retention time, b Deportonated molecular ion, c Fragmented ions
Glu-glucose, Rha-rhamnose, Xyl-xylose, Me-methyl
*Isomerm/z values quoted are integers obtained by truncating measured values.
A 3: The HPLC (RI)-ESI/MS and corresponding fragmentation masses (m/z values) of compound of the kernel cake saponins (KE) of the Balanites aegyptiaca root in the negative ion mode (Peaks 1- 9 of figure 5 A).
Peak / Rt(min)a / (M-H)-b / Fragmented ionsc
1 / 5.5 / 1195 / 1063 [M-H-Xyl], 901[M-H-Glu-Xyl], 755 [M-H-Glu-Rha-Xyl], 593 [M-H-2Glu-Rha-Xyl], 431[M-H-3Glu-Rha-Xyl],
2 / 6.2 / 1063 / 901 [M-H-Glu], 755 [M-H-Glu-Rha], 593 [M-H-2Glu-Rha], 431[M-H-3Glu-Rha], 413[M-H-3Glu-Rha-H2O]
3 / 7.6 / 1209 / 1077 [M-H-Xyl], 1063 [M-H-Rha], 1045 [M-H-Xyl-H2O-CH3], 915 [M-H-Xyl-Glu], 901[M-H-Rha-Glu], 883[M-H-Glu-Rha-H2O], 769[M-H-Glu-Rha-Xyl], 755[M-H-Glu-Rha-Xyl-14], 593[M-H-2Glu-Rha-Xyl-Me],
431[M-H-3Glu-Rha-Xyl-Me]
4 / 9.5 / 1223 / 1077 [M-H-Rha], 1061[M-H-Glu], 915[M-H-Glu-Rha],769[M-H-Glu-2Rha], 589[M-H-2Glu-2Rha-H2O], 431[M-H-3Glu-2Rha-Me], 413[M-H-3Glu-2Rha-Me-H2O]
5 / 9.9 / 1529 / 1385 [M-H-(Rha-2H)], 1223[M-H-(Rha-2H)-Glu], 1077[M-H-(Rha-2H)-Glu-Rha], 1045[M-H-(Rha-2H)-Glu-Rha-H2O-Me], 901[M-H-(Rha-2H)-2Glu-Rha-Me], 755[M-H- (Rha-2H)-2Glu-2Rha-Me], 593[M-H- (Rha-2H)-3Glu-2Rha-Me], 431[M-H-(Rha-2H)-4Glu-2Rha-Me-H2O]
6 / 10.7 / 1585 / 1571[M-H-Me], 1427[M-H-Me-(Rha-2H)], 1385[M-H-Me-(Rha-2H)-42], 1223[M-H-Me-(Rha-2H)-42-Glu], 1077[M-H-Me-(Rha-2H)-42-Glu-Rha], 915[M-H-Me-(Rha-2H)-42-2Glu-Rha], 901[M-H-2Me-(Rha-2H)-42-2Glu-Rha], 755[M-H-2Me-(Rha-2H)-42-2Glu-2Rha], 593 [M-H-2Me-(Rha-2H)-42-3Glu-2Rha], 431 [M-H-2Me-(Rha-2H)-42-4Glu-2Rha]
7 / 11.6 / 1571 / 1427[M-H-(Rha-2H)], 1385[M-H-(Rha-2H)-42], 1223[M-H-(Rha-2H)-42-Glu], 1077[M-H-(Rha-2H)-42-Glu-Rha], 915[M-H-(Rha-2H)-42-2Glu-Rha], 901[M-H-Me-(Rha-2H)-42-2Glu-Rha], 755[M-H-Me-(Rha-2H)-42-2Glu-2Rha], 593 [M-H-Me-(Rha-2H)-42-3Glu-2Rha], 431 [M-H-Me-(Rha-2H)-42-4Glu-2Rha]
8* / 12.5 / 1223 / 1077 [M-H-Rha], 1061[M-H-Glu], 915[M-H-Glu-Rha],769[M-H-Glu-2Rha], 589[M-H-2Glu-2Rha-H2O], 431[M-H-3Glu-2Rha-Me], 413[M-H-3Glu-2Rha-Me-H2O]
9 / 13.6 / 1515 / 1371[M-H-(Rha-2H)], 1209[M-H-(Rha-2H)-Glu], 1063[M-H-(Rha-2H) -Glu-Rha], 901[M-H-(Rha-2H) -2Glu-Rha], 755[M-H-(Rha-2H) -2Glu-2Rha], 593[M-H-(Rha-2H) -3Glu-2Rha], 431[M-H-4Glu-2Rha-(Rha-2H)]
10* / 14.5 / 1529 / 1385 [M-H-(Rha-2H)], 1223[M-H-(Rha-2H)-Glu], 1077[M-H-(Rha-2H)-Glu-Rha], 1045[M-H-(Rha-2H)-Glu-Rha-H2O-Me], 901[M-H-(Rha-2H)-2Glu-Rha-Me], 755[M-H- (Rha-2H)-2Glu-2Rha-Me], 593[M-H- (Rha-2H)-3Glu-2Rha-Me], 431[M-H-(Rha-2H)-4Glu-2Rha-Me-H2O]
aRetention time, bDeportonated molecular ion, cFragmented ions
Glu-glucose, Rha-rhamnose, Xyl-xylose, Me-methyl
*Isomerm/z values quoted are integers obtained by truncating measured values.
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