Appendix e-1

cDNA Primers

Gene / Primer / Primer sequence
COL6A1 / COL6A1N1 F / ACT CTG GCT GGGAGCAGA AG
N domain / COL6A1N1 R / TCG GTC ACC ACA ATC AGG TA
COL6A1N2F / CTACACCGA CTG CGC TAT CA
COL6A1N2R / GGCTTCTCCCTTCTCTCC TG
COL6A1 / COL6A11F / GCT TTG AGG GAG AAC GAG G
triple helix / COL6A11NR / TTC ACC AGG GTC TCCTCT TG
COL6A12F / CTG GAG AGA GAG GAC CAC G
COL6A12R / GGT CCT TGG TGT CCT GGG
COL6A1 / COL6A1C1F / GTC AAA GGA GCA AAG GGG TA
C terminal / COL6A1C1R / GAG CCT GGGATG AAG TCA AA
COL6A1C2F / ACT CAG AGG GAC ACC ACA CC
COL6A1C2R / GGT GGC GTC GTT GAT AAA GT
COL6A1C3F / CCA CAACTT TGA CACCAC CA
COL6A1C3R / GTT GTG TCC TGG CTTTCC AA
COL6A2 / COL6A2N1nF / CTCCTCGGG ACC AGG ACT
N domain / COL6A2N1R / CCCTGC AGG TTCTTGATG A
COL6A2N2F / TGC ACT TCT CTG ACC AGG TG
COL6A2N2R / GGT CTC CCT GTC TTC CCT TC
COL6A2 / COL6A21F / GCT ACC GTG GAC AGA AGG G
triple helix / COL6A21NR / TTT GTT GCC AACCTCTCC AG
COL6A22Fn / GGG TAT CAAGGCAAC AAT GG
COL6A22R / CTCGGGTCC TGG GAC TCC
COL6A2 / COL6A2C1F / TGG CTT GAA AGG AGA ACC TG
C terminal / COL6A2C1R / ACT TGA GGG CTG AGG GTG T
COL6A2C1v2F / TGA CCT ACG TGA GGGAGA CC
COL6A2C1v2R / TCACTC TCG TGCTTC TCG TG
COL6A2C2F / GGT GCA GTACAG CCA CGA G
COL6A2C2R / TTG GCA GGG AAG GTC TGG
COL6A2 / COL6A2C3F / GAC CTG GTC GCT GAG AAG TT
variant 2c2 / COL6A2C3a_2c2R / GTG GTG AGC ACG TCCATG T
COL6A2C3b_2c2F / AGCTGT CCT TCG TGTTCC TC
COL6A2C3b_2c2R / CAGGTA GGT CAG GAG CCT TG
COL6A2 / COL6A2C3F / GAC CTG GTC GCT GAG AAG TT
variant 2c2a / COL6A2C3_2c2aR / CACCAGCATGGG AAG AGG
COL6A2 / COL6A2C3F / GAC CTG GTC GCT GAG AAG TT
variant 2c2a' / COL6A2C3_2c2a'R / GTG AGC ACA GGG ACA GGA AG
COL6A3 / COL6A3N10T1aF / GGT TTGCTC AGG GGT TCA TA
N domain / COL6A3N10T1aR / TTA ACG CTCCTTCAT CTG CAT
COL6A3N9T1bF / TGC TGA TGT TAA CGT GTT TGC
COL6A3N9T1bR / CACCAA GTT GTC ATC GGT AGC
COL6A3N8T1cF / AAG CAG GCT AGC GTG TTC TC
COL6A3N8T1cR / AGC GAT CTCTTCCAGCTC AG
COL6A3N7T1dF / CCA GGA GCT GAA GAG AAG CA
COL6A3N7T1dR / GGCAGCTTGCAA ATA GGA GT
COL6A3N6T1eF / GCT CCT GCT TCT GCT CAC A
COL6A3N6T1eR / TTG GCT TGG AAG ATG AAA GG
COL6A3N5T1fF / GGT CAT CTG ACC GTG TGG AT
COL6A3N5T1fR / AAG TCCGGGATG AAG GAG AT
COL6A3N4T1gF / TCC GTG GTC GTG AAG AGG
COL6A3N4T1gR / ATG AGG ACC AGG AACTGT GG
COL6A3N3T1hF / CTG GAG TAC GTG TCC AGG AA
COL6A3N3T1hR / CAG GAC CAG GAC CAG GTG T
COL6A3N2T1iF / CTG AAC ACT GGC AAG GCT CT
COL6A3N2T1iR / CCT CAG GCA CAAAGT GGT TT
COL6A3N1aT1jF / TGG TCT ACA AAG GGG GAA GA
COL6A3N1aT1jR / GTC AAA GTC AAA GGCCTC CA
COL6A3N1bT1kF / CGCCATCTT GAA CAG AAT CA
COL6A3N1bT1kR / AGC TTT CTCGGC AAT GTT GT
COL6A3 / COL6A3N3T1hF / CTG GAG TAC GTG TCC AGG AA
N domain / COL6A3N3T2spR / CTC GCA CTG GAG GTG GAT AG
variant 2 specific / COL6A3N2T2spF / CTATCC ACC TCCAGTGCG AG
COL6A3N2T1iR / CCT CAG GCA CAAAGT GGT TT
COL6A3 / COL6A3N7T1dF / CCA GGA GCT GAA GAG AAG CA
N domain / COL6A3N7aT3spR / CTCTCTTCAGGG GTT CCA GAG
variant 3 specific / COL6A3N6bT3spF / CTC TGG AACCCC TGA AGA GAG
COL6A3N6T1eR / TTG GCT TGG AAG ATG AAA GG
COL6A3 / COL6A3N9T1bF / TGC TGA TGT TAA CGT GTT TGC
N domain / COL6A3N9T4aspR / TTGTTG ACT TCAATG ACT GTG ATG
variant 4 specific / COL6A3N8T4bspF / CATCACAGTCAT TGA AGTCAA
COL6A3N8T1cR / AGC GAT CTCTTCCAGCTC AG
COL6A3 / COL6A3N10T1aF / GGT TTGCTC AGG GGT TCA TA
N domain / COL6A3N10T5aspR / GAG TCTTGT GCT GCT TGC TG
variant 5 specific / COL6A3N9T5bspF / CAGCAA GCA GCA CAA GAC TC
COL6A3N9T1bR / CACCAA GTT GTC ATC GGT AGC
COL6A3 / COL6A31F / AACTTG GAG CGG CTAATG C
triple helix / COL6A31R / CTG TGC ACC TCT GGT CCC
COL6A32F / AAC CCA GGA CAA GAC AGC
COL6A32R / GCA CAT TGA TCG ATG GAG TC
COL6A3 / COL6A3C1F / CCT CCA ATA GTT GGA CA
C terminal / COL6A3C1R / TTT GAG CACAGCCTCTCT GA
COL6A3C2F / TGTGGCCAG GAA CAC ATT TA
COL6A3C2R / CAG GGA GAA TTCCACCTT CA
COL6A3C3F / CCT GGT CAG ACA ACT GGA CA
COL6A3C3R / GTC CCCTTG GAA CCA ATC AC
COL6A3C4F / TTC GTC AGCAGT GAA AAT GC
COL6A3C4R / GAC TGA TCA TGG GCT GAG GT
COL6A3C5F / ACT GGGAGAGGC CTG AGC
COL6A3C5R / CACCCG GAG CTTCTACAG AG

gDNA Primers

COL6A2exon17 Forward CTGCAGTGTCCACAGGATTG , Reverse CTGAGAACAGGAGGCACAGG; COL6A2exon23 Forward CAGGACCCGAGGTAGGTTG Reverse ACCAACCTTGCCCTGTGA; COL6A2exon2 Forward ATCCCGCTGACCTGCTGT Reverse CTGGGTACTGGTCCCAACTG; COL6A2exon11 Forward ACTCCCAGGTCAAACCACAG Reverse AGTTTGGGATGCATCAGGTC; COL6A2exon26 Forward CAACGAGGGCCTCTGCAT Reverse GGGCTCACGCTAGACAGC; COL6A1exon9 Forward GTGATGGGGAAGGTGCTTTA Reverse ATAGGATTTGGGCCAAGGAG; COL6A1exon14 Forward GGACAGTGTGAAGGTGACCA Reverse CCGCAAGTCACTCACCTTTT.

Appendix e-1. cDNA and gDNA primers used in the PCR analysis of the COL6A1-3 genes. Further information can be obtained by contacting