Supp. class Table1 Class level distribution of OTUs detected on PhyloChips

Sr. No. / Classes detected on PhyloChips / Edgemont site† / North Cave Hills site†
1.  / Acidobacteria / 30, + / 31, +
2.  / Acidobacteria-10 / 1 / 1
3.  / Acidobacteria-4 / 5, + / 5, +
4.  / Acidobacteria-5 / 1 / 1
5.  / Acidobacteria-6 / 12, + / 16, +
6.  / Actinobacteria / 187, + / 229, +
7.  / Alpha proteobacteria / 215, + / 280, +
8.  / Anaerobranca / - / 1
9.  / Anaerolineae / 8 / 13
10.  / Aquificae / 2 / 2
11.  / Bacilli / 83 / 124, +
12.  / Bacteroidetes / 23 / 26
13.  / BD2-10 group / 1 / 3
14.  / Beta proteobacteria / 127, + / 148, +
15.  / Catabacter / 3 / 6
16.  / CH21 cluster / 2 / 3
17.  / Chlamydiae / 1 / 1
18.  / Chlorobia / 3 / 2
19.  / Chloroflexi-3 / 1, + / 2
20.  / Chloroflexi-4 / 1 / 2
21.  / Clostridia / 127 / 139, +
22.  / Cyanobacteria / 18, + / 33
23.  / Dehalococcoidetes / 4 / 5
24.  / Delta proteobacteria / 78, + / 87, +
25.  / Desulfotomaculum / 4 / 4
26.  / Dictyoglomi / - / 1
27.  / Epsilon proteobacteria / 33 / 37
28.  / Flavobacteria / 27 / 30
29.  / Gamma proteobacteria / 151 / 227, +
30.  / gut clone group / 2 / 2
31.  / KSA1 / 1 / 1
32.  / mgA-1 / - / 1
33.  / mgA-2 / 1 / 1
34.  / Mollicutes / 5 / 8
35.  / NC10-1 / 2 / 1
36.  / NC10-2 / - / 1
37.  / Nitrospira / 1, + / 6, +
38.  / OP11-5 / 1 / 1
39.  / OP9 / 2 / 2
40.  / Planctomycetacia / 8 / 11
41.  / Solibacteres / 3 / 3, +
42.  / Sphingobacteria / 41, + / 54, +
43.  / Spirochaetes / 20 / 30
44.  / Symbiobacteria / 3 / 2
45.  / Thermodesulfobacteria / 1 / 1
46.  / Thermomicrobia / 2 / 2
47.  / Thermotogae / 1 / 1
48.  / TM7-3 / 1 / 3, +
49.  / Unclassified / 90 / 109
50.  / Verrucomicrobiae / 13, + / 16

A total of 1346 OTUs (only 57 OTUs in clone library) distributed in 46 classes (only 12 classes in clone library) were found at the Edgemont site by PhyloChip. A total of 1715 OTUs (only 85 in clone library) were assigned to 50 classes (only 14 classes in clone library) at the North Cave Hills site by PhyloChip.

-Not detected on PhyloChip

†Value indicates number of OTUs in a class obtained by PhyloChip analysis

+Class that have been retrieved both in clone library and on corresponding PhyloChip