ACRF Biomolecular Resource Facility (The BRF)

ACRF Biomolecular Resource Facility (the BRF)

John Curtin School of Medical Research

BRF Phone:+61 2 6125 4326GDU Phone:+61 2 6125 9289Sample Drop-off Address:Please print this form and complete all

BRF Email:U Email:2, Building 131, Garran Roadinformation requested. Contact us if

BRF Fax:+61 2 6125 9533Australian National Universityunsure. This form MUST be signed by

Web: GPO Box 334PI / Lab Head. Bring it to BRF with your

Canberra ACT 2601samples.

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HiSeq 2000 OrderForm

Sequencing with Customer-made Library

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Part 1: Contact Information

Date:
Customer name:
Customer address:
Phone (lab):
Phone (mobile):
Email:
PI (or Lab Head) name:
PI (or Lab Head) email:
PI (or Lab Head) signature:
By signing, you acknowledge and accept BRF charges, and terms and conditions.

Part 2: Billing Information

ANU Customers:
Please provide ANU account code.
Non ANU Customers:
A tax invoice will be emailed to the PI (or Lab Head) unless alternative information is provided here.

Part 3: Output

Please choose a data option
 / Analysed by GDU
(Please contact or 6125 9289 for a discussion of method, and for pricing).
 / Purchase 2TB hard drive from BRF ($170)
 / Supply your own hard drive

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Part 4: Libraries and sequence(Customer-made libraries)

Please provide 25 L of 2nM DNA for each lane.

The recommended method to quantify libraries is Bioanalyzer. Bring your Bioanalyzer results with your samples.

A. / Experimental design
B. / Library construction method. Please complete either (a) or (b)
(a) Illumina Method / Library Kit Name / Part Number / Lot Number
(b) Non Illumina Method / Please attach the protocol you used to this form. Customer designed adapter and primer sequence need to be included.
 Protocol attached.
C. / Sample Information. Please change this table to suit your experimental design.
Sample Submission Date:
Lane / Sample Name / Index* / Sample Origin (species/tissue) / Sequence Length
Example / 1. / BRF 1 / ATCACG / Mouse liver genomic DNA / 100 bp PE
2. / BRF 2 / PCR primer index 1 / Mouse blood DNA
3. / BRF 3 / GGCTAC / Mouse genomic DNA exon capture
4. / BRF 4 / Truseq index 11 / Mouse muscle genomic DNA
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*Index can be the index sequence or the index name if you use the standard Illumina kit.

For your reference, sequence length can be:

Single Read / Paired-end Read
50 base single read (50 bp SR) / 50 base paired-end read (75 bp PE)
100 base single read (100 bp SR) / 100 base paired-end read (100 bp PE)

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