OIE RL for «Newcastle Disease» – «Prof Ian Brown» – «United Kingdom»
OIE Reference Laboratory Reports
Activities in 2012
Address of laboratory / Avian Virology, Animal Health and Veterinary Laboratories Agency – Weybridge, Addlestone, Surrey KT15 3NB, United Kingdom
Tel.: / +44 (0)1932 357 339
Fax: / +44 (0)1932 357 239
e-mail address: /
website: / http://www.defra.gov.uk/corporate/vla/science/science-viral-ai-reflab.htm
Name (including Title) of Head of Laboratory (Responsible Official): / Christopher Hadkiss, Chief Executive
Name (including Title and Position) of OIE Reference Expert: / Professor Ian Brown
Date of submission to the OIE / 28/2/13
Instructions
This form should be used by an OIE Reference Laboratory to report activities that took place from January through December of the past year (2012), unless otherwise stated, and must be submitted by the end of January every year.
Only those activities that concern the disease (or topic) for which the laboratory is recognised by the OIE should be mentioned. The questionnaire structure follows the Terms of Reference (ToRs) for OIE Reference Laboratories, available at:
http://www.oie.int/en/our-scientific-expertise/reference-laboratories/introduction/
Each ToR (blue italicised text) has been placed as a heading covering the group of questions related to it.
Please note the red italicised text is given as guidance and should be deleted from your report and substitute with your data. Examples are based on past Annual Reports or have been invented.
The questionnaire represents a means of gathering information on activities carried out by OIE Reference Laboratories and making it available to OIE Member Countries and to the OIE Reference Laboratory network.
This annual report will remain available for consultation on the OIE web site:
(http://www.oie.int/en/our-scientific-expertise/reference-laboratories/annual-reports/):
ToR: To use, promote and disseminate diagnostic methods validated according to OIE Standards
Test recommended by the OIE / Total number of test performed last yearIndirect diagnostic tests / Nationally / Internationally
HI / 2508 / 30
Direct diagnostic tests / Nationally / Internationally
Real-time RT-PCR gene L
/ 72 / 113NDV genetic analysis
/ 10 / 37ICPI
/ 0 / 0Egg inoculation/HA
/ 1525 / 121ToR: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards.
To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease.
2. Did your laboratory produce or store imported standard reference reagents officially recognised by the OIE or other international bodies?
Yes No
3. Did your laboratory supply standard reference reagents to OIE Member Countries?
Yes No
Type of reagent available / Related diagnostic test / Produced/stored / Amount supplied nationally
(ml, mg) / Amount supplied internationally
(ml, mg) / Name of recipient OIE Member Countries and of institutions /
Control positive sera (OIE-approved international sera) / HI serological test / Produced / 10ml / UK AHVLA
PMV type antigens / HI serological test / Produced / 22ml / UK AHVLA
PMV type antigens / HI serological test / Produced / 10ml / Sweden NVI
Control positive sera (OIE-approved international sera) / HI serological test / Produced / 7ml / Sweden NVI
PMV type antigens / HI serological test / Produced / 11ml / Czech Rep SVI
Control positive sera (OIE-approved international sera) / HI serological test / Produced / 2ml / Czech Rep SVI
Control positive sera (OIE-approved international sera) / HI serological test / Produced / 1ml / Norway NVI
PMV type antigens / HI serological test / Produced / 8ml / Botswana NVL
Control positive sera (OIE-approved international sera) / HI serological test / Produced / 5ml / Botswana NVI
Control positive sera (OIE-approved international sera) / HI serological test / Produced / 3ml / Ukraine Science and Tech Centre
Control positive sera (OIE-approved international sera) / HI serological test / Produced / 5ml / Macedonia Faculty of Vet Med
4. Did your laboratory produce diagnostic reagents other than the OIE-approved standard reference reagents?
Yes No
5. Did your laboratory produce vaccines?
Yes No
6. Did your laboratory supply vaccines to OIE Member Countries?
Yes No
ToR: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases
7. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease?
Yes No
8. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease?
Yes No
Name of the new test or diagnostic method or vaccine developed / Description and References (Publication, website, etc.)An assessment of the currently-used molecular diagnostic tools was carried out in relation to the current circulating strains. / The work was presented at the Joint Eighteenth Annual Meetings of the National Laboratories for Avian Influenza and Newcastle Disease of European Union Member States 2012 (http://ec.europa.eu/food/animal/diseases/controlmeasures/avian/crls_proceedings_en.htm).
ToR: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries
9. Did your laboratory carry out diagnostic testing for other OIE Member Countries?
Yes No
Name of OIE Member Country seeking assistance / Date(dd/mm) / No. samples received for provision of diagnostic support (i.e. from surveillance campaign) / No. samples received for provision of confirmatory diagnoses
The Netherlands / 17/01 / 5
Nepal / 25/01 / 4
Nepal / 30/01 / 5
Nepal / 01/02 / 2
Sri Lanka / 03/02 / 6
Nepal / 16/02 / 4
Saudi Arabia / 05/03 / 16
Nepal / 23/03 / 5
Egypt / 26/03 / 6
Israel / 28/03 / 3
South Africa / 28/03 / 2
Saudi Arabia / 12/04 / 12
Republic of Ireland / 18/04 / 2
Cyprus / 20/04 / 1
Switzerland / 27/04 / 9
Italy / 16/05 / 6
Kuwait / 31/05 / 40
Norway / 07/06 / 1
Turkey / 14/06 / 19
Malta / 16/08 / 5
Croatia / 14/09 / 1
The Netherlands / 26/09 / 2
Nepal / 23/10 / 12
Romania / 24/10 / 1
Pakistan / 06/12 / 3
France / 13/12 / 2
Estonia / 13/12 / 3
Nepal / 27/12 / 2
10. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country?
Yes No
Name of the OIE Member Country receiving a technical consultancy / Purpose / How the advice was providedARC-Onderstepoort Veterinary Institute (ARC-OVI) / OIE-funded twinning project with AHVLA-Weybridge / Remote assistance
Botswana National Veterinary Laboratory (BNVL) / OIE-funded twinning project with AHVLA-Weybridge / Remote assistance
France (Anses) and UK (AFBI Stormont) / Ad-hoc technical support on molecular ND diagnosis and surveillance / E-mail
Philippines / To evaluate animal health conditions for importing captive live bred birds into the European Union / International mission
ToR: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations
11. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own?
Yes No
Title of the study / Duration / Purpose of the study / Partners (Institutions) / OIE Member Countries involved other than your countryEPIZONE / 6 years / Prevention and control of animal diseases to limit the risks and damage caused by those diseases in the EU and beyond. / Various EU member countries
ToR: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases
12. Did your Laboratory collect epizootiological data relevant to international disease control?
Yes No
13. Did your laboratory disseminate epizootiological data that had been processed and analysed?
Yes No
14. What method of dissemination of information is most often used by your laboratory?
(Indicate in the appropriate box the number by category)
1. Articles published in peer-reviewed journals: 3
2. International conferences: 3
3. National conferences: 0
4. Internet/website: 1Non-peer review publication (magazine article): 1
ToR: To provide scientific and technical training for personnel from OIE Member Countries
To recommend the prescribed and alternative tests or vaccines as OIE Standards
15. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries?
Yes No
a) Technical visits:
b) Seminars:
c) Hands-on training courses: 3
d) Internships (>1 month):
Type of technical training provided (a, b, c or d) / Country of origin of the expert(s) provided with training / No. participants from the corresponding countryc / The Netherlands / 1
c / Finland / 1
c / Singapore / 1
ToR: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned
16. Does your laboratory have a Quality Management System certified according to an International Standard?
Yes No
Quality management system adoptedISO 17025
17. Is your laboratory accredited by an international accreditation body?
Yes No
Test for which your laboratory is accredited / Accreditation bodyHaemagglutination inhibition test / UKAS
L-gene real-time PCR / UKAS
Newcastle disease virus – statutory sequencing / UKAS
ICPI / UKAS
Virus isolation in tissue culture for PMV / UKAS
Virus isolation in SPF eggs (via allantoic cavity) / UKAS
18. Does your laboratory maintain a “biorisk management system” for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter 1.1.3 or Manual of Diagnostic Tests for Aquatic Animals 2012, Chapter 1.1.1)
Yes No
ToR: To organise and participate in scientific meetings on behalf of the OIE
19. Did your laboratory organise scientific meetings on behalf of the OIE?
Yes x No
20. Did your laboratory participate in scientific meetings on behalf of the OIE?
Yes No
ToR: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results
21. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease?
Yes No
22. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests?
Yes No
Purpose of the proficiency tests: (validation of a diagnostic protocol: specify the test; quality control of vaccines: specify the vaccine type, etc.) / Role of your Reference Laboratory (organiser/ participant) / No. participants / Participating OIE Ref. Labs/ organising OIE Ref. Lab.Conventional and molecular panels for EU NRLs / Organiser / 53 / Belgium, Hungary, Finland, Slovakia, Latvia, Lithuania, Slovenia, Republic of Ireland, Estonia, Germany, Romania, Portugal, Czech Republic, Austria, Denmark, Sweden, Italy, Poland, Cyprus, Luxembourg, Norway, Serbia, Macedonia, Singapore, Montenegro, Egypt, Croatia and Russia (1 lab from each); UK, Bulgaria, Greece, The Netherlands, Spain, Israel, France, Ukraine and Switzerland (2 labs from each); Turkey (3 labs) and South Africa (4 labs)
23. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest?
Yes No
ToR: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results.
24. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease?
Yes No
ToR: To place expert consultants at the disposal of the OIE
25. Did your laboratory place expert consultants at the disposal of the OIE?
Yes No
Kind of consultancy / Location / Subject (facultative )Provision of comments on OIE Standards / Comments to Chapter 1.1.1 OIE Terrestrial Manual
AHVLA - Publication and dissemination of information relevant to the work of OIE (including list of scientific publications, internet publishing activities, presentations at international conferences)
¡ Scientific publications in peer-reviewed journals
1. Dundon, W.G., Heidari, A., Fusaro, A., Monne, I., Beato, M.S., Cattoli, G., Koch, G.Starick, E., Brown, I.H., Aldous, E.W., Briand, F.X., Le Gall-Recule, G., Jestin, V., Jørgensen, P.H., Berg, M., Zohari, S., Metreveli, G., Munir, M., Stah, I.K., Albina, E., Hammoumi, S., Gil, P., de Almeida, R.S., Smietanka, K., Domanska-Blicharz, K., Minta, Z., Van Borm, S., van den Berg T, Martin, A. M, Barbieri, I., and Capua, I., (2012). Genetic data from avian influenza and avian paramyxoviruses generated by the European network of excellence (EPIZONE) between 2006 and 2011: review and recommendations for surveillance. Veterinary Microbiology 27; 154 (3-4):209-21. http://dx.doi.org/10.1016/j.vetmic.2011.08.018.
2. Aldous, E.W., Fuller, C.M., Ridgeon, J.H., Irvine, R.M., Alexander, D.J., and Brown, I.H., (2012): The evolution of pigeon paramyxovirus type 1 (PPMV-1) in Great Britain: a molecular epidemiological study. Transbound Emerg Dis. 2012 Sep 11. doi: 10.1111/tbed.12006.
3. Alexander, D.J., Aldous, E.W., and Fuller, C.M., (2012): The long view: a selective review of 40 years of Newcastle disease research. Avian Pathology. 41 (4) 329 – 335. http://www.tandfonline.com/doi/abs/10.1080/03079457.2012.697991
¡ Conference Abstracts
1. Vanessa Ceeraz, William J. Cox, Scott M. Reid, Ruth Manvell, Steve C. Essen, Helen Errington, Richard M. Irvine, Ian H. Brown. Scanning surveillance and investigative diagnosis of a mixed APMV-1 and influenza A virus infection in GB poultry. 8th International Symposium on Avian Influenza, 1-4 April 2012, Royal Holloway, University of London, AHVLA, pp. 76-77.
2. Irvine, R.M., Reid, S.M., Cox, W.J., Ceeraz, V., Manvell, R., Essen, S.C., Errington, H. & Brown, I.H. (2012). Scanning surveillance and investigative diagnosis of a mixed APMV-1 and low pathogenicity avian influenza virus infection in a poultry flock in Great Britain. Proceedings of Influenza 2012: One Influenza, One World, St. Hilda’s College, Oxford, 11-13 September 2012, pg 62-63. http://lpmhealthcare.com/Influenza2012/Posters.htm
¡ Conference Proceedings
1. Brown, I.H., The Future of Livestock in Africa. Proceedings of the 5th Pan Commonwealth Veterinary Conferebnce 21 – 25 March 2011, Accra, Ghana. Journal of Commonwealth Veterinary Association, Special Issue July, 2011, Vol 27 No.2.