Comparison of Expression Vectors

Supplementary Material

Expression Platforms for Producing Eukaryotic Proteins: A Comparison of E. coliCell-Based and Wheat Germ Cell-Free Synthesis, Affinity and Solubility Tags, and Cloning Strategies

David J. Aceti, Craig A. Bingman, Russell L. Wrobel, Ronnie O. Frederick, Shin-ichi Makino, Karl W. Nichols, Sarata C. Sahu, Lai F. Bergeman, Paul G. Blommel, Claudia C. Cornilescu, Katarzyna A. Gromek, Kory D. Seder, Soyoon Hwang, John G. Primm, Grzegorz Sabat, Frank C. Vojtik, Brian F. Volkman, Zsolt Zolnai, George N. Phillips, Jr., John L. Markley, and Brian G. Fox.

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D. J. Aceti • C. A. Bingman • R. L. Wrobel • R. O. Frederick • S. Makino • K. W. Nichols • S. C. Sahu • L. F. Bergeman • P. G. Blommel • C. C. Cornilescu • K. A. Gromek • K. D. Seder • S. Hwang • J. G. Primm • G. Sabat • F. C. Vojtik • Z. Zolnai • G. N. Phillips, Jr. • J. L. Markley • B. G. Fox

The Center for Eukaryotic Structural Genomics, Nuclear Magnetic Resonance Facility at Madison, Department of Biochemistry, University ofWisconsin at Madison, 433 Babcock Dr., Madison, Wisconsin 53706

B. F. Volkman

The Center for Eukaryotic Structural Genomics, Department of Biochemistry, Medical College of Wisconsin, 8701 W. Watertown Plank Rd., Milwaukee, WI 53226

S. C. Sahu •C. C. Cornilescu •J. L. Markley

Nuclear Magnetic Resonance Facility at Madison, Department of Biochemistry, University ofWisconsin at Madison, 433 Babcock Dr., Madison, Wisconsin 53706

C. A. Bingman • G. N. Phillips, Jr.

Natural Product Biosynthesis,BioSciences at Rice and Department of Chemistry, Rice University, Houston, TX 77251

D. J. Aceti • C. A. Bingman • R. L. Wrobel • R. O. Frederick • S. Makino •K. A. Gromek •J. G. Primm • J. L. Markley

Mitochondrial Protein Partnership, Department of Biochemistry, University of Wisconsin at Madison, 433 Babcock Dr., Madison, Wisconsin 53706

D. J. Aceti • C. A. Bingman • R. L. Wrobel • R. O. Frederick • S. Makino • K. A. Gromek • J. G. Primm • B. G. Fox.

Transmembrane Protein Center, Department of Biochemistry, University of Wisconsin at Madison, 433 Babcock Dr., Madison, Wisconsin 53706

G. N. Phillips, Jr.

Current affiliation: BioSciences at Rice and Department of Chemistry, Rice University, Houston, TX 77251

B. G. Fox

Corresponding author: Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Dr., Madison, Wisconsin 53706, Tel:(608) 262-9708, E-mail: , FAX: 608-262-4693

Online Resource 2 contains the components and concentrations of the UW192 protein crystallization screen.

1

Online Resource 2 The UW192 protein crystallization screen.

Well Label / pH Observed / Total Vol. (ul) / H2O Vol. (ul) / Precipitant / Conc. / Unit / Vol. (ul) / SSID: Precipitant / Conc. / Unit / Vol. (ul) / Salt / Conc. / Unit / Vol. (ul) / SSID: Salt / Conc. / Unit / Vol. (ul) / Buffer / Conc. / Unit / Vol. (ul)
A1 / 4.2 / 1000 / 410 / 40.0 % MEPEG 5K / 17.6 / % / 440 / EMPTY / 0 / 0 / 4.0 M NaCl / 200 / mM / 50 / EMPTY / 0 / 0 / 1.0 M Succinate pH 4.0 / 100 / mM / 100
A2 / 4.5 / 1000 / 600 / 40.0 % MEPEG 5K / 10.4 / % / 260 / EMPTY / 0 / 0 / 2.8 M MgSO4 / 112 / mM / 40 / EMPTY / 0 / 0 / 1.0 M Na Acetate pH 4.5 / 100 / mM / 100
A3 / 5 / 1000 / 510 / 40.0 % MEPEG 5K / 17.6 / % / 440 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 1.0 M NaAcetate pH 5.0 / 50 / mM / 50
A4 / 5.4 / 1000 / 582.9 / 40.0 % MEPEG 5K / 10.4 / % / 260 / EMPTY / 0 / 0 / 3.5 M (NH4)2SO4 / 200 / mM / 57.14 / EMPTY / 0 / 0 / 1.0 M MES/Acetate pH 5.5 / 100 / mM / 100
A5 / 5.6 / 1000 / 340 / 40.0 % MEPEG 5K / 18.4 / % / 460 / EMPTY / 0 / 0 / 2.0 M KBr / 200 / mM / 100 / EMPTY / 0 / 0 / 1.0 M MES/Acetate pH 5.5 / 100 / mM / 100
A6 / 5.8 / 1000 / 540 / 40.0 % MEPEG 5K / 11.2 / % / 280 / EMPTY / 0 / 0 / 4.0 M Na Formate / 320 / mM / 80 / EMPTY / 0 / 0 / 1.0 M MES pH 6.0 / 100 / mM / 100
A7 / 5.7 / 1000 / 220 / 40.0 % MEPEG 5K / 24 / % / 600 / EMPTY / 0 / 0 / 2.0 M CaCl2 / 160 / mM / 80 / EMPTY / 0 / 0 / 1.0 M MES pH 6.0 / 100 / mM / 100
A8 / 6.5 / 1000 / 540 / 40.0 % MEPEG 5K / 10.4 / % / 260 / EMPTY / 0 / 0 / 3.0 M TMACl / 300 / mM / 100 / EMPTY / 0 / 0 / 1.0 M BisTris pH 6.5 / 100 / mM / 100
A9 / 5.7 / 1000 / 85 / 40.0 % MEPEG 5K / 29.6 / % / 740 / EMPTY / 0 / 0 / 1.6 M Na3 Citrate / 120 / mM / 75 / EMPTY / 0 / 0 / 1.0 M MES pH 6.0 / 100 / mM / 100
A10 / 6.7 / 1000 / 620 / 40.0 % MEPEG 5K / 10.4 / % / 260 / EMPTY / 0 / 0 / 4.0 M NaCl / 80 / mM / 20 / EMPTY / 0 / 0 / 1.0 M MOPS pH 7.0 / 100 / mM / 100
A11 / 6.8 / 1000 / 400 / 40.0 % MEPEG 5K / 16 / % / 400 / EMPTY / 0 / 0 / 2.0 M Glycine / 200 / mM / 100 / EMPTY / 0 / 0 / 1.0 M BTP pH 7.0 / 100 / mM / 100
A12 / 6.8 / 1000 / 380 / 40.0 % MEPEG 5K / 17.6 / % / 440 / EMPTY / 0 / 0 / 2.0 M K Glutamate / 160 / mM / 80 / EMPTY / 0 / 0 / 1.0 M MOPS pH 7.0 / 100 / mM / 100
B1 / 5 / 1000 / 133.3 / 60.0 % DMPEG 500 / 40 / % / 666.67 / EMPTY / 0 / 0 / 2.0 M AmSCN / 200 / mM / 100 / EMPTY / 0 / 0 / 1.0 M Na Acetate pH 4.5 / 100 / mM / 100
B2 / 6 / 1000 / 140 / 60.0 % DMPEG 500 / 36 / % / 600 / EMPTY / 0 / 0 / 3.0 M TMACl / 480 / mM / 160 / EMPTY / 0 / 0 / 1.0 M PIPES pH 6.5 / 100 / mM / 100
B3 / 6.5 / 1000 / 126.7 / 60.0 % DMPEG 500 / 44 / % / 733.33 / EMPTY / 0 / 0 / 4.0 M NaCl / 160 / mM / 40 / EMPTY / 0 / 0 / 1.0 M BisTris pH 6.5 / 100 / mM / 100
B4 / 7 / 1000 / 473.3 / 60.0 % DMPEG 500 / 24 / % / 400 / EMPTY / 0 / 0 / 3.0 M NaH2PO4 / 34 / mM / 11.33 / 712: 3.0 M K2HPO4 / 46 / mM / 15.3 / 1.0 M MOPS pH 7.0 / 100 / mM / 100
B5 / 7.5 / 1000 / 193.3 / 60.0 % DMPEG 500 / 40 / % / 666.67 / EMPTY / 0 / 0 / 3.4 M Na Malonate / 136 / mM / 40 / EMPTY / 0 / 0 / 1.0 M HEPES pH 7.5 / 100 / mM / 100
B6 / 8.5 / 1000 / 290 / 60.0 % DMPEG 500 / 36 / % / 600 / EMPTY / 0 / 0 / 2.0 M MgCl2 / 20 / mM / 10 / EMPTY / 0 / 0 / 1.0 M HEPPS pH 8.5 / 100 / mM / 100
B7 / 6 / 1000 / 305 / 40.0 % MEPEG 5K / 11 / % / 275 / EMPTY / 0 / 0 / 3.0 M TMACl / 960 / mM / 320 / EMPTY / 0 / 0 / 1.0 M PIPES pH 6.5 / 100 / mM / 100
B8 / 7 / 1000 / 410 / 40.0 % MEPEG 5K / 10 / % / 250 / EMPTY / 0 / 0 / 4.0 M NaCl / 960 / mM / 240 / EMPTY / 0 / 0 / 1.0 M MOPS pH 7.0 / 100 / mM / 100
B9 / 8 / 1000 / 195 / 40.0 % MEPEG 5K / 9 / % / 225 / EMPTY / 0 / 0 / 3.0 M TMACl / ### / mM / 480 / EMPTY / 0 / 0 / 1.0 M Triethanolamine pH 8.0 / 100 / mM / 100
B10 / 4.5 / 1000 / 346.7 / 75.0 % MPD / 40 / % / 533.33 / EMPTY / 0 / 0 / 4.0 M NaCl / 80 / mM / 20 / EMPTY / 0 / 0 / 1.0 M Na Acetate pH 4.5 / 100 / mM / 100
B11 / 5 / 1000 / 16.67 / 75.0 % MPD / 70 / % / 933.33 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 1.0 M NaAcetate pH 5.0 / 50 / mM / 50
B12 / 5.5 / 1000 / 440 / 75.0 % MPD / 30 / % / 400 / EMPTY / 0 / 0 / 2.0 M MgCl2 / 120 / mM / 60 / EMPTY / 0 / 0 / 1.0 M MES/Acetate pH 5.5 / 100 / mM / 100
C1 / 7.8 / 1000 / 400 / 40.0 % MEPEG 5K / 17.6 / % / 440 / EMPTY / 0 / 0 / 2.0 M AmSCN / 120 / mM / 60 / EMPTY / 0 / 0 / 1.0 M Triethanolamine pH 7.5 / 100 / mM / 100
C2 / 7.3 / 1000 / 200 / 40.0 % MEPEG 5K / 24 / % / 600 / EMPTY / 0 / 0 / 2.0 M KNO3 / 200 / mM / 100 / EMPTY / 0 / 0 / 1.0 M HEPES pH 7.5 / 100 / mM / 100
C3 / 7.4 / 1000 / 230 / 40.0 % MEPEG 5K / 28.8 / % / 720 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 1.0 M HEPES pH 7.5 / 50 / mM / 50
C4 / 8.2 / 1000 / 579.2 / 40.0 % MEPEG 5K / 9.6 / % / 240 / EMPTY / 0 / 0 / 2.5 M (NH4)H2PO4 / 12 / mM / 4.8 / 989: 3.0 M (NH4)2HPO4 / 228 / mM / 76 / 1.0 M Triethanolamine pH 8.0 / 100 / mM / 100
C5 / 8.3 / 1000 / 260 / 40.0 % MEPEG 5K / 24 / % / 600 / EMPTY / 0 / 0 / 4.0 M NaCl / 160 / mM / 40 / EMPTY / 0 / 0 / 1.0 M Triethanolamine pH 8.0 / 100 / mM / 100
C6 / 7.8 / 1000 / 690 / 40.0 % MEPEG 5K / 10.4 / % / 260 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 1.0 M HEPPS pH 8.5 / 50 / mM / 50
C7 / 8.8 / 1000 / 282.1 / 40.0 % MEPEG 5K / 24 / % / 600 / EMPTY / 0 / 0 / 2.8 M MgSO4 / 50 / mM / 17.86 / EMPTY / 0 / 0 / 1.0 M CHES pH 9.0 / 100 / mM / 100
C8 / 9 / 1000 / 600 / 40.0 % MEPEG 5K / 9.6 / % / 240 / EMPTY / 0 / 0 / 2.0 M MgCl2 / 120 / mM / 60 / EMPTY / 0 / 0 / 1.0 M BTP pH 9.0 / 100 / mM / 100
C9 / 8.9 / 1000 / 420 / 40.0 % MEPEG 5K / 16 / % / 400 / EMPTY / 0 / 0 / 2.0 M KCl / 160 / mM / 80 / EMPTY / 0 / 0 / 1.0 M CHES pH 9.0 / 100 / mM / 100
C10 / 4.2 / 1000 / 710 / 40.0 % PEG 4K / 9.6 / % / 240 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 1.0 M Succinate pH 4.0 / 50 / mM / 50
C11 / 5.1 / 1000 / 200 / 40.0 % PEG 4K / 24 / % / 600 / EMPTY / 0 / 0 / 4.0 M NaCl / 400 / mM / 100 / EMPTY / 0 / 0 / 1.0 M NaAcetate pH 5.0 / 100 / mM / 100
C12 / 5.5 / 1000 / 600 / 40.0 % PEG 4K / 10.4 / % / 260 / EMPTY / 0 / 0 / 2.0 M Glycine / 80 / mM / 40 / EMPTY / 0 / 0 / 1.0 M MES/Acetate pH 5.5 / 100 / mM / 100
D1 / 6 / 1000 / 160 / 75.0 % MPD / 60 / % / 800 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 1.0 M MES pH 6.0 / 40 / mM / 40
D2 / 6.5 / 1000 / 173.3 / 75.0 % MPD / 50 / % / 666.67 / EMPTY / 0 / 0 / 4.0 M NaCl / 240 / mM / 60 / EMPTY / 0 / 0 / 1.0 M BisTris pH 6.5 / 100 / mM / 100
D3 / 7 / 1000 / 360 / 75.0 % MPD / 36 / % / 480 / EMPTY / 0 / 0 / 2.0 M CaCl2 / 120 / mM / 60 / EMPTY / 0 / 0 / 1.0 M BisTris pH 6.5 / 100 / mM / 100
D4 / 7.5 / 1000 / 362.9 / 75.0 % MPD / 36 / % / 480 / EMPTY / 0 / 0 / 3.5 M (NH4)2SO4 / 200 / mM / 57.14 / EMPTY / 0 / 0 / 1.0 M HEPES pH 7.5 / 100 / mM / 100
D5 / 8.5 / 1000 / 173.3 / 75.0 % MPD / 50 / % / 666.67 / EMPTY / 0 / 0 / 2.0 M Li2SO4 / 120 / mM / 60 / EMPTY / 0 / 0 / 1.0 M CHES pH 9.0 / 100 / mM / 100
D6 / 5 / 1000 / 380 / 50.0 % PEG 400 / 24 / % / 480 / EMPTY / 0 / 0 / 2.0 M MgCl2 / 80 / mM / 40 / EMPTY / 0 / 0 / 1.0 M NaAcetate pH 5.0 / 100 / mM / 100
D7 / 5.5 / 1000 / 100 / 50.0 % PEG 400 / 36 / % / 720 / EMPTY / 0 / 0 / 4.0 M NaCl / 320 / mM / 80 / EMPTY / 0 / 0 / 1.0 M MES/Acetate pH 5.5 / 100 / mM / 100
D8 / 6.5 / 1000 / 320 / 50.0 % PEG 400 / 24 / % / 480 / EMPTY / 0 / 0 / 2.0 M MgCl2 / 200 / mM / 100 / EMPTY / 0 / 0 / 1.0 M MOPS pH 7.0 / 100 / mM / 100
D9 / 7 / 1000 / 105.7 / 50.0 % PEG 400 / 38 / % / 760 / EMPTY / 0 / 0 / 3.5 M (NH4)2SO4 / 120 / mM / 34.29 / EMPTY / 0 / 0 / 1.0 M MOPS pH 7.0 / 100 / mM / 100
D10 / 7.5 / 1000 / 0 / 50.0 % PEG 400 / 40 / % / 800 / EMPTY / 0 / 0 / 2.0 M KNO3 / 200 / mM / 100 / EMPTY / 0 / 0 / 1.0 M HEPES pH 7.5 / 100 / mM / 100
D11 / 8 / 1000 / 80 / 50.0 % PEG 400 / 36 / % / 720 / EMPTY / 0 / 0 / 2.0 M Li2SO4 / 200 / mM / 100 / EMPTY / 0 / 0 / 1.0 M Triethanolamine pH 8.0 / 100 / mM / 100
D12 / 9 / 1000 / 0 / 50.0 % PEG 400 / 44 / % / 880 / EMPTY / 0 / 0 / 4.0 M NaCl / 80 / mM / 20 / EMPTY / 0 / 0 / 1.0 M BTP pH 9.0 / 100 / mM / 100
E1 / 5.7 / 1000 / 350 / 40.0 % PEG 4K / 24 / % / 600 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 1.0 M MES/Acetate pH 5.5 / 50 / mM / 50
E2 / 5.7 / 1000 / 420 / 40.0 % PEG 4K / 17.6 / % / 440 / EMPTY / 0 / 0 / 4.0 M NaCl / 160 / mM / 40 / EMPTY / 0 / 0 / 1.0 M MES pH 6.0 / 100 / mM / 100
E3 / 6.3 / 1000 / 690 / 40.0 % PEG 4K / 10.4 / % / 260 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 1.0 M BisTris pH 6.5 / 50 / mM / 50
E4 / 6.4 / 1000 / 360 / 40.0 % PEG 4K / 18.4 / % / 460 / EMPTY / 0 / 0 / 2.0 M KBr / 160 / mM / 80 / EMPTY / 0 / 0 / 1.0 M BisTris pH 6.5 / 100 / mM / 100
E5 / 6.7 / 1000 / 490 / 40.0 % PEG 4K / 18.4 / % / 460 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 1.0 M BTP pH 7.0 / 50 / mM / 50
E6 / 6.9 / 1000 / 120 / 40.0 % PEG 4K / 29.6 / % / 740 / EMPTY / 0 / 0 / 3.4 M Na Malonate / 136 / mM / 40 / EMPTY / 0 / 0 / 1.0 M BTP pH 7.0 / 100 / mM / 100
E7 / 7.3 / 1000 / 540 / 40.0 % PEG 4K / 10.4 / % / 260 / EMPTY / 0 / 0 / 4.0 M NaCl / 400 / mM / 100 / EMPTY / 0 / 0 / 1.0 M HEPES pH 7.5 / 100 / mM / 100
E8 / 7.3 / 1000 / 290 / 40.0 % PEG 4K / 24 / % / 600 / EMPTY / 0 / 0 / 2.0 M MgCl2 / 20 / mM / 10 / EMPTY / 0 / 0 / 1.0 M HEPES pH 7.5 / 100 / mM / 100
E9 / 8.2 / 1000 / 470 / 40.0 % PEG 4K / 19.2 / % / 480 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 1.0 M Triethanolamine pH 7.5 / 50 / mM / 50
E10 / 8.2 / 1000 / 360 / 40.0 % PEG 4K / 20 / % / 500 / EMPTY / 0 / 0 / 2.0 M CaCl2 / 80 / mM / 40 / EMPTY / 0 / 0 / 1.0 M HEPPS pH 8.5 / 100 / mM / 100
E11 / 8.6 / 1000 / 557.1 / 40.0 % PEG 4K / 12.8 / % / 320 / EMPTY / 0 / 0 / 3.5 M (NH4)2SO4 / 80 / mM / 22.86 / EMPTY / 0 / 0 / 1.0 M BTP pH 9.0 / 100 / mM / 100
E12 / 4.3 / 1000 / 200 / 40.0 % PEG 8K / 24.8 / % / 620 / EMPTY / 0 / 0 / 2.0 M KCl / 160 / mM / 80 / EMPTY / 0 / 0 / 1.0 M Na Acetate pH 4.5 / 100 / mM / 100
F1 / 3.9 / 1000 / 300 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 3.5 M (NH4)2SO4 / ### / mM / 600 / EMPTY / 0 / 0 / 1.0 M Succinate pH 4.0 / 100 / mM / 100
F2 / 4.5 / 1000 / 333.3 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 3.0 M NaH2PO4 / ### / mM / 666.7 / EMPTY / 0 / 0 / EMPTY / 0 / 0
F3 / 4.4 / 1000 / 520 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 3.5 M (NH4)2SO4 / ### / mM / 380 / EMPTY / 0 / 0 / 1.0 M NaAcetate pH 5.0 / 100 / mM / 100
F4 / 4.7 / 1000 / 650 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 4.0 M NaCl / ### / mM / 250 / EMPTY / 0 / 0 / 1.0 M NaAcetate pH 5.0 / 100 / mM / 100
F5 / 5.1 / 1000 / 360.7 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 2.8 M MgSO4 / ### / mM / 539.3 / EMPTY / 0 / 0 / 1.0 M MES/Acetate pH 5.5 / 100 / mM / 100
F6 / 5.7 / 1000 / 180 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 3.5 M (NH4)2SO4 / ### / mM / 720 / EMPTY / 0 / 0 / 1.0 M MES/Acetate pH 5.5 / 100 / mM / 100
F7 / 6.1 / 1000 / 216 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 2.5 M (NH4)H2PO4 / ### / mM / 704 / 989: 3.0 M (NH4)2HPO4 / 240 / mM / 80 / EMPTY / 0 / 0
F8 / 6 / 1000 / 600 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 3.5 M (NH4)2SO4 / ### / mM / 300 / EMPTY / 0 / 0 / 1.0 M MES pH 6.0 / 100 / mM / 100
F9 / 7 / 1000 / 185.7 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 3.5 M (NH4)2SO4 / ### / mM / 714.3 / EMPTY / 0 / 0 / 1.0 M BisTris pH 6.5 / 100 / mM / 100
F10 / 6.5 / 1000 / 100 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 3.4 M Na Malonate / ### / mM / 800 / EMPTY / 0 / 0 / 1.0 M PIPES pH 6.5 / 100 / mM / 100
F11 / 5.8 / 1000 / 600 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 3.4 M Na Malonate / ### / mM / 300 / EMPTY / 0 / 0 / 1.0 M PIPES pH 6.5 / 100 / mM / 100
F12 / 7.5 / 1000 / 14.29 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 3.5 M (NH4)2SO4 / ### / mM / 885.7 / EMPTY / 0 / 0 / 1.0 M BTP pH 7.0 / 100 / mM / 100
G1 / 4.9 / 1000 / 620 / 40.0 % PEG 8K / 9.6 / % / 240 / EMPTY / 0 / 0 / 2.0 M CaCl2 / 80 / mM / 40 / EMPTY / 0 / 0 / 1.0 M NaAcetate pH 5.0 / 100 / mM / 100
G2 / 6.2 / 1000 / 400 / 40.0 % PEG 8K / 18.4 / % / 460 / EMPTY / 0 / 0 / 3.0 M NaH2PO4 / 106 / mM / 35.33 / 712: 3.0 M K2HPO4 / 14 / mM / 4.67 / 1.0 M MES pH 6.0 / 100 / mM / 100
G3 / 6.2 / 1000 / 240 / 40.0 % PEG 8K / 24.8 / % / 620 / EMPTY / 0 / 0 / 2.0 M MgCl2 / 80 / mM / 40 / EMPTY / 0 / 0 / 1.0 M PIPES pH 6.5 / 100 / mM / 100
G4 / 6.2 / 1000 / 560 / 40.0 % PEG 8K / 11.2 / % / 280 / EMPTY / 0 / 0 / 2.0 M Li2SO4 / 120 / mM / 60 / EMPTY / 0 / 0 / 1.0 M PIPES pH 6.5 / 100 / mM / 100
G5 / 6.4 / 1000 / 490 / 40.0 % PEG 8K / 18.4 / % / 460 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 1.0 M PIPES pH 6.5 / 50 / mM / 50
G6 / 6.7 / 1000 / 710 / 40.0 % PEG 8K / 9.6 / % / 240 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 1.0 M BTP pH 7.0 / 50 / mM / 50
G7 / 6.9 / 1000 / 214.4 / 40.0 % PEG 8K / 25.6 / % / 640 / EMPTY / 0 / 0 / 2.5 M (NH4)H2PO4 / 84 / mM / 33.6 / 989: 3.0 M (NH4)2HPO4 / 36 / mM / 12 / 1.0 M PIPES pH 6.5 / 100 / mM / 100
G8 / 7.8 / 1000 / 420 / 40.0 % PEG 8K / 16 / % / 400 / EMPTY / 0 / 0 / 3.4 M Na Malonate / 272 / mM / 80 / EMPTY / 0 / 0 / 1.0 M Triethanolamine pH 7.5 / 100 / mM / 100
G9 / 8.3 / 1000 / 560 / 40.0 % PEG 8K / 10.4 / % / 260 / EMPTY / 0 / 0 / 2.8 M MgSO4 / 224 / mM / 80 / EMPTY / 0 / 0 / 1.0 M Triethanolamine pH 8.0 / 100 / mM / 100
G10 / 8.3 / 1000 / 320 / 40.0 % PEG 8K / 17.6 / % / 440 / EMPTY / 0 / 0 / 3.0 M TMACl / 420 / mM / 140 / EMPTY / 0 / 0 / 1.0 M Triethanolamine pH 8.0 / 100 / mM / 100
G11 / 8.1 / 1000 / 380 / 40.0 % PEG 8K / 14.4 / % / 360 / EMPTY / 0 / 0 / 2.0 M AmSCN / 320 / mM / 160 / EMPTY / 0 / 0 / 1.0 M HEPPS pH 8.5 / 100 / mM / 100
G12 / 9 / 1000 / 350 / 40.0 % PEG 8K / 24 / % / 600 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 1.0 M CHES pH 9.0 / 50 / mM / 50
H1 / 7.1 / 1000 / 300 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 3.4 M Na Malonate / ### / mM / 600 / EMPTY / 0 / 0 / 1.0 M MOPS pH 7.0 / 100 / mM / 100
H2 / 7 / 1000 / 631.6 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 2.5 M (NH4)H2PO4 / 431 / mM / 172.4 / 989: 3.0 M (NH4)2HPO4 / 588 / mM / 196 / EMPTY / 0 / 0
H3 / 7.1 / 1000 / 400 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 4.0 M NaCl / ### / mM / 500 / EMPTY / 0 / 0 / 1.0 M BTP pH 7.0 / 100 / mM / 100
H4 / 7.6 / 1000 / 240 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 3.5 M (NH4)2SO4 / ### / mM / 660 / EMPTY / 0 / 0 / 1.0 M HEPES pH 7.5 / 100 / mM / 100
H5 / 7.9 / 1000 / 600 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 3.4 M Na Malonate / ### / mM / 300 / EMPTY / 0 / 0 / 1.0 M Triethanolamine pH 7.5 / 100 / mM / 100
H6 / 7.5 / 1000 / 100 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 3.4 M Na Malonate / ### / mM / 800 / EMPTY / 0 / 0 / 1.0 M HEPES pH 7.5 / 100 / mM / 100
H7 / 8 / 1000 / 400 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 4.0 M Na Formate / ### / mM / 500 / EMPTY / 0 / 0 / 1.0 M Triethanolamine pH 8.0 / 100 / mM / 100
H8 / 8 / 1000 / 89.33 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 2.5 M (NH4)H2PO4 / 160 / mM / 64 / 989: 3.0 M (NH4)2HPO4 / ### / mM / 747 / 1.0 M HEPPS pH 8.5 / 100 / mM / 100
H9 / 7.9 / 1000 / 600 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 3.5 M (NH4)2SO4 / ### / mM / 300 / EMPTY / 0 / 0 / 1.0 M CHES pH 8.5 / 100 / mM / 100
H10 / 8.3 / 1000 / 220 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 3.0 M TMACl / ### / mM / 680 / EMPTY / 0 / 0 / 1.0 M HEPPS pH 8.5 / 100 / mM / 100
H11 / 8.3 / 1000 / 400 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 3.5 M (NH4)2SO4 / ### / mM / 500 / EMPTY / 0 / 0 / 1.0 M BTP pH 9.0 / 100 / mM / 100
H12 / 9 / 1000 / 539.3 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 2.8 M MgSO4 / ### / mM / 360.7 / EMPTY / 0 / 0 / 1.0 M BTP pH 9.0 / 100 / mM / 100
I1 / 4.8 / 1000 / 236.7 / 40.0 % PEG 4K / 16 / % / 400 / 698: 75.0 % MPD / 16 / % / 213 / 4.0 M NaCl / 200 / mM / 50 / EMPTY / 0 / 0 / 1.0 M Na Acetate pH 4.5 / 100 / mM / 100
I2 / 5.6 / 1000 / 333.3 / 40.0 % PEG 4K / 10 / % / 250 / 698: 75.0 % MPD / 20 / % / 267 / 2.0 M CaCl2 / 100 / mM / 50 / EMPTY / 0 / 0 / 1.0 M MES pH 6.0 / 100 / mM / 100
I3 / 6.8 / 1000 / 320.8 / 40.0 % PEG 4K / 10 / % / 250 / 698: 75.0 % MPD / 20 / % / 267 / 1.6 M Na3 Citrate / 100 / mM / 62.5 / EMPTY / 0 / 0 / 1.0 M MOPS pH 7.0 / 100 / mM / 100
I4 / 8.3 / 1000 / 266.7 / 40.0 % PEG 4K / 16 / % / 400 / 698: 75.0 % MPD / 10 / % / 133 / 2.0 M KBr / 200 / mM / 100 / EMPTY / 0 / 0 / 1.0 M Triethanolamine pH 8.0 / 100 / mM / 100
I5 / 4.9 / 1000 / 200 / 40.0 % PEG 4K / 16 / % / 400 / 700: 50.0 % PEG 400 / 10 / % / 200 / 2.0 M Glycine / 200 / mM / 100 / EMPTY / 0 / 0 / 1.0 M Na Acetate pH 4.5 / 100 / mM / 100
I6 / 6.3 / 1000 / 271.4 / 40.0 % PEG 4K / 16 / % / 400 / 700: 50.0 % PEG 400 / 10 / % / 200 / 3.5 M (NH4)2SO4 / 100 / mM / 28.57 / EMPTY / 0 / 0 / 1.0 M PIPES pH 6.5 / 100 / mM / 100
I7 / 7.3 / 1000 / 270 / 40.0 % PEG 4K / 10 / % / 250 / 700: 50.0 % PEG 400 / 16 / % / 320 / 3.4 M Na Malonate / 204 / mM / 60 / EMPTY / 0 / 0 / 1.0 M Triethanolamine pH 7.5 / 100 / mM / 100
I8 / 8.1 / 1000 / 250 / 40.0 % PEG 4K / 16 / % / 400 / 700: 50.0 % PEG 400 / 10 / % / 200 / 2.0 M Li2SO4 / 100 / mM / 50 / EMPTY / 0 / 0 / 1.0 M HEPPS pH 8.5 / 100 / mM / 100
I9 / 8.8 / 1000 / 230 / 40.0 % PEG 4K / 10 / % / 250 / 700: 50.0 % PEG 400 / 20 / % / 400 / 2.0 M MgCl2 / 40 / mM / 20 / EMPTY / 0 / 0 / 1.0 M CHES pH 9.0 / 100 / mM / 100
I10 / 4.7 / 1000 / 290 / 50.0 % PEG 1.5K / 28 / % / 560 / EMPTY / 0 / 0 / 2.0 M Li2SO4 / 100 / mM / 50 / EMPTY / 0 / 0 / 1.0 M Na Acetate pH 4.5 / 100 / mM / 100
I11 / 4.9 / 1000 / 320 / 50.0 % PEG 1.5K / 25 / % / 500 / EMPTY / 0 / 0 / 2.0 M MgCl2 / 160 / mM / 80 / EMPTY / 0 / 0 / 1.0 M NaAcetate pH 5.0 / 100 / mM / 100
I12 / 5.7 / 1000 / 440 / 50.0 % PEG 1.5K / 25 / % / 500 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 1.0 M MES/Acetate pH 5.5 / 60 / mM / 60
J1 / 7 / 1000 / 666.7 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 3.0 M NaH2PO4 / 423 / mM / 141 / 712: 3.0 M K2HPO4 / 577 / mM / 192 / EMPTY / 0 / 0
J2 / 5 / 1000 / 401 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 3.0 M NaH2PO4 / ### / mM / 591 / 712: 3.0 M K2HPO4 / 24 / mM / 8 / EMPTY / 0 / 0
J3 / 8 / 1000 / 400 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 3.0 M NaH2PO4 / 123 / mM / 41 / 712: 3.0 M K2HPO4 / ### / mM / 559 / EMPTY / 0 / 0
J4 / 7 / 1000 / 483.3 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 1.2 M Na Succinate pH 7.0 / 0.5 / M / 416.7 / EMPTY / 0 / 0 / 1.0 M BTP pH 7.0 / 100 / mM / 100
J5 / 4.5 / 1000 / 500 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 2.0 M Li2SO4 / 800 / mM / 400 / EMPTY / 0 / 0 / 1.0 M Na Acetate pH 4.5 / 100 / mM / 100
J6 / 8.5 / 1000 / 500 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 2.0 M Li2SO4 / 800 / mM / 400 / EMPTY / 0 / 0 / 1.0 M Tris pH 8.5 / 100 / mM / 100
J7 / 7 / 1000 / 150 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 2.0 M Li2SO4 / ### / mM / 750 / EMPTY / 0 / 0 / 1.0 M BTP pH 7.0 / 100 / mM / 100
J8 / 7 / 1000 / 257.1 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 2.8 M MgSO4 / ### / mM / 642.9 / EMPTY / 0 / 0 / 1.0 M BTP pH 7.0 / 100 / mM / 100
J9 / 8.5 / 1000 / 433.3 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 1.5 M (NH4)2 tartarate / 700 / mM / 466.7 / EMPTY / 0 / 0 / 1.0 M Tris pH 8.5 / 100 / mM / 100
J10 / 7 / 1000 / 33.33 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 1.5 M (NH4)2 tartarate / ### / mM / 866.7 / EMPTY / 0 / 0 / 1.0 M BTP pH 7.0 / 100 / mM / 100
J11 / 6 / 1000 / 297.1 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 3.4 M Na Malonate / ### / mM / 602.9 / EMPTY / 0 / 0 / 1.0 M MES pH 6.0 / 100 / mM / 100
J12 / 9 / 1000 / 458.8 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 3.4 M Na Malonate / ### / mM / 441.2 / EMPTY / 0 / 0 / 1.0 M BTP pH 9.0 / 100 / mM / 100
K1 / 6 / 1000 / 200 / 50.0 % PEG 1.5K / 30 / % / 600 / EMPTY / 0 / 0 / 2.0 M K Glutamate / 200 / mM / 100 / EMPTY / 0 / 0 / 1.0 M MES pH 6.0 / 100 / mM / 100
K2 / 6.3 / 1000 / 433.3 / 50.0 % PEG 1.5K / 20 / % / 400 / EMPTY / 0 / 0 / 3.0 M NaH2PO4 / 176 / mM / 58.67 / 712: 3.0 M K2HPO4 / 24 / mM / 8 / 1.0 M MES pH 6.0 / 100 / mM / 100
K3 / 6.4 / 1000 / 400 / 50.0 % PEG 1.5K / 25 / % / 500 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 1.0 M PIPES pH 6.5 / 100 / mM / 100
K4 / 6.4 / 1000 / 393.3 / 50.0 % PEG 1.5K / 20 / % / 400 / EMPTY / 0 / 0 / 3.0 M TMACl / 320 / mM / 106.7 / EMPTY / 0 / 0 / 1.0 M BisTris pH 6.5 / 100 / mM / 100
K5 / 6.8 / 1000 / 500 / 50.0 % PEG 1.5K / 18 / % / 360 / EMPTY / 0 / 0 / 3.4 M Na Malonate / 136 / mM / 40 / EMPTY / 0 / 0 / 1.0 M BTP pH 7.0 / 100 / mM / 100
K6 / 7.8 / 1000 / 160 / 50.0 % PEG 1.5K / 32 / % / 640 / EMPTY / 0 / 0 / 2.0 M KBr / 200 / mM / 100 / EMPTY / 0 / 0 / 1.0 M Triethanolamine pH 7.5 / 100 / mM / 100
K7 / 7.1 / 1000 / 431.4 / 50.0 % PEG 1.5K / 20 / % / 400 / EMPTY / 0 / 0 / 3.5 M (NH4)2SO4 / 240 / mM / 68.57 / EMPTY / 0 / 0 / 1.0 M BTP pH 7.0 / 100 / mM / 100
K8 / 7.3 / 1000 / 540 / 50.0 % PEG 1.5K / 20 / % / 400 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 1.0 M HEPES pH 7.5 / 60 / mM / 60
K9 / 7.7 / 1000 / 380 / 50.0 % PEG 1.5K / 24 / % / 480 / EMPTY / 0 / 0 / 2.0 M Li2SO4 / 80 / mM / 40 / EMPTY / 0 / 0 / 1.0 M Triethanolamine pH 7.5 / 100 / mM / 100
K10 / 8.1 / 1000 / 440 / 50.0 % PEG 1.5K / 20 / % / 400 / EMPTY / 0 / 0 / 2.0 M Glycine / 120 / mM / 60 / EMPTY / 0 / 0 / 1.0 M Triethanolamine pH 8.0 / 100 / mM / 100
K11 / 8.2 / 1000 / 80 / 50.0 % PEG 1.5K / 36 / % / 720 / EMPTY / 0 / 0 / 2.0 M K Glutamate / 200 / mM / 100 / EMPTY / 0 / 0 / 1.0 M Triethanolamine pH 8.0 / 100 / mM / 100
K12 / 8.3 / 1000 / 420 / 50.0 % PEG 1.5K / 26 / % / 520 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 1.0 M HEPPS pH 8.5 / 60 / mM / 60
L1 / 7 / 1000 / 300 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 4.0 M Na Acetate pH 7.0 / ### / mM / 600 / EMPTY / 0 / 0 / 1.0 M BTP pH 7.0 / 100 / mM / 100
L2 / 4.5 / 1000 / 600 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 5.0 M NH4Cl / ### / mM / 300 / EMPTY / 0 / 0 / 1.0 M Na Acetate pH 4.5 / 100 / mM / 100
L3 / 4.5 / 1000 / 200 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 5.0 M NH4Cl / ### / mM / 700 / EMPTY / 0 / 0 / 1.0 M Na Acetate pH 4.5 / 100 / mM / 100
L4 / 7 / 1000 / 200 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 5.0 M NH4Cl / ### / mM / 700 / EMPTY / 0 / 0 / 1.0 M BTP pH 7.0 / 100 / mM / 100
L5 / 8.5 / 1000 / 100 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 4.0 M NaCl / ### / mM / 800 / EMPTY / 0 / 0 / 1.0 M Tris pH 8.5 / 100 / mM / 100
L6 / 7 / 1000 / 462.5 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 1.6 M Na3 Citrate / 700 / mM / 437.5 / EMPTY / 0 / 0 / 1.0 M BTP pH 7.0 / 100 / mM / 100
L7 / 8.5 / 1000 / 150 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 1.6 M Na3 Citrate / ### / mM / 750 / EMPTY / 0 / 0 / 1.0 M Tris pH 8.5 / 100 / mM / 100
L8 / 4.5 / 1000 / 25 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 4.0 M Na Formate / ### / mM / 875 / EMPTY / 0 / 0 / 1.0 M Na Acetate pH 4.5 / 100 / mM / 100
L9 / 7 / 1000 / 25 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 4.0 M Na Formate / ### / mM / 875 / EMPTY / 0 / 0 / 1.0 M BTP pH 7.0 / 100 / mM / 100
L10 / 7 / 1000 / 150 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 8.0 M NH4NO3 / ### / mM / 750 / EMPTY / 0 / 0 / 1.0 M BTP pH 7.0 / 100 / mM / 100
L11 / 4.5 / 1000 / 328.6 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 7.0 M NaNO3 / ### / mM / 571.4 / EMPTY / 0 / 0 / 1.0 M Na Acetate pH 4.5 / 100 / mM / 100
L12 / 8.5 / 1000 / 328.6 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 7.0 M NaNO3 / ### / mM / 571.4 / EMPTY / 0 / 0 / 1.0 M Tris pH 8.5 / 100 / mM / 100
M1 / 9 / 1000 / 560 / 50.0 % PEG 1.5K / 16 / % / 320 / EMPTY / 0 / 0 / 2.8 M MgSO4 / 56 / mM / 20 / EMPTY / 0 / 0 / 1.0 M BTP pH 9.0 / 100 / mM / 100
M2 / 9.1 / 1000 / 220 / 50.0 % PEG 1.5K / 30 / % / 600 / EMPTY / 0 / 0 / 3.0 M TMACl / 240 / mM / 80 / EMPTY / 0 / 0 / 1.0 M BTP pH 9.0 / 100 / mM / 100
M3 / 4.2 / 1000 / 383.3 / 40.0 % MEPEG 2K / 18 / % / 450 / EMPTY / 0 / 0 / 3.0 M TMACl / 200 / mM / 66.67 / EMPTY / 0 / 0 / 1.0 M Succinate pH 4.0 / 100 / mM / 100
M4 / 5.3 / 1000 / 300 / 40.0 % MEPEG 2K / 26 / % / 650 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 1.0 M NaAcetate pH 5.0 / 50 / mM / 50
M5 / 5.4 / 1000 / 200 / 40.0 % MEPEG 2K / 24 / % / 600 / EMPTY / 0 / 0 / 1.6 M Na3 Citrate / 160 / mM / 100 / EMPTY / 0 / 0 / 1.0 M Na Acetate pH 4.5 / 100 / mM / 100
M6 / 5.3 / 1000 / 300 / 40.0 % MEPEG 2K / 20 / % / 500 / EMPTY / 0 / 0 / 2.0 M Li2SO4 / 200 / mM / 100 / EMPTY / 0 / 0 / 1.0 M MES/Acetate pH 5.5 / 100 / mM / 100
M7 / 5.8 / 1000 / 540 / 40.0 % MEPEG 2K / 16 / % / 400 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 1.0 M MES pH 6.0 / 60 / mM / 60
M8 / 5.8 / 1000 / 150 / 40.0 % MEPEG 2K / 26 / % / 650 / EMPTY / 0 / 0 / 2.0 M Glycine / 200 / mM / 100 / EMPTY / 0 / 0 / 1.0 M MES pH 6.0 / 100 / mM / 100
M9 / 6.3 / 1000 / 420 / 40.0 % MEPEG 2K / 18 / % / 450 / EMPTY / 0 / 0 / 2.8 M MgSO4 / 84 / mM / 30 / EMPTY / 0 / 0 / 1.0 M BisTris pH 6.5 / 100 / mM / 100
M10 / 6.5 / 1000 / 150 / 40.0 % MEPEG 2K / 26 / % / 650 / EMPTY / 0 / 0 / 2.0 M K Glutamate / 200 / mM / 100 / EMPTY / 0 / 0 / 1.0 M BisTris pH 6.5 / 100 / mM / 100
M11 / 7.2 / 1000 / 150 / 40.0 % MEPEG 2K / 28 / % / 700 / EMPTY / 0 / 0 / 2.0 M CaCl2 / 100 / mM / 50 / EMPTY / 0 / 0 / 1.0 M HEPES pH 7.5 / 100 / mM / 100
M12 / 6.7 / 1000 / 360 / 40.0 % MEPEG 2K / 16 / % / 400 / EMPTY / 0 / 0 / 2.0 M KNO3 / 280 / mM / 140 / EMPTY / 0 / 0 / 1.0 M MOPS pH 7.0 / 100 / mM / 100
N1 / 4.5 / 1000 / 547.6 / 60.0 % DMPEG 500 / 4 / % / 66.67 / EMPTY / 0 / 0 / 3.5 M (NH4)2SO4 / ### / mM / 285.7 / EMPTY / 0 / 0 / 1.0 M Na Acetate pH 4.5 / 100 / mM / 100
N2 / 5 / 1000 / 431.4 / 50.0 % PEG 400 / 2 / % / 40 / EMPTY / 0 / 0 / 3.5 M (NH4)2SO4 / ### / mM / 428.6 / EMPTY / 0 / 0 / 1.0 M NaAcetate pH 5.0 / 100 / mM / 100
N3 / 6 / 1000 / 254.3 / 50.0 % PEG 400 / 3 / % / 60 / EMPTY / 0 / 0 / 3.5 M (NH4)2SO4 / ### / mM / 585.7 / EMPTY / 0 / 0 / 1.0 M MES pH 6.0 / 100 / mM / 100
N4 / 6.5 / 1000 / 421.4 / 60.0 % DMPEG 500 / 3 / % / 50 / EMPTY / 0 / 0 / 3.5 M (NH4)2SO4 / ### / mM / 428.6 / EMPTY / 0 / 0 / 1.0 M BisTris pH 6.5 / 100 / mM / 100
N5 / 7 / 1000 / 581 / 60.0 % DMPEG 500 / 2 / % / 33.33 / EMPTY / 0 / 0 / 3.5 M (NH4)2SO4 / ### / mM / 285.7 / EMPTY / 0 / 0 / 1.0 M BTP pH 7.0 / 100 / mM / 100
N6 / 7.5 / 1000 / 234.3 / 50.0 % PEG 400 / 4 / % / 80 / EMPTY / 0 / 0 / 3.5 M (NH4)2SO4 / ### / mM / 585.7 / EMPTY / 0 / 0 / 1.0 M HEPES pH 7.5 / 100 / mM / 100
N7 / 4.6 / 1000 / 633.3 / 6.0 M 1,6-hexanediol / 1000 / mM / 166.67 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 1034: 100.0 mM Co (II) Chloride / 10 / mM / 100 / 1.0 M Na Acetate pH 4.5 / 100 / mM / 100
N8 / 4.6 / 1000 / 100 / 50.0 % PEG 400 / 30 / % / 600 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 1035: 500.0 mM Cd Chloride / 100 / mM / 200 / 1.0 M Na Acetate pH 4.5 / 100 / mM / 100
N9 / 5.6 / 1000 / 195.2 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 3.5 M (NH4)2SO4 / ### / mM / 571.4 / 1015: 1.5 M Na/K tartrate / 200 / mM / 133 / 1.0 M Na3Citrate pH 5.5 / 100 / mM / 100
N10 / 5.6 / 1000 / 695 / 25.0 % Ethyleneimine polymer / 2 / % / 80 / EMPTY / 0 / 0 / 4.0 M NaCl / 500 / mM / 125 / EMPTY / 0 / 0 / 1.0 M Na3Citrate pH 5.5 / 100 / mM / 100
N11 / 5.6 / 1000 / 600 / 50.0 % Jeffamine M-600 / 10 / % / 200 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 1036: 100.0 mM Fe (III) Chloride / 10 / mM / 100 / 1.0 M Na3Citrate pH 5.5 / 100 / mM / 100
N12 / 5.6 / 1000 / 483.3 / 6.0 M 1,6-hexanediol / 2500 / mM / 416.67 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 1.0 M Na3Citrate pH 5.5 / 100 / mM / 100
O1 / 7.3 / 1000 / 350 / 40.0 % MEPEG 2K / 20 / % / 500 / EMPTY / 0 / 0 / 1.6 M Na3 Citrate / 80 / mM / 50 / EMPTY / 0 / 0 / 1.0 M HEPES pH 7.5 / 100 / mM / 100
O2 / 7.2 / 1000 / 380 / 40.0 % MEPEG 2K / 20 / % / 500 / EMPTY / 0 / 0 / 2.0 M MgCl2 / 40 / mM / 20 / EMPTY / 0 / 0 / 1.0 M HEPES pH 7.5 / 100 / mM / 100
O3 / 8.1 / 1000 / 427.1 / 40.0 % MEPEG 2K / 18 / % / 450 / EMPTY / 0 / 0 / 3.5 M (NH4)2SO4 / 80 / mM / 22.86 / EMPTY / 0 / 0 / 1.0 M Triethanolamine pH 8.0 / 100 / mM / 100
O4 / 8.2 / 1000 / 250 / 40.0 % MEPEG 2K / 22 / % / 550 / EMPTY / 0 / 0 / 2.0 M KCl / 200 / mM / 100 / EMPTY / 0 / 0 / 1.0 M Triethanolamine pH 8.0 / 100 / mM / 100
O5 / 8.2 / 1000 / 200 / 40.0 % MEPEG 2K / 20 / % / 500 / EMPTY / 0 / 0 / 2.0 M KNO3 / 400 / mM / 200 / EMPTY / 0 / 0 / 1.0 M HEPPS pH 8.5 / 100 / mM / 100
O6 / 8.6 / 1000 / 400 / 40.0 % MEPEG 2K / 16 / % / 400 / EMPTY / 0 / 0 / 2.0 M Li2SO4 / 200 / mM / 100 / EMPTY / 0 / 0 / 1.0 M CHES pH 9.0 / 100 / mM / 100
O7 / 4.3 / 1000 / 100 / 50.0 % .PEP 426 / 36 / % / 720 / EMPTY / 0 / 0 / 4.0 M NaCl / 320 / mM / 80 / EMPTY / 0 / 0 / 1.0 M Na Acetate pH 4.5 / 100 / mM / 100
O8 / 6.4 / 1000 / 20 / 50.0 % .PEP 426 / 36 / % / 720 / EMPTY / 0 / 0 / 2.0 M KNO3 / 320 / mM / 160 / EMPTY / 0 / 0 / 1.0 M PIPES pH 6.5 / 100 / mM / 100
O9 / 7.6 / 1000 / 100 / 50.0 % .PEP 426 / 36 / % / 720 / EMPTY / 0 / 0 / 4.0 M NaCl / 320 / mM / 80 / EMPTY / 0 / 0 / 1.0 M Triethanolamine pH 7.5 / 100 / mM / 100
O10 / 5.3 / 1000 / 140 / 50.0 % .EEP 797 / 30 / % / 600 / EMPTY / 0 / 0 / 2.0 M AmSCN / 320 / mM / 160 / EMPTY / 0 / 0 / 1.0 M NaAcetate pH 5.0 / 100 / mM / 100
O11 / 6.6 / 1000 / 250 / 50.0 % .EEP 797 / 30 / % / 600 / EMPTY / 0 / 0 / 2.0 M CaCl2 / 100 / mM / 50 / EMPTY / 0 / 0 / 1.0 M MOPS pH 7.0 / 100 / mM / 100
O12 / 8.1 / 1000 / 250 / 50.0 % .EEP 797 / 30 / % / 600 / EMPTY / 0 / 0 / 2.0 M MgCl2 / 100 / mM / 50 / EMPTY / 0 / 0 / 1.0 M HEPPS pH 8.5 / 100 / mM / 100
P1 / 6.5 / 1000 / 500 / 30.0 % PEG 20,000 / 12 / % / 400 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 1.0 M MES pH 6.5 / 100 / mM / 100
P2 / 6.5 / 1000 / 200 / 50.0 % Jeffamine M-600 / 30 / % / 600 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 1037: 500.0 mM Cs Chloride / 50 / mM / 100 / 1.0 M MES pH 6.5 / 100 / mM / 100
P3 / 6.5 / 1000 / 285.7 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 3.5 M (NH4)2SO4 / ### / mM / 514.3 / 1034: 100.0 mM Co (II) Chloride / 10 / mM / 100 / 1.0 M MES pH 6.5 / 100 / mM / 100
P4 / 6.5 / 1000 / 442.9 / 70.0 % MEPEG 550 / 25 / % / 357.14 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 754: 100.0 mM ZnSO4 / 10 / mM / 100 / 1.0 M MES pH 6.5 / 100 / mM / 100
P5 / 7.5 / 1000 / 357.1 / 75.0 % MPD / 30 / % / 400 / EMPTY / 0 / 0 / 3.5 M (NH4)2SO4 / 500 / mM / 142.9 / EMPTY / 0 / 0 / 1.0 M HEPES pH 7.5 / 100 / mM / 100
P6 / 7.5 / 1000 / 500 / 50.0 % Jeffamine M-600 / 20 / % / 400 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 1.0 M HEPES pH 7.5 / 100 / mM / 100
P7 / 7.5 / 1000 / 490 / 40.0 % PEG 8K / 10 / % / 250 / 1550: 50.0 % Ethylene glycol / 8 / % / 160 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 1.0 M HEPES pH 7.5 / 100 / mM / 100
P8 / 7.5 / 1000 / 233.3 / 30.0 % PEG 10,000 / 20 / % / 666.67 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 1.0 M HEPES pH 7.5 / 100 / mM / 100
P9 / 8.5 / 1000 / 300 / EMPTY / 0 / 0 / EMPTY / 0 / 0 / 2.0 M Li2SO4 / ### / mM / 500 / 1038: 100.0 mM Ni (II) Chloride / 10 / mM / 100 / 1.0 M Tris pH 8.5 / 100 / mM / 100
P10 / 8.5 / 1000 / 231.4 / 50.0 % Glycerol / 12 / % / 240 / EMPTY / 0 / 0 / 3.5 M (NH4)2SO4 / ### / mM / 428.6 / EMPTY / 0 / 0 / 1.0 M Tris pH 8.5 / 100 / mM / 100
P11 / 9 / 1000 / 589.3 / 70.0 % MEPEG 550 / 20 / % / 285.71 / EMPTY / 0 / 0 / 4.0 M NaCl / 100 / mM / 25 / EMPTY / 0 / 0 / 1.0 M Bicine pH 9.0 / 100 / mM / 100
P12 / 0 / 1000 / 670 / 50.0 mM HDTMA Br / 10 / mM / 200 / EMPTY / 0 / 0 / 4.0 M NaCl / 500 / mM / 125 / 717: 2.0 M MgCl2 / 10 / mM / 5 / EMPTY / 0 / 0

1