Table S1. The amino acid (aa) identity of novel picornavirus strain newt/II-5-Pilis/2014/HUN (KX463670) to the corresponding regions of representative picornaviruses. The highest aa values are bolded. The identity calculation was carried out using the SIAS web server.

Genus / Type / Strain / Acc. num. / newt/II-5-Pilis/2014/HUN
(KX463670)
Coding region / P1 / P2 / P3
"Rafivirus" / Tortoise rafivirus A1 (TRaV-A1) / tortoise/UF4/USA/2009 / KJ415177 / 34.1 / 37.5 / 29.8 / 40.2
Dicipivirus / Canine picodicistrovirus 1 (CaPdV-1) / 209/Hong Kong/2008 / JN819202 / 31.8 / 28.2 / 29.1 / 37.8
Gallivirus / Chicken gallivirus 1 (ChGV-1) / chicken/518C/Hong Kong/2010 / KF979337 / 34.1 / 28.9 / 39.1 / 41
Gallivirus A1 (GV-A1) / turkey/M176/2011/HUN / JQ691613 / 34.2 / 29 / 37.9 / 39.9
Hunnivirus / Hunnivirus A1 (HuV-A1) / BHUV1/2008/HUN / JQ941880 / 29.8 / 30.9 / 27.8 / 31.3
Kobuvirus / Canine kobuvirus 1 (CaKV-1) / dog/AN211D/USA/2009 / JN387133 / 37.7 / 35.8 / 37.1 / 42.9
Feline kobuvirus 1 (FeKV-1) / 12D240/South Korea/2012 / KJ958930 / 38.4 / 38 / 39.3 / 43.1
Murine kobuvirus / KoV-SewKTM (Nepal/2009) / JQ898342 / 36.5 / 33.9 / 37.8 / 41.9
Cattle kobuvirus 1 (CKV-1) / cattle/Kagoshima-2-24-KoV/2015/JPN / LC055960 / 36.9 / 35.7 / 36.4 / 41.2
Murine kobuvirus 1 (MuKV-1) / M-5/USA/2010 / JF755427 / 37.6 / 36.5 / 36.7 / 42.8
Caprine kobuvirus (CapKV) / black goat/12Q108/KOR/2012 / KF793927 / 36.9 / 36.6 / 37.2 / 43.6
Bovine kobuvirus 1 (BKV-1) / bov/U-1/Japan / AB084788 / 37.3 / 37.5 / 37 / 42.5
Cattle kobuvirus 2 (CKV-2) / cattle/Kagoshima-1-22-KoV/2014/JPN / LC055961 / 37.1 / 37.5 / 37.5 / 41
Feline kobuvirus 1 (FeKV-1) / FK-13/South Korea/2011 / KF831027 / 38.2 / 37.6 / 37.7 / 42.8
Porcine kobuvirus 1 (PKV-1) / sw/S–1–HUN/2007 / EU787450 / 37.6 / 37.6 / 39.4 / 43.7
wb/WB-1-HUN/2011 / JX177612 / 38 / 37.6 / 39 / 43.9
European roller kobuvirus 1 (ERKV-1) / SZAL6-KoV/2011/HUN / KJ934637 / 37.4 / 37.8 / 38.2 / 42.6
Aichi virus 1 (AiV-1) / A846/88 / AB040749 / 38.1 / 37.9 / 37.9 / 42.4
Feline kobuvirus 1 (FeKV-1) / TE/52/IT/13 / KM091960 / 38 / 37.9 / 39.1 / 42.5
Ferret kobuvirus (FKV) / ferret/MpKoV38/NL/2010 / KF006985 / 37.1 / 38.4 / 36.9 / 42.9
Ovine kobuvirus 1 (OKV-1) / sheep/TB3/HUN/2009 / GU245693 / 37.6 / 38.5 / 38.3 / 42.2
Megrivirus / Chicken megrivirus (ChMV) / ch/CHK-IV-CHV/2013/HUN / KF961187 / 29.9 / 25.4 / 24.2 / 35.8
ch/B21-CHV/2012/HUN / KF961186 / 29.7 / 25.6 / 24.1 / 36
ch/QIA01/SKR/2003 / KJ690629 / 29.8 / 25.7 / 24.1 / 35.9
Mesivirus 1 (MeV-1) / pigeon/HK-21/2011/HKN / KC876003 / 30.2 / 25.8 / 26.4 / 35.7
Chicken megrivirus (ChMV) / ch/27C/Hong Kong / KF979336 / 29.9 / 25.8 / 34.6 / 36.3
Duck megrivirus (DuMV) / du/LY/China / KC663628 / 29.5 / 26.7 / 23.5 / 35.1
Turkey hepatitis virus 1 (THV-1) / turkey/B407-THV/2011/HUN / KF961188 / 29.4 / 26.8 / 22.5 / 36.4
chicken/5C/Hong kong / KF979335 / 30.3 / 27 / 34.2 / 36.2
Mesivirus 2 (MeV-2) / pigeon/GALII-5/2011/HUN / KC811837 / 30.3 / 27.3 / 26 / 35.8
Oscivirus / Oscivirus A2 / thrush/Hong Kong/10878/2006 / GU182410 / 40 / 35.2 / 34.3 / 49.4
Oscivirus A1 / robin/Hong Kong/10717/2006 / GU182408 / 39.2 / 36.7 / 32.3 / 50.1
Passerivirus / Passerivirus A1 (PasV-A1) / thrush/Hong Kong/00356/2007 / GU182406 / 34.5 / 33 / 38.4 / 40.2
thrush/Hong Kong/00805/2007 / GU182407 / 34.6 / 33 / 38.4 / 40.4
Rosavirus / Rosavirus A2 (RoV-A2) / hu/GA7403/Gambia/2008 / KJ158169 / 32.5 / 24.9 / 34.3 / 37
Rosavirus A1 (RoV-A1) / mouse/USA/M-7/2010 / JF973686 / 32.1 / 25.1 / 34.2 / 36
Sakobuvirus / sakobuvirus A1 (SakV-A1) / FFUP1/Portugal/2012 / KF387721 / 37.7 / 35.6 / 35.9 / 42.5
Salivirus / Salivirus A1 (SaV-A1) / hu/NG-J1/Nigeria/2007 / GQ179640 / 36.4 / 36.6 / 34.7 / 39.6
Sicinivirus / sicinivirus A1 (SiV-1) / chicken/UCC001/Eire / KF741227 / 30.2 / 31.3 / 36.2 / 38
unassigned siciniviruses / chicken/55C/Hong Kong/2008 / KF979331 / 33.2 / 32 / 34.5 / 37.3
Unassigned / Rabbit picornavirus (RabPV) / rabbit01/2013/HUN / KT325852 / 36.9 / 37 / 37.5 / 41.7
Unassigned / bat kobu-like virus BtMf-PicoV-2 (BKLV-BtMf-PicoV-2) / BtMf-PicoV-2/GD2012 / KJ641691 / 39.4 / 38.4 / 39.6 / 43.9
Unassigned / bat kobu-like virus BtMr-PicoV (BKLV-BtMr-PicoV) / BtMr-PicoV/JX2010 / KJ641686 / 38.5 / 39.6 / 40.1 / 43.2

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