Intro, Background, Purpose/Hypothesis
Other Research done at Wofford
Spring 2006 – 1st meal, rats eat more fruitloops than Funyuns or rat chow
Pick 2 Rolls papers that relate to ours the best and present their results
Intermittent access to sugar paper – relation to restricted access of 5 FL daily
Sensory specific satiety explanation
Binge eating discussion
Binge eating is eating a certain percentage of food?
Define Binge eating
DSM IV criteria
Discuss binge eating as an eating disorder (bulimia) and obesity
Stressful situation paper – cause of binge eating?
what else causes binge eating?
Rats as animal model for binge eating
why rats are used in experimenting eating disorders?
common lab animal
Purpose
to determine if restricted access to highly palatable food will promote binge eating
Hypothesis
Daily restricted access to highly palatable food triggers binge eating
Materials & Methods
Materials
rat chow – normal food
fruit loops – sweet food (treat) – highly palatable food
male Sprague Dauley rats – explanation?
Methods
12 hr light/dark cycle
weekly care – fresh water
5 FL for limited access group daily - counterbalanced
0 FL daily for no access group – counterbalanced
2 group types: intermittent access and no access
- 3 experimental groups
- RC – RC
- RC – FL
- FL – RC
Procedures (time line)
7:00 – move into testing environment, remove bedding
meal #1meal #2 meal #2
placedplacedweighed
aboveaboveand returned
cagecageto cage
______
7:59 8:009:009:29 9:3010:00 10:3010:50
meal #1meal #1 meal #1 meal #2
placed weighedremoved & weighed
in cageand returnedweighed weigh rats & place
to cagemeal #2 inserted in clean cages
Other powerpoint ideas:
materials – pics of rats, food items
Picture of 12 hr light/dark cycle
Handling of neophobicity
- Acclimatization for 1 hour
- Bowl above cage for sniffing
- Bowls were inserted in cages before the cycle of experiments started for them to get used to the presence of the bowl
Results & Conclusions
Does intermittent access to highly palatable food induce binge eating after satiation?
Average minutes of latency: meal 1 vs. meal 2 (between each group)
weekly weight gain of rats
Average amount of food intake: meal 1 vs. meal 2 (each group presented)
Do rats binge eat on highly palatable food or rat chow?
Bar graph of 1st hour of experiment showing food intake for each group
Add meal 2nd hour – meal 2 on top of bars (show 2nd with animation)
Discussion/Significance/Relevance/Implications
Tie in all results to reach a conclusion
Are rats an animal model for binge eating?
Types of highly palatable foods that humans binge on:
desserts after meals – society serves desserts (highly palatable food last in meal, this could increase the chance of an unecessary intake of large amounts of calories)
RC – FL paradigm
holiday meals (variety/buffet style)
How does our experiment relate to humans
SSS – binge eating – eating disorders – obesity
Can use results to find possible new treatments (to obesity, sugar addiction, etc)
Limitations
Do these foods represent comparable foods to a human diet?
Is food restriction a type of stress?
Do they become accustomed to having FL?
Is 5 FL enough or is it too much?
No FL-FL condition – don’t really know what results would be even though can speculate
Neophobicity – should be counterbalanced out
Further studies:
Vary amount of FL